scholarly journals BIOCHEMICAL CHARACTERISTIC OF SOLUBLE LECTINS IN CORN UNDER THE IMPACT OF WATER DEFICIT AND HYPERTHERMIA

2021 ◽  
Vol 26 (1(48)) ◽  
pp. 7-14
Author(s):  
O. V. Ryshchakova ◽  
О. О. Molodchenkova ◽  
S. A. Petrov
Keyword(s):  
High Temperature ◽  
Drought Tolerance ◽  
Reference Value ◽  
Stress Factors ◽  
Lectin Activity ◽  
White Rats ◽  
Drought Tolerant ◽  
The Impact ◽  
The Given ◽  
Different Levels

Introduction. Drought and high temperature are two of the key factors of the environment limiting crop capacity of grains. Response of the plants to drought and high temperature is very complex and includes interaction between various molecular, physiological and biochemical processes. Synthesis of a number of proteins present under normal conditions, including lectins, increases along with synthesis of stress proteins under adverse conditions.Aim. The goal of our study is to identify the changes in the activity and biochemical characteristics of soluble lectins in maize seedlings with different drought tolerance under water and heat stress in order to create new biochemical methods for assessing drought tolerance.Methods. Three-day young sprouts of corn lines (Zea mays L.) with different levels of drought tolerance were used in the research: drought-tolerant lines Od 329, IK107 zM, non-drought tolerant lines GK 26, IK107VS3 / 66. Lectin activity was defined on the basis of their ability to agglutinate trypsinized erythrocytes of white rats. Electrophoresis was performed in 10 % PAGE following the Laemmli method.Results. The study enabled us to identify increase in soluble lectin activity (244-281 % of the reference value) under the given stress factors in drought-tolerant lines, and decrease in soluble lectin activity (39 - 79 % of reference value) under the given stress factors in non-drought-tolerant lines. Soluble lectins were isolated and purifid using salting out with ammonium sulfate, dialysis and affiity chromatography. The molecular weight of the isolated soluble lectins is in the range of 50-60 kDa. The isolated lectins had a high affiity for N-acetylglucosamine and D-fructose-6-phosphate.Conclusion. Therefore, it has been established that corn lines with positively different levels of drought tolerance are characterized by varying activity of soluble lectins.

scholarly journals Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1534
Author(s):  
Chandra Mohan Singh ◽  
Poornima Singh ◽  
Chandrakant Tiwari ◽  
Shalini Purwar ◽  
Mukul Kumar ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Crop Production ◽  
Mitigation Strategies ◽  
Theoretical Research ◽  
Whole Genome Sequence ◽  
Complex Nature ◽  
Drought Tolerant ◽  
Breeding Programmes ◽  
The Impact

Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.

scholarly journals The physiology of drought stress in grapevine: towards an integrative definition of drought tolerance

2020 ◽  
Vol 71 (16) ◽  
pp. 4658-4676 ◽  
Author(s):  
Gregory A Gambetta ◽  
Jose Carlos Herrera ◽  
Silvina Dayer ◽  
Quishuo Feng ◽  
Uri Hochberg ◽  
...  
Keyword(s):  
Climate Change ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Water Availability ◽  
Economic Importance ◽  
Fruit Crop ◽  
Crop Varieties ◽  
Drought Tolerant ◽  
Definition Of ◽  
The Impact

Abstract Water availability is arguably the most important environmental factor limiting crop growth and productivity. Erratic precipitation patterns and increased temperatures resulting from climate change will likely make drought events more frequent in many regions, increasing the demand on freshwater resources and creating major challenges for agriculture. Addressing these challenges through increased irrigation is not always a sustainable solution so there is a growing need to identify and/or breed drought-tolerant crop varieties in order to maintain sustainability in the context of climate change. Grapevine (Vitis vinifera), a major fruit crop of economic importance, has emerged as a model perennial fruit crop for the study of drought tolerance. This review synthesizes the most recent results on grapevine drought responses, the impact of water deficit on fruit yield and composition, and the identification of drought-tolerant varieties. Given the existing gaps in our knowledge of the mechanisms underlying grapevine drought responses, we aim to answer the following question: how can we move towards a more integrative definition of grapevine drought tolerance?

