scholarly journals Formulation of bacterial consortium for improvement growth and yield of maize (Zea mays L.)

2021 ◽  
Vol 18 (1) ◽  
pp. 89
Author(s):  
Ali Ikhwan ◽  
Aniek Iriany ◽  
Erny Ishartati ◽  
Faridlotul Hasanah
Keyword(s):  
Endophytic Bacteria ◽  
Dry Weight ◽  
Bacterial Consortium ◽  
Growth And Yield ◽  
Plant Growth Promoting Bacteria ◽  
Maize Production ◽  
Maize Growth ◽  
Maize Varieties ◽  
Plant Growth Promoting ◽  
Main Plot

Plant growth-promoting bacteria (PGPB) have become an important subject of research to increase maize production. The PGPB consortium should provide more benefits than single or dual inoculation. This study aimed to investigate the effect of a PGPB consortium on improving maize growth and yield. The field experiment used a split-plot design. The main plot consisted of three maize varieties (Talenta, Pertiwi-3, and Bisma), and the subplot consisted of three formulations of PGPB consortia [endophytic bacteria isolates, <em>Acetobacter</em> sp., cellulolytic, and ligninolytic (F1); endophytic bacteria isolates<em>, </em><em>Azospirillum </em>sp., cellulolytic, and ligninolytic (F2); and endophytic bacteria isolates, cellulolytic, ligninolytic, <em>Acetobacter </em>sp., and <em>Azospirillum </em>sp. (F3)] and one control. PGPB consortia formulation did not influence maize growth significantly, but maize varieties did. Pertiwi-3 showed the highest value in all growth variables, followed by Bisma and Talenta, respectively. The effect of PGPB consortia formulation upon ear fresh and dry weight depends upon the maize variety, and Pertiwi-3 showed the highest value in yield variables. PGPB consortia formulation 2 was the most effective to apply for Pertiwi-3 cultivation, while PGPB consortia formulation 3 produced higher yields for Talenta and Bisma. These findings indicated that specific PGPB formula could improve the yield for specific maize varieties.

Utilization of Industrial Waste Pulp And Palm Oil on Growth and Results of Corn (Zea mays L) On Peat

2019 ◽  
Author(s):  
mulono apriyanto bin sugeng rijanto
Keyword(s):  
Fly Ash ◽  
Palm Oil ◽  
Industrial Waste ◽  
Dry Weight ◽  
Growth And Yield ◽  
Randomized Design ◽  
Straight Line ◽  
Maize Varieties ◽  
Main Plot ◽  
Plot Design

This study aims to determine the merits of various industrial waste pulp and palm oil as well as getting the most appropriate formulation on growth and yield of maize varieties Earth-3 and NK-212 in peatlands. Research using split plot design using the design of completely randomized design (CRD) and each treatment was repeated 3 times. Varieties as the main plot consists of: (V1): Varieties of Earth-3, (V2): Varieties of NK-212. Treatment subplot, consisting of six formulations ameliorant, namely: F1 (60% OPEF + 20% GPB + 10% Dregs + 10% Fly ash), F2(60% OPEF + 10% GPB + 20% Dregs + 10% Fly ash ), F3(60% OPEF + 10% GPB + 10% Dregs + 20% Fly ash), F4(40% OPEF + 30% GPB + 10% Dregs + 20% Fly ash), F5(40% OPEF + 20% GPB + 30% Dregs + 10% Fly ash) and F6(40% OPEF + 10% GPB + 20% Dregs + 30% Fly ash). The data obtained were statistically analyzed using analysis of variance (ANOVA) followed by HSD test at 5% level.The results showed that various formulations of industrial waste pulp and palm oil gave similar results to the root dry weight, dry weight of straw, hay root ratio, the diameter of the cob, corncob berkelobot, corncob without the husk and dry seed weight. It can be seen from the cob generated a large and long and close cob husk well (± 98%), beans straight line with a number of seed rows 15-16 rows, as well as a uniform crop diversity, is in conformity with the description.

scholarly journals Response of Zea mays var. Jala to Burkholderia sp. and Klebsiella oxytogena under reduced urea dose

