The evaluation of different levels diets protein for growth performance of Clarias sp. fry cultured in biofloc-based system

ABSTRACT The study was conducted to evaluate the performance of catfish fry grown using different dietary protein levels in the biofloc-base aquaculture system. Experiments using a completely randomized design, consisted of four treatments and three replications. The treatment consisted of: protein 38% (A), 34% protein (B), 30% protein (C), and protein 26% (D). Catfish with initial weight of 0.83±0.01 g and length of 4.64±0.04 cm were cultured in 60 L tank with density of 90 fish each tank for 35 days. Inoculation of heterotrophic bacterial Staphylococcus lentus L1k were performed of 104 CFU/mL ratio of 15 was administrated once a day after two hours feeding in the morning. Feeding was conducted twice a day at 5% of the biomass weight. At the end of trial treatment (D) showed the highest survival rate (88.15±5.25%), the body lenght variance coefficient (9.58±0.51%) and protein retention (39.87±2.77%). Treatment (B) showed the highest growth rate (4.11±0.05%), total length (2.39±0.08 cm), and feed intake (318.76±4.63). Treatment (A) showed the highest feed efficiency (93.65±4.43%) while lowest lipid retention compared to others (22.20±1.20%.). Based on the results, it can be concluded that 34% protein feed (C) can replace 38% protein feed (B) catfish fry size 4−5 cm through biofloc-based system. Keywords: biofloc, fry, growth, protein, Staphylococcus lentus L1k ABSTRAK Penelitian bertujuan mengevaluasi kinerja pertumbuhan benih lele dengan menggunakan kadar protein pakan yang berbeda pada sistem bioflok. Percobaan dilakukan menggunakan rancangan acak lengkap, terdiri atas empat perlakuan dan tiga ulangan. Perlakuan terdiri atas protein 38% (A), protein 34% (B), protein 30% (C), dan protein 26% (D), terdiri atas empat perlakuan dan tiga ulangan. Benih lele dengan berat rata-rata 0,83±0,01 g dan panjang rata-rata 4,64±0,04 cm dipelihara di akuarium berukuran 90×50×40 cm3 dengan padat tebar 90 ekor/akuarium selama 35 hari. Inokulasi bakteri heterotrof berupa Staphylococcus lentus L1k dilakukan pada setiap perlakuan kepadatan 104 CFU/mL. Penambahan sumber karbon berupa molase dengan C/N (Carbon/Nitrogen) rasio 15 diberikan satu kali sehari setelah dua jam pemberian pakan di pagi hari. Pakan diberikan dua kali sehari sebanyak 5% dari berat biomasa. Hasil penelitian menunjukkan pemberian kadar protein pakan berbeda memberikan hasil yang positif. Perlakuan (D) menunjukkan tingkat kelangsungan hidup (88,15±5,25%), koefisien keragaman panjang (9,58±0,51%) dan retensi protein (39,87±2,77%) terbaik. Perlakuan (B) menunjukkan laju pertumbuhan harian (4,11±0,05%), panjang total (2,39±0,08 cm), dan jumlah konsumsi pakan (318,76±4,63) tertinggi. Perlakuan (A) menunjukkan efisiensi pakan (93,65±4,43%) tertinggi namun menunjukkan retensi lemak (22,20±1,20%) terendah. Berdasarkan hasil penelitian disimpulkan bahwa pemberian pakan dengan kadar protein 34% mempunyai performa pertumbuhan yang sama dibandingkan protein 38% pada benih ikan lele berukuran 4−5 yang dipelihara menggunakan sistem budidaya bioflok. Kata kunci: benih lele, protein, pertumbuhan, bioflok, Staphylococcus lentus L1k

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The evaluation of different levels diets protein for growth performance of Clarias sp. fry cultured in biofloc-based system

Jurnal Akuakultur Indonesia ◽

10.19027/jai.16.2.147-154 ◽

2017 ◽

Vol 16 (2) ◽

pp. 147

Author(s):

