scholarly journals KOPI DAPAT MENURUNKAN AKTIVITAS ALT DAN AST SERUM DARAH TIKUS WISTAR ALKOHOLIK

2018 ◽  
Vol 6 (2) ◽  
pp. 56
Author(s):  
I Made Sara Wijana ◽  
I Gede Suranjaya ◽  
Iriani Setyawati
Keyword(s):  
Body Weight ◽  
Data Analysis ◽  
Correlation Analysis ◽  
Blood Serum ◽  
Correlation Coefficient ◽  
Negative Control ◽  
Albino Rats ◽  
Treatment Groups ◽  
Wistar Albino Rats ◽  
Alt Activity

Ethanol can damage the liver, indicated by an increase of alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in the blood of alcohol-treated rats. Coffee is rich in antioxidants which are hepatoprotector agent. This study aimed to determine the effect of coffee to reduce alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in blood serum of alcohol-treated rats. The study used 32 male wistar albino rats (200±20g  weight) which were divided into four treatment groups i.e. P0 (negative control which were treated with 1 mL of 30% ethanol/200g body weight (bw)/day), P1 (1 mL of 30% ethanol/200 g bw/day + coffee 0,1g/200g bw in 3,5 mL of water/200 g bw/day), P2 (1 mL of 30% ethanol/200g bw + coffee 0,15 g/200g bw in 3,5 mL of water/200g bw/day), dan P3 (1 mL of 30% ethanol/200g bw + coffee 0,2g/200g bw in 3,5 mL of water/200g bw/day). ALT and AST levels were measured by spectrophotometric method. Data analysis was done by ANOVA followed with LSD test and correlation analysis. The results showed that coffee reduced (P<0.01) the activities of ALT and AST of rats blood serum. ALT and AST activities were significantly different (P<0.01) among treatments and with control. Dose of coffee was negatively correlated with ALT activity with correlation coefficient (R) -0.978 and AST with correlation coefficient (R) -0.985 (P<0,01). The lowest of ALT and AST activities were found at dose of coffee 0.2 g/200g body weight. Keywords: ethanol, ALT, AST, coffee  

scholarly journals Body Weight and Platelet Count Changes in Zidovudine Administered Wistar Albino Rats Treated with Ethanolic Extracts of Annona muricata and Fagara zanthoxyloide

2019 ◽  
pp. 1-18
Author(s):  
Ekere, Oghenekaro Uchechukwu ◽  
Ikpeama, Utomoibor Evelyn
Keyword(s):  
Body Weight ◽  
Platelet Count ◽  
Normal Control ◽  
Negative Control ◽  
Fixed Dose ◽  
Control Group ◽  
Albino Rats ◽  
Annona Muricata ◽  
Ethanolic Extracts ◽  
Wistar Albino Rats

Zidovudine administration either in single or fixed-dose combination usually results in a decrease in body weight and in some cases thrombocytopenia. The present study investigated the body weight and platelet count changes observed in zidovudine pre-administered Wistar albino rats on treatment with ethanolic extracts of the leaves Annona muricata (AM) and roots of Fagara zanthoxyloide (FZ). Plants samples were collected from Alakahia community, Rivers state and Opoo community, Ogun state; while Wistar albino rats were grouped into normal control, negative control (receiving zidovudine at 100 g/ml/Kg bw), AM and FZ extract treatment (at 4.5 and 3.8 g/ml/Kg B.W respectively) groups with analysis performed bi-weekly. All tests were performed using standard procedures with all reagents of analytic grade. Phytochemical screening of the extracts showed significantly high amounts of alkaloids (10.47- 21.15 mg / 100 g), phenols (10.60-15.22 mg / 100 g) and tannins (14.23 - 50.19 mg / 100 g). The investigation into their proximate compositions also showed high fat (5.78 ± 0.02) in FZ, moisture (10.47 ± 0.05) in AM and fibre (7.37 ± 0.03) in FZ. The amino acid phenylalanine (5.10-6.01 g / 100 g), isoleucine (4.44-5.20 g / 100 g), lysine (4.03 -5.31 g / 100 g) was observed to be available in the extracts. In the normal control group body weight increased by 20.75 g at week 6 while administration of Zidovudine, resulted in a decreased in bodyweight by 1.14 g in week 2 and 3.32 g in week 6. Extract treatment caused a significant increase (p≤0.05) in body weight by 17.95g (AM) and 18.23g (FZ) at week 6. Platelet count was also observed to significantly decrease (p≤0.05) by 33.42% in the negative control group when compared to the normal control. This was observed to significant increases in extract treatment by 49.56% (AM) and 51.32% (FZ). The results thus suggest a possible beneficial effect of the extracts of AM and FZ in checkmating the weight and platelet loss observed as a side effect of zidovudine therapy as well as the possible use in haemorrhagic conditions to reduce bleeding without thromboembolism.

