scholarly journals Pengaruh Penggunaan Larutan Daun Bandotan (Ageratum conyzoides) Terhadap Kadar Glukosa Darah Ikan Koi (Cyprinus carpio) Pasca Transportasi [Influence Use Of Bandotan (Ageratum conyzoides) To Rate Koi Fish (Cyprinus carpio) Blood Glucose After Transportation]

2019 ◽  
Vol 5 (1) ◽  
pp. 73
Author(s):  
Laksmi Sulmartiwi, Sri Harweni, Akhmad Taufiq Mukti, Rr. Juni Triastuti
Keyword(s):  
Water Quality ◽  
Blood Glucose ◽  
Cyprinus Carpio ◽  
Research Result ◽  
Experimental Treatment ◽  
Blocking Effect ◽  
Fish Mortality ◽  
Ageratum Conyzoides ◽  
Transport Medium ◽  
Fish Blood

Abstract Transportation makes stress for fish because of high density and decreasing of water quality. Stress can increase blood glucose. Bandotan have Ca blocking effect. The Ca blocking effect occur in reticulum endoplasmic in smooth muscle. This research aimed to identify bandotan leaf effect in transport medium on blood glucose of koi fish post transportation and to identify best dosage of bandotan leaf on koi fish tranport medium to decrease the blood glucose of koi fish post transportation. Research method used was experimental. Treatment used was providign different bandotan leaf dossage: A (0 g/L), B (0.45 g/L), C (0,9 g/L), D (1.35 g/L) and E (1.8 g/L) and each treatment was repeaetd four times. Main parameter observed in this research was koi fish blood glucose every eight hours for twenty four hours and supporting parameter consisted of: water quality and fish mortality rate. Then the data obtained were prepared using ANOVA, continued by Dunnett Test and then by Duncan’s Multiple Distance. Research result shwoed that bandotan leaf supply in transport medium had no effect on koi fish blood glucose post transportation and highest dosage of bandotan leaf in transport medium on blood glucose of koi fish post transportation was 0.45 g/L.

scholarly journals Bioaktivitas Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Terhadap Respon Fisiologis (Glukosa Darah Dan Tachiventilasi) Ikan Koi (Cyprinus carpio) [Bioactivity Essential Oil Of Bandotan Leaf (Ageratum conyzoides) On Koi (Cyprinus carpio) Physiological Responses (Blood Glucose And Tachiventilation) ]

2019 ◽  
Vol 6 (1) ◽  
pp. 81
Author(s):  
Laksmi Sulmartiwi, Mardiah Rahma Umami, Moch. Amin Alamsjah, Sri Subekti
Keyword(s):  
Blood Glucose ◽  
Essential Oil ◽  
Cyprinus Carpio ◽  
Inhibitory Effect ◽  
Early Observation ◽  
Test Results ◽  
Ageratum Conyzoides ◽  
Randomized Design ◽  
Reduce Blood Glucose ◽  
Range Test

Abstract Koi (C. carpio) is one of famous ornamental fish in Indonesia. Fish often be subject of change or stessor like physical, chemical and biology on culture. Stress in fish caused released cortisol hormone and cathecolamine hormone. Cathecolamine increase glicogenolisis, cardiovascular regulation and respiration fungtion. Blood glucose and tachiventilation (opercular movement) can indicate stress respon in fish. Essential oil of A. conyzoides have central analgesic activity. Analgesic drugs in vertebrae can reduce stress in fish. Essential oil shown inhibitory effect of GABA transminase, an enzime GABA (gammaaminobutryic acid) degradation which can provoking sedation. Sedation effect in fish reduce blood glucose and tachiventilation. Potency bioactive essential oil of bandotan leaf compound haven’t been know in aquaculture. The potency based of LC50 (Lethal Consentration 50 %) value Purpose of this research determined effect of essential oil Ageratum conyzoides leaf on Koi (Cyprinus carpio) Phsycology Response (Blood Glucose and Tachiventilation) every 4 hours durung 24 hours and LC50-24 hours. Research was conducted at Laboratory of Faculty of Fisheries and Marine Airlangga University Surabaya. Research design used Completely Randomized Design and followed by Duncan's Multiple range test. Results of analysis of variance (ANAVA) from early observation and every 4 hours during 24 hours day showed that each treatment of essential oil Ageratum conyzoides leaf gave a significantly different effect on the blood glucose and tachiventilation (P <0.05). The highest average of blood glucose was found in D treatment at second observation (443,67 mg/dl) and the lowest was found in C treatment (60,67 mg/dl). The highest average of tachiventilation was found in D treatment at first observation (1730 bit/9 minute) and the lowest was found in A treatment (437,67 bit/9 minute) at early observation. LC50-24 hours essenial oil Ageratum conyzoides leaf is 34,047 ppm. Based on measurements of water quality, treatments was still viable and good to supported viability of Cyprinus carpio during research, water temperature range between 29-31oC, range of dissolved oxigen 4-6 mg/l, pH ranges 7,5-8,3 and 0,0060,27 mg/l on ammonia.