Noviati Rohmatul Khasanah ◽

Nur Bambang Priyo Utomo ◽

Mia Setiawati ◽

Munti Yuhana

Keyword(s):

Feed Efficiency ◽

Protein C ◽

The Body ◽

Protein Retention ◽

Protein Levels ◽

Randomized Design ◽

Trial Treatment ◽

Completely Randomized Design ◽

Protein Feed ◽

Different Levels

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Cellulase hydrolyzed Indigofera zolingeriana leaf utilization as a feed ingredient for gourami fingerling

Jurnal Akuakultur Indonesia ◽

10.19027/jai.20.2.139-147 ◽

2021 ◽

Vol 20 (2) ◽

pp. 139-147

Author(s):

Jefry Jefry ◽

Mia Setiawati ◽

Dedi Jusadi ◽

Ichsan Achmad Fauzi

Keyword(s):

Growth Performance ◽

Feed Efficiency ◽

Specific Growth ◽

Second Step ◽

Feed Ingredient ◽

Protein Retention ◽

Cellulase Enzyme ◽

Randomized Design ◽

Best Value ◽

Completely Randomized Design

This study was aimed to evaluate the utilization of hydrolyzed Indigofera zolingeriana by celullase enzyme as the feed ingredient of gourami fish. This study used a completely randomized design which contained three steps, whereas each step contained four treatments and four replications. The first step performed by evaluating the Indigofera leaf meal (ILM) added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of ILM on gourami seeds. The third step was feed evaluation added with ILM as much as 0% (control), 15%, 30%, and 45% against the growth performance on gourami seeds. The gourami seeds used in the second and third steps with a weight of 13.65 ± 0.39 g/seed and 5.95 ± 0.15 g/seed, respectively. The addition of 0.8 g/kg and 1.2 g/kg cellulase enzyme could significantly decrease the crude fiber of ILM with 43.33%, besides having the best value of total, ingredient, protein, lipid, and energy digestibility. The growth performance of gourami seeds given 15% ILM added feed had the best value and insignificantly different from the control feed without ILM addition based on the specific growth rate (SGR), protein retention (PR), and feed efficiency (FE). Keywords: Cellulase, feed, hydrolyze, Indigofera zolingeriana, Osphronemus gouramy. ABSTRAK Penelitian ini dilakukan untuk mengevaluasi pemanfaatan daun Indigofera zolingeriana yang dihidrolisis enzim selulase sebagai bahan baku pada pakan benih ikan gurami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas tiga tahap, dimana masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Pada tahap pertama dilakukan evaluasi tepung daun Indigofera (TDI) yang ditambahkan enzim selulase sebesar 0 g/kg (kontrol), 0.4 g/kg, 0.8 g/kg, dan 1.2 g/kg. Pada tahap kedua dilakukan uji kecernaan bahan TDI pada benih ikan gurami. Pada tahap ketiga dilakukan evaluasi pakan yang ditambahkan TDI sebesar 0% (kontrol), 15%, 30% dan 45% terhadap kinerja pertumbuhan pada benih ikan gurami. Benih ikan gurami yang digunakan pada penilitian tahap kedua dengan bobot 13.65 ± 0.39 g/ekor dan 5.95 ± 0.15 g/ekor. Penambahan enzim selulase pada dosis 0.8 g/kg dan 1.2 g/kg secara signifikan mampu menurunkan serat kasar TDI sebesar 43.33 % dan memberikan nilai terbaik terhadap nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Kinerja pertumbuhan benih ikan gurami yang diberikan pakan yang ditambahkan TDI sebesar 15% memilki nilai terbaik dan tidak berbeda nyata terhadap pakan kontrol tanpa TDI dari aspek laju pertumbuhan spesifik (LPS), retensi protein (RP) dan efisiensi pakan (EP). Kata kunci: Hidrolisis, Indigofera zolingeriana, Osphronemus gouramy, pakan, selulase.

