scholarly journals Effect of Light-Emitting Diode (Led) Light on the Gene Expression Related With Ascorbate Biosynthesis and Metabolism in Broccoli Florets

2017 ◽  
Vol 2 (6) ◽  
pp. 529
Author(s):  
Chandra Kurnia Setiawan ◽  
Supriyadi Supriyadi ◽  
Umar Santoso ◽  
Gang Ma ◽  
Masaya Kato
Keyword(s):  
Gene Expression ◽  
Chlorophyll Content ◽  
Mrna Level ◽  
Red Light ◽  
Light Treatment ◽  
Rt Pcr ◽  
Light Emitting ◽  
Led Light ◽  
Red Led ◽  
Effect Of Light

Ascorbate is one of the most abundant soluble antioxidants in the plant. Multiple functions of ascorbate in photo protection have been proposed, including scavenging of reactive oxygen species generated by oxygen photoreduction and photorespiration. There is still unclear information relation to LED light with Ascorbate biosynthesis and metabolism, yellowing, chlorophyll content, and ethylene production in broccoli florets. The effect of light-emitting diodes (LED) light on ascorbate (AsA) biosynthesis and metabolism in broccoli (Brassica oleracea L. var. Italica) cultivar “Ryokurei” were studied using red (660 nm), blue (470 nm) and white LED lights as the light source and also no light treatment as the control. Gene expression involved in the biosynthesis and metabolism of AsA, AsA content, color, chlorophyll content and ethylene production rate on the postharvest broccoli were observed in 4 days. The result showed that after two days, red light treatment significantly (p < 0,05) delayed the decrease of ascorbate content. The result was supported by observations using Real-Time Quantitative RT-PCR showed that red light treatment can suppress mRNA level of BO-APX1, BO-APX2, and BO-sAPX on the third day. Observation of BO-GLDH mRNA level was increased in the third-day exposure of red LED light. Therefore red LED light showed up-regulated AsA biosynthesis transcriptional level. Enzymes which possibility responsible for AsA metabolism and biosynthesis in a row were Ascorbate Peroxide (APX) and L-Galactono-1,4-Lactone Dehydrogenase (GLDH). The regulation of this gene expression might contribute to the suppression of AsA reduction by red LED light treatment in broccoli. Red LED also showed suppression of yellowing and decline the chlorophyll content in postharvest broccoli florets. Keywords: ascorbate, LED; broccoli; gene expression; real-time quantitative RT-PCR.

scholarly journals 624 Red light emitting diode (LED) light treatment promotes memory through up-regulation of trpm4 in Zebrafish

2020 ◽  
Vol 140 (7) ◽  
pp. S84
Author(s):  
S. Yu ◽  
M. You ◽  
W. Yang ◽  
C. Cheng ◽  
H. Chang ◽  
...  
Keyword(s):  
Light Emitting Diode ◽  
Red Light ◽  
Light Treatment ◽  
Light Emitting ◽  
Led Light

PHOTOTHERAPY FOR MILD TO MODERATE ACNE VULGARIS WITH PORTABLE BLUE AND RED LED

2011 ◽  
Vol 04 (01) ◽  
pp. 45-52 ◽  
Author(s):  
GUANGDA LIU ◽  
CHANGE PAN ◽  
KAI LI ◽  
YUAN TAN ◽  
XUNBIN WEI
Keyword(s):  
Side Effects ◽  
Light Emitting Diode ◽  
Acne Vulgaris ◽  
Red Light ◽  
Light Sources ◽  
Light Emitting ◽  
Led Light ◽  
Led Phototherapy ◽  
Red Led ◽  
Complete Treatment

In this paper, we studied portable blue and red light-emitting-diode (LED) light sources in phototherapy for mild to moderate acne vulgaris to evaluate the efficacy and tolerance of patients. Patients, randomly divided into blue and red groups, received either blue or red LED phototherapy twice a week for four weeks. After complete treatment, the number of lesions reduced by 71.4% in the blue group, in contrast to 19.5% in the red group. No obvious side effects were observed during and one month after the treatment, except for some mild dryness mentioned by several patients.

scholarly journals Effects of Light-Emitting Diodes on the Accumulation of Phenolic Compounds and Glucosinolates in Brassica juncea Sprouts

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 77
Author(s):  
Chang Ha Park ◽  
Ye Eun Park ◽  
Hyeon Ji Yeo ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Brassica Juncea ◽  
Light Treatment ◽  
Total Phenolic ◽  
White Led ◽  
Light Emitting ◽  
Blue Led ◽  
Led Light ◽  
Red Led

