scholarly journals Inhibition Activity of Garlic (Allium sativum) Skin Aqueous Extract on Mastitis Causing Microorganisms

2020 ◽  
Vol 21 (1) ◽  
pp. 38
Author(s):  
Afduha Nurus Syamsi ◽  
Meyta Pratiwi ◽  
Aras Prasetiyo Nugroho
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
Design Research ◽  
Inhibition Activity ◽  
Animal Science ◽  
Minimum Concentration ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Streptococcus Mutants

Mastitis in dairy caused by microorganisms, e.g. Staphylococcus aureus and Candida albicans, is a serious problem due to its effect in decreasing quality and quantity of milk production up to 53.5%. Mastitis precautions are generally carried out using iodine solution and has risk leaving residue both on the udder and milk produced. Natural antimicrobial compounds need to be developed to solve iodine residues. An alternative natural ingredient that can be used is the skin of garlic which is known contains allicin, that is able to inhibit the growth of pathogenic microorganisms. This completely randomized design research by applying Kirby-Bauer method, aimed to identify the effect and concentration of garlic skin extracts that are effective against the growth of Staphylococcus aureus, Streptococcus mutants, Escherichia coli and Candida albicans. Research was done in 6 months in Faculty of Animal Science and Faculty of Biology Unsoed, and the datas were analyzed using ANOVA. Garlic skins were extracted using aquadest to three concentrations i.e. 5%, 10% and 15%, and were tested on to mastitis-causing microorganisms growth. The results showed the extract of garlic skins using aquadest has high potency in inhibiting the growth of mastitis-causing microorganisms. Based on the results, aqueous extract of garlic skin has inhibition activity with minimum concentration 5-10%.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae)

2020 ◽  
Vol 151 ◽  
pp. 15550-15558
Author(s):  
Amégninou Agban ◽  
Yao Hoekou ◽  
Passimna Pissang ◽  
Tchadjobo Tchacondo ◽  
Komlan Batawila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
E Coli ◽  
Toxicological Studies ◽  
Jatropha Multifida

Objectif : L’objectif de ce travail était d’évaluer in vitro l’activité antimicrobienne des extraits de feuilles et tige de Jatropha multifida sur la croissance de Candida albicans, Escherichia coli et Staphylococcus aureus, puis d’évaluer in vivo la toxicité de cette plante. Méthodologie et résultats : Les méthodes de diffusion en milieu gélosé et de microdilution en milieu liquide ont été utilisées pour évaluer l’effet antimicrobien. Une étude en subaigüe était réalisée afin d’explorer les effets toxiques de l’extrait aqueux des feuilles. Les résultats des tests antimicrobiens montrent une activité des extraits de feuilles et tige de J. multifida sur la croissance des souches utilisées avec des diamètres de zones d’inhibition allant de 8 à 25 mm et des concentrations minimales inhibitrices (CMI) variant de 0,039 mg/mL à 1,25 mg/mL à l’exception des souches de E. coli qui sont résistantes aux extraits de la tige. L’administration en subaigüe de l’extrait aqueux des feuilles de J. multifida à la dose de 600 mg/kg entraîne une perte significative de poids chez les souris. Conclusion et applications des résultats : Les extraits aqueux, éthanolique et hydroéthanolique des feuilles et tige de J. multifida possèdent d’activité antimicrobienne et pourraient être utilisés dans le traitement des Candidoses à C. albicans et des infections à S. aureus. Mais l’essai de toxicité subaigüe montre que l’extrait aqueux de la plante serait toxique. Des études toxicologiques approfondies restent donc nécessaires sur ces extraits afin de mieux élucider leur inocuité. Mots-clés : Jatropha multifida, extraits de feuilles et de tige, activités antifongique et antibactérienne, toxicité. Agban et al., J. Appl. Biosci. 2020 Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae) 15551 Evaluation of antimicrobial potential and toxicity of Jatropha multifida Linn, (Euphorbiaceae) extracts ABSTRACT Objective: The objective of this study was to evaluate in vitro the antimicrobial activity of leaves and stem of Jatropha multifida extracts against Candida albicans, Escherichia coli and Staphylococcus aureus, and then to evaluate in vivo the toxicity of this plant. Methodology and Results: The agar well-diffusion and the NCCLS broth microdilution methods were used to assess the antimicrobial effect. A subacute study was carried out to explore the toxic effects of the aqueous extract of the leaves. The results of the antimicrobial tests show an activity of the extracts of leaves and stems of J. multifida on the growth of the strains used with diameters of inhibitory zones ranging from 8 to 25 mm and minimum inhibitory concentrations (MIC) varying from 0.039 mg/mL to 1.25 mg/mL exception E. coli strains which are resistant to extracts from the stem. Subacute administration of the aqueous extract of the leaves of J. multifida at a dose of 600 mg/kg leads to a significant loss of weight in the mice. Conclusion and application of findings : The aqueous, ethanolic and hydroethanolic extracts of the leaves and stem of J. multifida have antimicrobial activity and could be used in the treatment of Candidiasis and bacterial infections due respectively to C. albicans and S. aureus. But the subacute toxicity test shows that the aqueous extract of the plant would be toxic. Extensive toxicological studies therefore remain necessary on these extracts in order to better elucidate their safety. Keywords: Jatropha multifida extracts of leaves and stem, antifungal and antibacterial activities, toxicity