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KINERJA PERTUMBUHAN DAN EFISIENSI PAKAN IKAN Tor tambroides YANG DIBERI PAKAN KOMERSIAL DENGAN KANDUNGAN PROTEIN BERBEDA

Media Akuakultur ◽

10.15578/ma.12.1.2017.27-33 ◽

2017 ◽

Vol 12 (1) ◽

pp. 27

Author(s):

Deni Radona ◽

Jojo Subagja ◽

Irin Iriana Kusmini

Keyword(s):

Feed Efficiency ◽

Average Length ◽

Protein A ◽

Stocking Density ◽

Feed Conversion ◽

Protein Levels ◽

Randomized Design ◽

Completely Randomized Design ◽

Feed Protein ◽

C 50

Protein merupakan nutrien yang sangat berperan dalam pertumbuhan ikan. Penelitian ini bertujuan mengetahui kebutuhan protein optimal untuk pertumbuhan ikan Tor tambroides dan pengaruh kandungan protein pakan terhadap efisiensi pakannya. Benih ikan Tor yang digunakan berukuran panjang (1,5 ± 0,1 cm) dan bobot (0,08 ± 0,01 g). Benih ikan dipelihara dalam akuarium berukuran 40 cm x 30 cm x 30 cm dengan ketinggian air 20 cm sebanyak 50 ekor. Selama 40 hari pemeliharaan benih ikan diberi pakan komersil berupa crumble dengan kandungan protein, (A) 25%, (B) 35%, dan (C) 50% sebanyak 20% per hari dari total biomassa ikan, pemberian pakan dengan frekuensi tiga kali sehari. Rancangan penelitian menggunakan rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan. Hasil penelitian menunjukkan ikan Tor tambroides yang diberi pakan dengan kandungan protein sebesar 35% dan 50% memiliki pertumbuhan panjang, bobot, laju pertumbuhan harian (LPH), biomassa, nisbah konversi pakan (FCR), dan efisiensi pakan yang sama (P>0,05) dan berbeda nyata pada pakan dengan kandungan protein 25% (P<0,05).Protein is a nutrient is which plays a major role in the growth of fish. This study was aimed to determine the optimal protein requirement for growing of Thai mahseer and the influence of different protein levels of feed on feed efficiency. The average length and weight of fish used were 1.5 ± 0.1 cm and 0.08 ± 0.01 g. The fish were reared in the aquarium (dimension= 40 cm x 30 cm x 30 cm filled with 20 cm of water and the stocking density of each aquarium was 50 individuals. During 40-day reared, seedling fish were fed by commercial crumble with different protein levels, (A) 25%, (B) 35%, and (C) 50%, as much as 20% of total weight every day with a feeding frequency of three times per day. This experiment was conducted by using completely randomized design (CRD) with three treatments and three replications for each treatment. The results showed that Tor tambroides fed diets with protein levels of 35% and 50% was not significantly different on the growth value (length and weight), specific growth rate, biomass, feed conversion ratio (FCR), and feed efficiency (P>0.05) and was significantly different on feed protein levels 25% (P<0.05).

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THE EFFECT OF L-LYSINE HCL SUPPLEMENTATION ON THE BODY WEIGHT GAIN, FEED CONSUMPTION AND FEED EFFICIENCY OF BROILERS

Journal of Tropical Animal Science and Technology ◽

10.32938/jtast.v2i2.582 ◽

2020 ◽

Vol 2 (2) ◽

pp. 8-16

Author(s):

Agustina Foni ◽

Charles Venirius Lisnahan ◽

Oktovianus Rafael Nahak

Keyword(s):

Body Weight ◽

Weight Gain ◽

Feed Efficiency ◽

Body Weight Gain ◽

Animal Husbandry ◽

The Body ◽

Feed Consumption ◽

Study Program ◽

Randomized Design ◽

Completely Randomized Design

The purpose of this study was to determine the effect of l-lysine supplementation in feed on the body weight gain, feed consumption, and feed efficiency of broilers. The experiment was conducted at the Laboratory of Animal Husbandry Study Program, Agriculture Faculty, University of Timor, Kefamenanu from December 2019 to January 2020. This study used 80 day-old chicks (DOC) broilers. The method used is a Completely Randomized Design with four treatments and four replications. The treatments give were: T0 (control feed), T1 (control feed + 0.35% l-lysine), T2 (control feed + 0.70% l-lysine), T3 (control feed + 1.00% l -lysine). Data were processed using Variance Analysis, and Duncan test. The results of the statistical analysis showed that the supplementation of l-lysine in the feed had a significant effect on weight gain, feed consumption, and feed efficiency (P<0.05). It was concluded that supplementation of 0.70% l-lysine HCl in feed gave optimum results to weight gain, feed consumption, and feed efficiency of broilers.

