The Levels of Serum Biomarkers in Stable Asthma Patients with Comorbidities

The effects of comorbidities on systemic inflammation markers in stable asthmatics and the consequences of such effects have not been well evaluated. We aimed to evaluate the effect of comorbidities on clinical manifestations and systemic inflammation in asthmatic patients under control. The study group consisted of asthmatic patients who applied to our pulmonology outpatient clinic and volunteered to participate. 120 clinically stable asthma patients (71 females and 49 males) and 35 healthy controls (19 females and 16 males) with similar age, gender, and body mass index distributions were admitted to the study. The levels of osteopontin, interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 13 (IL-13), eosinophilic cationic protein, adiponectin, and high-sensitivity C-reactive protein of the individuals were evaluated using commercial ELISA kits by taking venous blood samples. Of 120 asthmatic subjects, 47 (39,2%) had comorbidities and allergic rhinitis (15%) coexisted most frequently. Other comorbidities associated with asthma were gastroesophageal reflux, sinusitis, hypertension, diabetes, gastritis, and peptic ulcus respectively. There was no physician-diagnosed comorbidity in the control group. The levels of IL-6 and IL-8 were found higher in asthma group with comorbidities when compared to those with no comorbidities (p values were 0.032 and 0.046, respectively). Comorbidities interfere with the diagnosis and treatment of asthma, besides affecting the disease control. Our findings suggest the possibility of the impact of comorbidities on systemic inflammation markers, especially IL-6 and IL-8. To evaluate the impact of comorbidities on asthma control and systemic markers, further studies are needed.

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Weight reduction improves immune system and inflammatory cytokines in obese asthmatic patients

African Health Sciences ◽

10.4314/ahs.v20i2.44 ◽

2020 ◽

Vol 20 (2) ◽

pp. 897-902

Author(s):

Fadwah M Al-Sharif ◽

Shehab M Abd El-Kader ◽

Ziyad A Neamatallah ◽

Afnan M AlKhateeb

Keyword(s):

Immune System ◽

Systemic Inflammation ◽

Weight Reduction ◽

Inflammation Markers ◽

Mean Values ◽

Asthmatic Patients ◽

Asthma Patients ◽

Group A ◽

The Mean ◽

Group B

Background: Activation of immunological and systemic inflammation markers are common in obesity and asthma. Objective: The target of this study was to assess impact of weight reduction on immunological and systemic inflammation markers in obese asthma patients. Material and methods: Eighty asthmatic patients of both sex; their age and body mass index (BMI) mean were 38.72 ± 7.14 year and 32.65 ± 3.18 Kg/m2 respectively. Exclusion criteria included smokers, infections, vaccinations, cancer, surgery, immune system disorders and medications that may influence immune system function as anti-inflammatory medications, analgesics and anti-depressant. All subjects were randomly enrolled in weight reduction group (group A) or control group (group B). Results: The main findings in the present study indicated that weight reducing program in group (A) was associated with significant reduction in the mean values of IL6, TNF-α, and IL8 in addition to significant increase in the mean values of CD4 and CD8 cell count . However, findings of group (B) showed no significant changes. Moreover, Comparison between both groups at the end of the study revealed significant differences. Conclusion: Weight reduction improved immunological and systemic inflammation markers in obese asthma patients. Keywords: Bronchial asthma; cytokines; obesity; immune system; weight reduction.

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Effect of Whole-Body Cryotherapy on Morphological, Rheological and Biochemical Indices of Blood in People with Multiple Sclerosis

Journal of Clinical Medicine ◽

10.3390/jcm10132833 ◽

2021 ◽

Vol 10 (13) ◽

pp. 2833

Author(s):

Bartłomiej Ptaszek ◽

Aneta Teległów ◽

Justyna Adamiak ◽

Jacek Głodzik ◽

Szymon Podsiadło ◽

...

Keyword(s):

