PENGARUH EKSTRAK JAHE MERAH (Zingiber officinale) DAN MADU TERHADAP KADAR KOLESTEROL TOTAL TIKUS MODEL DIABETES MELITUS

Diabetes mellitus (DM) is a disease characterized by elevated blood glucose levels (hyperglycemia) caused by deficiency of insulin, and insulin resistance or both. This chronic disease prevalence is increasing nationally and globally. This study aimed to determine the effect of ginger extract and honey various doses on levels of total cholesterol in the Wistar diabetic rat model induced by alloxan. This research is true experimental post-test only with control group design. Subject of the study 30 male Wistar rats weight 150-200 grams, divided into 6 groups: A) healthy controls (K1), B) DM control (K2), C) Treatment with red ginger extract 1000 mg / kg and honey 1 ml / kg (K3), D) Treatment with ginger extract red 1000 mg / kg and honey 2 ml / kg (K4), E) Treatment with red ginger extract 500 mg / kg and honey 1 ml / kg (K5), F) Treatment with red ginger extract 500 mg / kg and honey 2 ml / kg (K6). DM induction by alloxan 160 mg / kg intraperitoneally for 5 days, and the provision of treatment for 14 days. Total cholesterol levels were measured by CHOD-PAP method. Results: The mean total cholesterol levels of healthy control group vs the diabetic control 58.20 ± 8.76 vs. 87.80 ± 5.81 mg / dL. Based on one way ANOVA test, red ginger extract and honey various doses significantly lower total cholesterol level (p <0.05). The mean total cholesterol levels between the group K3 to K4 was not statistically different, as well as K5 with K6. However, mean total cholesterol levels at K3 and K4 differ significantly from the K5 and K6. Conclusion: Combination of red ginger extract and honey can lower total cholesterol levels in diabetic rat model induced by alloxan.

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Composite sponges from sheep decellularized small intestinal submucosa for treatment of diabetic wounds

Journal of Biomaterials Applications ◽

10.1177/0885328220963897 ◽

2020 ◽

pp. 088532822096389

Author(s):

Gamze Kara Magden ◽

Cigdem Vural ◽

Busra Yaprak Bayrak ◽

Candan Yilmaz Ozdogan ◽

Halime Kenar

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Rat Model ◽

Small Intestinal Submucosa ◽

Diabetic Rat ◽

Control Group ◽

Significant Difference ◽

Small Intestinal ◽

Intestinal Submucosa ◽

Diabetic Rat Model

Despite the fast development of technology in the world, diabetic foot wounds cause deaths and massive economical losses. Diabetes comes first among the reasons of non traumatic foot amputations. To reduce the healing time of these fast progressing wounds, effective wound dressings are in high demand. In our study, sheep small intestinal submucosa (SIS) based biocompatible sponges were prepared after SIS decellularization and their wound healing potential was investigated on full thickness skin defects in a diabetic rat model. The decellularized SIS membranes had no cytotoxic effects on human fibroblasts and supported capillary formation by HUVECs in a fibroblast-HUVEC co-culture. Glutaraldehyde crosslinked sponges of three different compositions were prepared to test in a diabetic rat model: gelatin (GS), gelatin: hyaluronic acid (GS:HA) and gelatin: hyaluronic acid: SIS (GS:HA:SIS). The GS:HA:SIS sponges underwent a 24.8 ± 5.4% weight loss in a 7-day in vitro erosion test. All sponges had a similar Young’s modulus under compression but GS:HA:SIS had the highest (5.00 ± 0.04 kPa). Statistical analyses of histopathological results of a 12-day in vivo experiment revealed no significant difference among the control, GS, GS:HA, and GS:HA:SIS transplanted groups in terms of granulation tissue thickness, collagen deposition, capillary vessel formation, and foreign body reaction (P > 0.05). On the other hand, in the GS:HA:SIS transplanted group 80% of the animals had a complete epidermal regeneration and this was significantly different than the control group (30%, P < 0.05). Preclinical studies revealed that the ECM of sheep small intestinal submucosa can be used as an effective biomaterial in diabetic wound healing.

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Superoxide dismutase 3 prevents early stage diabetic retinopathy in streptozotocin-induced diabetic rat model

PLoS ONE ◽

10.1371/journal.pone.0262396 ◽

2022 ◽

Vol 17 (1) ◽

pp. e0262396

Author(s):

Ji-Yeon Lee ◽

Mirinae Kim ◽

Su Bin Oh ◽

Hae-Young Kim ◽

Chongtae Kim ◽

...

