scholarly journals Histochemical Localization of Bio-macromolecules in Cestode (Raillietina sp.) infected country fowls (Gallus domesticus) in India

2021 ◽  
Vol 8 (4) ◽  
pp. 457-460
Author(s):  
N C Patra ◽  
Arkaprava Mandal ◽  
S Pradhan ◽  
R N Hansda ◽  
Samiran Mondal ◽  
...  

In Indian GDP, veterinary sector plays a major role in contribution wherein poultry meat plays good protein source. But the health status is less studied in case of country fowl.Indian country fowl are highly infected with producing emaciation, reduced growth rate, highly morbidity with enteritis.After confirmation by gross and microscopy, samples undergone histopathology study using Haematoxylin and Eosin stain (H&E). Thereafter, the samples also processed for detection of carbohydrate by McManus method using PAS stain and mucoprotein was detected using congo red method.Adult possesses carbohydrate, protein and fat. Histologically the mature, immature and gravid segments were differentiated whereas the histochemical techniques qualifies the biomacromolecules. The lipid droplet showed a clear vacuole preferably in and around the periphery portion. The glycogen distributed in an even manner. Subsequently, the protein was deposited mostly at the periphery in the body surfaces as well as in the egg.

2018 ◽  
Vol 28 (1) ◽  
pp. 23 ◽  
Author(s):  
Priyono Priyono ◽  
Atien Priyanti

Human Development Index (HDI) is used to measure overall performance where Indonesia's HDI is ranked 110 out of 187 countries with an index value of 0.684 in 2015. Consumption of animal protein has a relationship with life expectancy and quality of life which determines by the HDI performance. The paper describes on perspective development of animal protein source from livestock in Indonesia. The production development of animal protein source in overall (meat, eggs, and milk) had a positive growth during the period of 1994-2015 with the range of 1-6%/year, except that for buffalo meat production that decreased by 1.77%/year. The animal protein availability of meat was dominated by broiler production, followed by beef, mutton and others. The broiler meat production has the highest growth rate of 6.67%/year. Meanwhile, the animal protein source of non-meat production was dominated by eggs and milk production that its growth rate were 5.98 and 4.29%/year, respectively. In the same period, the meat production share was dominated by poultry meat, the rest derived from large ruminant, small ruminant, and other meat sources. Egg and milk production share also increased significantly during the period and share availability of beef production was less than 25% where its consumer participation was decreased. This indicates that beef is not a primary consumption for the majority people of Indonesia. Development program is needed to encourage an increase of the protein source from poultry meat, large ruminants, small ruminants, and milk.


Author(s):  
C.D. Fermin ◽  
M. Igarashi

Otoconia are microscopic geometric structures that cover the sensory epithelia of the utricle and saccule (gravitational receptors) of mammals, and the lagena macula of birds. The importance of otoconia for maintanance of the body balance is evidenced by the abnormal behavior of species with genetic defects of otolith. Although a few reports have dealt with otoconia formation, some basic questions remain unanswered. The chick embryo is desirable for studying otoconial formation because its inner ear structures are easily accessible, and its gestational period is short (21 days of incubation).The results described here are part of an intensive study intended to examine the morphogenesis of the otoconia in the chick embryo (Gallus- domesticus) inner ear. We used chick embryos from the 4th day of incubation until hatching, and examined the specimens with light (LM) and transmission electron microscopy (TEM). The embryos were decapitated, and fixed by immersion with 3% cold glutaraldehyde. The ears and their parts were dissected out under the microscope; no decalcification was used. For LM, the ears were embedded in JB-4 plastic, cut serially at 5 micra and stained with 0.2% toluidine blue and 0.1% basic fuchsin in 25% alcohol.


2001 ◽  
Author(s):  
Candace M. Jones ◽  
Kathy Phipps ◽  
Nancy Reifel ◽  
Betty Skipper ◽  
Patrick Blahut

1992 ◽  
Vol 66 (2) ◽  
pp. 100-107 ◽  
Author(s):  
V. G. M. Swarnakumari ◽  
R. Madhavi

ABSTRACTFifty day-old chicks were each infected with 10 excysted metaccreariae of Philophthalimus nocturnus Looss. 1907 around each orbit and growth, development and allometry were studied. The growth rate showed two phases over a period of 35 days, a limited lag phase lasting two days post-infection in which flukes did not exceed 440 μm in length, and a rapid phase during which growth was rapid and flukes reached a size of 3·008–3·504 mm on day 35. Five developmental stages were noticed during the course of development of the metacercaria to the egg-producing adult stage. Eggs appeared in the uterus on day 14 and oculate miracidia on day 25. The hindhody, testes and ovary showed positive allometric growth, the pharnyx less so, whereas negative allometric growth was shown by the forebody. Body width, oral sucker and ventral sucker were close to isometry, growing at the same rate as the body length.