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Effect of nutrition on the body temperature and relative organ weights of broilers

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4575 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4575

Author(s):

Julyana Machado da Silva Martins ◽

Evandro De Abreu Fernandes ◽

João Paulo Rodrigues Bueno ◽

Carolina Magalhães Caires Carvalho ◽

Fernanda Heloisa Litz ◽

...

Keyword(s):

Small Intestine ◽

Body Temperature ◽

Energy Levels ◽

Liver Weight ◽

The Body ◽

Body Temperatures ◽

Average Surface ◽

Randomized Design ◽

Males And Females ◽

Completely Randomized Design

The objective of this study was to evaluate the effect of different nutritional plans on the body temperature and organ biometrics in male and female broilers, of two ages. Here, 1,700 birds were used (850 males and 850 females) in a completely randomized design composed of five treatments (- 3%, - 1.5%, reference, + 1.5% and + 3%), with 10 repetitions, totaling 50 experimental units; the reference treatment based on nutritional and energy levels indicated in previous studies was calculated from this. At 35 and 42 d, the temperatures of the wing, head, shin, back, and cloaca in males and females were measured separately, and the average surface and body temperature were calculated. At 42 d, relative weights of the gizzard, liver, heart, and small intestine were calculated. The temperatures of the wings, back, and cloaca, and consequently the average surface temperature and body temperatures, were not affected by nutritional plans. Effects of increasing the nutritional and energy levels were observed on liver weights, the gizzard, and the small intestine. We conclude that the nutritional plans did not affect body temperature. Males had higher body temperatures than females. Body temperature increased with increase in age, and the increase in the nutritional plans increased liver weight and reduced the gizzard weights.

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Efektifitas Jamur Metharhizium anisopliae Menghambat Perkembangan Hama Uret pada Tanaman Cincau Hitam (Mesona palustris BL)

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v20i1.27 ◽

2019 ◽

Vol 20 (1) ◽

pp. 1-5

Author(s):

Wuye Ria Andayanie

Keyword(s):

Plant Growth ◽

The Body ◽

Diversity Analysis ◽

Root Volume ◽

Average Growth ◽

Straw Mulch ◽

Randomized Design ◽

Completely Randomized Design ◽

Average Plant ◽

Range Test

Black grass jelly plants can be used as conservation plants. However, uret pests (Leucopholis rorida F.) are often complained that eat the base of the stems, flowers and roots at night. This study aims to determine: 1) the effect of M. anisopliae fungi on uret pests, (2) the level of susceptibility of uret pests, and (3) M. anisopliae fungal effectiveness on uret pests. This study used a completely randomized design (CRD) with 4 treatments and 5 replications. Data from the observations were analyzed using analysis of variance (ANOVA) with Duncan's Multiple Range Test (DMRT) 5% test carried out when diversity analysis showed a real effect. Uret mortality monitoring will be carried out after the application of fungi. by observing each uret and calculating the amount of uret mortality and average plant growth. The highest percentage of second instar larvae from uret was found in treatment P4 (manure and fungi were incubated for 72 hours at the next temperature of 28 oC applied to the field with straw mulch) which was 82, 98% at 12 days after application of fungi. The average growth of plants consisting of: root length, root volume, stem diameter and number of shoots was also found in treatment P4 (manure and fungi were incubated for 72 hours at the next 28 oC, applied to the field with straw mulch), respectively amounting to 12.45; 5.70; 1.88; 12,15. Dead larvae emit fluids such as ethanol and their bodies are weak, then the body dries and hardens and stiffens, like mummies and fungi forming green sporulation. Keywords—: black grass jelly plants; Leucopholis rorida F;Metharhizium anisopliae; mortality;effectiveness

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Methionine plus cystine requirements for the maintenance and efficiency of utilization with tambaqui of different body weights

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044014920 ◽

2020 ◽

Vol 44 ◽

Author(s):

Neliane Galvão Porto ◽

Felipe Barbosa Ribeiro ◽

Jefferson Costa de Siqueira ◽

Marcos Antonio Delmondes Bomfim ◽

Rafael Silva Marchão ◽

...