Keyword(s):

Superoxide Dismutase ◽

Diabetic Retinopathy ◽

Rat Model ◽

Treated Group ◽

Diabetic Rats ◽

Photopic Negative Response ◽

Diabetic Rat ◽

Control Group ◽

Inner Plexiform Layer ◽

Diabetic Rat Model

Purpose To identify the effects of superoxide dismutase (SOD)3 on diabetes mellitus (DM)-induced retinal changes in a diabetic rat model. Methods Diabetic models were established by a single intraperitoneal injection of streptozotocin (STZ) in Sprague-Dawley rats. After purification of the recombinant SOD3, intravitreal injection of SOD3 was performed at the time of STZ injection, and 1 and 2 weeks following STZ injection. Scotopic and photopic electroretinography (ERG) were recorded. Immunofluorescence staining with ɑ-smooth muscle actin (SMA), glial fibrillary acidic protein (GFAP), pigment epithelium-derived factor (PEDF), Flt1, recoverin, parvalbumin, extracellular superoxide dismutase (SOD3), 8-Hydroxy-2’deoxyguanosine (8-OHdG) and tumor necrosis factor-ɑ (TNF-ɑ) were evaluated. Results In the scotopic ERG, the diabetic group showed reduced a- and b-wave amplitudes compared with the control group. In the photopic ERG, b-wave amplitude showed significant (p < 0.0005) reduction at 8 weeks following DM induction. However, the trend of a- and b-wave reduction was not evident in the SOD3 treated group. GFAP, Flt1, 8-OHdG and TNF-ɑ immunoreactivity were increased, and ɑ-SMA, PEDF and SOD3 immunoreactivity were decreased in the diabetic retina. The immunoreactivity of these markers was partially recovered in the SOD3 treated group. Parvalbumin expression was not decreased in the SOD3 treated group. In the diabetic retinas, the immunoreactivity of recoverin was weakly detected in both of the inner nuclear layer and inner plexiform layer compared to the control group but not in the SOD3 treated group. Conclusions SOD3 treatment attenuated the loss of a/b-wave amplitudes in the diabetic rats, which was consistent with the immunohistochemical evaluation. We also suggest that in rod-dominant rodents, the use of blue on green photopic negative response (PhNR) is effective in measuring the inner retinal function in animal models of diabetic retinopathy. SOD3 treatment ameliorated the retinal Müller cell activation in diabetic rats and pericyte dysfunction. These results suggested that SOD3 exerted protective effects on the development of diabetic retinopathy.

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EFEK ANTIDIABETIK EKSTRAK ETANOL DAUN MAHKOTA DEWA (Phaleria macrocarpa) PADA TIKUS DIABETES YANG DIINDUKSI STREPTOZOTOSIN

Biomedika ◽

10.23917/biomedika.v10i2.7019 ◽

2018 ◽

Vol 10 (2) ◽

Cited By ~ 1

Author(s):

Ira Cinta Lestari

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Rat Model ◽

Ethanolic Extract ◽

Diabetic Rat ◽

Control Group ◽

Sprague Dawley ◽

Control Group Design ◽

Phaleria Macrocarpa ◽

Diabetic Rat Model

ABSTRAKDaun mahkota dewa (Phaleria macrocarpa) diketahui memiliki efek anti hiperglikemik dengan menghambat aktivitas enzim pencerna karbohidrat a-glucosidase, namun efeknya pada kondisi diabetes belum diketahui. Penelitian ini bertujuan untuk mengetahui efek antidiabetik ekstrak etanol daun mahkota dewa (EEDMD) terhadap berat badan dan kadar glukosa darah tikus model diabetes. Studi eksperimental dengan rancangan post test only control group design dilakukan terhadap subjek 45 ekor tikus Sprague Dawley. Subjek dikelompokkan dalam kontrol normal, kontrol diabetes diberi pelarut dan diabetes diberi 7mg/200g, 14mg/200g, and 28mg/200g EEDMD secara peroral, sekali sehari selama 3, 14 dan 25 hari. Model tikus diabetes dibuat dengan injeksi streptozotosin dan nicotinamide. Hasil analisa statistik berat badan dan kadar glukosa puasa antar kelompok terdapat perbedaan yang signifikan. Sehingga kesimpulan penelitian ini adalah pemberian EEDMD memiliki efek antidiabetik pada tikus diabetes yang dinduksi stretozotosin.Kata kunci: Diabetes Mellitus, Phaleria Macrocarpa, Ekstrak Etanol, Streptozotosin, Antidiabetik ABSTRACTPhaleria macrocarpa leaf has been known to have anti-hyperglycemic effects by inhibiting the activity of a-glucosidase carbohydrates digestive enzyme, but the systemic effect on diabetic condition is unknown yet. This study was conducted to investigate the antidiabetic effect of ethanolic extract of Phaleria macrocarpa leaf (EEPML) on body weight and blood glucose levels of diabetic rat model. This was a quasi experimental study with post test only control group design. Fourty five male Sprague Dawley rats were classified into normal control group, diabetic control group with solvent, diabetic with 7mg/200g, 14mg/200g, and 28mg/200g of EEPML peroral administration, once a day for 3, 14 and 25 days. The diabetic rat model was made by streptozotocin and nicotinamide injection. Results : Statistical analysis of mean body weight and fasting blood glucose level showed there were significant differences between treatment groups. Conclusion : Administration of EEPML is able to affect the body weight and blood glucose level of diabetic rat model. Keywords: Diabetes Mellitus, Phaleria Macrocarpa, Ethanolic Extract, Streptozotocin, Antidiabetic

