scholarly journals Antimicrobial Evaluation of Natural Spices (Tumeric, Garlic, Onion, Celery and Cinnamon) Versus Synthetic Antibiotics (Tetracycline, Clindamycin and Ceftriazone) at Three Different Concentrations (10%, 50% and 100%)

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
RC Jagessar ◽  
F Ali ◽  
G Shrikant ◽  
M Astwood
Keyword(s):  
Herbal Medicines ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Synthetic Drugs ◽  
Microbial Inhibition ◽  
A Value ◽  
Antimicrobial Evaluation ◽  
Aseptic Conditions ◽  
The Cost ◽  
Diffusion Assay

Most Synthetic drugs have several side effects when administered and the cost of synthesizing them is an expensive endeavour. One alternative is to use herbal medicines. Guyana has an abundance of diverse flora which has been receiving attention in this area. However, much more research needs to be done. The antibacterial activity of natural spices (Tumeric, garlic, onion, celery and cinnamon) was investigated against E. coli, S. aureus, K. pneumoniae and P. aeruginosa and was compared with synthetic antibiotics (Tetracycline, Clindamycin and Ceftriazone) at three different concentrations of 10%, 50% and 100%. The Disc Diffusion Assay was used to investigate the antimicrobial potency of the synthetic drugs and herbal medicines under aseptic conditions. The synthetic drugs were prepared at different concentrations of 10%, 50% and 100%, whereas the extract was investigated at 100% concentration. Experiments were done in triplicates and the diameter of zone of microbial inhibition (DZOI) was measured and expressed as the mean with standard deviation (SD). The precision of comparisons (e.g. 95% confidence intervals) and Area of Zone of Inhibition, AZOI was also computed. Selected microorganisms were also tested against a reference standard antibiotic, Ampicillin and Nystatin. For the synthetic drugs, the highest Area of Zone of Inhibition, AZOI of 1566.4 mm2 was induced by Clindamycin against S. aureus at 50% concentration whereas the lowest AZOI of 245.09 mm2 was induced by P. auriginosa at 10% concentration. For the herbal extracts, the highest AZOI of 1256.6 mm2 was induced by the onion extract versus K. pneumonia whereas the lowest AZOI of 50.3 mm2 was induced by the Tumeric extract against E. coli. Antimicrobial selectivity was also observed for both synthetic and herbal medicines. For example, for tetracycline against S. aureus, AZOI of 1093.92 mm2 was obtained whereas against P. auriginosa, AZOI of 530.67 mm2 was observed. Garlic exhibited AZOI of 139.49 mm2 against E. coli whereas a value of 78.5 mm2 was obtained against P. aeruginosa.  

scholarly journals Synthesis, Molecular Docking and Antimicrobial Evaluation of Some Benzothiazoles

2021 ◽  
Author(s):  
Smita J. Pawar ◽  
Amol Kale ◽  
Priya Zori ◽  
Rahul Dorugade
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Property ◽  
Docking Study ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Good Antibacterial Activity ◽  
Comparable Activity ◽  
Antimicrobial Evaluation ◽  
Bacteria And Fungi

Abstract Abstract The new series of 2-(substituted amino)-N-(6- substituted-1,3-benzothiazol-2yl) acetamide BTC(a-t) has been synthesized by appropriate synthetic route from substituted 2-amino benzothiazole. The synthesized compounds were screened experimentally for its antimicrobial property against gram positive, gram negative bacteria and fungi. Zone of inhibition and minimum inhibitory concentration of compounds was determined against selected bacterial and fungal strains. Compound BTC-j N-(6-methoxy-1,3-benzothiazol-2-yl)-2-(pyridine-3-yl amino) acetamide and compound BTC-r N-(6-nitro-1,3-benzothiazol-2-yl)-2-(pyridine-3-yl amino) acetamide found to have good antimicrobial potential. The compound BTC-j has shown good antibacterial activity against S. aureus at MIC of 12.5 µg/ml, B. subtilis at MIC of 6.25µg/ml, E. coli at MIC of 3.125µg/ml and P. aeruginosa at MIC of 6.25µg/ml. No statistical difference in antimicrobial activity of standard and test compounds was found indicating test compounds have comparable activity. Further docking study was carried out to check the probable interactions with the selected protein using V-life MDS 3.5 software. (DNA gyrase, PDB: 3G75). The dock score of compounds and antimicrobial activity found to be consistent.

