scholarly journals Preliminary evaluation of the antimicrobial activity of different Hermetia illucens larvae extracts for application as a cosmetic ingredient

2020 ◽  
Vol 17 (2) ◽  
pp. 1-10
Author(s):  
Cíntia Almeida ◽  
Daniel Murta ◽  
Rui Nunes ◽  
André Rolim Baby ◽  
Patrícia Rijo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lipid Fraction ◽  
Current Trend ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Preliminary Evaluation ◽  
Hermetia Illucens ◽  
Crude Extracts ◽  
High Lipid ◽  
High Concentrations

The cosmetic industry is continually searching for innovative ingredients, and there is a current trend in sourcing materials of natural origin. The Hermetia illucens (Linnaeus) larvae biomass is a potential source of compounds with applicability in cosmetics due to its high lipid, protein, and polysaccharide content. High concentrations of lauric acid, also known for also having antimicrobial properties, may be obtained from the lipid fraction. Additionally, these larvae are known to have a well-developed immune system, surviving in decomposition habitats, and can be induced to produce antimicrobial peptides (AMP's). These substances could be probed as alternative preservatives in cosmetics, as consumers are often concerned with the safety of conventional preservatives, such as parabens. The present study conducted a preliminary assessment of the antimicrobial activity of the crude extracts obtained from different extraction methods and extraction solvents against four microorganisms (Gram-positive and negative bacteria and yeasts). The use of diluted extracts did not show relevant inhibition of the growth of the tested microorganisms compared to the positive controls. Further assays are foreseen to test the antimicrobial activity of compounds or fractions isolated from the crude extracts.

scholarly journals Antioxidant, Antihypertensive and Antimicrobial Properties of Phenolic Compounds Obtained from Native Plants by Different Extraction Methods

2021 ◽  
Vol 18 (5) ◽  
pp. 2475
Author(s):  
Francisco Ramiro Boy ◽  
Rocío Casquete ◽  
Ana Martínez ◽  
María de Guía Córdoba ◽  
Santiago Ruíz-Moyano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Native Plants ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Ultra Sound ◽  
Antihypertensive Activity ◽  
Ultrasound Extraction ◽  
Rubus Ulmifolius ◽  
Cistus Salviifolius

This study aims to evaluate the efficacy of two methods (agitation and ultra-sound) for extracting phenolic compounds from 15 native plants. Plant species collected in the Dehesa of Extremadura were used. The antioxidant, antihypertensive and antimicrobial activity of the phenolic extracts was investigated. Significantly different results were obtained when comparing the two extraction methods, with the highest concentrations of phenolic compounds found for ultrasound extraction. In addition, the extracts obtained for Cistus albidus, Cistus salviifolius, Rubus ulmifolius and Quercus ilex showed the highest concentrations of phenolic compounds. The antioxidant activity was higher in the extracts of Cistus and Q. ilex obtained by ultrasound, as was the antihypertensive activity. Antimicrobial activity was also higher in the extracts obtained by ultrasound from C. salviifolius and Q. ilex plants against bacteria and from Cistus ladanifer against yeasts. Therefore, it can be concluded that, with the ultrasound extraction of phenolic compounds from C. ladanifer, C. albidus and Q. ilex plants, it is possible to obtain extracts with important functional properties, so they could be studied for their use in food with the aim of obtaining healthy and safe products, favouring the sustainability of the environment of the Dehesa Extremeña.

scholarly journals Evaluation of antimicrobial activity of three important medicinal plants of South India

2019 ◽  
Vol 8 (1) ◽  
pp. 3484
Author(s):  
Gopalakrishnaiah B. ◽  
Aniel Kumar O.
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Development Program ◽  
Antimicrobial Effect ◽  
Trichophyton Mentagrophytes ◽  
Crude Extracts ◽  
The World

India is found to be a country with rich biodiversity and enormous treasure of herbal plants and consequently called as medicinal garden of the world. Plants are the richest source of natural antimicrobial agents. In recent years drug resistance to human pathogenic bacteria has been commonly reported from all over the world. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases; one approach is to screen local medicinal plants for possible antimicrobial properties. The present study was designed to evaluate the antimicrobial efficacy of hexane, chloroform and methanol crude extracts of the leaves of three important medicinal plants viz., Biophytum sensitivum (L.) DC, Bougainvillea spectabilis L. and Caesalpinia bonducella (L.) Fleming, collected from in and around Visakhapatnam District. The antimicrobial activity of the crude extracts was tested against three Gram Positive bacteria (Bacillus subtilis MTCC 441, Enterococcus faecalis MTCC 439, Staphylococcus aureus MTCC 737), Three Gram Negative bacteria (Escherichia coli MTCC 443, Proteus vulgaris MTCC 426 and Pseudomonas aeruginosa MTCC 1688) and three Fungal strains (Candida albicans MTCC 227, Epidermophyton floccosum MTCC 613 and Trichophyton mentagrophytes MTCC 7687) using agar well diffusion assay. Our results demonstrated that methanol extracts of these plants leaves have concentration dependent antibacterial activity against some of the tested organisms. Further studies should be undertaken to elucidate the exact mechanism of action of antimicrobial effect to identify the active ingredients which can be used for drug development program.