scholarly journals UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC

2017 ◽  
Vol 5 (2) ◽  
pp. 47 ◽  
Author(s):  
Kadek Sudarmi ◽  
Ida Bagus Gede Darmayasa ◽  
I Ketut Muksin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Syzygium Cumini ◽  
E Coli ◽  
Randomized Design ◽  
Test Concentration ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Test Level

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins.   Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC  

scholarly journals PERCENTAGES OF CARCASS PARTS OF MALE LOCAL DUCKS FED TORCH GINGER FLOWER (Etlingera elatior) SOLUTION INCLUDED IN COMMERCIAL RATION

2018 ◽  
Vol 3 (2) ◽  
pp. 65
Author(s):  
Dwiki Tri Satya Wahyan ◽  
Ristika Handarini ◽  
Dede Kardaya
Keyword(s):  
Live Weight ◽  
Test Results ◽  
Breast Meat ◽  
Initial Body Weight ◽  
Science Study ◽  
Animal Science ◽  
Study Program ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Etlingera Elatior

Breast and thigh are the parts of duck carcass which are most commonly utilized and consumed by people.  These carcass parts consist of meat, skin, and bones.  This study was aimed at assessing the effects of the inclusion of torch ginger flower solution in commercial ration on live weight and percentages of carcass and its parts of male local ducks.  The study was conducted from June to August 2016 at Poultry Laboratory of Animal Science Study Program, Faculty of Agriculture, Djuanda University, Bogor.  Twenty-four male local ducks aged 2 weeks with average initial body weight of 450 ± 53.04 g were used.  A completely randomized design with 4 treatments and 3 replicates was used.  Treatments consisted of commercial ration + 0% torch ginger flower solution (R0), commercial ration + 2.5% torch ginger flower solution (R1), commercial ration + 5.0% torch ginger flower solution (R2), and commercial ration + 7.5% torch ginger flower solution (R3).  Measurements were taken on the percentages of whole breast, breast meat, breast bones, whole thigh, thigh meat, and thigh bones.  Data were subjected to an analysis of variance and a Duncan test.  Results showed that treatments gave significant effect (P<0.05) on the percentage of thigh meat.  The inclusion of torch ginger flower solution in ration did not negatively affect carcass parts of male local ducks.  The inclusion of 7.5% torch ginger flower solution in commercial ration increased the percentage of whole breast.  It was also found that the inclusion of 5.0 and 7.5% torch ginger flower solution in commercial ration increased the percentage of thigh meat.Key words: torch ginger flower solution, male local duck, carcass parts

scholarly journals Antimicrobial and antifungal activity of new fluorophenyl-containing 1,2,4-triazoles

2021 ◽  
pp. 87-93
Author(s):  
О. А. Бігдан
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Practical Significance ◽  
Scientific Publications ◽  
Reference Drug ◽  
Minimum Concentration ◽  
Antimicrobial And Antifungal Activity ◽  
Derivatives Of ◽  
Serial Dilutions

