scholarly journals Protective Effect of Naringenin on Cadmium-induced Toxicity in Rat Liver

2021 ◽  
Author(s):  
Ke Wang ◽  
Lulu Ding ◽  
Congying Kou ◽  
Ruxue Huang ◽  
Pengli Zhao ◽  
...  
Keyword(s):  
Body Weight ◽  
Protective Effect ◽  
Rat Liver ◽  
Cell Apoptosis ◽  
Structural Damage ◽  
Morphological Changes ◽  
The Body ◽  
Protective Effects ◽  
Toxic Heavy Metal ◽  
Malondialdehyde Content

Background: Cadmium (Cd) is a widespread environmental toxic heavy metal. Naringin (Nar) is reported to have a protective effect on Cd-induced liver injury. This study aimed to explore the hepatic injury induced by Cd and the protective effects of Nar on Cd-induced oxidative stress and apoptosis in rat liver. Methods: In accordance with groups, male SD rats were injected intraperitoneally with Cd and orally with Nar every day. The treatment period was 3 weeks. Body weight, the morphological changes in liver, the activity of antioxidant indices and expression of the apoptotic genes caspase-3 and -9 were assessed. Result: Results showed that the body weight of Cd-exposed rats decreased, the superoxide dismutase and catalase activities in liver decreased, the glutathione content decreased and the malondialdehyde content increased. Further, Cd-induced hepatic structural damage and cell apoptosis were observed. However, Nar could alleviate liver damage caused by Cd. Therefore, Cd caused oxidative damage and cell apoptosis in rat liver, while Nar had preventive and ameliorative effects on these injuries.

Some Indian herbs have protective effects against deleterious effects of ochratoxin A in broiler chicks

2021 ◽  
pp. 1-14
Author(s):  
S.D. Stoev ◽  
K. Dimitrov ◽  
I. Zarkov ◽  
T. Mircheva ◽  
D. Zapryanova ◽  
...  
Keyword(s):  
Body Weight ◽  
Protective Effect ◽  
Ochratoxin A ◽  
Relative Weight ◽  
Fine Powder ◽  
Inhibitory Effect ◽  
Feed Additives ◽  
Tinospora Cordifolia ◽  
Broiler Chicks ◽  
Protective Effects

A protective effect of two herbs, Glycyrrhiza glabra and Tinospora cordifolia, given as feed additives was observed against the growth inhibitory effect of ochratoxin A (OTA) and associated immunosuppression and biochemical or pathomorphological changes. The feed levels of 3 mg/kg OTA and fine powder of one of both herbs were given during a period of 32 days to female broiler chicks divided into 3 experimental and 1 control groups (14 chicks per group). The observed pathological and biochemical changes, the changes in relative organs’ weight and body weight, and the decrease of antibody titer against Newcastle disease were more pronounced in the OTA-treated chicks without herbal supplementation, and less pronounced in the chicks treated additionally with G. glabra or T. cordifolia as was shown by the better feed performance and the higher body weight in the chicks treated with the herbs. The higher relative weight of lymphoid organs of the chicks supplemented with both herbs revealed their beneficial effects on the immune system. The hepatoprotective effect of both herbs was evident, being stronger in the chicks additionally supplemented with G. glabra shown by the pathomorphological findings and by the lower levels of aspartate transaminase (131.1 U/l) compared to chicks given only OTA (156.0 U/l). A protective effect of T. cordifolia on the bone marrow and kidneys was found as was shown by the lower levels of uric acid (382.9 μmol/l) compared to chicks given only OTA (466.9 μmol/l).

Therapeutic effects of different treatment periods against ischemic stroke and toxicology study of Angong Niuhuang Pill in rats

2020 ◽  
Author(s):  
Jianzhao Chen ◽  
Yushuang Chai ◽  
Yuanfeng He ◽  
Jisheng Huang ◽  
Ting Wan ◽  
...  
Keyword(s):  
Body Weight ◽  
Ischemic Stroke ◽  
Protective Effect ◽  
Toxic Effect ◽  
Treatment Period ◽  
Ischemia Reperfusion ◽  
Neurological Function ◽  
The Body ◽  
Therapeutic Effects ◽  
Toxicology Study