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Effects of Wheatgrass (Triticum aestivum) Juice on Serum Cholesterol of Experimentally Induced Hypercholesterolaemic Male Long Evans Rat

Bangladesh Journal of Physiology and Pharmacology ◽

10.3329/bjpp.v27i1-2.20070 ◽

2014 ◽

Vol 27 (1-2) ◽

pp. 21-27

Author(s):

Rokhsana Dil Afroz ◽

Abu Sadat Mohammad Nurunabbi ◽

Md Ismail Khan

Keyword(s):

Triticum Aestivum ◽

Total Cholesterol ◽

Serum Total Cholesterol ◽

Control Group ◽

Medical College ◽

Cholesterol Levels ◽

Healthy Adult Male ◽

The Mean ◽

Long Evans ◽

Experimentally Induced

An experimental study was designed to observe the hypocholesterolaemic effect of wheatgrass (Triticum aestivum) juice on experimentally induced hypercholesterolaemic rats, which was carried out in the Department of Pharmacology & Therapeutics, Dhaka Medical College, Dhaka, from July, 2009 to June, 2010. A total number of 48 healthy adult male Long Evans rats (Norwegian strain), age ranging from 2-3 months, and weighing from 140-200 grams were used for the present study. 6 rats were taken in each group of total 8 groups treated differently with 1 control group. Rats of the first 5 groups were sacrificed at 29th day and their serum total cholesterol levels were measured. Rest of the rats were sacrificed at 57th day and their serum total cholesterol levels were measured. The mean serum concentration of total cholesterol of 10ml/kg grass juice fed normal rats was decreased by 11.64% than that of the laboratory diet fed control group (P<0.01). The total cholesterol of the group of rats treated with 10ml/kg grass juice and1% cholesterol diet were decreased by 36.59% than that of the hypercholesterolaemic control group (P<0.001). The mean total cholesterol of the hypercholesterolaemic rats treated with 10ml/kg grass juice for 28 days were decreased by 29.10%, and in hypercholesterolaemic rats treated with 20ml/kg grass juice for 28 days were decreased by 39.46% (P<0.001). The mean total cholesterol of the hypercholesterolaemic rats treated with 0.14mg/kg atorvastatin for 28 days were found to be decreased by 38.86% than that of hypercholesterolemic control group, which was statistically significant (P<0.001). DOI: http://dx.doi.org/10.3329/bjpp.v27i1-2.20070 Bangladesh J Physiol Pharmacol 2011; 27(1&2):21-27

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Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model

Nutrients ◽

10.3390/nu12082409 ◽

2020 ◽

Vol 12 (8) ◽

pp. 2409 ◽

Cited By ~ 1

Author(s):

Tubagus Bahtiar Rusbana ◽

Afifah Zahra Agista ◽

Wahyu Dwi Saputra ◽

Yusuke Ohsaki ◽

Kouichi Watanabe ◽

...

Keyword(s):

Muscle Atrophy ◽

Rice Bran ◽

Rat Model ◽

Muscle Size ◽

Diabetic Rat ◽

Control Group ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

And Control ◽

Diabetic Rat Model

Fermented rice bran (FRB), a prospective supplement, has been proven to ameliorate certain medical conditions. However, its nutraceutical effect on muscle atrophy has never been investigated. The present study aimed to evaluate the effect of FRB on muscle atrophy in a streptozotocin (STZ)-induced diabetic rat model. Three groups of Sprague-Dawley rats, namely the control, STZ, and FRB groups, were treated as follows. The diabetic groups (STZ and FRB) were injected intraperitoneally with STZ (40 mg/kg BW), whereas the control group was injected with the vehicle. The STZ and control groups were fed the AIN93M diet, and the FRB group was fed 10% of FRB based on the AIN93M diet. The diabetic groups had reduced muscle size compared to the control group; however, these changes were alleviated in the FRB group. Moreover, the FRB group had a significantly lower expression of FBXO32/Atrogin-1 and TRIM63/MuRF1 (p < 0.05) due to blocked NF-κB activation. In conclusion, the anti-inflammatory effect of FRB may be beneficial for ameliorating muscle atrophy in diabetic conditions.

