scholarly journals Effect of Progesterone on Expression of MMP7 and MMP13 in Lungs of Female Mice

2018 ◽  
Author(s):  
Elahe Izadi ◽  
Fatemeh Vafashoar ◽  
Paria Jorbozedar ◽  
Mohamad Ali Assarehzadegan ◽  
Hadi Poormoghim ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Rna Extraction ◽  
Control Group ◽  
Quantitative Real Time Pcr ◽  
Specific Primers ◽  
New Era ◽  
Matrix Metalloproteinase 13 ◽  
Health And Disease ◽  
And Gender ◽  
The Impact

Gender medicine is a new era of science which focuses on the impact of sex hormones and gender on normal physiology, pathobiology and clinical features of diseases. In this study we investigated the impact of pregnancy doses of progesterone hormone on the expression of a couple of matrix metalloproteinase (MMPs), which are known to be involved in tissue remodeling of lungs in health and disease, namely MMP7 and 13.  Pregnancy maintenance dose of progesterone was administered to female BALB/c mice for 21 and 28 days, the control group received PBS for the same days. After removal of the lungs and RNA extraction, quantitative real-time PCR was done using specific primers for MMP7 and MMP13. We found that progesterone can slightly (not significantly) decrease the expression of MMP13 but had no effect on MMP7. Our results shows that progesterone has minimal effect on the expression of matrix metalloproteinase7 and matrix metalloproteinase 13, but it may still have an effect on corresponding tissue inhibitor of matrix metalloproteinases (TIMPs) or other components of the Extracellular matrix  which remains to be elucidated. Also, the effect of progesterone on these MMPs can be further studied in a fibrosis model.

Drug Induced Changes in Cell Organelles

1968 ◽  
Vol 26 ◽  
pp. 110-111
Author(s):  
Sarah A. Luse
Keyword(s):  
Clinical Medicine ◽  
Cell Organelles ◽  
Riboflavin Deficiency ◽  
Drug Induced ◽  
New Era ◽  
Health And Disease ◽  
In The Beginning ◽  
Cellular Pathology ◽  
Induced Changes ◽  
The Impact

In the mid-nineteenth century Virchow revolutionized pathology by introduction of the concept of “cellular pathology”. Today, a century later, this term has increasing significance in health and disease. We now are in the beginning of a new era in pathology, one which might well be termed “organelle pathology” or “subcellular pathology”. The impact of lysosomal diseases on clinical medicine exemplifies this role of pathology of organelles in elucidation of disease today.Another aspect of cell organelles of prime importance is their pathologic alteration by drugs, toxins, hormones and malnutrition. The sensitivity of cell organelles to minute alterations in their environment offers an accurate evaluation of the site of action of drugs in the study of both function and toxicity. Examples of mitochondrial lesions include the effect of DDD on the adrenal cortex, riboflavin deficiency on liver cells, elevated blood ammonia on the neuron and some 8-aminoquinolines on myocardium.

Detection of COX-2 in liquid biopsy of patients with prostate cancer

2021 ◽  
pp. jclinpath-2021-207755
Author(s):  
Vanessa Silva Pereira ◽  
Beatriz da Costa Aguiar Alves ◽  
Jaques Waisberg ◽  
Fernando Fonseca ◽  
Flavia Gehrke
Keyword(s):  
Prostate Cancer ◽  
Slow Growth ◽  
Rna Extraction ◽  
Specific Antigen ◽  
Common Type ◽  
Control Group ◽  
Quantitative Real Time Pcr ◽  
Diagnosis And Prognosis ◽  
The Mean ◽  
Cox 2

AimsTo determine the profile of COX-2 gene expression in patients with prostate cancer attended at the ABC University Health Center outpatient clinic and correlate the results with patients’ anatomopathological examinations. Prostate cancer is the sixth most common type of cancer worldwide and the second in Brazil. COX-2 expression is associated with an unfavourable prognosis.Methods15.0 mL of peripheral blood were collected from 24 patients and 25 healthy men. RNA extraction was performed using the QIAamp RNA Blood Mini Kit. Complementary DNA synthesis was performed using SuperScript II RNAse Reverse Transcriptase. Quantitative real-time PCR was performed with specific COX-2 oligonucleotides and the endogenous GAPDH gene.ResultsThe mean age of the patients was 69 years old. The Gleason scoring system showed 37.5% of patients with Gleason 6 (slow growth, low risk), 45.8% with Gleason 7 (intermediate risk) and 16.7% with Gleason 8 or 9 (risk of high-grade cancer). The median COX-2 expression in the study group was 0.97, while in the control group it was 0.11 (p<0.045).ConclusionsPatients with prostate cancer showed higher COX-2 expression at diagnosis compared with the control group. Since COX-2 detection associated with prostate-specific antigen dosage shows promise as a biomarker for diagnosis and prognosis in patients with prostate cancer, further research is required to confirm these findings.