Abstract Background : Angong Niuhuang Pill (ANP) is one of the most famous drugs to treat stroke in China, but there is no definite treatment period in drug instruction. In this study, we used middle cerebral artery occlusion (MCAO) model to evaluate its therapeutic effects of different treatment periods and studied its toxic effect in rats. Methods : Protective effect of ANP was observed in the cerebral ischemia-reperfusion model in rats; ANP (270 mg/kg) three different treatment period included 1 day, 4 days and 7 days. The observation period was 30 days. Therapeutic effect was evaluated by detecting neurological function, cerebral infraction volume, brain histology and cytokines. Three dose including 550, 1640, 4910 mg/kg were studied in toxicology study. The administration period was 30 days. Toxic effect was evaluated by detecting appearance, behavior, excrement character, food-intake, body weight, hematological parameters and biomarkers such as TBA, GSTα, Cystatin C, clusterin, GSH, S-100B and MBP. Results : Seven days treatment period of ANP had better effect than 1 day and 4 days treatment periods in rat MCAO model from neurological function scores, the volume of cerebral infarction, brain histology and the serum content of IL-1β, TNF-α and NO; the brain content of IL-1β and NO. The results of 30 days multiple dose toxicology study showed no animal death in all groups; in ANP 4910 mg/kg group, the kidney and liver coefficient increased about 10%, the body weight grew more slowly, the TBA increased slightly. There was no abnormal change in histology. These all recovered after drug withdraw for 8 weeks. Conclusion: Seven days treatment period of ANP had more protective effect than 1 day and 4 days treatment periods in ischemic stroke rat. No observed adverse effect level (NOAEL) of ANP was 1640 mg/kg; the safety margin of ANP was 270-1640 mg/kg. These data provided reference to modify drug instruction.

scholarly journals Prophylactic effect of vitamin E against hepatotoxicity, nephrotoxicity, haematological indices and histopathology induced by diazinon insecticide in mice

2009 ◽  
Vol 55 (3) ◽  
pp. 219-226 ◽  
Author(s):  
Nahla S. El-Shenawy ◽  
Rasha A. Al-Eisa ◽  
Fawzia El-Salmy ◽  
Omema Salah
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Kidney Function ◽  
Kidney Tissue ◽  
The Body ◽  
High Dose ◽  
Protective Effects ◽  
Histopathological Changes ◽  
Liver And Kidney ◽  
Haematological Indices

Abstract Considering that the involvement of reactive oxygen species (ROS) has been implicated in the toxicity of various pesticides, this study was designed to study the ameliorative effect of Vitamin E (100 mg/kg body weight) on mice (25 - 30 mg) treated with diazinon (32.5 or 16.25 mg/kg body weight) organophosphate insecticide for 14 days. Subchronic DZN exposure and the protective effects of vitamins E (vitE) were evaluated for their effects on haematological indices, the enzymes concerning liver damage [plasma alanine aminotransferase (ALT), aspartate aminotaransferase (AST), alkaline phosphatise (AIP), and some parameters of kidney function (urea and creatinine) in mice. Additionally, the histopathological changes in liver and kidney tissue were examined. The high dose of diazinon (DZNH) decreased the body weight significantly at the end of experiment. Additionally, the liver and kidney were examines for histopathological changes. The high dose of diazinon decreased body weight significantly. Moreover, there was a statistically significant decrease in haemoglobin (Hb), red blood cell (RBC) and hematocrit (Hct) in diazinon-treated mice compared to controls. This decrease was partially remedied in the diazinon-treated group that also received vitE. Damage in the liver and kidney tissues was also evident as elevated plasma ALT, AST, ALP, urea and creatinine. VitE partially counteracts the toxic effect of DZN and repairs tissue damage in the liver and kidney, especially when supplemented to 1/4 LD50 intoxicated animals. Histopathological changes in liver and kidney were observed only in 32.5 mg/kg DZN given group. These results suggest that the effects of DZN are dose dependent. No pathological findings were observed in vitE + DZN treated groups. According to the present study, we conclude that vitE can reduce the detrimental impacts of diazinon on haematological indicies, as well as liver and kidney function.