scholarly journals Antimicrobial and anti-oxidant potential of Periploca hydaspidis

2015 ◽  
Vol 10 (3) ◽  
pp. 645 ◽  
Author(s):  
Rafi Ullah ◽  
Jehan Bakht ◽  
Mohammad Shafi
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Methanolic Extract ◽  
Maximum Growth ◽  
Zone Of Inhibition ◽  
Aqueous Fraction ◽  
E Coli ◽  
Methanolic Extracts ◽  
Anti Oxidant ◽  
Diffusion Assay

<p>The present study investigates antimicrobial and anti-oxidant potential of different solvent extracted samples from <em>Periploca hydaspidis </em>through disc diffusion assay. The data revealed that all the extracts reduced the growth of the tested microbes using three different concentrations. <em>Pseudomonas aeruginosa </em>and <em>E. coli</em> were sensitive to crude extracts and all fractions measuring varying degree of growth inhibition.  Similarly, the growth of <em>Citrobacter freundii</em> was not inhibited by crude methanolic extracts, n-hexane, ethyl acetate and aqueous extracted samples. Maximum growth inhibition was measured against <em>Klebsiella pneumoniae</em> exposed to aqueous fraction followed by n-hexane fraction. Our results also suggested that among various fungi, <em>Candida albicans</em> was sensitive to crude methanolic extracts, n-hexane and aqueous fractions compared with other fractions. The rest of the fungi under test were resistant to crude and all fractions showing no zone of inhibition. All the extracted samples of the stems and roots showed antioxidant activity, however, crude methanolic extract of stem tissues exhibited better antioxidant activity than the other extracts.</p>

scholarly journals Physicochemical, phytochemical and antibacterial studies of few medicinal plants of Visakhapatnam region against bacterial clinical isolates

2016 ◽  
Vol 5 (03) ◽  
pp. 1296
Author(s):  
Phani Kumari Uddandapu* ◽  
K. Chandrasekhara Naidu ◽  
Y. Venkateswar Rao
Keyword(s):  
Medicinal Plants ◽  
Herbal Medicines ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Anticancer Properties ◽  
Antimicrobial Efficiency ◽  
Antimicrobial Studies ◽  
Methanolic Extracts ◽  
Minimum Zone ◽  
Ethnic Uses

The present study was conducted to evaluate the physicochemical and preliminary phytochemical studies, antimicrobial studies on sixteen medicinal plants collected in and around regions of Visakhapatnam district using methanol as a solvent. Phytochemical screening revealed that methanolic extracts, contained, steroids, alkaloids, phenol, flavonoids, polyphenols and glycosides. The physicochemical parameters like ash and extractive values were determined. The highest percentage of total ash, was found in Catharanathus roseus. The extractive values can be used as a reliable aid for detecting quality of drug adulteration. Antimicrobial efficiency of studies plants was tested against four bacterial clinical pathogens including Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The diameter of zone of inhibition (ZOI) was measured and it was found that C. roseus showed maximum zone of inhibition against gram+ve S. aureus and minimum zone of inhibition against gram–ve E. coli. Also the zone of inhibition was increased on increasing concentration. Results observed in the present study help to distinguish the plant extracts from its adulterants and quality and it may be indicative of the methanol extracts of studied plants could be a possible source to obtain new and effective herbal medicines to treat infections, which justify the ethnic uses of C. roseus against various infectious diseases apart from its anticancer properties.

