scholarly journals Comparison of the Effectiveness of Tomato (Lycopersicum commune) and Red Melon (Cucumis melo L.) Juice in Increasing Vaginal Wall Thickness of Menopausal Rats Model

2019 ◽  
Vol 55 (3) ◽  
pp. 159
Author(s):  
Yhenti Widjayanti ◽  
Arief Widya Prasetya
Keyword(s):  
Wall Thickness ◽  
Vaginal Wall ◽  
Menopausal Symptoms ◽  
Tomato Juice ◽  
Control Group ◽  
Ovarian Activity ◽  
Treatment Groups ◽  
Significant Difference ◽  
Vegetables And Fruits ◽  
Vaginal Wall Thickness

Menopausal period is the period of permanent cessation of menstruation due to reduced or lost ovarian activity. One of the effects of decline estrogen levels during menopause is atropic vulvovaginitis. Consumption of phytoestrogens can reduce menopausal symptoms. Phytoestrogen is found in vegetables and fruits such as tomatoes and red melon. The objective of this study was to compare the effectiveness of red melon juice and tomato on the increase of vaginal wall thickness in menopausal rats. This study revealed an alternative of phytoestrogen sources that is more effective to reduce menopausal symptoms, especially those related to vaginal atrophy to improve the quality of life of menopausal women. Subjects of this study were 27 ovariectomized Wistar strain Rattus norvegicus which were s divided into 3 groups randomly, one control group and two treatment groups with tomato juice and red melon juice at a dose of 330 mg/kgBW/day administered for 28 days. The variable of this study were the thickness of the vaginal wall which was precipitated by Hematoxiline Eosine. The highest vaginal wall thickness was found in the treatment group of red melon juice of 879.89 ± 70.52 µm and the lowest of the control group (K) of 643.3 ± 58.33 µm. The results of Anova test showed that there was a significant difference between control group and treatment groups, the tomato juice and red melon juice with a dose of 330 mg/kgBW/day, so it can be concluded that red melon juice with a dose of 330mg/Kg BW/day has the best effect in increasing the thickness of vaginal wall of menopausal rats as compared with tomato juice.

scholarly journals The Effect of Tomato Juice in Increasing Ki-67 Expression and Epithelial Thickness on The Vaginal Wall of Menopausal Rats

2019 ◽  
Vol 11 (2) ◽  
pp. 152-8
Author(s):  
Juminten Saimin ◽  
Hendy Hendarto ◽  
Soetjipto Soetjipto
Keyword(s):  
Cell Proliferation ◽  
Vaginal Wall ◽  
Tomato Juice ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Distilled Water ◽  
Ki 67 ◽  
Treatment Groups ◽  
Epithelial Thickness ◽  
Significant Difference

BACKGROUND: Dyspareunia and pain due to the decrease of vaginal wall thickness usually happen in menopausal women. The reduction of estrogen levels cause the decreasing of cell proliferation and the thinning of vaginal wall epithelium. Tomato (Solanum lycopersicum) is a source of phytoestrogens, which produce estrogenic effects. This study aims to assess the effect of tomato juice on Ki-67 expression and epithelial thickness of the vaginal wall in menopausal rats.METHODS: This was an experimental study using Sprague-Dawley rats. Twenty-four female rats, aged 4 months and weighing 150-200 grams, were divided into 4 groups. Each group consisted of 6 rats. Negative control (NC) group was group of rats with sham procedure and performed by distilled water for 28 days. Positive control (PC) group was group of bilateral ovariectomized rats and performed by distilled water for 28 days. The first treatment (T1) group was group of bilateral ovariectomized rats, given tomato juice at dose of 11g/200g body weight (BW)/day. The second treatment (T2) group was group of bilateral ovariectomized rats, given tomato juice at dose of 15g/200g BW/day. Data analysis was done with Anova, multiple comparisons and regression test.RESULTS: The group with lowest Ki-67 expression was PC group (2.52±0.60). The expression of Ki-67 in treatment groups (T1 and T2) was higher than PC group, but lower than NC group. There was no significant difference between groups (p=0.771). The lowest epithelial thickness was found in PC group (21.19±3.96) and the highest was found in the treatment groups (38.73±12.43). There was positive correlation between tomato juice and epithelial thickness (p=0.647).CONCLUSION: Tomato juice increases Ki-67 expression and epithelial thickness on the vaginal wall of menopausal rats. The increase of epithelial thickness follows the administration dose, but Ki-67 expression does not exceed in the control rats. Tomato juice increases the cell proliferation of vaginal wall on menopausal rats, however the increase is still within normal limits.KEYWORDS: epithelial thickness, Ki-67, menopause, tomato juice

