Identification of pathogenic bacteria from food handling surfaces (tabletops) from different areas with demonstration of their drug resistance properties

2021 ◽  
Author(s):  
Anik Paul ◽  
Md. Mahmud Rahma ◽  
Tasnia Ahmed
Keyword(s):  
Fast Food ◽  
Pathogenic Bacteria ◽  
Klebsiella Oxytoca ◽  
Food Contamination ◽  
Antibiotic Sensitivity ◽  
Klebsiella Pneumonia ◽  
Microbiological Analysis ◽  
Food Handling ◽  
Salmonella Spp ◽  
Esherichia Coli

Foodborne illness is generally caused after consumption of food contaminated with pathogenic microorganisms. Food contamination often caused by contact with tabletops or food handling surfaces where the pathogenic microbes are present due to unhygienic condition of people working there and the overall environment of the food serving area. In current study, four areas (local restaurants, fast food shops, university canteens and hospital canteens) were selected for collection of swab sample (per cm2 area) from the tabletops. Five samples from each area were taken for further studies. After microbiological analysis we found ten different types of bacteria (Esherichia coli, Klebsiella pneumonia, Klebsiella oxytoca, Corynebacterium xerosis, Staphylococcus aures, Salmonella spp., Proteus mirabilis, Enterobacter aerogenes, pseudomonas aeruginosa and Alcaligenes fecalis) which are already considered to be pathogenic bacteria causing different health issues in immune-compromised and also in healthy consumers. These bacteria were then subjected to antibiotic sensitivity test using ten antibiotics-Vancomycin (30 µg), Cotrimoxazol (30 µg), Azithromycin (15 µg), Gentamicin (10 µg), Amoxycillin (10 µg), Cephradine (30 µg), Ceftriaxone (30 µg), Cefuroxime (30 µg), Cefoxitin (30 µg) and Tetracycline (30 µg). Bacterial isolates collected from university and hospital canteens showed most resistance towards these antibiotics. Strict maintenance of proper sanitation and hygiene starting from personal aspects to the overall environment of food handling service should be maintained to reduce the food contamination and foodborne disease.

Comparing antibiotic resistance in commensal and pathogenic bacteria isolated from wild-caught South Carolina shrimps vs. farm-raised imported shrimps

2007 ◽  
Vol 53 (7) ◽  
pp. 919-924 ◽  
Author(s):  
Kavitha Boinapally ◽  
Xiuping Jiang
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
South Carolina ◽  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Vibrio Vulnificus ◽  
Resistant Bacteria ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Plate Counts

The objective of this study was to assess and differentiate wild-caught South Carolina (SC) shrimps from imported shrimps on the basis of microbiological analysis. Seven wild-caught SC shrimp and 13 farm-raised imported shrimp samples were analyzed. Total plate counts from wild-caught shrimp samples ranged from 4.3 to 7.0 log10 CFU/g, whereas counts from imported shrimp samples ranged from 3.2 to 5.7 log10 CFU/g. There was no difference (P > 0.05) between total bacterial counts of wild-caught SC shrimp and farm-raised imported shrimp. However, the percentages of bacteria with reduced susceptibility towards ceftriaxone and tetracycline were higher (P < 0.05) for farm-raised shrimp than for wild-caught samples. Salmonella spp. detected only in one farm-raised sample was resistant to ampicillin, ceftriaxone, gentamicin, streptomycin, and trimethoprim. Vibrio vulnificus was detected in both wild-caught and farm-raised shrimp samples; however, only the isolate from farm-raised shrimp was resistant to nalidixic acid and trimethoprim. Escherichia coli detected in one wild-caught sample was resistant to ampicillin. Both Listeria spp. and Salmonella spp. were absent with wild-caught SC samples. Therefore, the presence of more ceftriaxone- and tetracycline-resistant bacteria and the observed antimicrobial resistance phenotypes of isolates from the imported shrimp may reflect the possible use of antibiotics in raising shrimp in those countries.