scholarly journals Potensi Sedasi Minyak Atsiri Daun Bandotan (Ageratum conyzoides) terhadap Ikan Koi (Cyprinus carpio) [Sedation Potential Essential Oil of Bandotan Leaf (Ageratum conyzoides) to Koi Fish (Cyprinus carpio)]

2017 ◽  
Vol 9 (2) ◽  
pp. 107
Author(s):  
Ade Wahyu Pratama ◽  
Laksmi Sulmartiwi ◽  
Boedi Setya Rahardja
Keyword(s):  
Water Quality ◽  
Low Temperature ◽  
Essential Oil ◽  
Cyprinus Carpio ◽  
Recovery Time ◽  
Time Span ◽  
Ageratum Conyzoides ◽  
Analgesic Drugs ◽  
Time In Treatment ◽  
Leaf Essential Oil

                                                              AbstrakTransportasi ikan berhubungan dengan metode pembiusan. Pembiusan ini dilakukan untuk menurunkan metabolisme atau keaktifan (sedative). Metabolisme yang tinggi selama transportasi dapat diminimalkan dengan menggunakan metode imotilisasi. Dengan suhu atau senyawa metabolik, bahan antimetabolik alami yang dapat digunakan adalah tanaman bandotan (Ageratum conyzoides). Bandotan merupakan tanaman obat yang mengandung minyak atsiri dan saponin (Kardono, 2003). Pada bidang perikanan, untuk mengurangi stres pada ikan dan juga digunakan menenangkan serta penurunan keaktifan (sedative) atau obat analgesik yang digunakan pada hewan vertebrata (Neiffer and Stamper, 2009). Tujuan dari penelitian ini untuk mengetahui potensi sedasi pemberian minyak atsiri daun bandotan terhadap ikan koi (Cyprinus carpio). Hasil analisis varian (ANAVA) menunjukkan bahwa setiap perlakuan minyak atsiri daun bandotan memberikan pengaruh terhadap waktu pingsan, waktu pulih sadar dan tachiventilasi (p<0,05). Rentang waktu pingsan tercepat pada perlakuan I saat menit ke- 27-30 dan rentang waktu pingsan terlama pada perlakuan D saat menit ke- 55. Rentang waktu pulih sadar tercepat pada perlakuan D saat menit ke- 5 dan rentang waktu pulih sadar terlama pada perlakuan I saat menit ke- 15-18. Rata-rata tachiventilasi tertinggi pada perlakuan A pada pengamatan menit ke- 60 (810 bit/5 menit) dan terendah pada I (343 bit/5 menit) pada pengamatan menit ke- 60. Berdasarkan pengukuran kualitas air yaitu suhu air antara 29-31o C, oksigen terlarut 6 ppm, pH berkisar 8-8,3 dan amoniak 0,02-0,27 mg/l.                                                                  AbstractTransportation of fish associated with the method of anesthesia. Anesthesia is done to decrease metabolism or liveliness (sedative). A high metabolism during transport can be minimized by using imotilisasi. Imotilisasi can be done by using a low temperature or chemical or natural metabolic compounds (Soedibya and Pramono, 2006). Antimetabolic natural ingredients that can be used is a plant bandotan (Ageratum conyzoides). Bandotan is a medicinal plant that contains essential oils and saponins (Kardono, 2003). In the field of fisheries, to reduce stress on the fish and also used a calming as well as a decrease in the activity (sedative) or analgesic drugs used in vertebrate animals (Neiffer and Stamper, 2009). The purpose of this study to determine the potential for sedation administration bandotan leaf essential oil to the koi (Cyprinus carpio). Results of analysis of variance (ANOVA) showed that each treatment bandotan leaf essential oil to give effect to the time unconscious, conscious and tachiventilasi recovery time (p<0.05). Range fastest time in treatment I fainted while 27-30 minutes and the longest time span in treatment D fainted while 55 minute span of the fastest recovering conscious at the time of treatment D 5 minutes and recovered aware longest time span in treatment I while 15-18 minutes. The highest average tachiventilasi on treatment A on the 60 minute observation (810 bits / 5 min) and the lowest in the I (343 bits / 5 min) on minute 60 observation. Based on the measurement of water quality is the water temperature between 29-31o C, dissolved oxygen is 6 ppm, pH ranged from 8 to 8.3 and ammonia 0.02 to 0.27 mg / l