Capsular closure versus unrepaired interportal capsulotomy after hip arthroscopy in patients with femoroacetabular impingement, results of a patient-blinded randomised controlled trial

2021 ◽  
pp. 112070002110057
Author(s):  
Niels H Bech ◽  
Inger N Sierevelt ◽  
Sheryl de Waard ◽  
Boudijn S H Joling ◽  
Gino M M J Kerkhoffs ◽  
...  
Keyword(s):  
Randomised Controlled Trial ◽  
Femoroacetabular Impingement ◽  
Hip Arthroscopy ◽  
Controlled Trial ◽  
Poor Quality ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Randomised Controlled

Background: Hip capsular management after hip arthroscopy remains a topic of debate. Most available current literature is of poor quality and are retrospective or cohort studies. As of today, no clear consensus exists on capsular management after hip arthroscopy. Purpose: To evaluate the effect of routine capsular closure versus unrepaired capsulotomy after interportal capsulotomy measured with NRS pain and the Copenhagen Hip and Groin Outcome Score (HAGOS). Materials and methods: All eligible patients with femoroacetabular impingement who opt for hip arthroscopy ( n = 116) were randomly assigned to one of both treatment groups and were operated by a single surgeon. Postoperative pain was measured with the NRS score weekly the first 12 weeks after surgery. The HAGOS questionnaire was measured at 12 and 52 weeks postoperatively. Results: Baseline characteristics and operation details were comparable between treatment groups. Regarding the NRS pain no significant difference was found between groups at any point the first 12 weeks after surgery ( p = 0.67). Both groups significantly improved after surgery ( p < 0.001). After 3 months follow-up there were no differences between groups for the HAGOS questionnaire except for the domain sport ( p = 0.02) in favour of the control group. After 12 months follow-up there were no differences between both treatment groups on all HAGOS domains ( p  > 0.05). Conclusions: The results of this randomised controlled trial show highest possible evidence that there is no reason for routinely capsular closure after interportal capsulotomy at the end of hip arthroscopy. Trial Registration: This trial was registered at the CCMO Dutch Trial Register: NL55669.048.15.

scholarly journals SUBCHRONIC TOXICITY TEST OF COMBINATION ETHANOL EXTRACT OF DETAM 1 SOYBEAN (GLYCINE MAX L. MERR) AND JATI BELANDA (GUAZUMA ULMIFOLIA LAMK) TOWARD FUNCTION, WEIGHT, AND HISTOPATHOLOGICAL OF WISTAR RAT LIVER

2016 ◽  
Vol 9 (5) ◽  
pp. 197
Author(s):  
Meilinah Hidayat ◽  
Sijani Prahastuti ◽  
Estherolita Dewi ◽  
Dewi Safitri ◽  
Siti Farah Rahmawati ◽  
...  
Keyword(s):  
Liver Function ◽  
Toxicity Test ◽  
Low Dose ◽  
Ethanol Extract ◽  
Good Effect ◽  
Control Group ◽  
High Dose ◽  
Subchronic Toxicity ◽  
Treatment Groups ◽  
Significant Difference