scholarly journals To Evaluate the Diuretic Activity in Ethanolic Extract of Leaves of Delonix Regia in Wistar Albino Rats

2018 ◽  
Vol 11 (2) ◽  
pp. 959-963
Author(s):  
Jayanthi M K ◽  
Siddamma Amoghimath
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Diuretic Activity ◽  
Delonix Regia ◽  
Group 4 ◽  
Group 2 ◽  
Wistar Albino Rats

To study the diuretic activity in ethanolic extract of leaves of delonix regia in wistar albino rats. After obtaining the permission from the Institutional Ethical Committee (IAEC) Wistar albino rats were randomly selected and divided into 4 groups. The animals were fasted and deprived of food and water 20hrs prior to the experiment. On the day of experimentation, Group 1 negative control received Normal saline (25ml/kg), Group 2 positive control received Furosemide 20mg/kg body weight, and Group 3 received ethanonic extract of delonix regia 100mg/kg and Group 4 received ethanonic extract of delonix regia 200 mg/kg. Delonix regia increase the excretion of sodium and water at the dose of 100mg/kg and 200mg/kg of body weight. The diuretic index is 5.93 and 6.45 at 100mg/kg and 200mg/kg of delonix regia respectively. The diuretic activity if delonix regia 100mg/kg is 0.61 and 0.67 at dose 200mg/kg, which is more than that of negative control but less when compared to positive control. Ethanonic extract of Delonix regia showed significant diuretic activity at both 100mg/kg and 200mg/kg doses.

scholarly journals Effects of Aqueous Extract of Vigna unguiculata Seedlings Against Doxorubicin-Induced Cardiotoxicity and Kidney damage In Female Albino Rats

2016 ◽  
Vol 1 (04) ◽  
Author(s):  
Olatunde A. Oseni ◽  
Ademola S. K. Idowu ◽  
Aminat Isah
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Vigna Unguiculata ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Enzyme Markers

Doxorubicin is an anthracycline drug which is believed to cause immediate damage to myocardial cells by free radical generation in the cause of treatment of cancer. This study was however aimed to investigate the effects of the aqueous extract of the seedlings of Vigna unguiculata on heart, kidney as well as lipid profile disorders caused by this drug on female Wistar albino rats. Fifteen female Wistar albino rats were divided into three groups. Group 1 animals served as normal positive control; Group 2 animals served as negative control which were treated with 0.5mL of (20mg/kg body weight doxorubicin) while Group 3 animals were treated with 0.5mL each of 20mg/kg body weight doxorubicin and 10% aqueous extract of seedlings of Vigna unguiculata. Some enzyme markers and lipid contents were determined. The results of the study showed an increase in the activities of plasma ALP and AST after treatment with the drug except in the heart for AST which showed a significant reduction, while treatment with the extract brought about a decrease in the plasma and the organs except for kidney AST. The ALT on the other hand showed slight increase in the plasma with a decrease in the kidney and heart after treatment with the drug as the treatment with the extract tend to restore it to the control in both cases, there was increased plasma and kidney but reduced heart HDL-C after treatment with the drug which was observed to be restored to control after treatment with extract in both situations. The triglyceride and total cholesterol did not show similar trend in the plasma and the studied organs. The LDL-C was also observed to be increased in both plasma and organs after the treatment with the drug which later reduced significantly towards the control after the administration of the sprout extract. The present study has shown that the doxorubicin has damaging effects on both kidney and heart tissues while the sprouts extract produce a restoration to the normal in both organs.

scholarly journals HEPATOPROTECTIVE ACIVITY OF THE ETHANOLIC EXTRACT OF DOG FRUIT RIND (Pithecellobium lobatum Benth.)