Keyword(s):

Amino Acid ◽

Control Treatment ◽

Native Fish ◽

Amino Acid Requirement ◽

Treatment Groups ◽

Protein Retention ◽

Lack Of Information ◽

Randomized Design ◽

Body Weights ◽

Completely Randomized Design

ABSTRACT Methionine is an essential amino acid, and generally, the first limiting one in the diets of tambaqui, the main native fish produced in continental aquaculture in South America. However, there is a lack of information on their amino acid requirement, especially for maintenance and efficiency of utilization. The present study aimed to determine the requirement of [methionine plus cystine] for the maintenance and efficiency of utilization using tambaqui of different body weights. Two experiments were carried out using 250 tambaqui with weights of 100 and 300 g. They were distributed in a completely randomized design with four treatment groups (0.264, 0.396, 0.528, and 0.660% of digestible [Met + Cys]), five replications. The control treatment (CT) was performed with the addition of methionine at the first level (0.264%) until the second level was reached (0.396%). The maintenance requirement was determined by the regression analysis between the consumption of [Met + Cys] and protein retention equal to zero. The efficiency was determined using the value of the slope coefficient of the line obtained by the linear regression. The protein and methionine retention values of tambaqui weighing 100 to 300 g were compared by the parallelism test, and no difference was observed between the parameters of the equations indicating the need for only one equation to describe the responses to body weights. The requirement of digestible [Met + Cys] for the maintenance of tambaqui with weights from 100 to 300 g was 60.47 mg kg-0.7 day-1 and the efficiency of utilization of 42%.

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Karakteristik Kimia dan Organoleptik Flakes Kacang Hijau dengan Penambahan Bahan Pengisi dan Air

Jurnal Teknologi dan Industri Pertanian Indonesia ◽

10.17969/jtipi.v8i1.5251 ◽

2016 ◽

Vol 8 (1) ◽

pp. 24-28

Author(s):

Maya Indra Rasyid

Keyword(s):

Water Absorption ◽

Water Content ◽

Mung Bean ◽

Protein Levels ◽

Absorption Ratio ◽

Bean Flour ◽

Organoleptic Characteristics ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design

(Chemical and Organoleptic Characteristics of Mung Bean Flakes with The Addition of Fillers and Water) ABSTRACT. The objective of this research was to determine the chemical and organoleptic characteristics of mung bean flakes with the addition of fillers and water. The study was conducted using completely randomized design with two factors namely the addition of fillers and water. The results showed that the addition of fillers and water has significant (P≤0,01) effect on the water absorption ratio and protein levels of mung bean flakes. The best treatment was obtained by the mung bean flour without the addition of fillers with 150% of addition water with water absorption ratio of 204%, water content of 5,7%, protein of 22,01%, starch levels of 61,76%, organoleptic values of aroma of 2,61, color of 2,42, crispness of 2,72 and flavor of 2,83.

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Nutrient and inorganic solute (Na+ and Cl-) content in green onion plants under hydroponic cultivation using brackish water

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044013320 ◽

2020 ◽

Vol 44 ◽

Author(s):

Carlos Donato da Silva Souza ◽

Geronimo Ferreira da Silva ◽

Sirleide Maria de Menezes ◽

José Edson Florentino de Morais ◽

José Amilton Santos Júnior ◽

...