1980 ◽  
Vol 94 (1) ◽  
pp. 95-104 ◽  
Author(s):  
G. J. Rowlands

SummaryBlood samples were taken at 9, 10 and 11 weeks of age from 230 male Hereford × Friesian calves, the progeny of 12 Hereford bulls. Concentrations of blood glucose, serum albumin, inorganic phosphate and sodium were measured and correlated with body weights and growth rates until slaughter at 19½ months of age.Correlations between growth rates and glucose concentrations (0·44) and between growth rates and albumin concentrations (0·38) were observed until 4 months of age. Similar correlations between body weights and blood glucose or albumin concentrations persisted until 6 months of age. By 10 months, however, the correlations had begun to decline, and by slaughter they had become insignificant.Correlations between growth rates and inorganic phosphate or sodium concentrations were smaller, and also decreased with age.The effect of hypoglycaemia on growth rate was compared with the effect of enzootic pneumonia. Although the 10% of calves with the lowest glucose concentrations were growing 24% more slowly than the other calves at the time of sampling, this growth depression was not related to pneumonia, and they subsequently made up for most of the early loss of growth.Three blood samples were also taken from 22 Hereford bulls. No significant correlations were observed between the blood composition of the bulls and the body weights or weight gains of their progeny.


2021 ◽  
Author(s):  
Fei Xie ◽  
Xue Jiang ◽  
Yang Yi ◽  
Zi-Jia Liu ◽  
Chen Ma ◽  
...  

Abstract The potential for preventive and therapeutic applications of H2 have now been confirmed in various disease. However, the effects of H2 on health status have not been fully elucidated. Our previous study reported changes in the body weight and 13 serum biochemical parameters during the six-month hydrogen intervention. To obtain a more comprehensive understanding of the effects of long-term hydrogen consumption, the plasma metabolome and gut microbiota were investigated in this study. Compared with the control group, 14 and 10 differential metabolites (DMs) were identified in hydrogen-rich water (HRW) and hydrogen inhalation (HI) group, respectively. Pathway enrichment analysis showed that HRW intake mainly affected starch and sucrose metabolism, and DMs in HI group were mainly enriched in arginine biosynthesis. 16S rRNA gene sequencing showed that HRW intake induced significant changes in the structure of gut microbiota, while no marked bacterial community differences was observed in HI group. HRW intake mainly induced significant increase in the abundance of Lactobacillus, Ruminococcus, Clostridium XI, and decrease in Bacteroides. HI mainly induced decreased abundances of Blautia and Paraprevotella. The results of this study provide basic data for further research on hydrogen medicine. Determination of the effects of hydrogen intervention on microbiota profiles could also shed light on identification of mechanism underlying the biological effects of molecular hydrogen.


2021 ◽  
Vol 888 (1) ◽  
pp. 012072
Author(s):  
Siti Khairunnisa ◽  
Sumiati ◽  
Cece Sumantri ◽  
Wiwin Winarsih

Abstract This study aimed to evaluate the effect of zinc supplementation in various diets on the performance and health status of IPB-D2 chickens. Subsequently, about 180 one-week-old IPB-D2 chickens were used in the study and were fed the treatment diets for 12 weeks. Also, this study employed a completely randomised factorial design with six treatments and three replications of ten birds each. These treatment diets included A1, which contained nutrients based on SNI (2013) without Zn supplementation, A2 which is a combination of A1 and 60 ppm of Zinc, B1 which contained nutrients 5% higher than SNI without Zn supplementation, B2 which is a combination of B1 and 60 ppm Zinc, C1 which contains nutrients 10% higher than SNI without Zn supplementation, and lastly, C2 which is a combination of C1 and 60 ppm of Zinc. Furthermore, the data were analysed using ANOVA, and the parameters observed were the body weight, body weight gain, feed consumption, feed conversion ratio, blood profile and the villi surface area of the jejunum. Consequently, the results showed that C2 significantly (P<0.05) increased the body weight and body weight gain when fed to the finisher and grower chickens, respectively. More so, when the finisher chickens were fed the 10% SNI diet, there was an increased body weight gain and also a significantly (P<0.05) decreased feed conversion ratio. Furthermore, the addition of 60 ppm zinc increased haemoglobin significantly (P<0.05), whereas C2 decreased leukocytes significantly (P<0.05) in chicken blood. However, the treatments had no effect on feed consumption in the villi surface area of the jejunum. Therefore, the study concluded that supplementing the IPB-D2 chickens with 60 ppm zinc in a 10% SNI diet resulted in the best performance and health status.


2019 ◽  
Vol 20 (11) ◽  
Author(s):  
ARDO TANJUNG ◽  
H.T.S.S.G. SARAGIH ◽  
TRIJOKO ◽  
H.P. SOENARWAN ◽  
S. WIDIANTO ◽  
...  