Recent improvements in light-emitting diode (LED) technology afford an excellent opportunity to investigate the relationship between different light sources and plant metabolites. Accordingly, the goal of the present study was to determine the effect of different LED (white, blue, and red) treatments on the contents of glucosinolates (glucoiberin, gluconapin, sinigrin, gluconasturtiin, 4-methoxyglucobrassicin, 4-hydroxyglucobrassicin, glucobrassicin, and neoglucobrassicin) and phenolic compounds (4-hydroxybenzonate, catechin, chlorogenic acid, caffeate, gallate, sinapate, and quercetin) in Brassica juncea sprouts. The sprouts were grown in a growth chamber at 25 °C under irradiation with white, blue, or red LED with a flux rate of 90 μmol·m−2·s−1 and a long-day photoperiod (16 h light/8 h dark cycle). Marked differences in desulfoglucosinolate contents were observed in response to treatment with different LEDs and different treatment durations. In addition, the highest total desulfoglucosinolate content was observed in response to white LED light treatment, followed by treatment with red LED light, and then blue LED light. Among the individual desulfoglucosinolates identified in the sprouts, sinigrin exhibited the highest content, which was observed after three weeks of white LED light treatment. The highest total phenolic contents were recorded after one week of white and blue LED light treatment, whereas blue LED irradiation increased the production of most of the phenolic compounds identified, including 4-hydroxybenzonate, gallate, sinapate, caffeate, quercetin, and chlorogenic acid. The production of phenolics decreased gradually with increasing duration of LED light treatment, whereas anthocyanin accumulation showed a progressive increase during the treatment. These findings indicate that white LED light is appropriate for glucosinolate accumulation, whereas blue LED light is effective in increasing the production of phenolic compounds in B. juncea sprouts.

scholarly journals Growth and Appearance Quality of Four Microgreen Species under Light-emitting Diode Lights with Different Spectral Combinations

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1399-1405
Author(s):  
Qinglu Ying ◽  
Yun Kong ◽  
Youbin Zheng
Keyword(s):  
Plant Height ◽  
High Intensity ◽  
Light Emitting Diode ◽  
Red Light ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Light Emitting ◽  
Led Light ◽  
Red Led ◽  
Dry Biomass

To investigate plant growth and quality responses to different light spectral combinations, cabbage (Brassica oleracea L. var. capitata f. rubra), kale (Brassica napus L. ‘Red Russian’), arugula (Eruca sativa L.), and mustard (Brassica juncea L. ‘Ruby steak’) microgreens were grown in a controlled environment using sole-source light with six different spectra: 1) FL: cool white fluorescent light; 2) BR: 15% blue and 85% red light-emitting diode (LED); 3) BRFRL: 15% blue, 85% red, and 15.5 µmol·m−2·s−1 far-red (FR) LED; 4) BRFRH: 15% blue, 85% red, and 155 µmol·m−2·s−1 FR LED; 5) BGLR: 9% blue, 6% green, and 85% red LED; and 6) BGHR: 5% blue, 10% green, and 85% red LED. For all the light treatments, the total photosynthetic photon flux density (PPFD) was set at ≈330 µmol·m−2·s−1 under a 17-hour photoperiod, and the air temperature was ≈21 °C with 73% relative humidity (RH). At harvest, BR vs. FL increased plant height for all the tested species except arugula, and enlarged cotyledon area for kale and arugula. Adding high-intensity FR light to blue and red light (i.e., BRFRH) further increased plant height for all species, and cotyledon area for mustard, but it did not affect the fresh or dry biomass for any species. Also, BRFRH vs. BR increased cotyledon greenness for green-leafed species (i.e., arugula, cabbage, and kale), and reduced cotyledon redness for red-leafed mustard. However, BGLR, BGHR, and BRFRL, compared with BR, did not affect plant height, cotyledon area, or fresh or dry biomass. These results suggest that the combination of 15% blue and 85% red LED light can potentially replace FL as the sole light source for indoor production of the tested microgreen species. Combining high-intensity FR light, rather than low-level (≤10%) green light, with blue and red light could be taken into consideration for the optimization of LED light spectral quality in microgreen production under environmental conditions similar to this experiment.

scholarly journals Single Wavelengths of LED Light Supplement Promote the Biosynthesis of Major Cyclic Monoterpenes in Japanese Mint

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1420
Author(s):  
Takahiro Ueda ◽  
Miki Murata ◽  
Ken Yokawa
Keyword(s):  
Blue Light ◽  
Solid Phase ◽  
Glandular Trichomes ◽  
Red Light ◽  
Glandular Trichome ◽  
Pulse Amplitude ◽  
Light Treatment ◽  
Led Light ◽  
Trichome Formation ◽  
Japanese Mint