scholarly journals Pengaruh Pemberian Minyak Atsiri Daun Bandotan (Ageratum conyzoides) Dalam Transportasi Tertutup Benih Ikan Koi (Cyprinus Carpio) [The Effects Of Bandotan Leave’s (Ageratum conyzoides) Essential Oil within Closed System Transportation of Koi Carp (Cyprinus carpio) ]

2019 ◽  
Vol 6 (2) ◽  
pp. 215
Author(s):  
Laksmi Sulmartiwi, Ida Bagus Putu Oka, Rr. Juni Triastuti
Keyword(s):  
Blood Glucose ◽  
Survival Rate ◽  
Essential Oils ◽  
Cyprinus Carpio ◽  
Closed System ◽  
Ornamental Fish ◽  
Ageratum Conyzoides ◽  
Koi Carp ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract In the ornamental fish business, transportation has an important role to distributing fish up into the costumers. The distance between the farm and the customers location, require a transportation method which can maintain the quality and quantity of ornamental fish during the transportation. The biggest challenge in live fish closed system transportation is to reduce the stress level of fish during the process. According to Harmon (2009), fish are often exposed to multiple stressor during the transportation. Stressed fish can be seen through the increasing of tachiventilation (rapid opercula movement) and blood glucose levels (Evans and Claiborne, 2006). One of the method to reduce stress of fish during transportation is using essential oils from Bandotan’s (A. conyzoides) leaves. A. conyzoides essential oils know has an analgesic activity (Okunade, 2002; Shekhar and Anju, 2012; Singh et al., 2013). A chemical compound with known analgesic effect, used in human and veterinary medicine as local analgesic dan be used to fish for the purpose of sedation, immobilization, and analgesia as well as general anaesthesia (Zahl and Samuelsen, 2012). This research was conducted to see the effect of A.conyzoides essential oils toward the survival rate, blood glucose levels, and tachyventilation of koi carp juveniles during closed system transportation. This research using completely randomized design method which consist of 5 different treatments with 4 replications. Treatment that used in this research is the variation of administration doses of A. conyzoides essential oils during the koi carp juvenile closed system transportation. The treatment such as A (5 ppm), B (10 ppm), C (15 ppm), D (20 ppm), and E (0 ppm). Main parameter of this research is survival rate, blood glucose levels, and tachyventilation of koi carp juvenile during 8 hours transportation. The result shows that A. conyzoides essential oils give a significant result toward survival rate, blood glucose levels, and tachiventilation of koi carp juvenile during transportation. There is no mortality in treatment A, B, and E, during transportation. The lowest blood glucose levels and tachiventilation found in treatment A (5 ppm).

scholarly journals Effects of Different Fish Diets on the Water Quality in Semi-Intensive Common Carp (Cyprinus carpio) Farming

Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1215
Author(s):  
László Berzi-Nagy ◽  
Attila Mozsár ◽  
Flórián Tóth ◽  
Dénes Gál ◽  
Zoltán Nagy ◽  
...  
Keyword(s):  
Water Quality ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Nutrient Loading ◽  
Fish Meal ◽  
Pond Water ◽  
Long Term Effects ◽  
Plant Oil ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Semi-intensive common carp (Cyprinus carpio) farm technology uses several feed types affecting the growth performance; however, we know less about their long-term effects on water quality. Herein, we evaluated the effects of three commonly used feeds—moderate levels of fish meal and fish oil feed (FF), plant meal and plant oil feed (PF), and cereal feed (CF) on the nutrient (total nitrogen (TN), total phosphorus (TP) and organic matter (OM)) content of the pond water. The experiment was carried out over three consecutive years from juveniles to market-sized fish. The type of feed affected the net yields, but generally, it did not affect the water quality. The year of sampling, however, was a significant factor affecting TN, TP, and OM, whose concentrations decreased during the three years. Our findings highlight that the age of the stocked fish on water quality has a more pronounced effect than the nutrient profile of the supplementary feed. Additionally, the plant-based feed could provide comparable net yields as the fish meal-based feed without additional nutrient loading in the water column, reinforcing the sustainability of alternative feeds in semi-intensive carp farming.