Multiple Sclerosis ◽

Neurological Diseases ◽

Venous Blood ◽

Whole Body ◽

Controlled Study ◽

Control Group ◽

Blood Indices ◽

Rheological Indices ◽

Whole Body Cryotherapy ◽

The Impact

The aim of this study was to examine and assess the impact of a series of 20 whole-body cryotherapy (WBC) treatments on the biochemical and rheological indices of blood in people with multiple sclerosis. In this prospective controlled study, the experimental group consisted of 15 women aged 34–55 (mean age, 41.53 ± 6.98 years) with diagnosed multiple sclerosis who underwent a series of whole-body cryotherapy treatments. The first control group consisted of 20 women with diagnosed multiple sclerosis. This group had no intervention in the form of whole-body cryotherapy. The second control group consisted of 15 women aged 30–49 years (mean age, 38.47 ± 6.0 years) without neurological diseases and other chronic diseases who also underwent the whole-body cryotherapy treatment. For the analysis of the blood indices, venous blood was taken twice (first, on the day of initiation of whole-body cryotherapy treatments and, second, after a series of 20 cryotherapy treatments). The blood counts were determined using an ABX MICROS 60 hematological analyzer (USA). The LORCA analyzer (Laser–Optical Rotational Cell Analyzer, RR Mechatronics, the Netherlands) was used to study the aggregation and deformability of erythrocytes. The total protein serum measurement was performed using a Cobas 6000 analyzer, Roche and a Proteinogram-Minicap Sebia analyzer. Fibrinogen determinations were made using a Bio-Ksel, Chrom-7 camera. Statistically significant differences and changes after WBC in the levels of red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), elongation index, total extend of aggregation (AMP), and proteins (including fibrinogen) were observed. However, there was no significant effect of a series of 20 WBC treatments on changes in blood counts, rheology, and biochemistry in women with multiple sclerosis. Our results show that the use of WBC has a positive effect on the rheological properties of the blood of healthy women.

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The Utility of Serum IL-1β and CRP Together with Fractional Exhaled Nitric Oxide in the Diagnosis of Asthma in Adults

NeuroQuantology ◽

10.14704/nq.2021.19.8.nq21122 ◽

2021 ◽

Vol 19 (8) ◽

pp. 119-124

Author(s):

Hayder Abdul-Amir Makki Al-Hindy ◽

Ali Jihad Hemid Al-Athari ◽

Mazin J. Mousa ◽

Safa Jihad Hameed ◽

Suhad Hafidh Obeed

Keyword(s):

Nitric Oxide ◽

Sensitivity And Specificity ◽

Bronchial Asthma ◽

High Sensitivity ◽

Exhaled Nitric Oxide ◽

Fractional Exhaled Nitric Oxide ◽

Asthmatic Patients ◽

Significant Difference ◽

Asthma Patients ◽

Hs Crp

Background: Bronchial asthma (BrA), recognized lately as an umbrella, covers various subtypes rather than only one disease. Asthma is a chronic inflammation of the airways, in which cytokines could play a crucial role in its pathogenesis. Hence, labors to progress noninvasive markers for asthma had centered through this era. Presently, the fractional exhaled nitric oxide (FeNO), serum C-reactive protein (CRP), and interleukin levels are emerging analytical biomarkers in this field. FeNO is a noninvasive and practical tool even in mild asthma. This study aimed to evaluate the utility of serum IL-1β and CRP together with fractional exhaled nitric oxide in the diagnosis of adult bronchial asthma. Method: The study was a case control, including 150-patients and 100-healthy controls. FeNO tests, measurements of plasma levels IL-1β and HS-CRP had undertaken for all the participants. The statistical data had examined by SPSS (V/27) for Windows. Descriptive data of the variables had compatibly used. A significance lower than or identical to 0.05 had intended. ROC curve examination of FeNO tests, IL-1β, and HS-CRP, to predict asthma from healthy control had applied. Results: there was a significant difference in the FeNo test, HS-CRP levels, and BMI, while no significant difference in all other variables between the groups. The FeNo results correlate positively, though not significantly, with the levels of IL-1β in asthmatic patients (> 0.05). There was a nonsignificant negative correlation between the FeNo results with the level of HSCRP. The accuracy, sensitivity, and specificity of the IL-1β to distinguish asthma were 68.6% and 58% at 95% CI [0.41-0.745], respectively, which was not significant (p>0.05). However, ROC analysis of HS-CRP revealed predictability for asthma patients (p-0.000), with higher accuracy, sensitivity, and specificity: 89.9%, and 68.1% at 95% CI [0.820-0.979], respectively. The FeNo tests revealed highly significant (0.000), high sensitivity, and specific (91% for both) with high 95% CI [0.938-1.000] predictability for asthma. Conclusion: The utility of circulating HS-CRP is more valuable than IL-1β when combined with fractional exhaled nitric oxide in the diagnosis of asthma. Novel biomarkers could improve the precision of this field.

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Lung function decline in asthma patients with elevated bronchial CD8, CD4 and CD3 cells

European Respiratory Journal ◽

10.1183/13993003.01525-2015 ◽

2016 ◽

Vol 48 (2) ◽

pp. 393-402 ◽

Cited By ~ 22

Author(s):

Irene den Otter ◽

Luuk N.A. Willems ◽

Annemarie van Schadewijk ◽

Simone van Wijngaarden ◽

Kirsten Janssen ◽

...