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Ruqiah Ganda Putri Panjaitan ◽  
Ely Savitri ◽  
Titin Titin
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Alanine Transaminase ◽  
Albino Rats ◽  
Aspartate Transaminase ◽  
Wistar Albino Rats ◽  
Fruit Rind

The present study was conducted to investigate the hepatoprotective activity of ethanolic extract of dog fruit rind (Pithecellobium lobatum benth.) against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. Male Wistar albino rats were randomly divided into four groups and administered orally with 50 mg/200 g body weight of dog fruit rind extract (K1), 100 mg/200 g body weight (K2) of dog fruit rind extract, 5 mg/200 g body weight of silymarin (K3/positive control), and 0.4 mL/200 g body weight of distilled water (K4/negative control), for seven days The levels of alanine transaminase (ALT) of each K1; K2; K3; and K4 were 143.40±83.75 U/L, 94.80±93.77 U/L, 130.20±58.54 U/L and 147.25±107.97 U/L, respectively, while the aspartate transaminase (AST) levels were 304.20±128.67 U/L; 213.20±88.93 U/L; 333.00±128.31 U/L; and 239.25 ± 94.90 U/L, respectively (P>0.05). Group K2 showed better histological pattern than other groups with 60% of mild and 40% of moderate liver damage. Our findings revealed the hepatoprotective activity of the ethanolic extract of dog fruit rind.

scholarly journals Blood glucose lowering effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes), Daucus carota subsp. Sativus (carrot) extracts on lead induced toxicity in albino wistar rats

2021 ◽  
Vol 14 (1) ◽  
pp. 065-070
Author(s):  
Ekpe IP ◽  
Eze KA ◽  
Amaechi Dennis
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Solanum Lycopersicum ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Solanum Melongena ◽  
Negative Control ◽  
Albino Rats ◽  
Solanum Lycopersicon ◽  
Treatment Groups

Lead is one of the heavy metal humans are often expose to either through food, cosmetics or environment. This study is aimed at investigating the positive effect of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes) and Daucus carrots subsp.sativus (carrot) on blood glucose concentration, body weight, and feeding habits of albino rats on lead toxicity. In this study 35 albino rats weighing 80-120g were grouped into five. Group 1 served as normal control, group 2 negative control. The remaining three groups served as treatment groups 1, 2 and 3. All groups except group 1 (normal control) were administered 50mg/kg of lead acetate. Treatment groups were administered 200mg/kg of Solanum melongena (garden egg), Solanum lycopersicum (tomatoes) and Daucus carrots subsp.sativus (carrot). Group 3 was administered Solanum malongen and Daucus carrots subsp.sativus while group 4 was given Daucus carrots subsp.sativus and solanum lycopersicon. Group 5 was administered Solanum malongen, Daucus carrots subsp.sativus and Solanum lycopersicon. Changes in blood glucose concentration, body weight, feed and water consumption recorded at intervals. The result showed a significant decrease (P<0.05 and P<0.01) in blood glucose concentration in treatment groups compared to negative control. There was also significant increase (P>0.05, P>0.01 and P>0.001) in body weight and feed consumed in treatment groups compared to negative control. Combined therapy of any two of Solanum malongen, Daucus carrots subsp.sativus and Solanum lycopersicon juice can help in the management of hyperglycemia and reverse internal abnormality or injury resulting in weight loss in lead induced toxicity.

scholarly journals Hematological Changes and Antidiabetic Activities of Colocasia esculenta (L.) Schatt Stem Tuber Aqueous Extract in Alloxan Induced Diabetic Rats

2020 ◽  
pp. 1-9
Author(s):  
Nwaogwugwu, Joel . Caleb ◽  
Uhegbu, F. O. ◽  
Okereke, S. C. ◽  
Nosiri, C. I. ◽  
Amaka, E. N. ◽  
...  
Keyword(s):  
Body Weight ◽  
Negative Control Group ◽  
Negative Control ◽  
Diabetic Rats ◽  
Colocasia Esculenta ◽  
Diabetic Group ◽  
Control Group ◽  
Albino Rats ◽  
Treatment Groups ◽  
Tuber Extract