Protective mechanisms of protocatechuic acid against doxorubicin-induced nephrotoxicity in rat model

2019 ◽  
Vol 30 (4) ◽  
Author(s):  
Olorunfemi R. Molehin ◽  
Anne A. Adeyanju ◽  
Stephen A. Adefegha ◽  
Ajibade O. Oyeyemi ◽  
Kehinde A. Idowu
Keyword(s):  
Body Weight ◽  
Protocatechuic Acid ◽  
Serum Levels ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Protective Mechanisms ◽  
Malondialdehyde Content ◽  
Group 5 ◽  
Group 2 ◽  
Inducible Nitric Oxide

AbstractBackgroundDoxorubicin (DOX) induces toxicity in many tissues/organs, including the heart, kidney and so on. This study was designed to evaluate the modulatory effects of protocatechuic acid (PCA) against DOX-induced nephrotoxicity in rats. Animals were randomly grouped into five groups.MethodsGroup 1 served as the normal control (CTR). A single dose of DOX at 20 mg/kg was administered intraperitoneally (i.p.) to animals in Group 2. Groups 3 and 4 were pretreated with PCA for 5 days (doses of 10 and 20 mg/kg body weight, respectively) after which DOX was injected (PCA-10 + DOX and PCA-20 + DOX). Group 5 received PCA only at a dose of 20 mg/kg body weight (PCA-20).ResultsThe results revealed significant elevations (p < 0.05) in malondialdehyde content, expressions of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX2) in the kidney. Likewise, increased serum levels of creatinine and urea of DOX group were observed. A significant decrease (p < 0.05) in glutathione (GSH) level and antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione s- transferase (GST) activities in the kidney were observed compared with the control. Pretreatment with PCA (10 and 20 mg/kg, p.o.) for 5 days prior to the i.p. injection of DOX reduced MDA levels, modulated iNOS and COX2 activities and improved kidney function markers as well as oxidative stress parameters. Findings from the histopathology studies confirms the protective effects of PCA on DOX-induced damage on the kidney cells.ConclusionsThis study has demonstrated the anti-inflammatory and antioxidative properties of PCA, which could be part of its possible protective mechanisms against nephrotoxicity induced by DOX.

THE MECHANISM OF THE PROTECTIVE EFFECTS OF ADRENALINE UPON THE DIABETOGENIC ACTION OF ALLOXAN IN THE DOG

1956 ◽  
Vol 14 (3) ◽  
pp. 234-239 ◽  
Author(s):  
J. L. ARTETA ◽  
A. CARBALLIDO
Keyword(s):  
Body Weight ◽  
Protective Effect ◽  
Protective Effects ◽  
Β Cells

SUMMARY The i.v. injection of 50 μg/kg body weight of adrenaline hydrochloride prior to the injection of diabetogenic doses of alloxan has a protective effect in the dog. This protection has a duration exceeding that of the vasoconstrictor action of adrenaline. It is concluded that the protective effect may depend initially on alterations in the circulation of the pancreas brought about by adrenaline and may afterwards be maintained by the postadrenaline hyperglycaemia. The administration of 2 i.u./kg body weight of insulin by i.m. injection 2 hr before the injection of adrenaline diminishes or abolishes the protection phenomenon. This interference of insulin with the protective effect of adrenaline depends upon the hypoglycaemia which sensitizes the β-cells of the pancreas to alloxan. The i.v. injection of 2 i.u./kg body weight of insulin 5 min before the injection of adrenaline does not prevent its protective effect.

scholarly journals Investigation of the Protective Effects of Taurine against Alloxan-Induced Diabetic Retinal Changes via Electroretinogram and Retinal Histology with New Zealand White Rabbits

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Samuel Tung-Hsing Chiang ◽  
Shang-Min Yeh ◽  
Yi-Chen Chen ◽  
Shiun-Long Lin ◽  
Jung-Kai Tseng
Keyword(s):  
Body Weight ◽  
Protective Role ◽  
The Body ◽  
Antidiabetic Activity ◽  
Protective Effects ◽  
Group Iii ◽  
Glucose Levels ◽  
Diabetic Retina ◽  
Group I ◽  
Group Ii

The purpose of this study was to investigate the protective role of orally administered taurine against diabetic retinal changes via electroretinogram (ERG) and retinal histology on rabbits. Rabbits were randomly assigned into groups: Group I (vehicle administration only); Group II (diabetes: induced by 100 mg/kg alloxan injection); Group III (diabetes and fed with 200 mg/kg taurine); and Group IV (diabetes and fed with 400 mg/kg taurine). The body weight and blood glucose levels of the rabbits were monitored weekly. The ERG was measured on weeks 5 and 15. Retinal histology was analyzed in the end of the experiment. Results revealed that a taurine supplement significantly ameliorates the alloxan-induced hyperglycemia and protects the retina from electrophysiological changes. Group II showed a significant(P<0.05)change in the mean scotopic b-wave amplitude when compared to that of Group I, whereas the diabetic rabbits treated with taurine (Group III and IV) were analogous to Group I. Histologically, the amount of Bipolar and Müller cells showed no difference(P>0.05)between all groups and when compared with those of Group I. Our study provides solid evidences that taurine possesses an antidiabetic activity, reduced loss of body weight, and less electrophysiological changes of the diabetic retina.

