Increase nutritional content and in vitro digestibility of forage legumes by adding molybdenum with foliar spray methods

Author(s):  
Iin Susilawati ◽  
Nyimas Popi Indriani ◽  
Lizah Khairani ◽  
U. Hidayat Tanuwiria

Legumes as a source of protein is one of the most important nutrients for the ruminants. The experiment was carried out in the field of Forage Crop Laboratory of Padjadjaran University from october 2016 until January 2017, to study the effect of molybdenum fertilization on quality and in vitro digestibility on legumes. The design used was randomized block design with six treatment combinations consisting two levels of molybdenum (0 and 1.5 kg Mo ha-1) with foliar spray methods and three species of legumes (Pueraria phaseoloides, Calopogonium mucunoides and Centrosema pubescens) replicated four times. The results showed that the type of legume and molybdenum fertilizer had significant effect on crude protein content, ash content and in vitro digestibility but no significant effect on crude fiber content of forage legume.

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 


2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.


2018 ◽  
Vol 28 (3) ◽  
pp. 213
Author(s):  
Eko Marhaeniyanto ◽  
Sri Susanti

The aim of this research was to study the in vitro ruminal fermentability of supplementation of <em>Gliricidia sepium, Moringa oleifera</em>, Lamk (<em>MOL</em>), <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed to be tested on sheep.<strong> </strong>This research with the experimental methods was arranged in Randomized Block Design (RBD) with 6 treatments and 3 groups. The treatment feed tested consisted of concentrate without leaves and concentrate with leaf meal supplementation. Crude protein content (CP) concentrates were prepared 16%, 18% and 20%. Supplementation uses a mixture of <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed (1: 1: 1: 1) as much as 10%, 20% and 30%. The measured variables were  degradation of dry matter (DDM) and degradation of organic matter (DOM), gas production rate, microbial biomass and NH<sub>3</sub> concentration. The use of mixed leaf meal in concentrate feed resulted in in vitro DDM as well as concentrate feed without leaf meal supplementation. Supplementation of leaf meal in concentrate feed as much as 30% with CP 20% resulted in a decrease in the value of fermentability. The supplementation of leaf meal in concentrate feed as much as 20% in concentrate feed with CP 18% produced the best fermentability value. Suggested for in-vivo trials on sheep using 18% protein concentrate feed by utilizing <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed as cheap protein sources.


Author(s):  
La Malesi ◽  
Takdir Saili ◽  
Ali Bain ◽  
Tresjia C Rakian

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (<em>p</em><0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (<em>p</em>>0.05), whereas among the defoliation age treatments were significantly different (<em>p</em><0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.


2022 ◽  
Vol 335 ◽  
pp. 00047
Author(s):  
Poespitasari Hazanah Ndaru ◽  
Siti Chuzaemi ◽  
Muhimmatu Mufidah

The purpose of this research was to determine the influence of Myristic acid and the source of condensed tannin on corn straw basedcomplete feed on nutrient content and in vitro digestibility. The method which was used in this experiment was randomized block design with four treatments and three replications. The treatment consisted of T0 complete feed (40% corn straw + 60% concentrate), T1 complete feed (40% corn straw + 60% concentrate + myristic acid 30 g/Kg DM), T2 complete feed (40% corn straw + 50% concentrate + calliandra leaf meal 10%/Kg DM and myristic acid 30 g/Kg DM), T3 complete feed (40% corn straw + 45% concentrate + calliandra leaf meal 15%/Kg DM and myristic acid 30 g/Kg DM), T4 (40% corn straw + 40% concentrate + calliandra leaf meal 20%/Kg DM and myristic acid 30 g/Kg DM). Based on the result, it could be concluded that calliandra leaf meal 15%/Kg DM + Myristic acid 30 g/Kg DM on the complete feed (T3) was the best treatment based on nutrient content and increase of dry matter digestibility, but not an effect of organic matter digestibility


Author(s):  
Daniele Cristina PEREIRA ◽  
Rafael Henrique de Tonissi e Buschinelli de GOES ◽  
Antônio Campanha MARTINEZ ◽  
Jefferson Rodrigues GANDRA ◽  
Eduardo PRESENDO ◽  
...  

ABSTRACT This study evaluated the in vitro digestibility of nutrients from different diets added with chitosan (Q), technic cashew nut shell liquid (LCC) and the association of Q and LCC. The treatments used consisted of 4 diets (forage: concentrate ratio of 100: 0, 50:50, 40:60 and 20:80) associated with 4 additives (control, chitosan, LCC and the association of Q + LCC), totaling 16 treatments, in a 4x4 factorial randomized block design. The dosages used were: Control (without additives), LCC (600mg/kg DM), Chitosan (900mg/kg DM), and LCCQ (600mg/kg LCC DM + 900mg/kg Chitosan DM). In the laboratory, samples were analyzed for IVDMD, IVNDFD, IVCPD, pH and RAN (ruminal ammonia nitrogen). For pH and RAN analyses, samples were taken at 0, 2, 4, 6 and 8 hours after incubation. The results showed higher digestibility of DM, NDF and CP for diets with chitosan and technic cashew nut shell liquid alone and higher pH and RAN values in the diets containing the two additives. The association of additives brings better results for animal nutrition and increases ruminant productivity.