scholarly journals Impact of Systematic Assessment of Instruction on Secondary School Students’ Physics Achievement at Cognitive Level of Knowledge

2021 ◽  
Vol 2 (1) ◽  
pp. 44-52
Author(s):  
Femi Adetunji Adeoye
Keyword(s):  
Secondary School ◽  
Cognitive Style ◽  
Analysis Of Covariance ◽  
Control Group ◽  
Physics Teaching ◽  
School Students ◽  
Systematic Assessment ◽  
Physics Concepts ◽  
And Gender ◽  
The Impact

The study examined empirically the impact of systematic and unsystematic assessment of instructions, cognitive style and gender on knowledge of physics concepts among Senior Secondary Two (SS II) Students in Lagos Island Local Government Area of Lagos State, Nigeria. It made use of 120 secondary school two (SS II) students (72 males and 48 females) in a pretest-posttest non-randomized control group design in which treatment systematic assessment at two levels was crossed with two levels of cognitive style and gender. Data analysis involved analysis of covariance and graphical illustration as post-hoc measures. The results indicate that allowing systematic attributes of continuous assessment (CASS) to come into play in physics teaching has significant effect on remembering of physics concepts across all the students’ cognitive style-gender groupings. The results also indicate a significant gender-group (in support of females) in remembering of physics concepts. These results show the need for secondary school physics teachers to bring in systematic characteristic of CASS into the assessment of physics teaching. The teachers should also be more patient with male students since their knowledge of physics concepts is significantly lower than that of their female counterparts.

scholarly journals Reduced Perceived Trustworthiness during Face Mask Wearing

2021 ◽  
Vol 11 (4) ◽  
pp. 1474-1484
Author(s):  
Giulio Gabrieli ◽  
Gianluca Esposito
Keyword(s):  
Impression Formation ◽  
Random Order ◽  
Face Mask ◽  
Control Group ◽  
Aesthetic Appearance ◽  
And Gender ◽  
The Aesthetic ◽  
Novel Coronavirus ◽  
The Impact ◽  
Experimental Group

To curb the diffusion of the novel coronavirus (SARS-CoV-2), governments worldwide have introduced different policies, including lockdowns, social distancing, and mandatory mask wearing. Face mask wearing, especially, has an impact on the formation of first impressions, given that when meeting someone for the first time, individuals rely on the only available piece of information, the newly met person’s aesthetic appearance, in order to make initial estimations of other traits, such as competence, intelligence, or trustworthiness. However, face mask wearing affects the aesthetic appearance of an individual, creating uncertainty which, in turn, has been reported to reduce others’ perceived trustworthiness. In this paper, the influence of face mask wearing on strangers’ perceived trustworthiness and aesthetic appearance is assessed to verify the impact of this policy on impression formation. Participants (N = 71) have been instructed to assess the trustworthiness and the aesthetic appearance of a selection of 96 images depicting individuals of different ages (children, adults, and older adults), gender (men and women), and ethnicity (Asians or Caucasians). Participants were randomly divided into two groups: an experimental group and a control group. Participants in the experimental group (N = 38) rated faces of individuals wearing a face mask, while participants in the control group rated the same faces but in the absence of a face mask. Images were presented in random order. For each face, participants were asked to rate the aesthetic appearance and perceived trustworthiness of the stranger on two different 100-point Likert scales. Results demonstrate that (i) the correlation between perceived trustworthiness and aesthetic appearance is not affected by the presence of a face mask, and (ii) age, but not ethnicity and gender, influences the magnitude of differences in perceived trustworthiness levels during mask wearing.