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The Effects of Photobiomodulation on Bone Defect Repairing in a Diabetic Rat Model

International Journal of Molecular Sciences ◽

10.3390/ijms222011026 ◽

2021 ◽

Vol 22 (20) ◽

pp. 11026

Author(s):

Ji-Hua Lee ◽

Su-Chii Kong ◽

Chia-Hsin Chen ◽

Ying-Chun Lin ◽

Kun-Tsung Lee ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Bone Defect ◽

Rat Model ◽

In Vitro Study ◽

Diabetic Rat ◽

Control Group ◽

Calvarial Defect ◽

Diabetic Rat Model

The purpose of this study is to examine the prospective therapeutic effects of photobiomodulation on the healing of bone defects in diabetic mellitus (DM) using rat models to provide basic knowledge of photobiomodulation therapy (PBMT) during bone defect repair. For in vitro study, an Alizzarin red stain assay was used to evaluate the effect of PBMT on osteogenic differentiation. For in vivo study, micro-computed tomography (microCT) scan, H&E and IHC stain analysis were used to investigate the effect of PBMT on the healing of the experimental calvarial defect (3 mm in diameter) of a diabetic rat model. For in vitro study, the high glucose groups showed lower osteogenic differentiation in both irradiated and non-irradiated with PBMT when compared to the control groups. With the PBMT, all groups (control, osmotic control and high glucose) showed higher osteogenic differentiation when compared to the non-irradiated groups. For in vivo study, the hyperglycemic group showed significantly lower bone regeneration when compared to the control group. With the PBMT, the volume of bone regeneration was increasing and back to the similar level of the control group. The treatment of PBMT in 660 nm could improve the bone defect healing on a diabetic rat calvarial defect model.

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Reduced Cholesterol Levels in Adults with Sickle Cell Disease.

Blood ◽

10.1182/blood.v106.11.3808.3808 ◽

2005 ◽

Vol 106 (11) ◽

pp. 3808-3808

Author(s):

Nagaprasad Nagajothi ◽

Jasotha Sanmugarajah

Keyword(s):

Body Mass Index ◽

Sickle Cell Disease ◽

Total Cholesterol ◽

Body Mass ◽

Sickle Cell ◽

Total Cholesterol Level ◽

Control Group ◽

Cell Disease ◽

Cholesterol Levels ◽

The Mean

Abstract Reduced levels of cholesterol was first reported in African children with sickle cell disease (SCD).More recently similar findings were reported in African American adults with SCD in the United States. Epidemiologic studies have identified a relation between low serum levels of total cholesterol (<130 mg/dL) and increased mortality from all causes. We wanted to study the cholesterol levels in adults with SCD especially as their average life expectancy is continuing to increase with advances in medical care.We conducted a retrospective study comparing the serum lipid profile with 15 adults with SCD seen at our hospital with 15 age matched controls who did not have SCD or hyperlipidemia. The mean age in the SCD group was 48±13 years and the mean age in the control group was 46±5years (P=0.73). The mean total cholesterol level in adults with SCD was 135±26 mg/dL and was significantly lower than the mean total cholesterol level in age matched controls which was 169±30 mg/dL (P=0.001). LDL levels were significantly lower in those with SCD compared to controls (67±16 mg/dL versus 90±29mg/dL, respectively, P=0.01). Adults with SCD also had lower HDL levels (45±15 mg/dL) compared with their controls (60±11 mg/dL, P=0.004). There was no difference in the mean triglyceride levels between the two groups. While there was no difference in the mean height between the two groups the SCD group had lower Body Mass Index (BMI) than the control group (23± 4mg/dL versus 27±4mg/dL, respectively, P=0.02). Our results suggest that adults with sickle cell disease have lower serum total cholesterol, LDL, HDL levels as well as lower BMI compared to their age matched controls. Larger studies to validate our observations and to study its clinical implications are warranted. Lipid profile and Body Mass Index in Sickle Cell Disease and Controls Sickle Cell* Control* Mean(SD) Mean(SD) Pvalue *n=15 Age(years) 48±13 46±5 0.73 Total Cholsterol (mg/dL) 135±26 169±30 0.001 LDL(mg/dL) 67±16 90±29 0.01 HDL(mg/dL) 45±15 60±11 0.004 Triglycerides(mg/dL) 113±51 94±47 0.3 Height(cm) 167±7 167±6 0.7 Weight(lbs) 141±27 178±35 0.007 Body Mass Index 23±4 27±4 0.02