Abstract. Tanjung A, Saragih HTSSG, Trijoko, Soenarwan HP, Widianto S, Mahardhika IWS, Daryono BS. 2019. Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758). Biodiversitas 20: 3207-3212. An experiment was conducted to detect SNP of the myostatin gene and its association with the body weight of hybrid chicken crossbreed from F1 Kamper and BC1 Broiler. Four F1 Kamper hens were crossbred with BC1 Broiler rooster. Day old chick (DOC) hatched were maintained for 49 days with body weight measurement every seven days. The blood samples from 49 days old chicken were taken for DNA isolation by Chelex 5% method and then amplification of the myostatin gene. PCR products were sequenced, and sequence alignment was performed using Clustal Omega to obtain SNP. The SNP obtained was analyzed by the Pearson correlation test with the body weight of forty nine-days-old chickens. The body weight of the hybrid chicken is higher than of Pelung chicken but lower than the Broiler. There are 7 SNPs in myostatin gene exons included 2 Adenine insertions, 1 Guanine deletion, and four substitutions (C2244G, G2283A, T4842G, G7378T) that yield nine haplotypes. Six haplotypes had different protein sequences with Myostatin protein, while three haplotypes were identical to Myostatin protein. The correlation analysis showed that there was a strong positive correlation (r = 0.736) between normal Myostatin protein and mutant to chicken body weight at 49-days-old. Adenine insertion to nucleotide 2099-2100 of myostatin gene had a very strong positive correlation (r = 0.800) to 49-days-old chicken body weight, although T4842G substitution had a strong negative relationship (r = -0.773) to 49-days-old chicken body weight. Adenine insertion to nucleotide 2099-2100 of myostatin gene could be a genetic marker of heavier body weight of the hybrid chicken.


2020 ◽  
Vol 64 (3) ◽  
pp. 132-138
Author(s):  
Anastasiya A. Khablova ◽  
Galina N. Bondar’ ◽  
Pavel F. Kiku ◽  
Natal’ya S. Tumanova ◽  
Valentina N. Rasskazova ◽  
...  

Human health, in addition to social and spiritual well-being, is determined by its physical condition, which reflects the functional capabilities of the body, features of physical development and ensures working capacity in any activity, including academic workload, which is extremely important for students in higher educational institutions. The purpose of the study is to assess the health status of students at the School of Biomedicine of the Far Eastern Federal University. Material and methods. The health status of 184 medical students (147 young women and 37 young men) was assessed using a sociological survey (questionnaire) and a study of the component composition of the body using bioimpedancemetry. Results. The low physical activity, detected in 68% of students and bad habits (smoking tobacco) have been established to negatively affect students’ health. Among smokers, 75% of students live in dormitories on the university campus. According to the results of bioimpedancemetry, significant deviations from the age norm were noted in terms of body mass index (BMI) (50% of cases) and fat mass (FM) (71% of cases). Significant gender differences in the individual components of the body were not identified. Discussion. The most aggressive factors that significantly affect the health of students are unbalanced nutrition, lack of compliance with the regime of study and rest, lack of sleep and stay in the fresh air, lack of physical activity, lack of motivation for a healthy lifestyle. Significant deviations in BMI and FM, identified by bioimpedancemetry, indicate the risks of developing arterial hypertension, metabolic syndrome, and nutritional status disorders. Medical students need to give recommendations for increasing motor activity and changing diet. Conclusion. The results of the study can be used in the future to determine the functional state of students and develop preventive measures to preserve the health of medical students.


2020 ◽  
Vol 20 (2) ◽  
pp. 117
Author(s):  
Eko Harianto ◽  
Eddy Supriyono ◽  
Tatag Budiardi ◽  
Ridwan Affandi ◽  
Yani Hadiroseyani

Aquaculture is is often constrained by the availability of water, both in quality and quantity. The aim of this study was to determine the optimum maintenance system of elver (Anguilla bicolor bicolor) on a wet, moist and dry system through production performance studies and physiological responses. The research was conducted from August to September 2018 at the Production Technic and Management of Aquaculture Laboratory, Department of Aquaculture, IPB University. A completely randomized design with 5 treatments and 3 replications was used in this study, namely treatment with high water wet system 0% of body height (A), high water wet system 50% of body height (B), high water wet system 100% of body height (C), moist system (D), and dry system (E). The body weight of elver was 18.80 ± 0.62 g which derived from fish cultivator in Bogor, West Java. Fish was fed 3 times per day at satiation with commercial feed which a protein content of 50%. Results showed that treatment C was the best result with survival rate of 100%, the specific growth rate of 0.45% day -1, the absolute growth rate of 0.07 g day 1 and feed conversion ratio of 6.57. The analysis of variance showed that the treatments showed the significant effect (P <0.05). The blood glucose value closest to the eel condition before treatment was found in the treatment C.


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