scholarly journals Histochemical changes in skin burn wound regeneration after application of titanium nickelide alloy supernatant

2018 ◽  
Vol 7 (3) ◽  
pp. 68-74
Author(s):  
R. M. Uruzbaev ◽  
V. G. Bychkov ◽  
E. A. Yuzhakova
Keyword(s):  
Titanium Nickelide ◽  
Skin Flap ◽  
Healing Process ◽  
Regenerative Process ◽  
Control Group ◽  
Water Metabolism ◽  
Burn Wound ◽  
Biosynthetic Activity ◽  
Electron Probe Analysis ◽  
Conventional Procedure

Objective : to determine the content and distribution in the tissues of the skin of various glycosaminoglycans (GAGs) against the background of the local action of the supernatant of the titanium nickelide alloy, to reveal the positive effect of the substrate on the dynamics of the formation of the regenerative process. Material and methods. The study was carried out on 25 Syrian hamsters (Mesocricetus auratus, Waterhouse, 1839). In stages 1, 3, 7, 14, 21 and 28 days, an overdose of sevoran anesthesia followed by decapitation was used to take the skin flap. The material was processed and poured into paraffin according to the standard conventional procedure. Histological sections were stained with alcian blue with a pH of 1.0 and 2.5, according to Tenzer-Unna. To determine the content of nickel and titanium cations, the electron-probe analysis method applied to the skin grafts taken in all experimental groups was used. Results. the increased accumulation of GAG in the dermis of the control group of animals occurred for a period of 21 days, while in the experiment their maximum was reached already by 14 days. Variations in GAG content in the epidermis and derivatives are associated with a change in the proliferative and biosynthetic activity of the cells of these structures at different periods of the experiment. Given the negative charge of sulfated aminoglycans and the hydrophilic base of the supernatant, penetration of the base metal cations of the supernatant into damaged tissues was detected by scanning microscopy. Conclusions. The reparative processes with the supernatant of the titanium nickelide alloy are more intensive, due to the limitation of the destructive process within the epidermis and the dermis, the reduction of purulent necrotic complications in the healing process, the interaction of nickel and titanium anions and cations in mineral and water metabolism. This ensures an earlier occurrence and uniform maturation of the young connective tissue, epithelization with normalization of cell proliferation and differentiation processes.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals The Effect of Educated Platelets on the Healing Process of Burn Wounds in Rats: Educated Platelets in Rat Burn Wound Healing

2021 ◽  
Vol 8 ◽  
Author(s):  
Orkun Ilgen ◽  
Sefa Kurt ◽  
Osman Yilmaz ◽  
Murat Celiloglu
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Healing Process ◽  
Recovery Period ◽  
Control Group ◽  
Study Group ◽  
Second Line ◽  
Burn Wound ◽  
Wound Area ◽  
Burn Wound Healing

Burns are serious life-threatening health problems. This study was performed to determine the effect of educated platelets on burn wound healing process. 28 female wistar albino 200-220 gr rats were randomly divided into four groups. Group A1 rats(n:7) were the first-line burnt group from which blood samples are extracted to develop platelet-rich plasma(PRP) with the educated platelets that have a response to burn injury. Group B1 rats(n:7) were the unburnt group with ordinary platelets. Group A2 rats(n:8) were the second-line burnt group which was given PRP with educated platelets. Group B2 rats(n:6), as control group, were the second-line burnt group which was given PRP with ordinary platelets. Photos of rats' dorsum were taken by digital camera on the first day and 21st day of the study. Wound healing was determined by scar surface area. In the study group (Group A2) mean wound area was  53±37 mm², in the control group (Group B2) mean wound area was 114±55 mm² on the last day of the experiment. The sizes of the wounded areas were significantly lower in the study group compared with the control group (p: 0.039). Educated platelets seem to facilitate the recovery period of burn wound healing in rats.

scholarly journals Reprint-The Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Survival of Random Skin Flap on Sterptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 1 (4) ◽  
pp. 01-08
Author(s):  
Farzaneh Chehelcheraghi ◽  
Khadijah Rezazadeh ◽  
Khatereh Anbari
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Skin Flap ◽  
Healing Process ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Average Weight ◽  
Diabetic Patients