Synthesis, Characterization and in vitro Antimicrobial Evaluation of Pyrazole Based Oxothiazolidine Hybrids

2020 ◽  
Vol 5 (4) ◽  
pp. 295-300
Author(s):  
Yogesh J. Sanghani ◽  
Suresh B. Koradiya ◽  
Krushnakumar J. Jilariya
Keyword(s):  
Diffusion Method ◽  
Spectral Studies ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method ◽  
Mass Spectral ◽  
E Coli ◽  
Antifungal Potential ◽  
Antimicrobial Evaluation ◽  
Antibacterial And Antifungal

In this work, pyrazole based oxothiazolidine hybrids, 4-{4-[2-(1- phenyl-3-(substituted)phenyl-1H-pyrazol-4-yl)-4-oxo-thiazolidin-3- yl]-phenyl}-morpholin-3-one (11a-l) were synthesized using molecular hybridization approach through Vilsmeier-Haack reaction. The titled compounds 11a-l were characterized by elemental analysis, IR, 1H NMR and mass spectral studies. The antibacterial activity of 11a-l was evaluated in vitro by agar cup plate method against B. cocous, B. subtillis, E. coli and P. vulgaris. The antifungal activity of compounds 11a-l was evaluated in vitro by agar based disk diffusion method against A. niger. The results of antibacterial and antifungal evaluation were reported in terms of zone of inhibition measured in mm. The synthesized compounds 11a-l exhibited moderate to good antibacterial and antifungal potential. Compound 4-{4-[2-(1-phenyl-3-(2-methoxyphenyl) phenyl-1H-pyrazol-4-yl)-4-oxo-thiazolidin-3-yl]-phenyl}- morpholin-3-one (11h) emerged as a most potent antimicrobial agent displaying zone of inhibition 21, 20, 21, 24 and 20 mm against B. cocous, B. subtillis, E. coli, P. vulgaris and A. niger, respectively.

scholarly journals Antimicrobial Activity of the Ethanolic and Aqueous Extract of Vicia faba L. (Fabaceae) at Three Different Concentrations in the Absence and Presence of Zn(OAc)2.2H2O

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
RC Jagessar ◽  
S Hope ◽  
S Issacs ◽  
Y Crepaul
Keyword(s):  
Antimicrobial Activity ◽  
Vicia Faba ◽  
Aqueous Extract ◽  
Metal Salt ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
Passiflora Edulis ◽  
E Coli ◽  
Vicia Faba L ◽  
Diffusion Assay

The aqueous and ethanolic extract of Passiflora edulis and Vicia faba L. (Fabaceae) exhibited antimicrobial activity against the pathogenic microorganisms: E. coli, S. aureus, K. pneumoniae and C. albicans. This was evaluated using the Disc Diffusion Assay under asceptic conditions. Antimicrobial activity wasn’t induced by the solvent, ethanol nor water. The highest AZOI was 153.9 mm2 and the lowest 12.56 mm2. Negligible Zone of Inhibition, ZOI were observed in several instances. The aqueous extract of the fruit also induced negligible Zone of Inhibition, ZOI. In comparison to the reference, Ampicillin and Nystatin, these values are less. As the concentration of the metal salt, Zn(OAc)2.2H2O and ethanolic extract increases, there seem to be a variation in antimicrobial activity. Zn(OAc)2.2H2O appears to intensify the antimicrobial activity of the plant ethanolic and aqueous extract. Zn (OAc)2.2H2O in the absence of any extracts exhibited antimicrobial activity. The AZOI range from 47.2 mm2 to 117.8 mm2. Antimicrobial selectivity was also observed in several instances.

scholarly journals Anti-Bacterial Analysis of Some Herbal Medicines in Mbale, Eastern Uganda

