scholarly journals PHYTOMEDIATED SYNTHESIS OF SILVER NANOPARTICLES AGAINST MICROBIAL PATHOGENS AND CYTOTOXICITY ON HUMAN BREAST CANCER CELLS (MCF-7)

2020 ◽  
pp. 16-24
Author(s):  
P. Karthiga ◽  
T. Shankar ◽  
K. Karthick ◽  
K. Swarnalatha
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Silver Ions ◽  
Xrd Analysis ◽  
Vital Role ◽  
Microbial Pathogens ◽  
Root Extract ◽  
Average Size ◽  
Mcf 7 ◽  
Abutilon Indicum

Silver nanoparticles (AgNPs) are synthesized from the root extract of the Abutilon indicum plant. Nitrate reductase enzyme and/or other extra cellular proteins released from the extract reduce the silver nitrate to silver ions. These proteins or enzymes serve as a template for the silver nucleation sites in the development of silver nanoparticles, and also act as capping agents, preventing silver ion agglomeration. XRD analysis predicts the phase of the nanoparticles. Transmission Electron Microscope (TEM) and Scanning Electron Microscope analyses have revealed that the synthesized AgNPs are spherical in shape, with an average size of 17 nm. From the data, it is noted that the protein molecules and fatty acids present in the root extract of Abutilon indicum, play a vital role in reducing silver salts and as capping AgNPs at various concentrations. Bactericidal activity acting against the clinical pathogens was performed, and it was observed that NP inhibition is highly dependent on their size and surface. Cytotoxic studies were carried out with these synthesized silver nanoparticles using MTT assay on MCF-7 cells.

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals Green Synthesis of Silver Nanoparticles Using Melia Azedarach and its Characterization, Corrosion and Antibacterial Properties

2020 ◽  
Vol 11 (1) ◽  
pp. 8577-8586
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Color Change ◽  
Nitrate Solution ◽  
Antibacterial Properties ◽  
Nanoparticle Synthesis ◽  
Silver Nitrate Solution ◽  
Xrd Analysis ◽  
Melia Azedarach ◽  
Change State

Nanoparticle synthesis by the biological method is economical and environmentally friendly. In the present study, the biosynthesis of silver nanoparticles is performed by using extracts of Melia Azedarach plant leaves. The synthesis is performed by adding to the silver nitrate solution to the leaf extract of Melia azedarach. The color change state the precipitation of nanoparticles of silver. The silver nanoparticles obtained were characterized by various techniques such as X-ray diffraction (XRD), Scanning electron microscope (SEM), transmission electron microscope (TEM), and Energy dispersive spectroscopy (EDX). The XRD analysis shows the particle size of 11 nm calculated by the Debye-Scherrer. The microstructure analysis shows silver particles of spherical and triangular. The corrosion rate was found to be 0.025 mpy. The green silver nanoparticle (AgNPs) synthesized have better antimicrobial potential against both bacteria’s (Bacillus subtilis and Pseudomonas aeruginosa).

scholarly journals Antimicrobial Potential of Silver Nanoparticles Synthesized Using Medicinal Herb Coptidis rhizome

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2268 ◽  
Author(s):  
Garima Sharma ◽  
Ju-Suk Nam ◽  
Ashish Sharma ◽  
Sang-Soo Lee
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Inductively Coupled Plasma ◽  
Antimicrobial Property ◽  
Spherical Shape ◽  
Silver Ions ◽  
Average Diameter ◽  
Xrd Analysis ◽  
Visible Absorption ◽  
Biosynthesized Agnps

Coptidis rhizome contains several alkaloids that are bioactive agents of therapeutic value. We propose an eco-friendly method to synthesize biocompatible silver nanoparticles (AgNPs) using the aqueous extract of Coptidis rhizome. Silver ions were reduced to AgNPs using the aqueous extract of Coptidis rhizome, indicating that Coptidis rhizome can be used for the biosynthesis of AgNPs. The time and the concentration required for conversion of silver ions into AgNPs was optimized using UV-absorbance spectroscopy and inductively coupled plasma spectroscopy (ICP). Biosynthesized AgNPs showed a distinct UV-Visible absorption peak at 420 nm. ICP analysis showed that the time required for the completion of biosynthesis was around 20 min. Microscopic images showed that nanoparticles synthesized were of spherical shape and the average diameter of biosynthesized AgNPs was less than 30 nm. XRD analysis also confirmed the size of AgNps and revealed their crystalline nature. The interaction of AgNPs with phytochemicals present in Coptidis rhizome extract was observed in FTIR analysis. The antimicrobial property of AgNPs was evaluated using turbidity measurements. Coptidis rhizome-mediated biosynthesized AgNPs showed significant anti-bacterial activities against Escherichia coli and Staphylococcus aureus that are commonly involved in various types of infections, indicating their potential as an effective anti-bacterial agent.