The achievements of the world organic chemistry are most clearly represented by scientific publications that prove the promising nature of heterocyclic substances. The possibility of combining various pharmacophore fragments and 1,2,4-triazole in one molecule is quite popular. In 2018, the drug Trifuzol-NEO appeared on the veterinary market of Ukraine, which took its rightful place among synthetic immunomodulators for various groups of unproductive animals. So, further studies of new promising compounds among substituted 1,2,4-triazole, which can be used as objects for the creation of new original domestic antimicrobial and antifungal agents, remains relevant and has theoretical and practical significance. The aim of our work was to investigate the antimicrobial and antifungal activity of a number of new fluorophenyl-containing derivatives of 1,2,4-triazole and, in some cases, to trace the presence of certain patterns between structure and action. The sensitivity of new fluorophenyl-containing derivatives of 1,2.4-triazole was studied by the method of serial dilutions in accordance with the methodological recommendations. From the initial concentration of the new synthesized compounds of 1 mg/ml, a series of two-fold serial dilutions were prepared in Mueller–Hinton broth in a volume of 1 ml. Then, 0.1 ml of microbial curtain (106 m. c./ml) was added to each tube. MIC (MIC) was determined in the absence of visible growth in a test tube with the minimum concentration of the drug, the minimum bactericidal concentration (MBcK) – in the absence of growth on agar after inoculation from transparent tubes. Dimethyl sulfoxide was used as a solvent for the compounds in the studies. The research was carried out at the Department of Microbiology, Virology and Immunology of Zaporizhzhia State Medical University. Analyzing the results of studying the sensitivity of substances to Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, it should be noted that almost all compounds were active against the bacteria. It should be noted that a number of compounds were found to be the most active against Staphylococcus aureus, and 5-(2-fluorophenyl)-4-(((5-nitrofuran-2-yl)methylene)amino-4H-1,2,4-triazole-3-thiols generally exceeded several times the activity indices of the reference drug (MIC 1.95 μg/ml, MBcK 3.9 μg/ml) to Staphylococcus aureus. Most of the compounds were found to be quite active against Candida albicans. Among the corresponding 5-(2-fluorophenyl)-4-((aryl)ylidene)amino-1,2,4-triazole-3-thiols, the highest activity for Candida albicans exhibit 5-(2-fluorophenyl) -4-((4-bromophenyl)ylidene)amino-1,2,4-triazole-3-thiol and 5-(2-fluorophenyl)-4-((2,3-dimethoxyphenyl)ylidene)amino-1,2,4-triazole-3-thiol. It was found that most of the compounds exhibit a moderate antimicrobial and a fairly high antifungal effect. The most sensitive strain was S. aureus in relation to 5-(2-fluorophenyl)-4-(((5-nitrofurans-2-yl)methylene)amino-4H-1,2,4-triazole-3-thiol, Candida albicans proved to be very sensitive to 5-(2-fluorophenyl)-4-((4-bromophenyl)ylidene)amino-1,2,4-triazole-3-thiol and 5-(2-fluorophenyl)-4((2,3-dimethoxyphenyl)ylidene)amino-1,2,4-triazole-3-thiol.

scholarly journals The Potential Use of EDTA as an Alternative to Defibrination in Preparing Blood Agar Plates with Human AB Blood Type on Staphylococcus aureus Culture

2021 ◽  
Vol 3 (1) ◽  
pp. 64-71
Author(s):  
Dora Dayu Rahma Turista ◽  
Eka Puspitasari ◽  
Fanny Kurnanda Razvi
Keyword(s):  
Staphylococcus Aureus ◽  
Ethylenediaminetetraacetic Acid ◽  
Blood Type ◽  
Blood Agar ◽  
Clotting Factors ◽  
Randomized Design ◽  
Blood Clots ◽  
Completely Randomized Design ◽  
Potential Use ◽  
Blood Clotting Factors

Blood Agar Plates (BAP) are composed of blood as one of the compositions. Sheep’s blood is usually used, but since it is difficult to be obtained, human AB blood type was used as an alternative. In preparing BAP, blood is defibrinated to lyse the blood clotting factors. Blood clots can also be prevented by adding anticoagulants, such as ethylenediaminetetraacetic acid (EDTA). This study aims to investigate the potential use of EDTA as a substitute for defibrination in preparing BAP with human AB blood type. This study employed a completely randomized design with true experimental method using Staphylococcus aureus as the sample. The parameters were the number of colonies, types of hemolysis, and hemolysis zone. The results showed that the S. aureus grown on BAP with EDTA-human AB blood type was 64 colonies (mean), produced β-hemolytic pattern, and 6 mm hemolytic zone. In contrast, the S. aureus grown on BAP with defibrinated human AB blood type showed 82 colonies (mean), β-hemolytic pattern, and 5 mm hemolytic zone. There were significant differences in the number of colonies (0.000 < α) and hemolytic zones (0.02 < α). However, there was no difference in the hemolysis type (both treatments produced β-hemolysis). EDTA was possible to be used as a substitute for defibrination in preparing BAP to assess the hemolysis type of S. aureus, but it might not be able to be used as a benchmark for counting the number of colonies and determining the hemolysis zone of S. aureus.

scholarly journals Antimicrobial Activity of Methanolic and Aqueous Extract of Rhodomyrtus tomentosa Leaves against Staphylococcus aureus and Escherichia coli