2020 ◽  
pp. 28-33
Author(s):  
H. A. Aliyu ◽  
A. M. Danjuma ◽  
K. Abubakar
Keyword(s):  
Escherichia Coli ◽  
Herbal Medicine ◽  
Random Sampling ◽  
Herbal Medicines ◽  
Antibacterial Activities ◽  
Bacterial Activity ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Eastern Uganda

This study was aimed at investigating the anti-bacterial activity of some on shelf herbal medicines for treatment of Cholera and Typhoid in markets. The sample was collected from shops that sell herbal medicines using random sampling to investigate the effectiveness of these herbal medicines on Escherichia coli and Salmonella enterica serotype typhi. It was noticed that all the four herbal medicines that were subjected to antibacterial activities using E. coli and S. typhi, showed effect on the bacteria. Diameters of zones of inhibition was measured, which showed that zone of inhibition varies among the on shelf herbal medicines. Serial dilution was also carried out to check for the effect of concentration. Herbal Medicine for Typhoid 1 showed the highest zone of inhibition 14.36 mm and the lowest zone of inhibition was Herbal Medicine for Cholera 1 with 10.17mm on S. Typhi. While for E. coli, the highest zone of inhibition was 12.83mm shown by Herbal Medicine for Cholera 2 and the lowest was 10.25mm shown by Herbal Medicine for Typhoid 1.

REKAYASA NILAI HARGA PEMBUATAN TERHADAP EFEKTIFITAS PENGGUNAAN BAHAN PADA PEMBANGUNAN PERUMAHAN TIRTO PENATARAN ASRI TIPE 70/135 KECAMATAN NGLEGOK KABUPATEN BLITAR

2017 ◽  
Vol 7 (1) ◽  
pp. 43-52
Author(s):  
Mochamad Tamim Ma’ruf
Keyword(s):  
Engineering Application ◽  
Cost Savings ◽  
Comparison Method ◽  
Value Engineering ◽  
Design Work ◽  
Housing Projects ◽  
Initial Design ◽  
A Value ◽  
The Cost ◽  
Design Proposal

One-solving methods and techniques necessary to avoid inefficiencies and not economic costs as well as reduce the cost of housing construction is the method of Value Engineering. Value engineering is a method and cost control techniques to analyze a function to its value at the lowest cost alternative (most economical) without reducing the quality desired.At the writing of this study used a comparison method by comparing the initial design to the design proposal of the author. In the housing projects Upgrading Tirto Penataran Asri type 70, the application of Value Engineering conducted on the job a couple walls and roofs pair by replacing some work items with a more economical alternative but does not change the original function and high aesthetic level and still qualify safe. For that performed the step of determining a work item, the alternative stage, the analysis stage, and the stage of recommendations to get a Value Engineering application and cost savings against the wall a couple of work items and partner roof.The proposed design as compared to the initial design. Work items discussed was the work of a couple wall having analyzed obtained savings of Rp. 2,747,643.56 and the work of the roof pair obtained savings of Rp. 2,363,446.80. Thus the total overall savings gained is Rp 5,111,090.36 or savings of 0048%.

scholarly journals Nephrotoxicity of Herbal Products in Europe—A Review of an Underestimated Problem of Nephrotoxicity of Herbal Products

2021 ◽  
Vol 22 (8) ◽  
pp. 4132
Author(s):  
Katarzyna Kiliś-Pstrusińska ◽  
Anna Wiela-Hojeńska
Keyword(s):  
Kidney Diseases ◽  
Herbal Medicines ◽  
Kidney Injury ◽  
Herbal Products ◽  
Chronic Kidney Diseases ◽  
European Origin ◽  
Synthetic Drugs ◽  
The Many ◽  
Pharmacologically Active ◽  
Traditional Chinese Herbal Medicines