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Prevalence of Bacterial Vaginosis among Married Women in Kalar District, Iraqi Kurdistan Region

passer ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 194-199
Author(s):  
Saman Mohammed Mohammed- Amin
Keyword(s):  
Bacterial Vaginosis ◽  
Pathogenic Bacteria ◽  
Clinical Symptoms ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Married Women ◽  
P Value ◽  
Klebsiella Pneumonia ◽  
Microbiological Analysis ◽  
Isolation And Identification

Abstract Bacterial vaginosis (BV) is an inflammatory disease, caused by polymicrobial infection, including pathogenic bacteria which replace the vaginal normal flora and finally this replacement causes manifestations of several physiological and clinical symptoms among women within different ages. BV has become one of the main problems that make woman patients visit gynecological and obstetric consultant hospitals in most country. The present study is designed to determine the causative pathogen and the prevalence of bacterial vaginosis among married women patients in Kalar district. This cross-sectional study was performed from the beginning of March to the mid April-2021 among women who attended Obstetrics and Gynecological governmental hospital and out-patient clinics in Kalar City. Intra vaginal swabs have been collected in sterile Amies transport medium sticks and processed for isolation and identification of bacterial species depending on colony morphology, Gram’s stain and microbiological analysis protocols. Then socio-demographic and gynaecologic data were collected by questionnaire. Out of the 108 participant women who suffered from Gynecological diseases, 67(62.03%) of them exhibited bacterial vaginosis. From the 73 different isolated colonies, 18 bacterial species were identified; coagulase-negative staphylococci (CoNS) were the predominant cause of BV (32.84%), followed by E. coli (14.93%), Staphylococcus aureus (13.43%), Klebsiella pneumonia (8.96) and Micrococcus luteus (7.46%), while Proteus spp. and some uncommon bacteria display (1.49%) for each of them. The socio-demographic analysis between positive and negative woman patients revealed that the association between all studied risk factors and BV were statistically significant (P value < 0.05) except the age factor which was statistically non-significant meaning that the age was not associated with BV. In addition, the clinical symptom analysis showed that abnormal vaginal discharge, lower back pain, dysmenorrhea and strawberry were significantly associated with BV (P value < 0.05), while the rest of other factor did not exhibit statistically significant association.

scholarly journals Sodium sulfite research and microbiological analysis of ground beef and fresh sausage

2021 ◽  
Vol 10 (4) ◽  
pp. e20510414014
Author(s):  
Ana Jessika Santos do Nascimento ◽  
Neide Kazue Sakugawa Shinohara ◽  
Jairo Luiz de Araujo Pereira ◽  
Allan Matheus de Souza Silva ◽  
Amanda Mirelly Santos Sobral ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Sodium Sulfite ◽  
Food Additives ◽  
Ground Beef ◽  
Sodium Sulphite ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Pork Sausage ◽  
Total Coliforms ◽  
Thermotolerant Coliforms

In general, the meat contains nutrients of high biological value and susceptible to contamination by enteropathogens. One way of minimizing/eliminating this contamination is the use of food additives, in the case of meat, usually sodium sulphite. This technological resource is used to inhibit the growth of pathogenic bacteria such as Salmonella spp. and thermotolerant coliforms, mandatory parameters foreseen in sanitary legislation. Therefore, the objective of this article was to evaluate the presence of sodium sulfite and detection of Salmonella spp., total and thermotolerant coliforms in 23 samples of ground beef and 7 of fresh pork sausage, marketed in open markets and public markets of Grande Recife - PE. In a total of 30 samples analyzed, it was not detected in any presence of sodium sulphite. In the microbiological assays, also it was not detected the presence of Salmonella spp. and thermotolerant coliforms. The Total Coliforms was found in 20 meat samples (87%), at the maximum concentration of 35 MPN/g-1 and in all sausages (100%) at the concentration of 35 to 36 MPN/g-1. Although the Total Coliforms is not considered pathogenic, the presence of this indicates unsatisfactory sanitary conditions in the production, which may constitute biological risk for vulnerable groups.

scholarly journals Microbiological quality and somatic cells of in natura milk produced in Alagoas State, Brazil

2020 ◽  
Vol 9 (9) ◽  
pp. e412997379
Author(s):  
Bruno Fernando Oliveira Araújo ◽  
Sybelle Geórgia Mesquita da Silva ◽  
João Manoel da Silva ◽  
Cícero Cerqueira Cavalcanti Neto ◽  
Paula Cibelly Vilela da Silva ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Economic Value ◽  
Somatic Cells ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Psychrotrophic Bacteria ◽  
Physical Chemical ◽  
Significant Difference

Raw milk is a food with great consumption and economic value in Brazil. However, is susceptible of contamination by pathogenic bacteria. The aimed of this study was to evaluate the quality of in natura milk based on microbiological in three dairy farms, somatic cells counting (SCC), bacterial counting and his physical-chemical composition. Were made the following microbiological analysis: counting of mesophilic and psychrotrophic bacteria, coliforms at 30 ºC, coliforms at 45 ºC, Staphylococcus spp., Listeria spp., and SCC. The physical-chemical analysis was fat, protein, lactose, total solids, urea, and casein. There was no evidence of Salmonella spp. and Escherichia coli were identified in any samples.  In accordance to the microbiological standards established by Normative Instruction 76 only coliforms 30 ºC and 45 ºC counts were above the standards. There was a significant difference (p≤0.05) between the three farms studied regarding most microbiological aspects. Also, was observed difference (p≤0.05) for most of physical-chemical aspects. Overall, the milk produced in the regions of Alagoas State fails to meet just a constant criterion in the current legislation.