scholarly journals Evaluation of saline-alkali and drought tolerance in maize varieties

2019 ◽  
Vol 48 (4) ◽  
pp. 1047-1063
Author(s):  
Huili Zhang ◽  
Chuang Yuan ◽  
Guillian Mao ◽  
Xue Gao ◽  
Liu Zhu ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Growth And Development ◽  
Stress Condition ◽  
Stress Factors ◽  
Alkali Stress ◽  
Yield Attributes ◽  
Drought Tolerant ◽  
Maize Varieties

Saline-alkali and drought stresses are one of the abiotic stress factors that limit the normal growth and development of plants. In this work, various agronomic indexes including growth physiology and yield attributes were studied under saline-alkali and drought stress treatments. It was found that the limit of plant growth and development caused by drought stress is much higher than that of saline-alkali stress (p < 0.01). Based on the comprehensive evaluation value (D value), under saline-alkali stress condition, 36 maize varieties could be divided into four groups by cluster analysis (CA): High saline-alkali tolerance (3 varieties), medium saline-alkali tolerant(10 varieties), saline-alkali sensitive (19 varieties), high saline-alkali sensitive (4 varieties). In drought stress condition, 36 maize varieties could be divided into five groups by cluster analysis (CA): High drought-tolerance (2 varieties), medium drought-tolerant (14 varieties), low drought-tolerant (15 varieties), drought-sensitive (4 varieties), high drought-sensitive (1 variety). Therefore, this study provides a comprehensive screening of maize varieties under saline-alkali and drought stresses.

scholarly journals Research of psychological stability of the employees of the penal system to the influence of destructive factors of professional activity

2021 ◽  
pp. 60-70
Author(s):  
Aleksey Mikhaylov ◽  
Tat'yana Pivovarova ◽  
Ekaterina Kurkova
Keyword(s):  
Psychological Climate ◽  
Stress Factors ◽  
Psychological Characteristics ◽  
Professional Activity ◽  
Penal System ◽  
Professional Activities ◽  
The Social ◽  
The Impact ◽  
Spearman’S Correlation ◽  
Different Levels

The article presents the results of an empirical study of employees of the penitentiary system, the purpose of which was to study their psychological resistance to the impact of destructive factors of professional activity and the development of a psychocorrectional program. The professional activity of employees of the penal system is associated with the impact of various kinds of stress factors, the main of which is interaction with convicts with different levels of psychological and pedagogical neglect and criminal infection, which leaves an imprint on the employee's personality and destructive changes, and therefore the study the psychological stability of the penitentiary system employees to the impact of destructive factors of professional activity is of particular relevance for the penitentiary department. Having studied the psychological characteristics of employees and determined the destructive factors of professional activity, the authors checked the presence of a statistical relationship between the studied phenomena using Spearman's correlation coefficient, which made it possible to develop a psychocorrectional program for psychological support of this category of employees of the penal system. Also, within the framework of the developed psychocorrectional program, the main directions of work on the prevention and correction of destructive behavior are given, which in turn will increase the efficiency of the professional activities of penitentiary officials, improve the social and psychological climate within the team, and prevent discipline among the employees of the penal system.

Phytocystatins and their Potential Application in the Development of Drought Tolerance Plants in Soybeans (Glycine max L.)