Currently in Europe, despite the many advances in production technology of synthetic drugs, the interest in natural herbal medicines continues to increase. One of the reasons for their popular use is the assumption that natural equals safe. However, herbal medicines contain pharmacologically active ingredients, some of which have been associated with adverse effects. Kidneys are particularly susceptible to injury induced by toxins, including poisonous constituents from medicinal plants. The most recognized herb-induced kidney injury is aristolochic acid nephropathy connected with misuse of certain Traditional Chinese herbal medicines. Data concerning nephrotoxicity of plant species of European origin are scarce. Here, we critically review significant data of the nephrotoxicity of several plants used in European phytotherapy, including Artemisia herba-alba, Glycyrrhiza glabra, Euphorbia paralias, and Aloe). Causative mechanisms and factors predisposing to intoxications from the use of herbs are discussed. The basic intention of this review is to improve pharmacovigilance of herbal medicine, especially in patients with chronic kidney diseases.

scholarly journals Legal Complexities of Animal Welfare in Australia: Do On-Animal Sensors Offer a Future Option?

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 91
Author(s):  
Jaime Manning ◽  
Deborah Power ◽  
Amy Cosby
Keyword(s):  
Animal Welfare ◽  
Data Transmission ◽  
Continuous Measurement ◽  
Mental Wellbeing ◽  
Value Proposition ◽  
Data Governance ◽  
Behavioural State ◽  
A Value ◽  
Welfare Measures ◽  
The Cost

The five freedoms and, more recently, the five domains of animal welfare provide internationally recognised frameworks to evaluate animal welfare practices which recognise both the physical and mental wellbeing needs of animals, providing a balanced view of their ability to cope in their environment. Whilst there are many techniques to measure animal welfare, the challenge lies with how best to align these with future changes in definitions and expectations, advances in science, legislative requirements, and technology improvements. Furthermore, enforcement of current animal welfare legislation in relation to livestock in Australia and the reliance on self-audits for accreditation schemes, challenges our ability to objectively measure animal welfare. On-animal sensors have enormous potential to address animal welfare concerns and assist with legislative compliance, through continuous measurement and monitoring of an animal’s behavioural state and location being reflective of their wellbeing. As reliable animal welfare measures evolve and the cost of on-animal sensors reduce, technology adoption will increase as the benefits across the supply chain are realised. Future adoption of on-animal sensors by producers will primarily depend on a value proposition for their business being clear; algorithm development to ensure measures are valid and reliable; increases in producer knowledge, willingness, and trust in data governance; and improvements in data transmission and connectivity.

Electrospun PU/MgO/Ag Nanofibers for Antibacterial Activity and Flame Retardency

2021 ◽  
pp. 0887302X2110094
Author(s):  
V. Mamtha ◽  
H. N. Narasimha Murthy ◽  
V. Pujith Raj ◽  
Prashantha Tejas ◽  
C. S. Puneet ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Melting Point ◽  
Protective Clothing ◽  
Electrospun Nanofibers ◽  
Synthesis And Characterization ◽  
Zone Of Inhibition ◽  
Solution Combustion ◽  
E Coli ◽  
Afterglow Time

Antibacterial activity and fire retardation are equally desired for protective clothing. For achieving this, AgNP and MgO are independently researched as nanofillers in Polyurethane based electrospun nanofibers and their synergistic effect is scarcely addressed. This article reports synthesis and characterization of MgO of 70.01 nm and AgNP of 51 to 76 nm by solution combustion and hydrothermal routes respectively and their incorporation in electrospinning of Polyurethane. Flow rate 1 ml/hr, applied voltage 13 kV, tip to collector distance 15 cm were adopted for the electrospinning. Nanofibers of 65 nm were obtained for PU/MgO (3 wt. %) and 106 nm for PU/MgO (3 wt. %)/Ag (1 wt. %). Addition of MgO increased the melting point, after flame time and afterglow time. Incorporation of AgNP improved antibacterial activity. PU/MgO/Ag (2 wt. %) exhibited zone of inhibition of 2.1 cm and 3 cm against E. Coli and S. Aureus, respectively.

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