scholarly journals Anti-Inflammatory and Antioxidative Effects of Sumatriptan Against Doxorubicin-Induced Cardiotoxicity in Rat

2021 ◽  
Author(s):  
Mohammad Sheibani ◽  
Hedyeh Faghir-Ghanesefat ◽  
Yaser Azizi ◽  
Tahmineh Mokhtari ◽  
Hasan Yousefi‐Manesh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Mortality Rate ◽  
Cardiac Tissue ◽  
The Body ◽  
Male Rats ◽  
Protective Effects ◽  
Cardiac Damage ◽  
Necrosis Factor Alpha ◽  
Antioxidative Effects

The clinical use of doxorubicin as a potent chemotherapeutic agent is limited due to its dose-dependent cardiotoxicity. Oxidative stress and inflammatory pathways have a pivotal role in doxorubicin-induced cardiotoxicity. Sumatriptan, a 5-hydroxytryptamine (5-HT)1B/1D agonist that is mainly used to relieve migraine pain, has suggested exerting protective effects in numerous pathological conditions through antiinflammatory properties. The aim of the present study was to investigate the effects of sumatriptan on doxorubicin-induced cardiotoxicity and the contribution of anti-inflammation and antioxidative responses. Cardiotoxicity was induced by the administration of doxorubicin three times a week (2.5 mg/kg i.p) for two consecutive weeks on male rats. The animals were divided into four groups, including Control, Sumatriptan (0.1 mg/kg) received group, doxorubicin received group, and Doxorubicin+Sumatriptan (0.1 mg/kg) received group. Sumatriptan was administered 30 min before every injection of doxorubicin. On the last day of the second week, the body weight, mortality rate, electrocardiogram (ECG) and histopathological changes, cardiac inotropic study, and biochemical factors were evaluated. The loss of body weight, mortality rate, ECG parameters, reduction of papillary muscle contractility force as well as histopathological scores following administration of doxorubicin indicated severe cardiac damage. However, treatment with sumatriptan inhibited the functional and structural impairment induced by doxorubicin. In addition, sumatriptan could significantly reduce cardiac tissue levels of malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α), which were increased in the doxorubicin-treated rats. This study illustrated the protective effects of sumatriptan on decreasing doxorubicin-induced cardiac toxicity and mortality rate in part through inhibition of inflammatory and oxidative stress pathways.

scholarly journals Lophira lanceolata protects testicular and spermatological damages induced by cisplatin in male Wistar rats

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Solomon Tsekohol Agu ◽  
Christian Okechukwu Ezihe ◽  
Paul Friday Itodo ◽  
Hyacinth Adakole Abu
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Antineoplastic Agent ◽  
The Body ◽  
Sperm Parameters ◽  
Protective Effects ◽  
Testicular Damage ◽  
Stress Injury ◽  
Male Wistar Rats ◽  
Lophira Lanceolata