2021 ◽  
Vol 15 (1) ◽  
pp. 186-193
Author(s):  
Sarah Sukeri ◽  
Azizah Ab Karem ◽  
Evana Kamarudin ◽  
Mazura Bahari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Statistical Analysis ◽  
Aqueous Extract ◽  
Food Preparation ◽  
P Value ◽  
Sensitivity Testing ◽  
Minimum Concentration ◽  
Significant Difference ◽  
Rhodomyrtus Tomentosa

Food poisoning is one of the riskiest illnesses caused by an organism like bacteria, viruses and parasites, which can contaminate food at any stage of food production. Most reports are associated with bacterial contamination through the food preparation process. Researcher nowadays attempts to discover a safer agent to use in food preparation as a food preservative agent. Thus, the potential of Rhodomyrtus tomentosa leaves as an antimicrobial agent was explored in this study. It was tested against two bacteria which are Staphylococcus aureus (gram-positive) and Eschericia coli (gram-negative). Antimicrobial activity of methanol and aqueous R. tomentosa leaves extract was compared using gram staining, bacterial identification, antimicrobial sensitivity testing (AST), minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and statistical analysis. The disc diffusion technique was used for AST where the mean area of inhibition methanol and aqueous extract against S. aureus was 18 mm and 16 mm respectively, whereas there was no area of inhibition for E. coli. MIC was done to determine the minimum concentration of the extract needed to inhibit the bacteria. After that, MBC was performed to confirm the previous MIC result. Statistical analysis was done by conduct it through SPSS software program. Then, the result for S. aureus is obtained from finding its p-value. Statistically, a significant difference between the two bacteria was analyzed by using independent t-test. A result of p-value less than 0.05 was considered significantly different as one another. Statistical analysis showed no significant difference between methanol and aqueous extract on antimicrobial activity. In conclusion, both aqueous and methanol leaves R. tomentosa yielded similar antibacterial activity which susceptible to S. aureus and can be used safely toward foodborne pathogen.

scholarly journals Studi Invitro Daun Alpukat (Persea americana Mill.) Untuk Pengobatan Sariawan

2019 ◽  
Author(s):  
Rizki Rizki ◽  
Yelsi Sepri Melda ◽  
Mades Fifendy
Keyword(s):  
Candida Albicans ◽  
Saturated Fat ◽  
Nutrient Content ◽  
Persea Americana ◽  
Randomized Design ◽  
Avocado Fruit ◽  
High Nutrient ◽  
Completely Randomized Design ◽  
Test Level ◽  
Very High

Avocado (Persea americana Mill.) efficacious for treating various diseases. Avocado fruit has a very high nutrient content. Fruit contains 11 vitamins and 14 minerals that are beneficial. Avocados are rich in protein, riboflavin, niacin, potassium, essential oils, such as methylchavikol, alphapinene, tannins, and flavonoids. The pulp contains saturated fat, protein, sesqueterpenes, vitamin A, B1, and B2, and the leaves contain active compounds flavonoids, tannins, and quersetin. Candida albicans is a fungus that can cause various diseases, one sprue. Research has been conducted in order to determine the inhibition of leaf infusion avocado (Persea americana Mill.) on the growth of Candida albicans. This research was using a completely randomized design (CRD) with 6 treatments and 4 replications at each concentration infusion was 10%, 20%, 30%, 40%, 50%. Analysis of the data showed that treatment with each test level α f 5% significant and continued with HSD test. The results showed that 10% infusa avocado leaves (Persea americana Mill.) is able to inhibit the growth of Candida albicans.

scholarly journals Peningkatan Kualitas flakes Ganyong (canna edulis ker.) dan Bekatul Menggunakan Variasi Sayuran

2014 ◽  
Vol 18 (2) ◽  
Author(s):  
Haryo Sukamdani ◽  
L.M. Ekawati Purwijantiningsih ◽  
F. Sinung Pranata
Keyword(s):  
Staphylococcus Aureus ◽  
Raw Material ◽  
Plate Count ◽  
Soluble Fiber ◽  
Randomized Design ◽  
Cereal Product ◽  
Completely Randomized Design ◽  
And Staphylococcus Aureus ◽  
Insoluble Fiber