ABSTRACTObjective: As an antiobesity therapy, combination extracts of Detam 1 soybean and Jati Belanda will be consumed for a long time; therefore, theirtoxicities to the liver need to be investigated. To determine the effect of subchronic toxicity test of combination of ethanol extract of Detam 1 soybean(EEDS) and ethanol extract of Jati Belanda (EEJB) on liver function with parameters: Alanine transaminase (ALT), macroscopic, and histopathologicalof liver.Methods: This study was conducted on 120 Wistar rats (60 males and 60 females), 90 days (treatment group) and 120 days (satellite group). Ratswere divided into six treatment groups (3 test materials, 1 control, and 2 satellites); each group included 10 males and 10 females.Results: ALT levels of treatment groups (low dose, medium, and high), both males and females were lower than the control group (p<0.05). Thetreatment groups demonstrated a good effects effect on liver function. Liver weight of all groups showed no significant difference compared with thecontrol group (p>0.05). Results of histopathological score interpretation of male and female liver rats of low dose groups were not disturbed; middledose groups were slightly disturbed and high dose groups were damaged. Satellite high doses of male groups were disrupted, while female groupswere not.Conclusion: The combination of EEDS and EEJB has a good effect on liver function, did not lead to change organ weight and at low doses did not causerenal histopathology damage in rats after 90 days administration.Keywords: Combination of soybean Jati Belanda, Toxicity subchronic test, Function, Weight, Histopathology, Liver.

scholarly journals OP13.05: Ultrasound measurement of vaginal wall thickness is a novel and reliable technique

2009 ◽  
Vol 34 (S1) ◽  
pp. 103-103
Author(s):  
D. C. Panayi ◽  
R. Fernando ◽  
P. Tekkis ◽  
G. A. Digesu ◽  
V. Khullar
Keyword(s):  
Wall Thickness ◽  
Vaginal Wall ◽  
Ultrasound Measurement ◽  
Reliable Technique ◽  
Vaginal Wall Thickness

A prospective randomized study to assess the efficacy of post-operative nasal medication after endonasal surgery

1995 ◽  
Vol 109 (7) ◽  
pp. 618-621 ◽  
Author(s):  
Paul D. R. Spraggs ◽  
Marcelle Macnamara ◽  
Theo Joseph
Keyword(s):  
Randomized Study ◽  
Correlation Coefficients ◽  
Control Group ◽  
Time Intervals ◽  
Treatment Groups ◽  
Ephedrine Hydrochloride ◽  
Patent Airway ◽  
Significant Difference ◽  
Blood Clots ◽  
Betamethasone Sodium Phosphate

AbstractPost-operative nasal medications are commonly used following routine septal or turbinate surgery but their efficacy in removing blood clots, improving the sensation of a patent airway and promoting healing are unknown. This prospective randomized trial of patients undergoing septal and/or turbinate surgery assessed the efficacy of three commonly used nasal medicines, 0.5 per cent ephedrine hydrochloride nasal drops, betamethasone sodium phosphate (Betnosol®) nose drops and alkaline nasal douches, in producing the sensation of a patent airway in the 14 days following surgery. Ninety-seven patients were randomized into the three treatment groups and a control group who received no nasal medication. Patients assessed their nasal patency by means of a visual analogue scale (VAS) and any complications of treatment were recorded. Statistical analysis of the 76 complete sets of results using the Mann-Whitney U-test showed that there was a significant difference in the distribution of all of the treatments for each of the time intervals (p<0.05). Glass rank biserial correlation coefficients were all small (rg<0.085) but the most significant differences were between ephedrine and the control group at two hours, two, seven and 10 days (0.02, 0.054, 0.057, 0.085 respectively), alkaline nasal douches being most significant at four and 14 days (0.06 and 0.0722 respectively).

Particular Matter of Motor Vehicle Exposure and High-Fat Diet Effects on Kidney Histopathology, Creatinine, and Malondialdehyde (MDA) Levels in Wistar Rats

2021 ◽  
Vol 5 (3) ◽  
pp. 124-128
Author(s):  
Rizka Veni ◽  
Awal Prasetyo ◽  
Muflihatul Muniroh
Keyword(s):  
High Fat Diet ◽  
Wistar Rats ◽  
Motor Vehicle ◽  
Histopathological Change ◽  
Normal Diet ◽  
Control Group ◽  
High Fat ◽  
Control Group Design ◽  
Treatment Groups ◽  
Significant Difference