Background and Objective: Wound dressing and healing in diabetic patients is encountered with many problems. This study aimed to investigate the effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on the survival of random skin flap (RSF) on Streptozotocin-induced diabetic rats (STZ) using an optical microscope. Materials & Methods: In this study, 60 male Albino Wistar rats were used (average weight 250-300 gr). The rats were divided into six groups: 1) Health-Non (HN), 2) Health-Cells (HC), 3) Health-Sham (HS), 4) Diabetic-Non (DN) that were became diabetic by injecting STZ 70 mg/kg intraperitoneally), 5) Diabetic-Sham (DS), and 6) Diabetic-Cell (DC). In all groups, the day of surgery was considered as the zero day, on the back area of animal, the flap was created with a size of 8 × 3 cm and the BM-MSCs were performed. The sampling was performed on day 7 after surgery from the region where Transitional Zone (TZ) necrosis was initiated. Results: BM-MSCs increased the number of blood vessels (P=0.009) and the histology parameters (wound demarcation P=0.0001, granulation tissue P=0.0001) significantly compared to the control group. But this increase was not significant in the area of the survival region. Conclusion: It was concluded that after treatment with BM-MSCs, the wound healing process in both non-diabetic and diabetic groups was increased in accordance with histological characteristics.

scholarly journals Topical treatment of standardised burns with herbal remedies in model rats

2005 ◽  
Vol 5 (4) ◽  
pp. 50-57 ◽  
Author(s):  
Fahir Bečić ◽  
Nedžad Mulabegović ◽  
Zakira Mornjaković ◽  
Elvedina Kapić ◽  
Senad Prašović ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Herbal Remedies ◽  
Control Group ◽  
Therapeutic Effects ◽  
Skin Area ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

scholarly journals Experimental model of the burn wound topical treatment

2003 ◽  
Vol 3 (4) ◽  
pp. 61-66
Author(s):  
Amra Čabaravdić ◽  
Irfan Zulić ◽  
Zakira Mornjaković ◽  
Mirjana Mijanović ◽  
Fahir Bečić
Keyword(s):  
Animal Model ◽  
Clinical Symptoms ◽  
Healing Process ◽  
Surrounding Tissue ◽  
Control Group ◽  
Wound Healing Process ◽  
Herbal Preparation ◽  
Burn Wound ◽  
Experimental Animals ◽  
Significant Difference

AbstractBACKGROUND AND PURPOSE:Clinical research of drugs is a researching step subsequent to the preclinical studies in experimental animals. The aim of our research was to evaluate animal model of wound healing process after the burninducement and effects of the ointment containing natural plants on the process of burn healing.MATERIAL AND METHODS:Burn wounds were experimentally induced in two species of experimental animals which were treated with topically applied herbal preparation with concomitant monitoring of the healing process. Experimental groups (1) of 15 animals each (mice and rats), while control group (2) of 10 animals each (mice and rats) that were not being treated with herbal ointment. After the hair removal, burn was induced on the back of animals by heated brass seal. Different clinical symptoms including oedema of surrounding tissue, redness, exudation, size of the burn surface, histological and microbiological findings were monitored on the days 1, 3, 7, 14 and 21. A statistically significant difference was observed throughout descriptive statistics and paired Student's t-test.CONCLUSION:Physiological healing processes of the acute burn wound following the topical application of herbal preparation can be monitored on the utilized animal model. A three-week treatment resulted in the 90% of completed epithelization in both animal species, indicating the effectiveness of topically applied herbal preparation.

The Effect of Bakiko (Spinach- Chitosan- Collagen) Membrane on Total Fibroblast in Burn Wound Grade II

2018 ◽  
Vol 15 (2) ◽  
pp. 93
Author(s):  
SHOFI IQDA ISLAMI ◽  
AL MUNAWIR ◽  
IDA SRI SURANI WIJI ASTUTI
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Collagen Membrane ◽  
Fibroblast Cells ◽  
Burn Wound ◽  
Control Group Design ◽  
One Way Anova ◽  
Homogeneous Data ◽  
Second Degree