Management of blood glucose level is the hallmark in the treatment of diabetes. Much work has not been done on the management of diabetes using the stem tuber extract of Colocasia esculenta. The objective of this study was to evaluate the antihyperglycemic and hematological parameter on Colocasia esculenta aqueous stem extract in alloxan induced diabetic rats. Sixty (60) male rats were used in the study. Seven days of acclimatization, the rats were divided randomly into six groups of five in each group. Group 1: Served as normal control, Group 2: Diabetic control group (negative control), Group 3: Diabetic group and “Glucinorm-M80” (positive control), Group 4: Diabetic group and extract at 200 mg/kg bodyweight, Group 5: Diabetic group and extract at 400 mg/kg, Group 6: Diabetic group and extract at 600 mg/kg. Diabetes was induced in albino rats by intraperitoneal injection of alloxan at a single dose of 120 mg/kg body weight in groups 2 to 6 after starving them for 24 hrs. The animals were given feed and water ad libitum. The albino rats were administered for twenty eight days with the aqueous Colocasia esculenta stem tuber, after which they were fasted overnight, anaesthetized with chloroform and sacrificed. The result showed that there was a significant increase (p<0.05) in mean body weight of the positive control and the treatment groups (200 mg/kg to 600 mg/kg) when compared with the negative control which has a significant decrease (p<0.05) in mean body weight. The result also showed a significant increase (P<0.05) in the concentrations of RBC, PCV, HB, while PLT and MCH showed significant decrease (P<0.05) in the treatment groups and negative control group when compared with the normal control group. Also there was a no significant (P<0.05) difference in MCHC of the treatment groups when compared with the control group and negative control group. Also there was a significant difference in glycosylate hemoglobin of the treatment groups when compared with the control group and negative group. This study has demonstrated that aqueous stem tuber extract of Colocasia esculenta has a significant increase on body weight which may have a role of improving the states of possible weight loss following complicated diabetes. Also, aqueous stem tuber extract of Colocasia esculenta has an ameliorative effect on sugar level and some hematological parameters of alloxan induced diabetic rats showing effective diabetic control and management of diabetes.

scholarly journals Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Raghavendra Mitta ◽  
Sushmitha Duddu ◽  
Raghuveer Yadav Pulala ◽  
Pradeepkumar Bhupalam ◽  
Venkatakirankumar Mandlem ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Sodium Fluoride ◽  
Total Protein ◽  
Reduced Glutathione ◽  
Serum Biomarkers ◽  
Albino Rats ◽  
Serum Total Protein ◽  
Treatment Groups ◽  
Group I

AbstractObjectivesThe main objective of the present study is to evaluate the mitigative effect of hydroalcoholic extract of Momordica cymbalaria fruits against sodium fluoride (NaF) induced hepatotoxicity.MethodsIn this study, Wistar male albino rats were randomly divided into five groups of six rats each. Group I and II served as normal and toxic controls. Group III as plant control received extract at a dose of 400 mg/kg b. wt, p.o and Groups IV and V as treatment groups received extract at a dose 200 and 400 mg/kg b. wt, p.o for 30 days. All groups except Groups I and III received 100 ppm of NaF through drinking water. After completion of the study, blood collected for the estimation of liver blood serum biomarkers such as aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline Phosphatase (ALP), direct and total bilirubin, total protein and albumin. The liver tissue homogenate was for estimation of lipid peroxidation, catalase, and reduced glutathione levels.ResultsThe results showed that NaF intoxication caused elevation of liver blood serum levels and lipid peroxidation; decreased levels of serum total protein, albumin and liver reduced glutathione, and catalase observed. The treatment groups showed decreased elevated serum biomarkers (ALT, AST, and ALP), liver lipid peroxidation and increased serum total protein and albumin, liver reduced glutathione and catalase levels in a dose-dependent manner. Histopathological studies also further strongly supported for mitigative effects of the plant.ConclusionsIn conclusion, our findings of the study indicated that M. cymbalaria fruits were a potential drug candidate in the treatment of NaF induced hepatotoxicity.

scholarly journals Effect of Carica papaya L. Stem Bark Extracts on Cholesterol Concentration in Rats Induced with Streptosozin