This study aims to analyze the effect of combination of motor vehicle particular matter exposure and high-fat diet in kidney histopathology, creatinine levels, and MDA levels in Wistar rats. This study used a posttest-only control group design. Eighteen healthy male Wistar rats were divided into three groups. The intervention groups received motor vehicle fume exposure for 100 s with normal diet (X1) or high-fat diet (X2), and the control group received no exposure (C). Data analysis was processed with a SPSS 25.0 computer program by using the one-way ANOVA test followed by post hoc LSD. The degree of kidney histopathological damage showed significant differences between the X1 and X2 groups when compared with the control group (p < 0.05). The results of the creatinine level examination found a significant difference between the X2 and C groups (p < 0.05) and the treatment groups X1 and X2 (p < 0.05). The results of kidney MDA level examination showed a significant difference between the treatment groups (X1 and X2) and the control group (p < 0.05). The combination of particular matter of motor vehicle fumes exposure and high-fat diet could induce kidney damage through histopathological change and increased creatinine levels and kidney MDA levels in Wistar rats.

scholarly journals Coenzyme q10 is potentially usedto improve lipid profile in diabetic hypercholesterolemia-induced periodontitis in the coastal area

2018 ◽  
Vol 47 ◽  
pp. 07006
Author(s):  
Saryono Saryono ◽  
Hesti Devinta ◽  
Abdul Haris Budi Widodo ◽  
Arif Imam Hidayat
Keyword(s):  
Lipid Profile ◽  
Coenzyme Q10 ◽  
Coastal Area ◽  
Positive Control ◽  
Atherogenic Index ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Pap Method ◽  
Post Hoc

Contaminated water often affects the occurrence of periodontitis in the coastal area. The diabetic hypercholesterolemia-induced periodontitis in Indonesia is very high. The use of coenzyme Q10 to treat this disease has never been investigated yet. Therefore, this study aimedto analyze the effect of coenzyme Q10 on the lipid profile of diabetic hypercholesterolemia-induced periodontitis.Twenty four rats were randomized into 6 groups (G1-G6). The groups (G1-G3) are healthy, negative and positive control group respectively. The treatment groups (G4-G6) are diabetic hypercholesterolemia-induced rats given coenzyme q10 dose of 13,5; 27 and 54 mg/kg respectively. Rats were induced by periodontitis, hypercholesterolemia and diabetes mellitus. Coenzyme Q10 was administered orally using 2 mL gastric tube once a day for 14 days. Lipid profile including triglycerides, HDL, and atherogenic index (IA) was measured enzymatically by the CHOD-PAP method. Data were analyzed by one-way ANOVA test and followed by the Least Significant Difference (LSD) post hoc test.Coenzyme Q10 with a dose of 54 mg/kgis effective in lowering triglyceride, and atherogenic index and increasing HDL level in diabetic hyperlipidemia-induced periodontitis rats model. This research supports the potential effects of coenzyme Q10 supplementation to improve lipid profile in diabetic hypercholesterolemia-induced periodontitis in the coastal area.

scholarly journals The Effects of Mycophenolate on the Formation of Granulation Tissue Post-operatively in Canine Tracheal Stent Patients (2014–2020)

2021 ◽  
Vol 8 ◽  
Author(s):  
Kevin F. Barber ◽  
Catherine A. Loughin ◽  
Dominic J. Marino ◽  
Martin Lesser
Keyword(s):  
Mycophenolate Mofetil ◽  
Granulation Tissue ◽  
Control Group ◽  
Tracheal Lumen ◽  
Loss To Follow Up ◽  
Treatment Groups ◽  
Tracheal Stent ◽  
Significant Difference ◽  
Tracheal Collapse