Keyword(s):

Lung Function ◽

Granzyme B ◽

Eosinophil Cationic Protein ◽

Risk Groups ◽

Forced Expiratory Volume ◽

Lung Function Decline ◽

Cationic Protein ◽

Asthmatic Patients ◽

Asthma Patients

Which inflammatory markers in the bronchial mucosa of asthma patients are associated with decline of lung function during 14 years of prospective follow-up?To address this question, 19 mild-to-moderate, atopic asthmatic patients underwent spirometry and bronchoscopy at baseline and after 14 years of follow-up (t=14). Baseline bronchial biopsies were analysed for reticular layer thickness, eosinophil cationic protein (EG2), mast cell tryptase (AA1), CD3, CD4 and CD8. Follow-up biopsies were stained for EG2, AA1, neutrophil elastase, CD3, CD4, CD8, CD20, granzyme B, CD68, DC-SIGN, Ki67 and mucins.Decline in forced expiratory volume in 1 s (FEV1) % predicted was highest in patients with high CD8 (p=0.01, both pre- and post-bronchodilator) or high CD4 counts at baseline (p=0.04 pre-bronchodilator, p=0.03 post-bronchodilator). Patients with high CD8, CD3 or granzyme B counts at t=14 also exhibited faster decline in FEV1 (p=0.00 CD8 pre-bronchodilator, p=0.04 CD8 post-bronchodilator, p=0.01 granzyme B pre-bronchodilator, and p<0.01 CD3 pre-bronchodilator).Long-term lung function decline in asthma is associated with elevation of bronchial CD8 and CD4 at baseline, and CD8, CD3 and granzyme B at follow-up. This suggests that high-risk groups can be identified on the basis of inflammatory phenotypes.

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Investigation on the Correlation between Serum Immune Factor Levels and Allergic Constitution in Children with Infectious Mononucleosis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/7368642 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Hong Sun ◽

Weiqun Wang ◽

Chenglei Lin ◽

Min Chen

Keyword(s):

Infectious Mononucleosis ◽

Clinical Symptoms ◽

Venous Blood ◽

Clinical Manifestations ◽

Allergic Diseases ◽

Early Morning ◽

Control Group ◽

Serum Ige ◽

Immune Factor ◽

Reference Index

Objective. To investigate the correlation between serum immune factor levels and allergic constitution in children with infectious mononucleosis. Methods. A total of 120 children who visited our hospital from March, 2019, to December, 2020, were selected as the research objects, and 40 children who came to our hospital for physical examination were included in the control group (CG). 40 children with IM were classified into the IM group (IG), and 40 IM children with allergic rhinitis, allergic dermatitis, asthma, and other allergic diseases were classified into the IM allergy group (AG). On the second day of admission, 5 ml of fasting venous blood was collected from all children in the early morning to observe the serum IgE level, the level of lymphocyte subsets, and the level of immunoglobulin of the patient. Results. The serum CD3, CD4, and CD8 levels of children in AG were significantly higher than those in IG and CG ( P < 0.05 ). The serum IgE, IgA, IgM, and IgG levels of children in AG were significantly higher than those of IG and CG ( P < 0.05 ). The serum IgE levels of children in AG were positively correlated with the serum CD3, CD4, and CD8 levels ( P < 0.05 ). There was a positive correlation between the serum IgE level and serum IgA, IgM, and IgG levels in children with AG ( P < 0.05 ). Conclusion. The results of this study showed that there may be a certain relationship between allergic constitution and the incidence, clinical manifestations, and prognosis of infectious mononucleosis. IgE level can be used as a reference index for the early severity of IM clinical symptoms.

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THE ROLE OF INFLAMMATION AND COAGULATION CASCADE IN THE PATHOGENESIS OF ATRIAL FIBRILLATION

ASJ. ◽

10.31618/asj.2707-9864.2020.3.41.32 ◽

2020 ◽

Vol 3 (41) ◽

pp. 8-10

Author(s):

L. Hazarapetyan ◽

S. Grigoryan ◽

A. Sarksyan

Keyword(s):