Environmental light conditions influence the biosynthesis of monoterpenes in the mint plant. Cyclic terpenes, such as menthol, menthone, pulegone, and menthofuran, are major odor components synthesized in mint leaves. However, it is unclear how light for cultivation affects the contents of these compounds. Artificial lighting using light-emitting diodes (LEDs) for plant cultivation has the advantage of preferential wavelength control. Here, we monitored monoterpene contents in hydroponically cultivated Japanese mint leaves under blue, red, or far-red wavelengths of LED light supplements. Volatile cyclic monoterpenes, pulegone, menthone, menthol, and menthofuran were quantified using the head-space solid phase microextraction method. As a result, all light wavelengths promoted the biosynthesis of the compounds. Remarkably, two weeks of blue-light supplement increased all compounds: pulegone (362% increase compared to the control), menthofuran (285%), menthone (223%), and menthol (389%). Red light slightly promoted pulegone (256%), menthofuran (178%), and menthol (197%). Interestingly, the accumulation of menthone (229%) or menthofuran (339%) was observed with far-red light treatment. The quantification of glandular trichomes density revealed that no increase under light supplement was confirmed. Blue light treatment even suppressed the glandular trichome formation. No promotion of photosynthesis was observed by pulse-amplitude-modulation (PAM) fluorometry. The present result indicates that light supplements directly promoted the biosynthetic pathways of cyclic monoterpenes.

scholarly journals Critical Photoperiod and Optimal Quality of Night Interruption Light for Runner Induction in June-Bearing Strawberries

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1996
Author(s):  
Yali Li ◽  
Jie Xiao ◽  
Jiangtao Hu ◽  
Byoung Ryong Jeong
Keyword(s):  
Red Light ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Light Emitting ◽  
Red Led ◽  
Critical Photoperiod ◽  
Closed Walk ◽  
White Light Emitting Diodes ◽  
Optimal Quality

The optimal photoperiod and light quality for runner induction in strawberries ‘Sulhyang’ and ‘Maehyang’ were investigated. Two experiments were carried out in a semi-closed walk-in growth chamber with 25/15 °C day/night temperatures and a light intensity of 250 μmol·m–2·s–1photosynthetic photon flux density (PPFD) provided from white light-emitting diodes (LEDs). In the first experiment, plants were treated with a photoperiod of either 12, 14, 16, 18, 20, or 22 h In the second experiment, a total of 4 h of night interruption (NI) light at an intensity of 70 μmol·m–2·s–1PPFD provided from either red, blue, green, white, or far-red LED in addition to 11 h short day (SD). The results showed that both ‘Sulhyang’ and ‘Maehyang’ produced runners when a photoperiod was longer than 16 h, and the number of runners induced positively correlated with the length of photoperiod. However, the plant growth, contents of chlorophyll, sugar and starch, and Fv/Fo decreased in a 22 h photoperiod. All qualities of the NI light, especially red light, significantly increased the number of runners and daughter plants induced per plant as compared with those in the SD treatment in both cultivars. In a conclusion, a photoperiod between 16 and 20 h and NI light, especially red NI light, can be used for quality runner induction in both ‘Sulhyang’ and ‘Maehyang’.

scholarly journals Effects of Light-Emitting Diodes on the Accumulation of Glucosinolates and Phenolic Compounds in Sprouting Canola (Brassica napus L.)

Foods ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 76 ◽  
Author(s):  
Chang Park ◽  
Nam Kim ◽  
Jong Park ◽  
Sook Lee ◽  
Jong-Won Lee ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Phenolic Compounds ◽  
Light Emitting Diode ◽  
Red Light ◽  
Dry Weight ◽  
Total Phenolic ◽  
Brassica Napus L ◽  
Light Emitting ◽  
Led Light ◽  
Sprout Growth

In this study, we investigated optimal light conditions for enhancement of the growth and accumulation of glucosinolates and phenolics in the sprouts of canola (Brassica napus L.). We found that the shoot lengths and fresh weights of red light-irradiated sprouts were higher than those of sprouts exposed to white, blue, and blue + red light, whereas root length was not notably different among red, blue, white, and blue + red light treatments. The accumulations of total glucosinolates in plants irradiated with white, blue, and red lights were not significantly different (19.32 ± 0.13, 20.69 ± 0.05, and 20.65 ± 1.70 mg/g dry weight (wt.), respectively). However, sprouts exposed to blue + red light contained the lowest levels of total glucosinolates (17.08 ± 0.28 mg/g dry wt.). The accumulation of total phenolic compounds was the highest in plants irradiated with blue light (3.81 ± 0.08 mg/g dry wt.), 1.33 times higher than the lowest level in plants irradiated with red light (2.87 ± 0.05 mg/g dry wt.). These results demonstrate that red light-emitting diode (LED) light is suitable for sprout growth and that blue LED light is effective in increasing the accumulation of glucosinolates and phenolics in B. napus sprouts.