Objectives: To determine if mycophenolate mofetil reduces the incidence and severity of granulation tissue in-growth in canine tracheal stent patients.Study design: Randomized clinical trial.Animals: 111 dogs from the hospital population.Methods: Client-owned dogs that received an endoluminal self-expanding tracheal stent for canine tracheal collapse between 2014 and 2020 were randomly assigned into one of two treatment groups. Control group medication protocol consisted of prednisone 0.5 mg/kg PO BID/SID/EOD × 30 days, hydrocodone 0.25 mg/kg PO TID × 30 days, and cefovecin 8 mg/kg SQ post-placement. Mycophenolate group medication protocol was identical to the control group medication protocol with the addition of mycophenolate mofetil 10 mg/kg PO BID × 30 days, SID for life. Recheck tracheoscopy was performed at 1, 3, and 6 months post-stent placement. Presence and severity of granulation tissue were determined by tracheoscopy and were recorded as a percentage of tracheal lumen obstruction by blinded evaluators (none present, &lt;25%, &gt;25–50%, and &gt;50%).Results: At none of the three time points was there a statistically significant difference in grade between controls and those receiving mycophenolate (p = 0.467, p = 0.330, and p = 0.410).Conclusions and Clinical Significance: Our results suggest that mycophenolate can be safely given to these patients but do not support that its administration will reduce the incidence and severity of granulation tissue. Although a difference was observed in the severity of granulation tissue between the two groups, loss to follow-up may have influenced conclusions. A larger study would be warranted to further evaluate the effect of mycophenolate on the development of granulation tissue.

scholarly journals EFEK PEMBERIAN JUST TOMAT (LYCOPERSICUM PYRIPORME) TERHADAP SPERMATOGENESIS PADA TIKUS PUTIH (RATTUS NORVERGICUS) JANTAN DEWASA HYPERKHOLESTROLEMIA

2011 ◽  
Vol 35 (1) ◽  
pp. 27 ◽  
Author(s):  
Eliza Anas ◽  
Asterina Asterina
Keyword(s):  
Enzyme Activity ◽  
Risk Factor ◽  
Adult Male ◽  
Control Design ◽  
Not Given ◽  
Treatment Groups ◽  
Significant Difference ◽  
Randomized Control ◽  
Vegetables And Fruits ◽  
Coa Reductase

AbstrakHiperkolesterolemia merupakan faktor resiko penyebab kematian di usia muda. Salah satu terapi yang sekarang dikembangkan adalah dengan banyak mengkonsurnsi sayur dan buah-buahan. Tomat mengandung zat-zat gizi antara lain likopen. Likopen memegang peranan penting didalam pengaturan kolesterol, yaitu dengan menghambat kerja enzim HMG-CoA reduktase, yang berperan dalam proses sintesis kolesterol sehingga berefek hipokolesterolemia. Sedangkan hormone testoteron dibentuk dari kolesterol. Tujuan penelitian ini ingin melihat efek pemberian jus tomat (Lycopersicum pyriporme) kukus terhadap spermatogenesis tikus (Rattus norvegikus) putih jantan hiperkolesterolemia.Desain penelitian adalah eksperimental dengan rancangan pretest and postest randomized control design, Sampel berjurnlah 24 ekor tikus hiperkolesterol, dilakukan pada 4 kelompok dimana 3 kelompok perlakuan diberikan jus tomat kukus selama lebih kurang 14 hari, sedangkan pada kelompok kontrol tidak diberikan just tomat kukus, hanya diberikan aquades.Dari hasil penelitian ditemukan bahwa pada pemberian dosis 2ml/200gr BB dan 2,5ml/200gr BB tidak ada perbedaan yang bermakna terhadap jumlah spermatogonium bila dibandingkan dengan kontrol sedangkan pada dosis 3ml/200gr BB ada perbedaan yang bemakna (P<0,05) terhadap jumlah spermatogonium dibandingkan dengan kontrol. Pada jumlah spermatosit pakiten, spermatid dan spermatozoa pada ke tiga dosis memperlihatkan perbedaan yang sangat bermakna (P.<0,01) bila dibandingkan dengan kontrol.Dari hasil yang didapatkan dapat disimpulkan, dengan pemberian jus tomat kukus dapat memperbaiki spermatogenesis tikus yang hiperkolesterolemia.Kata Kunci : Hiperkolesterolemia, Lycopersicum pyriporme, spermatogenesisAbstractHypercholestrolemia are the cause risk factor of the death in young ages. One of therapy current develop are many consuming vegetables and fruits. Tomatoes contain nutrient including lycopene. Lycopenes have important roleARTIKEL PENELITIAN28in regulation of cholesterol, which is inhibit enzyme activity, HMG-CoA reductase, which serve in process of synthesis so that have effect hypocholesterolemia, whereas testosterone is processed from cholesterol. The aim to this study is to show effect of giving the steam of tomato juices (Lyeopersieum pyripome) to spermatogenesis in adult male white mice (Rattus novergicus) with hypercholesterolemia.The design of study is experimental with pretest design and posted randomized contro design. 24 mice with cholesterolemia is performed to 4 groups, where three treatment groups are given the steam of tomato juices for abot 14 days, whereas the control is not given , but given aquadest only.Of the result is found that in giving both doses 2 ml/200 g b.w. and 2,5 ml/200 g b.w. is not significant difference to number of spermatogoium rather than control, whereas at dosage 3 ml/g b.w. there are significant difference to number of spermatogonium (13 <0,05), rather than control. In number of spermatocyte pachyten, spermatid and spermatozoa in all three doses show significant difference (P<0,01) rather than control. From the result we can be concluded that giving the steam of tomato juices can improve spermatogenesis in mice with hypercholesterolemia.Key word : Hypercholesterolemia, Lycopersicum pyriporme, spermatogenesis