2020 ◽  
Vol 27 (2) ◽  
pp. 135-144 ◽  
Author(s):  
Phetole Mangena
Keyword(s):  
Abiotic Stress ◽  
Drought Tolerance ◽  
Stress Responses ◽  
Plant Stress ◽  
Cysteine Proteases ◽  
Industrial Applications ◽  
Stress Factors ◽  
Soybean Plant ◽  
Oilseed Crop ◽  
The Impact

: Plant cystatins, also called phytocystatins constitute a family of specific cysteine protease inhibitors found in several monocots and dicots. In soybean, phytocystatins regulate several endogenous processes contributing immensely to this crop’s tolerance to abiotic stress factors. Soybeans offer numerous nutritional, pharmaceutical and industrial benefits; however, their growth and yields is hampered by drought, which causes more than 10% yield losses recorded every harvest period worldwide. This review analyses the role of papain-like cysteine proteases and their inhibitors in soybean plant growth and development under drought stress. It also describes their localisation, regulation, target organs and tissues, and the overall impact of cystatins on generating drought tolerance soybean plants. These proteins have many functions that remain poorly characterized, particularly under abiotic stress. Although much information is available on the utilisation of proteases for industrial applications, very few reports have focused on the impact of proteases on plant stress responses. The exploitation of cystatins in plant engineering, as competitive proteases inhibitors is one of the means that will guarantee the continued utilisation of soybeans as an important oilseed crop.

Photosynthetic Responses to High Temperature and Strong Light Suggest Potential Post-flowering Drought Tolerance of Sorghum Japanese Landrace Takakibi

2019 ◽  
Vol 60 (9) ◽  
pp. 2086-2099 ◽  
Author(s):  
Norikazu Ohnishi ◽  
Fiona Wacera W. ◽  
Wataru Sakamoto
Keyword(s):  
High Temperature ◽  
Drought Tolerance ◽  
Photosynthetic Rate ◽  
Photosynthetic Activity ◽  
Effective Quantum Yield ◽  
Strong Light ◽  
Fluorescence Measurements ◽  
Drought Tolerant ◽  
Photosynthetic Responses ◽  
Two Parameters

Abstract Sorghum [Sorghum bicolor (L.) Moench] is a C4 crop known to be adaptable to harsh environments such as those under high temperature and water deficit. In this study, we focused on a Japanese sorghum landrace Takakibi (NOG) and employed chlorophyll fluorescence measurements to assess its response to environmental stress. Comparison of photosynthetic rate evaluated using two parameters (effective quantum yield and electron transfer rate) indicated that NOG showed less activity than BTx623 in the pre-flowering stage, which was consistent with the higher susceptibility of NOG seedlings to drought than BTx623. The observed differences in photosynthetic activity between the two cultivars were detectable without drought conditions on days with high temperature and strong light. Interestingly, the photosynthetic activity of NOG leaves in stress conditions increased soon after heading, and the trend was similar to that in BTx642, a well-characterized post-flowering drought-tolerant cultivar. In contrast, BTx623 showed a gradual decline in photosynthetic rate. Thus, we inferred that Japanese Takakibi has the potential to show pre-flowering drought susceptibility and post-flowering drought tolerance, through which it adapts to local climates with high temperature and strong light at harvest.

scholarly journals Preharvest Aflatoxin Contamination in Drought-Tolerant and Drought-Intolerant Peanut Genotypes1

2000 ◽  
Vol 27 (2) ◽  
pp. 45-48 ◽  
Author(s):  
C. C. Holbrook ◽  
C. K. Kvien ◽  
K. S. Rucker ◽  
D. M. Wilson ◽  
J. E. Hook ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Aflatoxin Contamination ◽  
Leaf Temperature ◽  
Planting Dates ◽  
Drought Tolerant ◽  
Breeding Populations ◽  
Visual Stress ◽  
Positive Correlations ◽  
Drought And Heat Stress ◽  
Different Levels