Decision Making after Traumatic Brain Injury: A Temporal Discounting Paradigm

2013 ◽  
Vol 19 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Rodger Ll. Wood ◽  
Louise McHugh
Keyword(s):  
Traumatic Brain Injury ◽  
Decision Making ◽  
Executive Function ◽  
Brain Injury ◽  
Temporal Discounting ◽  
Monetary Reward ◽  
Control Group ◽  
Neuropsychological Measures ◽  
And Gender ◽  
The Impact

AbstractA temporal discounting paradigm was used to examine decision making for hypothetical monetary reward following traumatic brain injury (TBI). A case-control design compared individuals following moderate or severe TBI with a healthy control group matched for age and gender. The impact of intelligence, impulsivity, and mood on temporal discounting performance was examined. A within-subjects design for the TBI group determined the influence of a range of neuropsychological tests on temporal discounting performance. Both patients and controls demonstrated temporal discounting. However, the TBI group discounted more than controls, suggesting that their decision making was more impulsive, consistent with ratings on the impulsiveness questionnaire. Discounting performance was independent of neuropsychological measures of intelligence, memory, and executive function. There was no relationship between temporal discounting and ratings of everyday executive function made by patients' relatives. Low mood did not account for discounting performance. The results of this study suggest that temporal discounting may be a useful neuropsychological paradigm to assess decision making linked to monetary reward following TBI. Performance was relatively independent of intelligence, memory and standard tests of executive ability and may therefore assist when assessing a patient's mental capacity to manage their financial affairs. (JINS, 2013,19, 1–8)

scholarly journals Diseases and Geographic Variation: Are we missing the whole picture?

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Gareth John
Keyword(s):  
Large Scale ◽  
Single Point ◽  
Control Group ◽  
Epidemiological Analysis ◽  
Control Approach ◽  
Time Period ◽  
Risk Approach ◽  
History Of ◽  
Health And Disease ◽  
And Gender

ABSTRACT BackgroundEpidemiology is the study and analysis of the patterns, causes, and effects of health and disease conditions in defined populations. However, where an epidemiological analysis relies solely on data from large-scale anonymous administrative data sources, the available geographic information for the individuals concerned relates in general to just one single point in time; for example the places of residence of individual on the day that they were registered with a particular disease, or their places of residence on the day that they were admitted to hospital. This information may not be sufficient however, especially when considering diseases where there may be a long period of time between an exposure to a particular hazard and the subsequent onset of disease. MethodA solution to this problem possibly lies within administrative sources of data such as the Welsh Demographic Service (WDS), which is accessible to NHS Wales analysts and users of the SAIL databank in a pseudonymised format. The WDS contains the details of all Welsh residents who have been registered with a GP Practice since 1992, including a full history of changes to their addresses and GP practices. This data can be used to easily ascertain an individual’s address at any point in time, for example on a particular census date, or within a time period that is relative to a particular event, e.g. 10 years prior to disease registration. However, this research will look at how to incorporate all of an individual’s available address information into an epidemiological analysis. ResultsTwo main approaches will be demonstrated; the first using a “Person Years at Risk” approach which attempts to apportion numerator and denominator according to the number of previous residences, and the second using a Case Control approach, comparing the geographic spread of addresses in the diseased group of patients versus the non-diseased (control) group, with age and gender matched controls also drawn from the WDS.

scholarly journals Pengujian Fenomena Underpricing Dengan Studi Eksperimen

2010 ◽  
Vol 3 (1) ◽  
Author(s):  
Dedhy Sulistiawan
Keyword(s):  
Decision Making ◽  
Treatment Group ◽  
Conflict Of Interest ◽  
Economic Benefit ◽  
Control Variable ◽  
Control Group ◽  
Financial Benefit ◽  
And Gender ◽  
And Control ◽  
The Impact

The purpose of this study is to examine the impact of conflict of interest to the level of IPO price determined by managers. This experiment divides treatment group (the group of participants who have economic benefit from their decision making), and control group (groups with no economic benefit from their decision making). In experiment scenario for treatment group, the managements of IPO companies tend to decrease the price of IPO in order to get financial benefit. Using experimental design, this research shows that participants in treatment group determine lower IPO price than control group. This evidence confirms IPO phenomenon around the world. This research also makes supplementary analysis. The results show that alternative statistical test using GPA and gender of participants as control variable are not statistically significant. It means that the level of IPO price isn’t determined by GPA and Gender, but conflict of interest.

scholarly journals Pre-Clinical Study: Immunohistochemical evaluation of matrix metalloproteinase-13 on rabbit (Oryctolagus cuniculus) socket healing after application of platelet-rich fibrin with and without hydroxyapatite

F1000Research ◽  
2022 ◽  
Vol 11 ◽  
pp. 29
Author(s):  
META MAULIDA DAMAYANTI ◽  
MEIKE RACHMAWATI
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinase ◽  
Oryctolagus Cuniculus ◽  
Healing Process ◽  
Observation Time ◽  
Control Group ◽  
Wound Healing Process ◽  
Platelet Rich Fibrin ◽  
Matrix Metalloproteinase 13 ◽  
Socket Healing