<pre><strong><em>Background:</em></strong><em> According to WHO data in 2016, every year, about 265,000 deaths occurred due to burns and 38% of burn patients got pathological scars due to delayed wound healing. Glutamine, vitamin C, and linoleic acid in spinach play an important role in wound healing by increasing the formation of granulation tissues including increasing the proliferation of fibroblasts. Chitosan-collagen dressings can also increase the formation of granulation tissue. Increased proliferation of fibroblasts will increase extra cellular matrix synthesis so that the wound heals quicker. The purpose of this research is to explain the effect of merging Spinach-Chitosan-Collagen (Bakiko) as membrane form on total fibroblas in healing process of second degree burns. </em></pre><pre><strong><em>Methods:</em></strong><em> This research is true experimental laboratories with posttest only control group design. The samples were 27 Rattus Novergicus divided into 9 groups: 3 negative control group (without therapy), 3 positive control group (Bioplacenton), and 3 treatment groups (Bakiko membrane). Second degree burns made by contacting the skin to hot metal plates. Bioplacenton was applied daily on wounds while Bakiko membrane is attached to the wound and replaced every 3 days. The tissue retrieval was done after the termination of the animals on the 3rd, 7th, and 21st days post-burn induction. The tissue was made as histopathologic preparations with HE staining then calculated the total fibroblast cells using imageJ software. Data analysis was done using One Way Anova for homogeneous data and Kruskal Wallis for non homogeneous data. </em></pre><pre><strong><em>Result:</em></strong><em> One Way Anova and Kruskal Wallis test  showed significant results on the 3rd day (p = 0,000) and 7th day (p = 0.004). </em></pre><strong><em>Conclussion:</em></strong><em> There is an effect of Bakiko membrane (spinach- chitosan- collagen) on the total fibroblasts in second degree burns by increasing the number of fibroblast cells at 3rd and 7th day on the proliferation phase of the burn-healing process.</em>

scholarly journals EFEK EKSTRAK TANAMAN ASHITABA PADA KADAR SERUM ALKALIN FOSFATASE (ALP) PADA TIKUS YANG TERPAPAR LUKA BAKAR

2021 ◽  
Vol 5 (1) ◽  
pp. 26-34
Author(s):  
Andrew Johan ◽  
Kusmiyati Tjahjono ◽  
Wenda Yoanda ◽  
Dwi Ngestiningsih ◽  
Lusiana Batubara
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Serum Alkaline Phosphatase ◽  
Healing Process ◽  
T Test ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Control Group Design

Serum alkaline phosphatase (ALP) levels increases in burn tissue damage. Several bioactive compounds found in Ashitaba, are expected to reduce serum ALP levels and enhance the process of wound healing. This study aims to prove that administration of oral Ashitaba extract can reduce serum ALP levels in rats exposed to burn trauma. This study uses true experimental post-test control group design with a total of 20 Sprague dawley rats as samples. All samples were inflicted with 2nd degree burn wound and divided into 2 groups, treatment group (Ashitaba extract 300 mg/KgBW) and control group. Blood serum were analyzed for ALP levels on the 2nd, 8th and 14th days. Kinetic-IFCC method was used to find serum ALP levels. Data was analyzed using Mann-Whitney Test, paired T-test amd independence T-test. Scalded wound size was measured macroscopically over the course of 21 days to find contracture rate. In conclusion, Ashitaba extract is not proven to significantly reduce the serum ALP levels, increase contracture rate and enhance burn wound healing process. However, there was slight increase in contracture rate in treatment group as compared to control group. In addition, there was a lower ALP levels in treatment group as compared to control group in day 8.

scholarly journals Healing Effect of Sesame Ointment on Second-degree Burn Wound in Rats

2016 ◽  
Vol 5 (2) ◽  
pp. 56-62
Author(s):  
Setareh Tehrani ◽  
Parisa Lotfi ◽  
Sepideh Tehrani ◽  
Ehsan Jangholi ◽  
Hoda Aryan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Treated Group ◽  
Sesame Oil ◽  
Control Group ◽  
Burn Wound ◽  
Matrix Deposition ◽  
Degree Burn ◽  
Anti Inflammatory ◽  
Second Degree