Atrial Fibrillation ◽

Coagulation Cascade ◽

Control Group ◽

Atrial Remodeling ◽

Inflammation Markers ◽

Reactive Protein ◽

Gender And Age ◽

The Impact ◽

The Relationship

Introduction: Atrial fibrillation (AF) is associated with prothrombotic or hypercoagulable states, various inflammation markers such as interleukin-6 (IL-6) and hsC-reactive protein (hsCRP) have also been associated with AF. The aim of this study is to investigate the relationship between inflammation markers and the prothrombotic state in the setting of AF and the impact on outcome in patients with AF. Methods: We observed 141 patients with non-valvular AF. As a control group patients similar in gender and age without AF were examined. Clinical, instrumental and laboratory tests were performed on the observed patients. The markers of the coagulation cascade (TF and F) and of inflammatory markers (hsCRP and IL-6) were studied additionally by ELISA on the analyzer "Stat Fax 303 Plus". Studies were conducted using SPSS 13.0 and EXCEL-2013. Results: The obtained results showed that compared to the control group, AF patients had significantly higher levels of IL-6 (p = 0.043), hsCRP (p = 0.002), TF (p = 0.026), and F (p = 0.025). Moreover, levels of hsCRP were higher among AF patients at "high" risk of stroke by CHA2DS2-VASc Score (p = 0.003). Besides, the levels of hsCRP and IL-6 were markedly elevated in patients with dilated left atrium (p = 0.001), poorly functioning left atrial appendage (p = 0.023) and longer duration of AF (p = 0.002). Conclusion: We have demonstrated that the increased plasma levels of IL-6 and hsCRP are related to indices of the coagulation cascade and contribute to structural atrial remodeling in patients with AF.

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Assessment of serum uric acid levels in patients with cervical intraepithelial neoplasia in Benin City

Annals of Clinical and Biomedical Research ◽

10.4081/acbr.2021.99 ◽

2021 ◽

Vol 2 (1) ◽

Author(s):

Kenneth Atoe ◽

Augustine Onovuakpo Eguvbe

Keyword(s):

Uric Acid ◽

Cervical Intraepithelial Neoplasia ◽

Serum Uric Acid ◽

Venous Blood ◽

High Sensitivity ◽

Intraepithelial Neoplasia ◽

Control Group ◽

Benin City ◽

Malignant Lesions ◽

Edo State

Elevated Uric acid levels in humans have been associated with cancer, though the pathogenic mechanism is still unclear. The present study attempts to investigate if elevated uric acid levels could be used to investigate the pre-malignant lesions leading to cervical cancer. The study which was carried out at the Department of Obstetrics and Gynaecology, University of Benin Teaching Hospital Benin City and Department of Chemical Pathology, Edo University Iyamho, Edo state Nigeria, between August, 2017 and January 2019, examined the capacity of detecting Cervical Intraepithelial Neoplasia (CIN) early with serum uric acid in order to increase the chances of survival of women at risk. A total of 197 female participants were recruited for the study. Based on histological diagnosis, they were grouped as CIN 1, CIN 2 and CIN 3. The control group were negative for CIN. Venous blood was obtained from participants and serum uric acid levels were determined using standard laboratory methods. The benchmark for serum uric acid levels in diagnosing CIN presented in this study was at 6.55 mg/dL. The probability that uric acid level higher than 6.55 mg/dL indicate the disease probabilty was 93.1%. The age category within the CIN group with the highest level of uric acid was 31-40 years (10.86 mg/dL). The use of serum uric acid as an excellent biomarker for the diagnosis of CIN is therefore suggested due to its high sensitivity.

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Intradialytic cycling does not exacerbate microparticles or circulating markers of systemic inflammation in haemodialysis patients

European Journal of Applied Physiology ◽

10.1007/s00421-021-04846-7 ◽

2021 ◽

Author(s):

Patrick J. Highton ◽

Daniel S. March ◽

Darren R. Churchward ◽

Charlotte E. Grantham ◽

Hannah M. L. Young ◽

...

Keyword(s):