scholarly journals Gram Negative Bacteria Associated with Spoilage of Tomatoes Sold at Mararaba and Masaka Market, New Karu Local Government Area, Nasarawa State

2019 ◽  
pp. 1-7
Author(s):  
A. L. Olokun ◽  
A. B. Ajide ◽  
J. O. Ihuma
Keyword(s):  
Local Government ◽  
Pathogenic Bacteria ◽  
Cross Sectional Study ◽  
Local Government Area ◽  
Biochemical Test ◽  
Gram Negative Bacteria ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
Gram Negative ◽  
Esherichia Coli

Aims: The study is aimed at isolating and identifying the gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Study Design: A cross-sectional study to isolate gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Place and Duration of Study: The isolation of gram negative bacteria was carried out at the Department of Biological Sciences, Bingham University New Karu, Nasarawa State, Nigeria, between January, 2018 and September, 2018. Methodology: The samples collected were crushed with a sterile mortar and pestle after which tenfold serial dilution was carried out using sterile distilled water before inoculating on MaCconkey and Blood agar using the pour plate technique. Colonies were then subjected to gram staining and biochemical test. Results: From the result of the biochemical test, Salmonella spp, Esherichia coli spp, Klebsiella spp and vibrio spp. were the gram-negative bacteria isolated from the tomato samples collected at both markets. Conclusion: Tomatoes sold in an unclean environment that is contamination with food- borne pathogenic bacteria poses a risk to the consumers.

scholarly journals Comparative microbiological analysis of four different sea fishes collected from local market in Dhaka Metropolis

Food Research ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Ifra Tun Nur ◽  
Amatun Nur Mou ◽  
Umme Habiba
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Fecal Coliforms ◽  
Local Market ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Fish Samples ◽  
The Status ◽  
Raw Fish ◽  
Frozen Fish

The present investigation attempted to evaluate the existence and survival of spoilage microorganisms in four common sea fishes (Poma, Rupchanda, Koral and Tuna) available in Bangladesh and to determine the effects of cooking temperature to optimize the growth of fish microflora. Moreover, the status of fish in frozen condition after cooking was also studied. A total 4 categories of sea fishes were collected from the local shops in Dhaka city. Raw, cooked and frozen fish samples were analyzed for the existence of pathogenic bacteria through the conventional cultural techniques and the confirmative biochemical identification procedures. Total viable bacteria were present in all four fish samples in raw, cooked and frozen condition up to 6 log CFU/mL. Most of the raw fish samples were found to harbor a huge population of microorganisms up to 5 log CFU/mL including the fecal coliforms. Several specific bacterial species like E. coli, Klebsiella spp., Salmonella spp., Shigella spp., Staphylococcus spp., Pseudomonas spp. and Vibrio spp. were present in raw samples. However, the microbial load reduced from the fish after cooking and the status was static in frozen condition. Thus, the incidence of fecal coliforms in raw fish may be considered as a serious threat to the public health upon consumption of such fishes.

scholarly journals Study of Microbiological Quality of Fresh Juices Vended in Dhaka City

2019 ◽  
Vol 36 (1) ◽  
pp. 49-53
Author(s):  
Kayseri Afroz ◽  
Tahmina Shammi ◽  
Md Shahidul Kabir
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Antibiotic Sensitivity ◽  
Multi Drug Resistance ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendors ◽  
Shigella Spp ◽  
Vibrio Spp

Fresh juices sold by the street vendors are rich in nutritional value and considered as popular drink in Dhaka city .They can be easily contaminated by pathogenic bacteria from unhygienic environment of food preparation area and serves as a potential source of food borne outbreaks. Freshly produced juices of papaya (n = 2), wood apple (n = 2), wood apple and papaya juice (1), lemon (n = 2), and sugarcane (n = 3) were examined for the total bacterial mload, coliforms, Staphylococcus spp., Pseudomonas spp., Vibrio spp., Salmonella spp., and Shigella spp. in this study. Total bacterial load in eight samples were within 0 to 107 cfu/ml, six samples exhibited the presence of staphylococci within the range of 0 to 105 cfu/ml. Total coliforms were detected in six samples which ranged from 104 to 105 cfu/ ml. Vibrio spp., Salmonella spp., and Shigella spp. were isolated by enrichment and selective plating method. Eight samples demonstrated the presence of Vibrio spp. and none of them were positive for Salmonella spp. and Shigella spp. Antibiotic sensitivity patterns were determined against eight isolates using Erythromycin (15μg), Amoxicillin (10 μg), Gentamicin (10 μg), Vancomycin (30 μg), Ciprofloxacin (5 μg), Rifampicin (5 μg), Azithromycin (15 μg), Neomycin (10 μg) and Cefexime (5 μg). Klebsiella sp. (n = 1), Vibrio sp. (n = 2), Enterobacter sp. (n = 1), and Proteus sp. (n = 1) showed multi drug resistance in this study. Contamination of street vended juices pose risks to consumers health and requires adequate attention of appropriate authority. Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 49-53