2020 ◽  
Vol 151 ◽  
pp. 01011
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Carica Papaya ◽  
Stem Bark ◽  
Negative Control ◽  
Diabetic Rats ◽  
Cholesterol Concentration ◽  
Bark Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Stem Bark Extract

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.

scholarly journals The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues

2021 ◽  
Vol 9 (1) ◽  
pp. 22-25
Author(s):  
Asmaa Saad Mahmoud Shokhba ◽  
Mohamed A. Abdel-Rahman ◽  
Mohammed Alaa El-Deen A. Omran ◽  
Nahla Soliman El-Shenawy
Keyword(s):  
Body Weight ◽  
Vascular Permeability ◽  
Saline Solution ◽  
Public Awareness ◽  
Control Group ◽  
Albino Rats ◽  
Colorimetric Determination ◽  
Crude Venom ◽  
Treatment Groups ◽  
Renal Vascular

Background: Among venomous elapid snakes, cobras have the highest public awareness, as their venom represents a combination of proteins, peptides, and enzymes that have a range of biochemical and pharmacological roles and are also the main constitutes of biological activity and lethal toxicity. Objectives: The study aimed to evaluate the effect of the venom of Egyptian Spitting Cobra, Naja nubiae, on the vascular permeability based on the extravasation of the azo dye Evans blue (EB) into the tissues of the liver and kidneys of animals envenomed with low (¼ LD50; 0.32 mg/kg) and high (½ LD50; 0.65 mg/ kg) doses at three sampling times (30, 120, 360 min) post-injection of the venom. Methods: Fifty-four adult male Albino rats (8 weeks old and 180±2 0 g body weight) were divided into three main groups (n=6). In the control group, rats were subcutaneously (SC) injected with saline solution. Envenomed groups were SC injected, one group with 0.32 mg/kg and the other group with 0.65 mg/kg body weight of crude venom, respectively. Rats were I.V injected with EB dye 20 minutes before SC injection with saline solution as control animals and with Naja nubiae venom as treatment groups. Results: The results illustrated a high significant rate of EB extravasation to hepatic and renal tissues by the colorimetric determination of EB dye concentration. Conclusion: The venom of Naja nubiae can cause increased hepatic and renal vascular permeability which may explain the inflammatory effect induced by this venom.

scholarly journals BIOCHEMICAL EVALUATION OF CHRONIC CONSUMPTION OF MONOSODIUM GLUTAMATE ON LIVER OF WISTAR ALBINO RATS

2021 ◽  
pp. 99-102
Author(s):  
SURENDRA BABU THANGACHI ◽  
VARSHA SRIRAM MOKHASI ◽  
SHABINA KOMATH CHENOLY
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Total Bilirubin ◽  
Liver Enzymes ◽  
Monosodium Glutamate ◽  
Density Lipoprotein ◽  
Lipid Profiles ◽  
Albino Rats ◽  
High Dose ◽  
Wistar Albino Rats

Objective: The objective of this study was to determine if there were any harmful effects of monosodium glutamate (MSG) on the liver of Wistar albino rats chronically at three different doses, namely, low, mid, and high doses equivalent to human consumption doses in developing countries. Methods: The Wistar albino rats (n=24) were divided into four groups, namely control, Low dose MSG (180 mg/kg), Mid dose MSG (360 mg/kg), and High dose MSG (720 mg/kg). At the end of the experimental period (120 days), animal blood was collected retro-orbitally to analyze the liver enzymes such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), Total protein, Albumin, and Total Bilirubin in blood serum. Lipid profiles, namely, Triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and Total cholesterol were subjected to analysis using blood serum. Results: Significant increase (p<0.05) in AST, ALT, ALP, and total bilirubin in serum of MSG induced low, mid, and high dose groups when compared to control group were recorded. There was a significant increase (p<0.05) in LDL, decrease in HDL, increase in total cholesterol and triglycerides of MSG-induced animal groups. Conclusion: The effects of MSG on serum liver enzymes and lipid profiles in this present animal study were not severely alarming even though the dosage was chronic which opens further discussion on the controversies revolving around MSG.

Sign in / Sign up

Export Citation Format

Share Document