scholarly journals The Effect of Aloe Vera Extract to Extensive Lesion and Expression of Transforming Growth Factor (TGF-β) on Alkaline Chemical Trauma Cornea Model

2018 ◽  
Vol 1 (2) ◽  
pp. 1-17
Author(s):  
Fahcreza ◽  
Elsa Iskandar ◽  
Rachmat Hidayat ◽  
Petty Purwanita ◽  
Anang Tribowo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wistar Rats ◽  
Aloe Vera ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Significant Difference ◽  
The Difference ◽  
In Cells

Abstract Background: Chemical trauma to the cornea is an emergency condition of the eye that requires early diagnosis and good treatment. Alkaline have ability to saponify fatty acids in cells and cell membranes which can make penetration into the stroma and destroy proteoglycans and collagen in cells. Aloe vera (AV) contains several active substances that are reported to have anti-inflammatory, immunomodulatory, and wound healing effects. AV has been reported to accelerate the healing process of corneal epithelial defects by increasing fibroblast proliferation, collagen production and growth factor production. This study aims to determine the difference between the effect of aloe vera extract with a concentration of 10%, 20%, 40% and BSS on the healing of extensive corneal lesions in white wistar rats alkaline trauma models. Method: This study was an experimental study with a pre and posttest only with control group design in vivo approach to 30 Wistar white rats which were divided into 5 treatment groups for 3 days. Comparative analysis of effectiveness using the ANNOVA test or the Kruskal Wallis test and continued by the post hoc test. Results: Based on the one way ANOVA test there was a statistically significant difference in effectiveness between the five treatment groups on the percentage of corneal wound healing area and TGF-β expression with an assessment of p = 0,000 each. The administration of alloevera (AV) concentration of 20% had a significant difference in percentage of healing of corneal lesions and TGF-β expression compared with other treatment groups with p = 0,000 each. Large differences in the area of corneal lesions in the 40% AV group were -0.45 in the BBS group, 0.146 in the 10% AV group, 0.493 in the 20% AV group. The difference in the AV group 10% was 0.30 in the BBS group, -064 in the AV group 20%, and -0.14 in the AV group 40%. However, TGFβ expression in the normal control group that did not receive treatment was 54.94 (53.21-56-12). TGFβ levels in the BSS group were 10.44, the 10% aloe vera group was 25.43, 47.99 for the 20% aloe vera group and 37.95 for the 40% aloe vera group. Conclusion: There is a difference between the effect of aloe vera extract with concentrations of 10%, 20%, 40% and BSS on the extensive healing of corneal lesions in white wistar rats with alkaline chemical trauma models.

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