ANTIBACTERIAL ACTIVITY TEST SEA CUCUMBER EXTRACT (Holothuria scabra) SIDAYU COAST GRESIK USING DISK DIFFUSION METHOD
<p>Sea cucumber (<em>Holothuria scabra</em>) is one kind of sea cucumbers that contains of bioactive compound that can inhibit bacteria growth. This research aim to know the effect of methanol and <em>n</em>-hexane extract towards bacterium <em>Staphylococcus aureus, Salmonella typhi </em>and<em> Escherichia coli</em> bacterial growth and to identification estimation active compound group.</p><p>Sea cucumbers active compound extraction using maceration method with methanol and <em>n</em>-hexane as a solvent. Methanol and <em>n</em>-hexane crude extract conducted antibacterial assay using disk diffusion method towards bacterium <em>Staphylococcus aureus, Salmonella typhi, Escherichia coli</em> and continued with reagent test to knowing active compound group.</p>The result of this research indicated that <em>n</em>-hexane extracts provide the best inhibition zone toward bacterium <em>Escherichia coli</em> at concentration 2000 ppm with among of inhibition zone 1,75 mm, while at bacterium <em>Staphylococcus aureus</em> and <em>Salmonella typhi</em> does not provide inhibition zone. Methanol extract does not provide inhibition zone towards bacterium <em>Staphylococcus aureus, Salmonella typhi</em> and <em>Escherichia coli</em>. The result of reagent test indicated compound that detected in <em>n</em>-hexane extract is saponins