scholarly journals Influence of Soap Characteristics and Food Service Facility Type on the Degree of Bacterial Contamination of Open, Refillable Bulk Soaps

2018 ◽  
Vol 81 (2) ◽  
pp. 218-225 ◽  
Author(s):  
Donald W. Schaffner ◽  
Dane Jensen ◽  
Charles P. Gerba ◽  
David Shumaker ◽  
James W. Arbogast
Keyword(s):  
Public Health ◽  
Antimicrobial Agent ◽  
Fast Food ◽  
Pathogenic Bacteria ◽  
Low Cost ◽  
Klebsiella Oxytoca ◽  
Food Service ◽  
Grocery Stores ◽  
Significant Difference ◽  
High Level

ABSTRACT Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella. Bacteria were screened for antibiotic resistance. The pH, solids content, and water activity of all soap samples were measured. Samples were assayed for the presence of the common antibacterial agents triclosan and parachlorometaxylenol. More than 85% of the soap samples tested contained no detectable microorganisms, but when a sample contained any detectable microorganisms, it was most likely contaminated at a very high level (∼7 log CFU/mL). Microorganisms detected in contaminated soap included Klebsiella oxytoca, Serratia liquefaciens, Shigella sonnei, Enterobacter gergoviae, Serratia odorifera, and Enterobacter cloacae. Twenty-three samples contained antibiotic-resistant organisms, some of which were resistant to two or more antibiotics. Every sample containing less than 4% solids had some detectable level of bacteria, whereas no samples with greater than 14% solids had detectable bacteria. This finding suggests the use of dilution and/or low-cost formulations as a cause of bacterial growth. There was a statistically significant difference (P = 0.0035) between the fraction of bacteria-positive samples with no detected antimicrobial agent (17%) and those containing an antimicrobial agent (7%). Fast food operations and grocery stores were more likely to have detectable bacteria in bulk-soap samples compared with convenience stores (P &lt; 0.05). Our findings underscore the risk to public health from use of refillable bulk-soap dispensers in food service establishments.

scholarly journals Microbiological analysis and determination of antimicrobial traits of green banana (Musa spp.) and papaya (Carica papaya)

2019 ◽  
Vol 8 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Nusrat Jahan ◽  
Rashed Noor ◽  
Saurab Kishore Munshi
Keyword(s):  
Pathogenic Bacteria ◽  
Carica Papaya ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Hot Water ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Musa Spp ◽  
Fruit Samples ◽  
Green Banana

Green banana (Musa spp.) and papaya (Carica papaya), which are commonly consumed as vegetables, are fruits with lots of health benefits. Due to their various medicinal applications, both the fruits are likely to contain antimicrobial properties. In this regard, present study was undertaken to determine the microbiological quality as well as the anti-bacterial traits of fresh green banana and papaya. To serve the purpose, a total of 12 samples (6 green bananas and 6 green papaya) were collected from different local and super shops as well from cultivation land of some villages of Bangladesh. The samples contained the microbial contamination with viable bacteria and fungi up to 107cfu/g and 103 cfu/g. Among the pathogenic bacteria, Staphylococcus spp. Pseudomonas spp. and Vibrio spp. were predominant as found in most of the samples in average of 102 cfu/g. Whereas Escherichia coli, Salmonella spp. and Shigella spp. were totally absent in all the samples. On the other hand, Klebsiella spp. was present in same samples. Additionally, the anti-bacterial poverties of the samples were chalked out. The in-vitro antimicrobial activities of the ethanolic, methanolic and hot water extracts of the samples were noticeably found against all the tested bacteria. Overall, present study revealed that both the green fruit samples were highly contaminated although they have antimicrobial activities. Thus, consumers’ awareness on the risk of consuming pathogen contaminated vegetables needs to be re-awakened and further investigations are required for potential antimicrobial properties. Stamford Journal of Microbiology, Vol.8(1) 2018: 41-45

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