2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Edenilson Meurer ◽  
Sara Carvalho de Brito ◽  
Sidnei Roberto de Marchi ◽  
Guilherme Henrique Rodrigues Pinheiro ◽  
Dagoberto Martins

The increased world population has raised the demand for animal proteins, forcing livestock to become productive, efficient, and environmentally sustainable. This study aimed to assess the effect of weed coexistence on productivity, nutritional quality, and potential production of greenhouse gases (GHG) by Convert HD364 grass (Urochloa hibrida cv. Mulato II) under pasture renovation conditions. The experiment was carried out in a randomized block design with four replications and treatments consisting of eight coexistence periods: 0, 15, 30, 45, 60, 75, 90, and 120 days after seedling emergence. Phytosociological assessments were performed in the weed community at the end of each coexistence period. The Convert HD364 grass was assessed for the morphological and chemical-bromatological parameters and for the GHG emission potential. Productivity, nutritional quality, and energy value of the Convert HD364 grass decreased proportionally with the increased period of weed coexistence. The non-coexistence with weeds results in a better in vitro digestibility of organic matter and, consequently, decreases the potential of GHG emission by the dry matter of the Convert HD364 grass.


2022 ◽  
Vol 951 (1) ◽  
pp. 012013
Author(s):  
A Rosmalia ◽  
Astriani ◽  
W P Sahroni ◽  
I G Permana ◽  
Despal

Abstract The availability of nitrogen and sulfur in the rumen should be synchronized to generate microbial protein synthesis. This study aimed to evaluate rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation. The experimental design was a 4 x 3 factorial randomized block design. Factor 1 was RDP levels (%CP) (R1= 60%; R2= 55%; R3= 50%; R4= 45%), and factor 2 was sulfur supplementation (S1= 0%; S2= 0.1%; S3= 0.2%). Data were analyzed with ANOVA followed by the Duncan test. The result showed that sulfur supplementation improved DMD and OMD without changes in rumen pH. NH3 concentration was influenced by RDP levels. Treatment R1 had the highest NH3 concentration (7.03 mM). There was an interaction of two factors on total VFA concentration. The combination of R1 and S2 resulted in the highest total VFA concentration than others. The total bacteria population increased with increasing RDP levels. Protozoa population was affected by RDP levels and sulfur supplementation. This study concluded that a combination of 60% RDP and 0.2% sulfur supplementation resulted in the best ration to improve ruminal fermentation and digestibility.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 404-404
Author(s):  
Leeroy A Lente ◽  
Roderick A Gonzalez Murray ◽  
Shawn L Archibeque

Abstract The aim of this study was to assess the effects of Zeolite (ZE, clinoptilolite) inclusion on in vitro rumen fermentation. A modified procedure from Tilley and Terry (1963) was used to determine alfalfa in vitro dry matter digestibility (IVDMD) in the presence or absence of ZE. Test tubes (n = 96) were placed in a 39 °C bath and were blocked based on hours 0, 1, 2, 4, 6, 8, 12, 24, and 48. Substrate consisted of dried alfalfa grinded through a 1mm screen using a Wily Mill. Treatments consisted of, Control (no ZE inclusion), and 0.05g ZE, for each 1 gram of alfalfa. After incubation, IVDMD was calculated 24 hours after drying period. Data was analyzed using a randomized block design. The IVDMD was found to be similar across all treatments (P &gt; 0.8464). The IVDMD was significantly different when incubated for the varying lengths of time (P &lt; 0.001). There was an IVDMD of 20.18 ± 2.89 % for the control and 18.07 ± 5.49 % for the ZE at the zero hour. The IVDMD for Control and ZE for 48 hours were 54.30 ± 1.58 % and 53. 48 ± 1.04 % respectively. To predict the IVDMD of the effects of ZE treatment on digestibility over time, the corresponding regression f(x) = 20.60 + 0.421X1 + 0.730X2 with R2 = 0.8464. In conclusion these data demonstrate that inclusion of ZE, does not influence the in vitro digestibility of alfalfa. These findings are important as previous data would indicate that inclusion of ZE may reduce cost of gain and/or influence liver abscess rates, it was uncertain if that would come at the cost of reduced digestibility of feed. These data indicate that there is likely very little to no impact on feed digestibility when ZE is included in the ration.


1966 ◽  
Vol 46 (2) ◽  
pp. 101-109 ◽  
Author(s):  
M. Hidiroglou ◽  
P. Dermine ◽  
H. A. Hamilton ◽  
J. E. Troelsen

Seasonal changes in the proximate constituents and in vitro digestibility of timothy and alfalfa were recorded at Kapuskasing during the 1963 growing season. A 20-week experiment extending from May 21 to October 8 was divided in four growing periods corresponding to various temperature regimes. There was a highly significant negative correlation between the percentage of crude fiber and that of crude protein and a significant positive correlation between protein content and in vitro digestibility. In the fall, digestibility and crude protein level showed the highest values while a corresponding significant drop was found in the crude fiber content. The percentage of crude protein and the digestibility of both dry and organic matter of alfalfa declined and the percentage of crude fiber increased with advance in maturity during all growth periods. The same trend was observed for timothy except in the fall, when digestibility and crude fiber showed no consistent fluctuations with advancing maturity. Changes in the crude fiber, fat, and ash and digestibility of timothy were smaller than in alfalfa, particularly in the second half of the season. Best yields of digestible dry matter in the first growth cycle were obtained by cutting on July 9, while best regrowth yields were reported in late summer–early fall.Temperature and precipitation in the week preceding each cut had no consistent influence on the chemical composition and digestibility.


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