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EVALUATION OF HYPOGLYCEMIC ACTIVITY OF PROSOPIS JULIFLORA ON ALLOXAN INDUCED DIABETIC RAT MODEL

INDIAN DRUGS ◽

10.53879/id.56.10.12055 ◽

2019 ◽

Vol 56 (10) ◽

pp. 33-41

Author(s):

M. D Ukande ◽

Krishna Murthy ◽

R. V. Shete ◽

R. S. Solunkhe ◽

Keyword(s):

Rat Model ◽

Leaf Extract ◽

Histopathological Examination ◽

Diabetic Control ◽

Prosopis Juliflora ◽

Hypoglycemic Activity ◽

Diabetic Rat ◽

Control Group ◽

Dependent Manner ◽

Diabetic Rat Model

The present study aimed to evaluate the hypoglycemic activity of Prosopis juliflora leaf extract on Alloxan-induced diabetic rats. Preliminary phytochemical investigation of P. juliflora leaf extract (PJLe) was done according to standard tests. the standardization of PJLe was done by HPTLC analysis. PJLe (100,200,400 mg/kg) was administered to all animals group-wise with standard glibenclamide, Normal saline solution for the normal control group for 21 days orally. After 21 days, one animal from each group were sacrificed. blood samples were collected for biochemical analysis and pancreas tissue was subjected to histopathological examination. Rats treated with PJLe showed significant changes in blood glucose level, body weight, organ weight and biochemical parameters. there was also a significant improvement in the histopathological scores in PJLe treated groups when compared with a diabetic control group. this study evaluates the effectiveness of PJLe as a therapeutic agent in hypoglycemic activity by alloxan-induced diabetic rat model in dose-dependent manner.

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Exploration of the Effect and Mechanism of ShenQi Compound in a Spontaneous Diabetic Rat Model

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666190225113859 ◽

2019 ◽

Vol 19 (5) ◽

pp. 622-631 ◽

Cited By ~ 3

Author(s):

Ya Liu ◽

Jian Kang ◽

Hong Gao ◽

Xiyu Zhang ◽

Jun Chao ◽

...

Keyword(s):

Blood Glucose ◽

Signaling Pathway ◽

Rat Model ◽

Mtor Signaling ◽

Diabetic Rat ◽

Gene Microarray ◽

Mtor Signaling Pathway ◽

Glucose Fluctuation ◽

Blood Glucose Fluctuation ◽

Diabetic Rat Model

Background: Type 2 Diabetes Mellitus (T2DM) is a world-wide metabolic disease with no cure from drugs and treatment. In China, The Traditional Chinese Medicine (TCM) herbal formulations have been used to treat T2DM for centuries. Methods: In this study, we proposed a formula called ShenQi Compound (SQC), which has been used in clinical therapeutics in China for several years. We evaluated the effect of SQC in a spontaneous diabetic rat model (GK rats) by detecting a series of blood indicators and performing histological observations. Meanwhile, the gene microarray and RT-qPCR experiments were used to explore the molecular mechanism of SQC treatment. In addition, western medicine, sitagliptin was employed as a comparison. Results: The results indicated that SQC and sitagliptin could effectively improve the serum lipid (blood Total Cholesterol (TC) and blood Triglycerides (TG)), hormone levels (serum insulin (INS), Glucagon (GC) and Glucagon-Like Peptide-1 (GLP-1)), alleviated the inflammatory response (hypersensitive C-Reactive Protein (hsCRP)), blood glucose fluctuation (Mean Blood Glucose (MBG), standard deviation of blood glucose (SDBG) and Largest Amplitude of plasma Glucose Excursions (LAGE)), pancreatic tissue damage and vascular injury for T2DM. Compared with sitagliptin, SQC achieved a better effect on blood glucose fluctuation (p<0.01). Meanwhile, the gene microarray and RT-qPCR experiments indicated that SQC and sitagliptin may improve the T2DM through affecting the biological functions related to apoptosis and circadian rhythm. Moreover, SQC might be able to influence the mTOR signaling pathway by regulating Pik3r1, Ddit4 expression. Conclusion: All these results indicate that SQC is an effective therapeutic drug on T2DM. Notably, SQC presents an obvious blood glucose fluctuation-preventing ability, which might be derived from the regulation of the mTOR signaling pathway.

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