Systemic Inflammation ◽

Perceived Exertion ◽

Venous Blood ◽

Rating Of Perceived Exertion ◽

Moderate Intensity ◽

Inflammatory Status ◽

Intensity Rating ◽

Circulating Markers ◽

Post Hoc ◽

The Impact

Abstract Purpose Patients receiving haemodialysis (HD) display elevated circulating microparticle (MP) concentration, tissue factor (TF) expression and markers of systemic inflammation, though regular intradialytic cycling (IDC) may have a therapeutic effect. This study investigated the impact of regular, moderate-intensity IDC on circulating MPs and inflammatory markers in unit-based HD patients. Methods Patients were cluster-randomised to intervention (n = 20, age: 51.4 ± 18.1 years, body mass: 77.6 ± 18.3 kg, mean ± SD) or no-exercise control (n = 20, 56.8 ± 14.0 years, 80.5 ± 26.5 kg). Intervention participants completed 30 min of moderate intensity (rating of perceived exertion [RPE] of 12–14) IDC, thrice weekly for 6 months. Pre-dialysis venous blood samples were obtained at 0, 3 and 6 months. Circulating MP phenotypes, cytokines, chemokine and MP TF expression were quantified using flow cytometry and cytometric bead array assays. Results Despite high exercise compliance (82%), no IDC-dependent effects were observed for any MP, cytokine or chemokine measure (p ≥ 0.051, ηρ2 ≤ 0.399) other than TNF-α (p = 0.001, ηρ2 = 0.186), though no significance was revealed upon post hoc analysis. Conclusion Six months of regular, moderate-intensity IDC had no effect on MPs, cytokines or chemokines. This suggests that the exercise did not exacerbate thrombotic or inflammatory status, though further functional assays are required to confirm this. Trial registration ISRCTN1129707, prospectively registered on 05/03/2015.

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PROGNOSTIC INDICES AND MARKERS OF SYSTEMIC INFLAMMATION IN PATIENTS WITH COPD AND CAD

Medical Council ◽

10.21518/2079-701x-2017-4-81-85 ◽

2017 ◽

pp. 81-85

Author(s):

G. L. IGNATOVA ◽

V. N. ANTONOV

Keyword(s):

Systemic Inflammation ◽

Prognostic Markers ◽

Functional Disorders ◽

Inflammation Markers ◽

C Reactive Protein ◽

Reactive Protein ◽

Male Patients ◽

The Impact ◽

The Relationship

The article discusses the problem of COPD combined with CAD, determination of inflammatory markers and the prognostic BODE, DOSE and ADO indices. The aim of the study was to evaluate the effect of PCV13 immunization on clinical and functional manifestations of systemic inflammation in patients with COPD and CAD, as well as the relationship with the prognostic markers. Material and methods: The study included 36 male patients with COPD and 36 patients with COPD and CAD, FC II-III stable angina who were treated at Regional Clinical Hospital _4 in Chelyabinsk during the period 2015--2016. Conclusions: 1. Patients with COPD combined with CAD had statistically significant clinical and functional disorders manifested by an increase in the degree of dyspnea and reduction in FEV1. 2. In patients with COPD concomitant with CAD, against the background of the comorbidities, the levels of systemic inflammation markers — C-reactive protein, fibrinogen and procalcitonin — were more overt than in patients only with COPD. 3. Monitoring changes in procalcitonin levels could be used as an additional highly informative method for estimating the probability of the presence of bacterial inflammation and effectiveness of therapy. 4. The prognostic BODE, DOSE and ADO indices tend to decrease unidirectionally under the impact of vaccination with pneumococcal conjugate vaccine. 5. PCV13 can reduce the level of systemic inflammation within 1 year after its application.

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Small RNA Species and microRNA Profiles are Altered in Severe Asthma Nanovesicles from Broncho Alveolar Lavage and Associate with Impaired Lung Function and Inflammation

Non-Coding RNA ◽

10.3390/ncrna5040051 ◽

2019 ◽

Vol 5 (4) ◽

pp. 51 ◽

Cited By ~ 3

Author(s):

Ana S. Francisco-Garcia ◽

Eva M. Garrido-Martín ◽

Hitasha Rupani ◽

Laurie C. K. Lau ◽

Rocio T. Martinez-Nunez ◽

...

Keyword(s):

Severe Asthma ◽

Small Rna ◽

Small Rna Sequencing ◽

Filtration Method ◽

Asthmatic Patients ◽

Expression Of Genes ◽

Severe Asthmatic ◽

Asthma Patients ◽

Impaired Lung Function ◽

The Impact

MicroRNAs are known to regulate important pathways in asthma pathology including the IL-6 and IFN pathways. MicroRNAs have been found not only within cells but also within extracellular vesicles such as exosomes. In this study, we particularly focused on microRNA cargo of nanovesicles in bronchoalveolar lavage of severe asthmatic patients. We extracted nanovesicle RNA using a serial filtration method. RNA content was analyzed with small RNA sequencing and mapped to pathways affected using WebGestalt 2017 Software. We report that severe asthma patients have deficient loading of microRNAs into their airway luminal nanovesicles and an altered profile of small RNA nanovesicle content (i.e., ribosomal RNA and broken transcripts, etc.). This decrease in microRNA cargo is predicted to increase the expression of genes by promoting inflammation and remodeling. Consistently, a network of microRNAs was associated with decreased FEV1 and increased eosinophilic and neutrophilic inflammation in severe asthma. MicroRNAs in airway nanovesicles may, thus, be valid biomarkers to define abnormal biological disease processes in severe asthma and monitor the impact of interventional therapies.

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