Abstracts Background Chemotherapy is associated with male infertility. Cisplatin (CP), an antineoplastic agent has been successfully used for the treatment of diverse kinds of malignancies, however, the use of this effective agent could induce oxidative stress injury, nephrotoxicity, hepatotoxicity, and testicular damage. Combined CP chemotherapy with plant extracts can diminish the toxicity and enhance the antitumor efficacy of the drug. The objective of the study was to determine the protective effect Lophira lanceolata leaf extract (LLLE) on CP-induced toxicity on male reproductive organs. Methods The study was carried out with 30 (n = 30) male Wistar rats (Rattus norvegicus). The rats were randomly assigned into 6 groups of 5 rats each. Rats in group 1 (Control) were administered distilled water per os. Rats in group 2 were administered 5 mg/kg of CP intraperitoneally (i.p). Rats in groups 3 and 4 were administered per os LLLE at the doses of 200 and 400 mg/kg body weight and rats in groups 5 and 6 were administered 5 mg/kg body weight of CP + LLLE at the doses of 200 and 400 mg/kg body weight respectively. Results The results showed a significant decrease in the sperm parameters in the group treated with CP alone when compared with the control and there in the sperm parameters in the groups administered CP + LLLE. The body and organ weights of the rats were significantly (p < 0.05) decreased in the CP treated group relative to the control. However, there was an increase in the weight of the organs in the LLLE pretreated groups. The photomicrographs showed degenerative changes in the testicular tissues of the rats administered CP alone whereas the group pretreated with the LLLE showed amelioration induced by the CP. Our study revealed that CP treatment has deleterious effects on sperm parameters and testicular tissues and the accessory sex organs (Epididymis, prostate, seminal vesicles) of the rats. Oral administration of LLLE at 200 and 400 mg/kg bodyweight for 26 days conferred protective effects against testicular damage induced by CP. Conclusion This study revealed that pretreatment with LLLE protected against CP-induced testicular toxicity.

Protective effect of nitronyl nitroxide against hypoxia-induced damage in PC12 cells

2020 ◽  
Vol 98 (3) ◽  
pp. 345-353 ◽  
Author(s):  
Hongbo Luo ◽  
Wei Sun ◽  
Jin Shao ◽  
Huiping Ma ◽  
Zhengping Jia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pc12 Cells ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Morphological Changes ◽  
Radical Scavenging ◽  
Protective Effects ◽  
Nitronyl Nitroxide ◽  
Promising Candidate ◽  
Radical Scavenging Properties

Hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. HPN (4′-hydroxyl-2-substituted phenyl nitronyl nitroxide), a stable nitronyl nitroxide, has excellent free radical scavenging properties. The purpose of this study was to investigate the protective effects of HPN on hypoxia-induced damage in PC12 cells. It was shown that HPN significantly attenuated hypoxia-induced loss of cell viability, release of lactate dehydrogenase (LDH), and morphological changes in PC12 cells. Moreover, hypoxic PC12 cells had increased levels of reactive oxygen species (ROS), malondialdehyde (MDA), and expression of HIF-1α and VEGF, but had reduced levels of superoxide dismutase (SOD) and catalase (CAT), and HPN reversed these changes. HPN also inhibited hypoxia-induced cell apoptosis via suppressing the expression of Bax, cytochrome c, and caspase-3, and inducing the expression of Bcl-2. These results indicate that the protective effects of HPN on hypoxia-induced damage in PC12 cells is associated with the suppression of hypoxia-induced oxidative stress and cell apoptosis. HPN could be a promising candidate for the development of a novel neuroprotective agent.

scholarly journals Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Amanda Natália Lucchesi ◽  
Lucas Langoni Cassettari ◽  
César Tadeu Spadella
Keyword(s):  
Body Weight ◽  
Rat Liver ◽  
Morphological Changes ◽  
Glycosylated Hemoglobin ◽  
Weight Ratio ◽  
Liver Weight ◽  
Fatty Degeneration ◽  
High Serum ◽  
Ultrastructural Changes ◽  
Long Term Effects

Purpose. This study evaluated the long-term effects of alloxan-induced diabetes in rat liver.Methods. Thirty nondiabetic control rats (NC) and 30 untreated diabetic (UD) rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Clinical and laboratory parameters were assessed. Fresh liver weight and its relationship with body weight were obtained, and liver tissue was analyzed.Results. UD rats showed sustained hyperglycemia, high glycosylated hemoglobin, and low plasma insulin. High serum levels of AST and ALT were observed in UD rats after 2 weeks, but only ALT remained elevated throughout the experiment. Fresh liver weight was equal between NC and UD rats, but the fresh liver weight/body weight ratio was significantly higher in UD rats after 14 and 26 weeks. UD rats showed liver morphological changes characterized by hepatic sinusoidal enlargement and micro- and macrovesicular hepatocyte fatty degeneration with progressive liver structure loss, steatohepatitis, and periportal fibrosis. Ultrastructural changes of hepatocytes, such as a decrease in the number of intracytoplasmic organelles and degeneration of mitochondria, rough endoplasmic reticulum, and nuclei, were also observed.Conclusion. Alloxan-induced diabetes triggered liver morphological and ultrastructural changes that closely resembled human disease, ranging from steatosis to steatohepatitis and liver fibrosis.

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