Abstract Peanuts become contaminated with aflatoxins when subjected to prolonged periods of heat and drought stress. The effect of drought tolerance on aflatoxin contamination is not known. The objectives of this research were to evaluate preharvest aflatoxin contamination in peanut genotypes known to have drought tolerance and to determine the correlation of drought tolerance characteristics with aflatoxin contamination. Twenty genotypes with different levels of drought tolerance were grown in Yuma, AZ (a desert environment) and under rain-protected shelters in Tifton, GA. Two drought-tolerant genotypes (PI 145681 and Tifton 8) and an intolerant genotype (PI 196754) were selected for further examination in a second experiment with two planting dates in 1997 at Tifton. Drought and heat stress conditions were imposed for the 40 d preceding harvest. The drought-intolerant genotype had greater preharvest aflatoxin contamination than Florunner (the check cultivar) in the tests conducted in 1997. Both drought-tolerant genotypes had less preharvest aflatoxin contamination than Florunner in these tests. Significant positive correlations were observed between aflatoxin contamination and leaf temperature and between aflatoxin contamination and visual stress ratings. Leaf temperature and visual stress ratings are less variable and less expensive to measure than aflatoxin contamination. Leaf temperature and visual stress ratings maybe useful in indirectly selecting for reduced aflatoxin contamination in breeding populations.

scholarly journals Study of vitality as a resource for providing students’ psychological safety

2020 ◽  
pp. 4-18
Author(s):  
Olena HREK
Keyword(s):  
Statistical Analysis ◽  
Correlation Method ◽  
Psychological Safety ◽  
Stress Factors ◽  
Vitality Test ◽  
The University ◽  
The Impact ◽  
Spearman’S Correlation ◽  
Different Levels ◽  
Development Of Students

The article investigates the problem of studying the vitality according to domestic and foreign approaches. The concept of “vitality” and its components, peculiarities of vitality and stress interrelation are considered. The factors that cause stress in humans and the mechanisms of vitality that reduce the impact of stress factors on an individual, as well as characteristics of a student’s age being one of sensitive periods of their manifestation and development of vitality are shown. The author empirically investigates special features and components of vitality development of students-psychologists, who are in their 1-4 years of study at the university. The research involved 80 students from the University after Ushinsky. The following research methods were used: the vitality test by S. Maddy (adapted by D.O. Leontiev and O.I. Rasskazova), the method of life meaningful orientation by D.O. Leontiev, questionnaire “The idea of information and psychological safety” by T.O. Basanova. Spearman’s correlation method and U. Mann-Whitney’s test were used for statistical analysis. According to the results of the study, it was found that the level of the students’ vitality in their 1-4 years was average but a significant increase in indicators was found in those who were in their third year. Peculiarities of the students’ life awareness with different levels of vitality are revealed. Besides, the connections between the indicators of vitality and the components of life meaningful orientations of testees’ are presented. The level of information-and-psychological safety of students and its connection with vital indicators is investigated. That is, vitality as a resource stimulates the activity of students and can ensure their psychological safety in modern realities.

TEACH-POT: Provide Opportunities in Teaching

2020 ◽  
pp. 79-91 ◽  
Author(s):  
Maria Giulia Ballatore ◽  
Ettore Felisatti ◽  
Laura Montanaro ◽  
Anita Tabacco
Keyword(s):  
Academic Community ◽  
Real Effect ◽  
The Real ◽  
Main Components ◽  
The Impact ◽  
Training Opportunities ◽  
Different Levels

This paper is aimed to describe and critically analyze the so-called "TEACHPOT" experience (POT: Provide Opportunities in Teaching) performed during the last few years at Politecnico di Torino. Due to career criteria, the effort and the time lecturers spend in teaching have currently undergone a significant reduction in quantity. In order to support and meet each lecturers' expectations towards an improvement in their ability to teach, a mix of training opportunities has been provided. This consists of an extremely wide variety of experiences, tools, relationships, from which everyone can feel inspired to increase the effectiveness of their teaching and the participation of their students. The provided activities are designed around three main components: methodological training, teaching technologies, methodological experiences. A discussion on the findings is included and presented basing on the data collected through a survey. The impact of the overall experience can be evaluated on two different levels: the real effect on redesigning lessons, and the discussion on the matter within the entire academic community.

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