scholarly journals Synthesis and Characterization of Silver Nanoparticles Using Cannonball Leaves and Their Cytotoxic Activity against MCF-7 Cell Line

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Preetha Devaraj ◽  
Prachi Kumari ◽  
Chirom Aarti ◽  
Arun Renganathan
Keyword(s):  
Silver Nanoparticles ◽  
Cell Line ◽  
Average Particle Size ◽  
Cancer Cell Line ◽  
Silver Ions ◽  
Average Particle ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Vis Spectroscopy ◽  
Mcf 7

Cannonball (Couroupita guianensis) is a tree belonging to the family Lecythidaceae. Various parts of the tree have been reported to contain oils, keto steroids, glycosides, couroupitine, indirubin, isatin, and phenolic substances. We report here the synthesis of silver nanoparticles (AgNPs) using cannonball leaves. Green synthesized nanoparticles have been characterized by UV-Vis spectroscopy, SEM, TEM, and FTIR. Cannonball leaf broth as a reducing agent converts silver ions to AgNPs in a rapid and ecofriendly manner. The UV-Vis spectra gave surface plasmon resonance peak at 434 nm. TEM image shows well-dispersed silver nanoparticles with an average particle size of 28.4 nm. FTIR showed the structure and respective bands of the synthesized nanoparticles and the stretch of bonds. Green synthesized silver nanoparticles by cannonball leaf extract show cytotoxicity to human breast cancer cell line (MCF-7). Overall, this environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster than or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods, and medical applications.

scholarly journals Green Synthesis of Silver Nanoparticles and Their Bactericidal and Antimycotic Activities against Oral Microbes

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Osvelia E. Rodríguez-Luis ◽  
Rene Hernandez-Delgadillo ◽  
Rosa Isela Sánchez-Nájera ◽  
Gabriel Alejandro Martínez-Castañón ◽  
Nereyda Niño-Martínez ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Fungal Growth ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Glycyrrhiza Glabra ◽  
X Ray Diffraction ◽  
Synthesize Silver Nanoparticles ◽  
Oral Biofilms ◽  
Oral Microbes ◽  
Average Size

Nanotechnology is a new discipline with huge applications including medicine and pharmacology industries. Although several methods and reducing agents have been employed to synthesize silver nanoparticles, reactive chemicals promote toxicity and nondesired effects on the human and biological systems. The objective of this work was to synthesize silver nanoparticles fromGlycyrrhiza glabraandAmphipterygium adstringensextracts and determine their bactericidal and antimycotic activities againstEnterococcus faecalisandCandida albicansgrowth, respectively. 1 and 10 mM silver nitrate were mixed with an extract ofGlycyrrhiza glabraandAmphipterygium adstringens.Green silver nanoparticles (AgNPs) were characterized by TEM, Vis-NIR, FTIR, fluorescence, DLS, TGA, and X-ray diffraction (XRD) analysis. Bactericidal and antimycotic activities of AgNPs were determined by Kirby and Bauer method and cell viability MTT assays. AgNPs showed a spherical shape and average size of 9 nm if prepared withGlycyrrhiza glabraextract and 3 nm if prepared withAmphipterygium adstringensextract. AgNPs inhibited the bacterial and fungal growth as was expected, without a significant cytotoxic effect on human epithelial cells. Altogether, these results strongly suggest that AgNPs could be an interesting option to control oral biofilms.

scholarly journals Copolymerization of Quinazolinone Derivatives With Styrene, Methyl Methacrylate and Their Silver Nanocomposites for Biological Applications