AbstractFlakes are ready to eat cereal product in order to provide a high caloric needs for those who consume them. Utilization of ganyong into flakes is one alternative that increases the diversification of processed ganyong prospects in the community. In order to improve the nutritional quality of flakes, especially fiber, other ingredients are added for flakes. Variety of vegetables and rice brand’s is used as the main raw material of flakes. Variety of vegetables is broccoli, spinach and carrots and with the addition of vegetables can be seen the influence of increasing the nutritional value from the resulting flakes. This research used completely randomized design (CRD). The results obtained in this study of flakes product are have variations of water content 0.03-0.36%, ash 2.69-3:09%, protein 5.72-6.36%, 0.96-3.08% fat, carbohydrate 87.47-90.61%, soluble fiber 1.59-4.4%, insoluble fiber 0.01-0.02%, total fiber 1.6-4.42%, texture 3349.8-3687.8 gf and microbiological tests such total plate count, molds and yeasts and Staphylococcus aureus that meets the ISO standard of cereal (SNI 01-4270 -1996). The results showed that the carrot flakes had the highest quality overall and flakes broccoli had the highest fiber content.Key words: Flakes, ganyong, variety of vegetables, rice bran’sAbstrakFlakes adalah produk makanan siap saji untuk menyediakan kalori bagi yang mengkonsumsinya. Pemanfaatan ganyong sebagai produk sereal adalah salah satu cara meningkatkan diversifikasi pangan di masyarakat. Dalam rangka meningkatkan kualitas gizi flakes, terutama serat maka bahan lain ditambahkan dalam pembuatan flakes. Variasi sayuran dan bekatul digunakan sebagai bahan baku utama pembuatan flakes. Variasi sayuran yang digunakan yakni brokoli, bayam dan wortel. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL). Hasil yang didapatkan dari penelitian ini yakni kadar air flakes 0,03-0,36%, kadar abu 2,69-3,09%, protein 5,72-6,36%, lemak 0,96-3,08%, karbohidrat 87,47-90,61%, serat larut 1,59-4,4%, serat tidak larut 0,01-0,02%, total serat 1,6-4,42%, tingkat kekerasan/ tekstur 3349,8-3687,8 gf dan tes mikrobiologi seperti angka lempeng total, kapang dan khamir, serta Staphylococcus aureus sesuai standar ISO sereal (SNI 01-4270 -1996). Hasil penelitian menunjukan bahwa flakes wortel mempunyai kualitas gizi tertinggi dan flakes brokoli mengandung serat tertinggi.Kata kunci: Flakes, ganyong, variasi sayuran, bekatul

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK LAMUN (Cymodocea rotundata) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli (Antibacterial Activities of Seagrass Extracts (Cymodocea rotundata) Against Staphylococcus aureus and Escherichia coli)

2017 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
Septiani Septiani ◽  
Eko Nurcahya Dewi ◽  
Ima Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
E Coli ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Cymodocea Rotundata

Cymodocea rotundata  merupakan salah satu jenis lamun yang berpotensi sebagai antibakteri. Senyawa bioaktif yang bersifat sebagai antibakteri adalah fenol, flavonoid dan tanin. Tujuan penelitian ini adalah untuk mengetahui perbedaan lama inkubasi dan konsentrasi ekstrak lamun C. rotundata yang berbeda terhadap aktivitas antibakteri S. aureus dan E. Coli. Metode penelitian yang digunakan yaitu experimental laboratories dengan menggunakan rancangan dasar penelitian Rancangan Acak Lengkap (RAL) dengan pola faktorial, pola terbagi oleh faktor lama inkubasi bakteri (24 jam, 48 jam dan 72 jam) dan perbedaan konsentrasi ekstrak lamun (5%, 10% dan 15%). Data dianalisis menggunakan SIDIK RAGAM dan dilakukan analisis lanjut Beda Nyata Jujur (BNJ), apabila terdapat perbedaan pada perlakuan. Ekstrak lamun C. rotundata efektif sebagai antibakteri dengan kategori sedang yaitu zona hambat berkisar antara 5-10 mm. Konsentrasi optimum untuk menghambat S.aureus dan E.coli adalah 15% dengan lama inkubasi 48 jam dengan zona hambat yang dihasilkan masing-masing sebesar 6, 123 mm. Dan 5, 833 mm.Cymodocea rotundata is a type of seagrass that has a potential as an antibacterial. Bioactive compounds which act as such as antibacterial phenols, flavonoids and tannins. The purpose of this study was to determine the effect of different concentration and time of incubation  of seagrass C.rotundata as antibacterial against  S. aureus and E. coli. The method used was experimental laboratories using the basic design of the study completely randomized design (CRD) with factorial pattern (2 factor). The first factor were different concentration (5%, 10% and 15% ) and second factor were time of incubations (24 hours, 48 hours and 72 hours). Data were analyzed using ANOVA and conducted a further test Honestly Significant Difference (HSD), if treatment gave significant effect. C. rotundata extracts had antibacterial activity with medium category which inhibition zone ranges from 5-10 mm. The optimum concentration for inhibiting S. aureus and e. coli were 15% with incubation time of 48 hours resulting inhibition zone 6,123 mm and 5,833 respectively.

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