Background: Tissue engineering technology has been used globally and proven to accelerate wound healing. This study aimed to analyse the effect of adding hydroxyapatite (HA) as a scaffold to platelet-rich fibrin (PRF) as a growth factor in accelerating the wound healing process as seen from the expression of matrix metalloproteinase-13 (MMP-13). Methods: This research is an animal experiment conducted on 18 rabbits (Oryctolagus cuniculus). Rabbits were randomly divided into the following three groups of treatment: (G1) the application of PRF group, (G2) the application of PRF+HA group and (C) the control group without any application. Furthermore, each treatment group was split randomly into three groups of observation time. Periodontal tissue biopsy was performed to analyse the histopathological features that were examined on the basis of the level of MMP-13 immunoexpression. Results: MMP-13 immunoexpression in the PRF+HA group showed better histoscore results, indicating a substantial reduction in MMP-13 values compared with other groups. The healing process was shown to increase with increasing observation time (p<0.05), and the PRF+HA group outperformed the PRF and control groups. On day 3, MMP-13 exhibited a dark brown colour of Immunohistochemistry (IHC), which indicated an increase in the expression value of MMP-13 in the early stages of healing, namely, inflammation. On day 14, light brown IHC was seen, especially in group 2, as a reference that the remodeling process had begun. Conclusions: This study indicates that the application of HA can accelerate the socket healing process by decreasing the level of immunoexpression of MMP-13. HA is an alloplastic material that has inherent bioactive properties that support osteoconduction, which functions as a scaffold in the form of a fibrin matrix that can bind MMPs so that it can accelerate the wound healing process.

scholarly journals The Impact of an International Component in Educating the Public Using an Experimental Design

2019 ◽  
pp. 102831531988989
Author(s):  
Uwe Brandenburg ◽  
Bryn Willcock
Keyword(s):  
Experimental Design ◽  
Intervention Group ◽  
Group Session ◽  
Control Group ◽  
Random Group ◽  
The Public ◽  
Group Assignment ◽  
The United Kingdom ◽  
And Gender ◽  
The Impact

This study focuses on the effect of internationalization on the wider society. The study analyses the results of a session on terrorism and media, where participants were the wider public in the United Kingdom. An experimental design with random group assignment was conducted where internationalization was operationalized by the training either being international, where a U.S. trainer co-conducted the intervention group session, or national, where a U.K. trainer co-conducted the control group session. Both groups were matched for age, education, and gender. While both groups did not significantly differ regarding a core personality trait—that is, openness—as well as the learning outcomes prior to the training, they differed significantly post training. Training on terrorism showed consistent learning effects for both groups, but was far greater in the presence of the U.S. co-teacher and these effects were long-term. Consistent with predictions, the presence of an international trainer produced significantly larger effects when compared with when the trainer was not international.

scholarly journals PTSD in parents of children with severe diseases: a systematic review to face Covid-19 impact

2021 ◽  
Vol 47 (1) ◽  
Author(s):  
Martina Corsi ◽  
Alessandro Orsini ◽  
Virginia Pedrinelli ◽  
Andrea Santangelo ◽  
Carlo Antonio Bertelloni ◽  
...  
Keyword(s):  
Traumatic Stress ◽  
Data Extraction ◽  
Control Group ◽  
Post Traumatic Stress ◽  
Study Selection ◽  
Pubmed Database ◽  
Methodological Errors ◽  
Post Traumatic ◽  
And Gender ◽  
The Impact

Abstract Context The literature agrees on the impact of post-traumatic stress symptoms in parents of seriously ill children but there is less clarity about the real extent and gender differences of this psychopathological risk. The recent Covid-19 outbreak highlighted new burdens for researchers on Post Traumatic Stress Disorder (PTSD) and clear evidence-based knowledge on this issue is timely needed. Objective In this review, we present a synthesis of the updated evidence on PTSD rates in parents of children with severe diseases. We also aim to try to understand if research in this field has been refined over time with the long-term intent to better face the new challenges of Covid-19 in the paediatric field. Data sources The PubMed database was searched. Study selection Studies were included if they assessed PTSD in parents of children diagnosed with physical illnesses. Data extraction Of 240 studies, 4 were included. Results Analysis of the 4 studies revealed 2 studies with PTSD rates around 20% and in line with previous best-evidence. All 4 studies tried to provide more data on fathers, however, all the studies present the lack of a control group. Limitations The limited number of studies, which also differ widely in the methodology used. Conclusions Methodological errors evidenced in all the 4 studies limit their reliability, making the understanding of the paediatric caregiver’s concern regarding PTSD still difficult. More sound research is needed.

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