2021 ◽  
Author(s):  
Karim Samy El-Said ◽  
Ahmed Ahmed El-Barbary ◽  
Hazem M. ElKholy ◽  
Ahmed S. Haidyrah ◽  
Mohamed Betiha ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Methyl Methacrylate ◽  
Silver Ions ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Mice Model ◽  
Ag Nps ◽  
Silver Nanocomposites ◽  
Amorphous Structures

Reaction of 2-mercapto-3-phenylquinazolin-4(3H)-one (MPQ) with both 4-vinyl benzyl chloride and allyl bromide furnished the reactive heterocyclic monomers 3-phenyl-2-((4-vinylbenzyl) thio) quinazolin-4(3H)-one (PVTQ) and 2-(allylthio)-3-phenylquinazolin-4(3H)-one (APQ), respectively. Copolymerization of PVTQ monomer with styrene and methyl methacrylate in the presence of 2,2′-azobisisobutyronitrile (AIBN) afforded the copolymers PS-co-PPVTQ and PMMA-co-PPVTQ, respectively. Similarly, copolymerization of monomer APQ with styrene and methyl methacrylate (MMA) afforded the copolymers PS-co-PAPQ and PMMA-co-PAPQ, respectively. The resulted copolymers were characterized by using FT-IR, 1H-NMR and GPC techniques. Silver nanocomposites of PS, PMMA, PS-co-PPVTQ, PMMA-co-PPVTQ, PS-co-PAPQ and PMMA-co-PAPQ were synthesized by the addition of silver nitrate into the polymer solution. The reduction of silver ions into silver nanoparticles was performed in DMF and water. Thermogravimetric (TGA) analysis was used to determine the thermal stability of the copolymers and their silver nanocomposites. The X-ray diffraction (XRD) analysis indicated the amorphous structures of the co-polymers and confirmed the formation of silver nanoparticles. The antitumor and antibacterial activities were screened for the copolymers and enhanced by the formation of their silver nanocomposites. In vivo antitumor activity in Ehrlich Ascitic Carcinoma (EAC) mice model showed that PS-co-PPVTQ/Ag NPs, PMMA-co-PPVTQ/Ag NPs, and PMMA-co-PAPQ/Ag NPs displayed promising inhibitory effects against EAC and induce apoptosis against MCF-7 cells.

scholarly journals Multifaceted Phytogenic Silver Nanoparticles by a Rare Insectivorous Plant Drosera Spatulata Labill Var.bakoensis and its Enhanced Antioxidant, Antibacterial, Antifungal and in Vitro Cytotoxic Studies

2021 ◽  
Author(s):  
Susmila Aparna Gaddam ◽  
Venkata Subbaiah Kotakadi ◽  
Gunasekhar. Kalavakunta ◽  
Josthna Penchalaneni ◽  
Varadarajulu Naidu Challagundla ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Radical Scavenging ◽  
Human Colon ◽  
Xrd Analysis ◽  
Antimicrobial Activities ◽  
Human Colon Cancer ◽  
Tem Analysis ◽  
Insectivorous Plant ◽  
Average Size

Abstract The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var.bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of their high negative zeta potential (-34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.

Phytochemical Synthesis of Silver Nanoparticles Using Anthemis Nobilis Extract and Its Antibacterial Activity

2020 ◽  
Vol 234 (3) ◽  
pp. 531-540
Author(s):  
Saba Ghamipoor ◽  
Faeze Fayyazi ◽  
Saeed Bahadorikhalili
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Silver Ions ◽  
Good Alternative ◽  
Ft Ir ◽  
Average Size ◽  
Physical And Chemical

AbstractIn this work, green synthesis of silver nanoparticles is described by phytochemical reducing silver nitrate aqueous solution using Anthemis nobilis. For this purpose, Anthemis nobilis extract was used for the synthesis of silver nanoparticles as both surfactant and reducing agent. Green synthesis method is a good alternative to physical and chemical methods, since it is fast, simple, environmentally-friendly and economic. The produced nanoparticles are identified using FE-SEM, EDX, and FT-IR and Uv/Vis techniques. Formation of silver nanoparticles is verified in 430–420 nm range. Reduction of silver ions by hydroxyl functional group is also confirmed by FT-IR device. EDX device confirms the presence of a peak for Ag element without any impurity peak. Silver nanoparticles are identified by FE-SEM device and found to have average size between 17 and 42 nm. Also, the antibacterial activity of the synthesized nanoparticles is compared with that of staphyloccusaureus and pseudomonasa aeruginosa and the maximum inhibitory activity against the bacteria is obtained using 1 mM nitrate solution.