DETERMINATION OF GLUCOSE IN FISH BLOOD: A MODIFICATION OF THE FOLIN–MALMROS MICROPROCEDURE

1958 ◽  
Vol 36 (11) ◽  
pp. 1121-1124 ◽  
Author(s):  
Leonard R. Murrell ◽  
Paul F. Nace
Keyword(s):  
Blood Glucose ◽  
Prussian Blue ◽  
Tungstic Acid ◽  
Sodium Lauryl Sulphate ◽  
Blood Proteins ◽  
Colorimetric Measurement ◽  
Efficient Separation ◽  
Fish Blood

A modification of the Folin–Malmros micromethod is described for the determination of fish blood glucose. A more efficient separation of blood proteins is obtained by precipitating 0.1 ml blood in 10.0 ml tungstic acid. This is followed by hot alkaline reduction of ferricyanide, and colorimetric measurement of a Prussian blue – sodium lauryl sulphate suspension. The method, which eliminates certain difficulties found in other procedures, has been successfully applied in more than a thousand analyses of blood from two species of fish (Opsanus and Ictalurus) having glucose concentrations between 15 and 250 mg per cent.

scholarly journals Identification of Pathogenic Bacteria in Cyprinus carpio Reared in Floating Cages

2021 ◽  
Vol 26 (1) ◽  
pp. 26
Author(s):  
Erikson Sahala Pardamean ◽  
Henni Syawal ◽  
Morina Riauwaty
Keyword(s):  
Water Quality ◽  
Cyprinus Carpio ◽  
Pathogenic Bacteria ◽  
Survey Method ◽  
Fish Parasite ◽  
Pseudomonas Sp ◽  
Fish Sample ◽  
Purposive Sampling ◽  
Fish Samples ◽  
Cultured Fish

Pathogenic bacteria is disease causing microorganisms that can attack fish and can cause mass death in cultured fish. The purpose of this study was to identify the types of pathogenic bacteria Cyprinus carpio that were reared in floating cages. This study used a survey method that is purposive sampling and the fish sample were obtained from, PLTA Koto Panjang. the fishes were identified in the Fish Parasite and Disease Laboratory, Fish and Marine Faculty, University of Riau. Fish samples used were 15-20 cm goldfish totaling 12 tails with 3 times taken from 4 different cages. The kidneys organ were examined and to find out the type the bacteria. Result shown identified of pathogenic bacteria consist of 3 types of bacteria namely Aeromonas sp., Pseudomonas sp., and Edwardsiella sp. Water quality during the study was Temperature: 27-300C, pH 6-7, DO 3.67-4.28 ppm, and ammonia 0.048-0.64 mg/L.

scholarly journals Budidaya ikan mas (Cyprinus carpio) sistem akuaponik dengan padat penebaran berbeda

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Darwis Darwis ◽  
Joppy D. Mudeng ◽  
Sammy N.J. Londong
Keyword(s):  
Water Quality ◽  
Cyprinus Carpio ◽  
Stocking Density ◽  
Quality Parameters ◽  
Water Volume ◽  
Daily Growth ◽  
Growth And Survival ◽  
Experimental Fish ◽  
The Difference

This research aimed to determine the stocking density that support the best growth and survival rate of carp cultivated in aquaponic systems, and to study the water quality of carp culture with different stocking densities in aquaponic systems. The study used 12 aquaria measuring 40x40x40 cm each (water volume of 50 liters). The experimental fish are carp (Cyprinus carpio) weighing in average of 3.5 g/individual.  The fish were cultivated with different stocking densities as treatment, including A = 4; B = 7; C = 10 and D = 13 individuals/aquarium.  The fish were fed with pellet at 5%/body weight/day with a feeding frequency of two times a day. The study was conducted for 4 weeks. Data consisting of daily growth, survival and water quality parameters (temperature, pH, dissolved oxygen, ammonia, nitrite and nitrate) were measured once a week. The data obtained were analyzed by ANOVA. The results of the study showed that the difference in stocking density had no significant effect on the growth and survival of carp.  Water quality was in a reasonable range to support the growth and survival of carp cultivated with aquaponic systems.Keywords: cultivation, carp, aquaponics, stocking density, growth

scholarly journals Design of Monitoring System for Salt Concentration in Milkfish Ponds using Android