scholarly journals Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light

2020 ◽  
Vol 8 ◽  
Author(s):  
Jaime Catalán ◽  
Marion Papas ◽  
Lina Trujillo-Rojas ◽  
Olga Blanco-Prieto ◽  
Sebastián Bonilla-Correal ◽  
...  
Keyword(s):  
Red Light ◽  
Specific Inhibitor ◽  
Motile Sperm ◽  
Light Stimulation ◽  
Led Light ◽  
Mitochondrial Atp Synthase ◽  
Red Led ◽  
Oligomycin A ◽  
Rate Of Oxygen Consumption ◽  
Stimulation Of

This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. For this purpose, freshly diluted donkey semen was stimulated with red light for 1, 5, and 10 min, in the presence or absence of oligomycin A (Omy A), a specific inhibitor of mitochondrial ATP synthase, or FCCP, a specific disruptor of mitochondrial electron chain. The results obtained in the present study indicated that the effects of red LED light on fresh donkey sperm function are related to changes in mitochondria function. In effect, irradiation of donkey sperm resulted in an increase in mitochondrial membrane potential (MMP), the activity of cytochrome C oxidase and the rate of oxygen consumption. In addition, in the absence of oligomycin A and FCCP, light-stimulation augmented the average path velocity (VAP) and modified the structure of motile sperm subpopulations, increasing the fastest and most linear subpopulation. In contrast, the presence of either Omy A or FCCP abolished the aforementioned effects. Interestingly, our results also showed that the effects of red light depend on the exposure time applied, as indicated by the observed differences between irradiation protocols. In conclusion, our results suggest that exposing fresh donkey sperm to red light modulates the function of their mitochondria through affecting the activity of the electron chain. However, the extent of this effect depends on the irradiation pattern and does not exclude the existence of other mechanisms, such as those related to thermotaxis.

Fundamentals and Applications of Red Light-Emitting Diodes (LEDs) In Vitro Plant Growth on Tomato Lycopersicon esculentum Mill

2019 ◽  
Vol 805 ◽  
pp. 141-145
Author(s):  
Nguyen Phuc Thien
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Significant Influence ◽  
Growth And Development ◽  
Red Light ◽  
Light Emitting ◽  
Red Led ◽  
In Vitro Plant ◽  
Normal Light

The aim of these studies was mainly to investigate the effects of monochromatic LEDs applied singly on the in vitro plant growth and morphogenesis. Various morphological and physiological parameters are considered that influence the growth and development of plants in vitro under red LED light as compared to those under normal light. Upon exposure to LED, in vitro-raised plants have shown significant improvements in growth and morphogenesis. In particular, red and blue lights, either alone or in combination, have a significant influence on plant growth. The present study gives an overview of the fundamentals of LEDs and describes their effects on in vitro plant growth and morphogenesis and their future potentials. The main objective of this study was to carry out line and combing ability of plant growth on tomato.

scholarly journals Effect of 660 nm Light-Emitting Diode on the Wound Healing in Fibroblast-Like Cell Lines

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Myung-Sun Kim ◽  
Yong-Ick Cho ◽  
Min-Suk Kook ◽  
Sang-Chul Jung ◽  
Young-Hyun Hwang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Near Infrared ◽  
Light Emitting Diode ◽  
Biological Effects ◽  
Soft Tissue Injuries ◽  
Light Emitting ◽  
Led Light ◽  
Red Led ◽  
And Migration

Light in the red to near-infrared (NIR) range (630–1000 nm), which is generated using low energy laser or light-emitting diode (LED) arrays, was reported to have a range of beneficial biological effects in many injury models. NIR via a LED is a well-accepted therapeutic tool for the treatment of infected, ischemic, and hypoxic wounds as well as other soft tissue injuries in humans and animals. This study examined the effects of exposure to 660 nm red LED light at intensities of 2.5, 5.5, and 8.5 mW/cm2for 5, 10, and 20 min on wound healing and proliferation in fibroblast-like cells, such as L929 mouse fibroblasts and human gingival fibroblasts (HGF-1). A photo illumination-cell culture system was designed to evaluate the cell proliferation and wound healing of fibroblast-like cells exposed to 600 nm LED light. The cell proliferation was evaluated by MTT assay, and a scratched wound assay was performed to assess the rate of migrating cells and the healing effect. Exposure to the 660 nm red LED resulted in an increase in cell proliferation and migration compared to the control, indicating its potential use as a phototherapeutic agent.

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