Study of anti-cancer properties of green silver nanoparticles against MCF-7 breast cancer cell lines

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Shweta Rajawat ◽  
Rajnish Kurchania ◽  
Katherukamen Rajukumar ◽  
Shreyas Pitale ◽  
Sonali Saha ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Cancer Cell ◽  
Black Tea ◽  
Cancer Cell Lines ◽  
Electrolytic Deposition ◽  
Anti Cancer ◽  
Average Size ◽  
Tea Leaf ◽  
Mcf 7

AbstractIn the present work, silver nanoparticles were synthesized using an easy, simple, and environment-friendly method based on principles of green chemistry in the absence of a sophisticated laboratory, and their anti-cancer properties were studied. Silver nanoparticles were synthesized using electrolytic deposition. As-synthesized nanoparticles were capped using black tea leaf extract. MTT assay was used to investigate anti-cancer activity. X-ray diffraction graphs show highly pure as-synthesized silver nanoparticles. Transmission electron microscopy images show well-dispersed spherical nanoparticles, with an average size of 9 and 15 nm, corresponding to different values of parameters used in the synthesis. For the MCF-7 cancer cell lines, 100% growth inhibition is obtained. The 50% growth inhibition concentration values against MCF-7 cancer cell lines were obtained at 70- and 30-fold dilutions of colloidal silver of almost the same concentration, 178 μg/ml, for both configurations. Silver nanoparticles can be synthesized, and their morphology can be tuned using the electrolytic deposition method with black tea leaf extract as capping agent. Silver nanoparticles with an average size of 9 nm are more effective those with an average size of 15 nm. The synthesis method is faster, cheaper, and environment friendly and renders a treatment option that can have high accessibility, reduced harmful side effects, and increased economic benefits.

scholarly journals Green Synthesis of Silver Nanoparticles Using Astragalus tribuloides Delile. Root Extract: Characterization, Antioxidant, Antibacterial, and Anti-Inflammatory Activities

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2383
Author(s):  
Majid Sharifi-Rad ◽  
Pawel Pohl ◽  
Francesco Epifano ◽  
José M. Álvarez-Suarez
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Total Phenolics ◽  
Cost Effective ◽  
Root Extract ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
High Efficient ◽  
Anti Inflammatory ◽  
Average Size

Today, the green synthesis of metal nanoparticles is a promising strategy in material science and nanotechnology. In this research, silver nanoparticles (AgNPs) were synthesized through the high-efficient, cost-effective green and facile process, using the Astragalus tribuloides Delile. root extract as a bioreduction and capping agent at room temperature. UV–Vis spectroscopy was applied for the investigation of the reaction proceedings. To characterize the greenly synthesized AgNPs, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and transmission electron microscopy (TEM) analyses were utilized. In addition, the total phenolics and flavonoids contents, antioxidant, antibacterial, and anti-inflammatory activities of the greenly synthesized AgNPs and the A. tribuloides root extract were evaluated. The results indicated that the AgNPs had spherical morphology and crystalline structure with the average size of 34.2 ± 8.0 nm. The total phenolics and flavonoids contents of the greenly synthesized AgNPs were lower than those for the A. tribuloides root extract. The resultant AgNPs exhibited the appropriate antioxidant activity (64%) as compared to that for the A. tribuloides root extract (47%). The antibacterial test approved the higher bactericidal activity of the resulting AgNPs on the Gram-positive and Gram-negative bacteria in comparison to the A. tribuloides root extract. Considering the anti-inflammatory activity, the greenly synthesized AgNPs showed a stranger effect than the A. tribuloides root extract (82% versus 69% at 500 μg/mL). Generally, the AgNPs that were fabricated by using the A. tribuloides root extract had appropriate antioxidant, antibacterial, and anti-inflammatory activities and, therefore, can be considered as a promising candidate for various biomedical applications.

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