scholarly journals Emergent Technologies for the Extraction of Antioxidants from Prickly Pear Peel and Their Antimicrobial Activity

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 570
Author(s):  
Elisabete M. C. Alexandre ◽  
Marta C. Coelho ◽  
Kardelen Ozcan ◽  
Carlos A. Pinto ◽  
José A. Teixeira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolics ◽  
Ohmic Heating ◽  
Antimicrobial Properties ◽  
Growth Curves ◽  
Extraction Methods ◽  
Prickly Pear ◽  
Emergent Technologies ◽  
Viable Cells ◽  
Thermal Extraction

Phenolic compounds are important bioactive compounds identified in prickly pear peel that have important antioxidant and antimicrobial properties. However, conventional thermal extraction methods may reduce their bioactivity, and technologies such as high pressure (HP) and ohmic heating (OH) may help preserve them. In this study, both technologies were analyzed, individually and combined (250/500 MPa; 40/70 °C; ethanol concentration 30/70%), and compared with Soxhlet with regard to total phenolics, flavonoids, and carotenoids as well as antioxidant (ABTS, DPPH, ORAC), DNA pro-oxidant, and antimicrobial (inhibition halos, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curves, and viable cells) activities of prickly pear peel extracts. Total phenolics extracted by each technology increased 103% (OH) and 98% (HP) with regard to Soxhlet, but the contents of total flavonoids and carotenoids were similar. Antioxidant activity increased with HP and OH (between 35% and 63%), and OH (70 °C) did not induce DNA degradation. The phenolic compound present in higher amounts was piscidic acid, followed by eucomic acid and citrate. In general, their extraction was significantly favored by HP and OH. Antimicrobial activity against 7 types of bacteria showed effective results only against S. aureus, S. enteritidis, and B. cereus. No synergetic or additive effect was observed for HP/OH.

scholarly journals Hemp Growth Factors and Extraction Methods Effect on Antimicrobial Activity of Hemp Seed Oil: A Systematic Review

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 183
Author(s):  
Klaudia Ostapczuk ◽  
Samuel Obeng Apori ◽  
Giovani Estrada ◽  
Furong Tian
Keyword(s):  
Antimicrobial Activity ◽  
Growth Factors ◽  
Seed Oil ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Hemp Seed ◽  
Research Attention ◽  
Research Fields ◽  
Seed Flour ◽  
Dpph Scavenging

The bioactive Hemp Seed Oil (HSO) is becoming very popular in the medical and research fields due to its antimicrobial properties against several diseases caused by bacteria and fungi. However, the effect of hemp-growing factors and extraction methods on the bioactivity of HSO does not receive adequate research attention. Therefore, this review aims to investigate the effect of growth factors and extraction methods on the antimicrobial activity of HSO. Articles were retrieved from Google Scholar and the Scopus database and screened against inclusion and exclusion criteria. The study revealed that HSO prefers warm climates and favorable humidity ranging from 20 to 39 °C and 79–100% per year, respectively, and rainfall of 324 mm daily. The multivariate linear regression shown excellent prediction (R2 = 0.94) with climates upon Zone of Growth Inhibition (ZGI) of Gram-positive bacteria. Temperature is the strongest predictor (p < 0.01) followed by humidity and rainfall (p < 0.05). Furthermore, well-drained loam soil rich in organic matter seems to stimulate the antimicrobial activity of HSO. The major constituents that influence HSO’s antimicrobial ability to Staphylococcus aureus were cannabidiol (CBD), β-caryophyllene, and limonene. The extraction methods showed less influence on the HSO bioactivity. HSO did not show significant antioxidant activity, but Hemp Seed Hull (HSH), Hemp Seed Flour (HSF), and Hydrolyzed Hemp Seed Protein (HPH), expressed promising DPPH scavenging ability.

scholarly journals The survival rate of virulent coagulase-negative staphylococci in pasteurized spiced kunu (a Nigerian non-alcoholic beverage)

Food Research ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 64-69
Author(s):  
Patience Fowoyo
Keyword(s):  
Antimicrobial Activity ◽  
Alcoholic Beverage ◽  
Antimicrobial Properties ◽  
High Concentration ◽  
Coagulase Negative Staphylococci ◽  
Gastrointestinal Infections ◽  
Antibiotic Resistant ◽  
High Concentrations ◽  
Rate Of Decline ◽  
Staphylococcus Simulans