Keyword(s):

Nutrient Solution ◽

Brackish Water ◽

Hydroponic System ◽

Brackish Waters ◽

Randomized Design ◽

Hydroponic Cultivation ◽

Salinity Levels ◽

Completely Randomized Design ◽

Harmful Effects ◽

Different Levels

ABSTRACT Cultivation using brackish waters can result in nutritional and metabolic imbalances in several plant species, consequently reducing the production of dry matter (DM) and accumulation of toxic ions (Na+ and/or Cl-) in plants. We evaluated the DM production, and nutrient and inorganic solute (Na+ and Cl-) content in green onion plants (cv. Todo Ano Evergreen - Nebuka) under different levels of nutrient solution salinity in combination with circulation frequencies of this solution. Two experiments were conducted in a hydroponic system, using a completely randomized design, in a 6 × 2 factorial scheme, with five replicates: six levels of nutrient solution salinity (1.5, 3.0, 4.5, 6.0, 7.5, and 9.0 dS m-1) and two solution circulation frequencies (twice and thrice a day). In Experiment I, the evapotranspired depth was replaced using brackish water that was used to prepare each of the salinity levels (used exclusively), whereas in Experiment II, brackish water was used only to prepare each of the salinity levels and public water was used (electrical conductivity [ECw] = 0.12 dS m-1) for replacement in all treatments. The increase in the nutrient solution salinity reduced the production of DM and accumulation of nutrients; the reductions were more pronounced when brackish waters were used exclusively (Experiment I). However, the circulation of solutions thrice a day resulted in the harmful effects of the salinity effect. Replacing the evapotranspirated blade with water supply (Experiment II) mitigated the deleterious effects of salinity. Moreover, three circulations of the nutrient solution daily resulted in lower accumulation of inorganic Na+ and Cl- solutes and increased accumulation of nutrients N, P, K+, Ca2+, Mg2+, and S in the culture.

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The quality properties, thiobarbituric acid (TBA) values and microstructure of chicken sausage with local red beetroot powder

Food Research ◽

10.26656/fr.2017.5(s2).019 ◽

2021 ◽

Vol 5 (S2) ◽

pp. 113-119

Author(s):

W. Swastike ◽

E. Suryanto ◽

Rusman ◽

C. Hanim ◽

Jamhari ◽

...

Keyword(s):

Peroxide Value ◽

Room Temperature ◽

Thiobarbituric Acid ◽

Quality Properties ◽

Randomized Design ◽

Powder Addition ◽

Completely Randomized Design ◽

Different Levels ◽

Temperature Storage

This research was aimed to determine the quality properties, the microstructure of chicken sausage and Thiobarbituric acid (TBA) values with locally Indonesia red beetroot powder. The main ingredients of chicken sausage-making in this research were broiler chicken, filler, binder, beetroot powder, and spices. Red beetroot powder function as a filler was substituted tapioca starch in chicken sausage batter in three different levels. The combination of red beetroot powder with level 0, 1.0, 2.0 and 3.0% of total batter and shelf life at room temperature for 0, 1, 2 and 3 days. Each treatment consisted of five replications. The variables observed using quality properties (moisture, ash, fat, protein, crude fiber and calorie), microstructure and peroxide value of chicken sausage. The data of quality properties and peroxide value were analyzed by using one-way analysis (ANOVA) of Completely Randomized Design. The differences between means were analyzed by Duncan's New Multiple Ranges Test. The data of microstructure was analyzed by descriptive analyses. The moisture, protein, fat and ash contents for chicken sausages were significantly different (p<0.05). The chicken sausage with 2% substitution of beet powder produced chicken sausages with a high protein content of 14.77±0.02% while a low-fat content is 0.42±0.01%. Thiobarbituric acid (TBA) values of chicken sausages increased throughout the three days of room temperature storage (38°C). Chicken sausage formulated with red beetroot powder showed a significantly lower TBA value compared to the samples without red beetroot powder (p<0.05). In conclusion, a higher level of beetroot powder will improve the quality of chicken sausage and also the microstructure. The best level of beetroot powder addition was 2.0%. The addition of beetroot powder able to maintain fresh sausage conditions up to 2 days of storage at room temperature.

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