Background: Wound healing requires processes like cell migration, proliferation of extracellular matrix deposition, remodeling and angiogenesis. Also, anti-oxidative and anti-inflammatory reactions play key roles in the progression of wound healing. Previous studies showed that sesame oil has anti-inflammatory, antimicrobial and antioxidant activities. In addition, it is shown that sesame oil stimulates fibroblast production in vitro and in skin wounds. Therefore, in this study we investigated the effects of sesame ointment on the process of second-degree burn wound healing in rat models according to stereological parameters. Materials and Methods: In this experimental study, forty male Wistar rats (200±20 g) were randomly divided into four groups (n=10): ointment-base treated group (vehicle), silver sulfadiazine (SDD) treated group, sesame treated group (E1) and the control group which received no treatment. A 2×3 cm2 standard second-degree burn wound was induced on the posterior surface of animal’s neck under general anesthesia. After sacrifice, the animal’s skin sample was fixed in buffered formaldehyde for stereological evaluations. The data was analyzed by SPSS statistical software (version 14.0). P<0.05 was considered statistically significant. Results: According to our results, the mean of reduction in wound areas, volume density of collagen bundles and hair folicules, fibroblast populations, length density of vessels in E1 group was significantly higher than control group (P<0.05). The differences between E1 and SSD were not statistically noticeable regarding the stereological parameters. Conclusion: According to stereological analysis, administration of sesame ointment showed the ability to improve wound healing process and tissue regeneration in the treatment of skin damages.[GMJ.2016;5(2):56-62]

scholarly journals In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masarra M. Sakr ◽  
Walid F. Elkhatib ◽  
Khaled M. Aboshanab ◽  
Eman M. Mantawy ◽  
Mahmoud A. Yassien ◽  
...  
Keyword(s):  
Survival Rate ◽  
Murine Model ◽  
Histopathological Examination ◽  
Healing Process ◽  
Bacterial Count ◽  
Treated Group ◽  
Control Group ◽  
In Vivo Evaluation ◽  
Systemic Spread

AbstractFailure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

scholarly journals 1548. Association of bacterial colonization in ulcerative lesions of the diabetic foot together with the cicatrization process

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S773-S775
Author(s):  
Alexander A Ozoria Loveras ◽  
Jacques Paré Espinal ◽  
David de luna ◽  
Noemi Acevedo
Keyword(s):  
Diabetic Foot ◽  
Bacterial Colonization ◽  
Healing Process ◽  
Foot Ulcer ◽  
Primary Source ◽  
Diabetic Foot Ulcer ◽  
Care Program ◽  
Antimicrobial Treatment ◽  
Control Group ◽  
Experimental Group

Abstract Background In 2018, a review of the scientific literature identifed biofilm studies in the past 3 years. Wherein, the role of the biofilm in the progression of the diabetic foot ulcer with the presence of biofilm is reviewed. As a result, tissue sampling is more effective than transcure, Levin, and aspiration. A study in Cuba evaluate the effectiveness of a new proposal for antimicrobial treatment in patients with diabetic foot infection in 2017. The method used was a experimental type of two groups. The control group, was treated with the treatment scheme proposed in the Comprehensive Care Program for the patient with diabetic foot ulcer (PAIPUPD). Instead, the other experimental group was treated according to the scheme based on the local microbial map. They used a random distribution. Using the Wagner classification, there were patients classificate with a grade of W2 (13% of patients), W3 (66%) and W4 (12%). The most frequent germs found were Staphylococcus aureus, Escherichia coli and Enterobacter sp. The experimental group required less days with hospitalization. Methods This is an observational study of primary source with a non-probability sample with 58 patients. Adult patients diagnosed with type 2 diabetes mellitus, with diabetic foot, Wagner classification 1, 2 and 3, who attended the healing area of the Institute of Diabetes of Santiago were included. After identifying the colonized pathogen, its cicatrization process was evaluate for four weeks. Results Of the total sample, 12.07% belonged to Wagner 1, 43.1% to Wagner 2 and 44.83% to the Wagner 3 classification. In the case of the Wagner 3 group, S. aureus and Pseudomona aeruginosa were present in 6 cases. Despite the infectious processes detected by the culture, the patients showed a successful cicatrization process after four weeks of evaluation. Graph 1: Isolated Microorganism Vs Wagner’s Degree (n=60) Graph 2: Gram positive microorganism vs. healing process per week in patients with diabetic foot (n=22) Graph 3: Gram-negative microorganism vs. healing process (n=33) Conclusion During the four weeks of clinical evaluation, more than 88% of patients progressed positively to clinical recovery, regardless of the type of bacteria colonized and/or their resistance to antibiotics. Disclosures All Authors: No reported disclosures

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