2019 ◽  
Vol 9 (2) ◽  
pp. 42-46
Author(s):  
Nuzlya Ramadhana
Keyword(s):  
Water Quality ◽  
Water Content ◽  
Monitoring System ◽  
Salt Concentration ◽  
Sea Water ◽  
Salt Content ◽  
Temperature Sensors ◽  
Fish Mortality ◽  
Measuring Instruments ◽  
Ph Sensors

In the cultivation of milkfish, water quality is very concerned about in order to minimize fish mortality when cultivated. Several important factors in the cultivation of milkfish are the level of salt content in the water, temperature and pH. Currently, farmers monitor water quality manually by coming directly to see water quality. If there are dead fish, the cultivator will replace the new water. This will have an impact on fish production which will decrease. The milkfish pond uses brackish water for pond waters. The salt content is between the sea water content and the fresh water content. The grade level may change from season to season. This change is due to biological processes that occur in these waters as well as the interaction between pond waters and the surrounding environment. The results showed that the results of the design of the salt concentration monitoring system in the milkfish ponds that have been carried out using Arduino Uno, conductivity sensors, pH sensors, temperature sensors, wifi modules, Mi-Fi and Smartphones to access android applications. The results of testing the microcontroller functionality that have been made are in accordance with the design and are running well. The system designed to transmit information in the form of salinity values, pH sensors, temperature sensors and TDS values ??with sensor accuracy that is linear with measurement results using conventional measuring instruments with the highest error values ??of 1.3%, 2.06%, 0.702% and 1.5 respectively. %.

scholarly journals The effects of differences in water exchange on the productivity of glass eel stage in Anguilla bicolor bicolor culture

2021 ◽  
Vol 322 ◽  
pp. 02006
Author(s):  
Eri Setiadi ◽  
Adang Saputra ◽  
Imam Taufik
Keyword(s):  
Water Quality ◽  
Blood Glucose ◽  
Survival Rate ◽  
Quality Management ◽  
Water Exchange ◽  
Water Quality Management ◽  
Economic Value ◽  
Culture Technique ◽  
Glass Eel ◽  
Survival And Growth

Eel, Anguilla bicolor bicolor has an economic value for export commodity. Eel farming has been developed both of home to industries scales. However, the main problem during the glass eel culture is the low survival rate, characterized by high mortality. This condition is related to the culture technique that has not been established yet, especially water quality management. The research aims to determine the best water exchange during the culture of the glass eel on the survival, growth, and blood glucose. The different percentages of water exchange as a treatment were followed A) control (without water exchange); B) 20% of water exchange; C) 40% of water exchange; and D) 60% of water exchange. The water exchange has affected the survival, growth, and blood glucose (P<0.05). The ammonia, nitrite, and nitrate values during the culture period showed that the control was higher compared to the others. The water exchange of 40% is suitable for glass eel stage culture to obtain the best survival and growth.

scholarly journals Designing a Mobile Application for Pool Water Quality Monitoring Based on the Internet of Things

2021 ◽  
Vol 9 (1) ◽  
pp. 47-55
Author(s):  
Yohanes Anton Nugroho ◽  
Muhammad Fitra Pratama
Keyword(s):  
Water Quality ◽  
Internet Of Things ◽  
Water Quality Monitoring ◽  
Quality Monitoring ◽  
Fish Mortality ◽  
The Internet ◽  
Monitoring Equipment ◽  
Pool Water ◽  
Internet Of Things Technology ◽  
The Internet Of Things

Changes in temperature, pH, and turbidity in concrete fish ponds greatly impact to the fish survival. Initial observations showed that among 3.067 fish seeds, 1.633 fish (53%) died and only 1.434 fish (47%) was successfully harvested. The application of water quality monitoring devices from concrete pools designed based on the Internet of Things technology has been tested. The monitoring equipment will not function optimally without an application that functions to receive monitoring data and then take action. Pool water quality monitoring equipment connected to the cloud using a GSM network connection. The recorded data is then displayed on the water quality monitoring application that designed using the Android operating system. Application design is developed using a User-Centered Design approach, where the design process was carried out by considering several variables: ease for use, clarity of information delivery, the fulfillment of needs, and appearance. Based on the results of the design evaluation, weaknesses can be determined, namely, difficulty to find the search menu for click history data, find the refresh button, read the results of searching for historical data, and read data in tables and graphs. Based on this, further improvements can be made to improve the application being made. The monitoring equipment is expected to provide information to pond managers to immediately take action if changing in pH and temperature beyond the limit so that the fish mortality rate can be minimized.

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