Kunu is a non-alcoholic fermented beverage popularly consumed in Nigeria and attributed to possess immense nutritional and antimicrobial properties. The objective of this research was to determine the survival of virulent and antibiotic resistant coagulase-negative staphylococci (CoNS) in pasteurized spiced kunu. Kunu was prepared using the spices; clove and ginger and then pasteurized. The survival of five virulent and antibiotic resistant CoNS species; Staphylococcus simulans, Staphylococcus kloosi, Staphylococcus epidermidis, Staphylococcus caprae and Staphylococcus xylosus in pasteurized spiced kunu samples was determined at intervals of 0 hr, 24 hrs, 48 hrs, 72 hrs and 96 hrs. It was observed that there was a significant decrease of virulent and antibiotic resistant CoNS species in kunu samples prepared with high concentrations of the spice either alone or in combination. The virulent strains were below detectable limit from 48 hrs especially for those prepared with a combination of spices at high concentration and that with clove alone. The un-spiced kunu (control) also inhibited the virulent species although the rate of decline was not as pronounced like the spiced kunu even at 96 hrs. This study had shown that kunu prepared with a combination of clove and ginger at high concentration of 30 mg/ mL exhibits antimicrobial activity against CoNS. Kunu may be used as an antimicrobial against gastrointestinal infections caused by these organisms. Further studies may be carried out using other gastrointestinal pathogens to fully explore and lend credence to the antimicrobial activity of spiced kunu.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

ANTIMICROBIAL ACTIVITY OF THREE INSECT SPECIES, CRUDE EXTRACTS AGAINTS CERTAIN MICROBIAL AGENTS.

2015 ◽  
Vol 26 (Issue 2-C) ◽  
pp. 19-24
Author(s):  
MOSTAFA HASSAN ◽  
ALY MOHAMED ◽  
MONEER AMER ◽  
KOTB HAMMAD ◽  
MAHMOUD MAHBOUB
Keyword(s):  
Antimicrobial Activity ◽  
Insect Species ◽  
Crude Extracts ◽  
Microbial Agents

Altered composition of high-lipid diet may generate reactive oxygen species by disturbing the balance of antioxidant and free radicals

2020 ◽  
Vol 31 (3) ◽  
Author(s):  
Arnab Banerjee ◽  
Debasmita Das ◽  
Rajarshi Paul ◽  
Sandipan Roy ◽  
Ankita Bhattacharjee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Reactive Oxygen ◽  
Control Group ◽  
Preliminary Evaluation ◽  
Oxygen Species ◽  
High Lipid ◽  
Tnf Α ◽  
High Lipid Diet ◽  
Lipid Diet

AbstractBackgroundIn the present era, obesity is increasing rapidly, and high dietary intake of lipid could be a noteworthy risk factor for the occasion of obesity, as well as nonalcoholic fatty liver disease, which is the independent risk factor for type 2 diabetes and cardiovascular disease. For a long time, high-lipid diet (HLD) in “fast food” is turning into part of our everyday life. So, we were interested in fulfilling the paucity of studies by means of preliminary evaluation of these three alternative doses of HLD on a rat model and elucidating the possible mechanism of these effects and divulging the most alarming dose.MethodsThirty-two rats were taken, and of these, 24 were fed with HLD in three distinctive compositions of edible coconut oil and vanaspati ghee in a ratio of 2:3, 3:2 and 1:1 (n = 8), orally through gavage at a dose of 10 mL/kg body weight for a period of 28 days, whereas the other eight were selected to comprise the control group.ResultsAfter completion of the experiment, followed by analysis of data it was revealed that hyperlipidemia with increased liver and cardiac marker enzymes, are associated with hepatocellular injury and cardiac damage. The data also supported increased proinflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α). As oxidative stress parameter increased in both liver and heart, there is also an increased in TNF-α due to an increased expression of inducible nitric oxide (NO) synthase, which led to a high production of NO. Moreover, HLD treatment explicitly weakens reasonability of hepatocytes and cardiomyocytes conceivably through G0/G1 or S stage capture or perhaps by means of enlistment of sub-G0/G1 DNA fragmentation and a sign of apoptosis.ConclusionsBased on the outcomes, it tends to be inferred that consequences of the present examination uncovered HLD in combination of 2:3 applies most encouraging systemic damage by reactive oxygen species generation and hyperlipidemia and necroapoptosis of the liver and heart. Hence, outcome of this study may help to formulate health care strategy and warns about the food habit in universal population regarding the use of hydrogenated and saturated fats (vanaspati ghee) in diet.

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