2020 ◽  
Vol 12 (2) ◽  
pp. 173-182
Author(s):  
Dieter Plata-Guzmán ◽  
Juan Luis Ignacio-de la Cruz ◽  
Juan Carlos Carrillos-Amezcua ◽  
Juan Manuel Sánchez-Yáñez
Keyword(s):  
Zea Mays ◽  
Nitrogen Fertilizer ◽  
Block Design ◽  
Dry Weight ◽  
Soil Conditions ◽  
Plant Growth Promoting Bacteria ◽  
Maize Production ◽  
Maize Seeds ◽  
Randomized Block Design ◽  
Maize Varieties

Introduction: The growing of maize (zea mays L.) in Latin America is carried out under the intensive production system, which causes loss of soil fertility due to excessive nitrogen fertilizer. Objective: Analyzing the response of maize var. Jala to genera of plant growth promoting bacteria (PGPB) endophytes of maize var. Mexicana (teocintle) when using nitrogen fertilizer such as urea at 50 % under greenhouse and soil conditions. Methodology: A randomized block design was established with five treatments, three controls and twenty repetitions. The treatments with inoculated maize seeds and 50 % urea were: Burkholderia (KO) and Burkholderia sp. 14 (B14); the controls were: non-inoculated maize with 50 % urea (RC1), non-inoculated maize irrigated with water (FC) and non-inoculated maize with 100 % urea (RC2). The experiments were established under greenhouse and soil conditions. The response variables were radical dry weight (RDW) and fresh weight of grain (FWG). ANOVA and Tukey’s mean comparison (P ≤ 0.01) were carried out. Results: In both environments, treatment B14 showed statistically higher values than those obtained with FC (full control), but statistically equal to RC2 (relative control). Study limitations: Maize varieties have specific and beneficial endophytic PGPB genera, which are not necessarily for regional varieties. Originality: PGPB endophytes from teocintle (ancestor of maize) were used because of their close genetic relationship with the maize var. Jala. Conclusion: PGPB endophytes from teocintle are a viable option for sustainable maize production.

Assessing the Plant Growth-promoting Traits and Host Specificity of Endophytic Bacteria of Pulse Crops

2021 ◽  
Author(s):  
R. Thamizh Vendan ◽  
D. Balachandar
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing ◽  
Plant Growth Promoting Traits

Background: Symbiotic associations between legumes and Rhizobia are ancient and fundamental. However, the plant growth-promoting endophytes other than Rhizobia are not yet fully explored for pulses productivity. The present study was aimed to isolate efficient endophytic bacteria from pulses, assess their diversity, screen their plant growth-promoting activities and to test their potential as bio inoculants for pulses.Methods: We have isolated several endophytic bacteria from pulse crops more specifically from blackgram (Vigna mungo) and greengram (Vigna radiata). After careful screening, 15 promising endophytic isolates were selected for this study. The identification of endophytic bacterial isolates was performed by 16S rRNA gene sequencing. The isolates were tested for their potential for the plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore secretion and antifungal activity. Pot culture experiments were conducted with the screened potential endophytic cultures.Result: The 16S rRNA gene sequencing revealed that species of Enterobacter, Bacillus, Pantoea, Pseudomonas, Acromobacter, Ocrobacterium were found as endophytes in blackgram and greengram. The in vitro screening identified Bacillus pumilus (BG-E6), Pseudomonas fluorescens (BG-E5) and Bacillus licheniformis (BG-E3) from blackgram and Pseudomonas chlororaphis (GG-E2) and Bacillus thuringiensis (GG-E7) from greengram as potential plant growth-promoting endophytes. These strains showed antagonism against plant pathogenic fungi. Upon inoculation of these endophytic PGPR strains, the blackgram and greengram growth and yield got increased. Among the strains, BG-E6 recorded 14.7% increased yield in blackgram and GG-E2 accounted for a 19.5% yield increase in greengram compared to respective uninoculated control. The experimental results showed that there was a host specificity found among the endophytic bacterial cultures with pulses. The cross inoculation of endophytic strains did not perform well to enhance the growth and yield of their alternate hosts. 

Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L).

2018 ◽  
Vol 20 (1) ◽  
pp. 9
Author(s):  
Ryan Hilda Wandita ◽  
Sri Pujiyanto ◽  
Agung Suprihadi ◽  
Ratih Dewi Hastuti
Keyword(s):  
Plant Growth ◽  
Traditional Medicine ◽  
Allium Cepa ◽  
Endophytic Bacteria ◽  
Economic Value ◽  
Average Diameter ◽  
Plant Growth Promoting Bacteria ◽  
Allium Cepa L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN

scholarly journals Pertumbuhan dan Produksi Dioscorea sansibarensis Pax pada Perlakuan Panjang lanjaran dan Waktu Batang Membelit*

2018 ◽  
Vol 45 (3) ◽  
pp. 249
Author(s):  
Solikin ,
Keyword(s):  
Block Design ◽  
Soil Surface ◽  
Dry Weight ◽  
Growth And Yield ◽  
Botanic Garden ◽  
Randomized Block Design ◽  
Two Factors ◽  
Main Plot ◽  
Total Plant ◽  
Total Dry Weight

Dioscorea is potentially used as staple food to support food security. The research was aimed to determine the effect of stake length and time of  stem twining  on  the growth of Dioscorea sansibarensis Pax. The experiment was conducted  in  Purwodadi Botanic Garden from December 2014 until May 2015 using split plot randomized block design consisted of two factors, i.e. the stake length and time of stem twining. The stake length was the main plot consisted of 150 cm, 100 cm and 50 cm above soil surface and without stake (control). The time of stem twining as subplots, i.e. stem twined early, twined at the time of 4 leaves stage, and twined at 8 leaves stage. Each combination of the treatments was replicated three times. The results showed that there was significant effect on the stake length  and the time of stem twining treatments on the plant growth and yield. The stake  length of 150 cm treatment produced the highest fresh tuber and total plant dry weight, i.e., 257.24 g and 132.77 g per plant, respectively. On the contrary, the plant without stake produced the lowest fresh tuber and  total dry weight of plant, i.e., 112.10 g and 48.65 g per plant, respectively.<br /><br />Keywords: biomass,leaf area, photosyntesis, tuber weight

scholarly journals Growth and Yield Response of Rodent Tuber (Typhonium flagelliforme (Lodd.) Blume) under Different Light Intensities and Concentrations of Paclobutrazol

2016 ◽  
Vol 1 (3) ◽  
pp. 29-35
Author(s):  
Noorwitri Utami ◽  
Lukita Devy ◽  
Arief Arianto
Keyword(s):  
Light Intensity ◽  
Concentration Level ◽  
Dry Weight ◽  
Leaf Length ◽  
Growth And Yield ◽  
Yield Response ◽  
Full Sunlight ◽  
Anticancer Treatment ◽  
Main Plot ◽  
Number Of Leaves

Rodent tuber (Typhonium flagelliforme (Lodd) Blume) is one of the medicinal plants used for anticancer treatment, but the information on the cultivation of the plant is limited. The objectives of this research was to study the effect of light intensity and concentration level of paclobutrazol on growth and yield of rodent tuber. This research was conducted at Puspiptek Serpong, Tangerang Selatan, Indonesia. This experiment arranged in a split plot design with three replications. The main plot is light intensity (35, 55, and 100%). The sub plot is concentration level of paclobutrazol (0, 50, 100, and 150 ppm). Paclobutrazol applied as soil drench at one month after planting. In each application 500 ml solution was used. Variables observed consisted of plant height, number of leaves, leaf length, leaf width, leaf and tuber fresh weight; and leaf and tuber dry weight. Data were analyzed using analysis of variance and Duncan Multiple Range test at 5%. The result showed that interaction between light intensity and paclobutrazol affected the whole observed variable. Dry weight of tuber under 35% light intensity was lower than those in other light intensity for all treatments of paclobutrazol. Full sunlight intensity showed better dry weight in all treatments of paclobutrazol. However the dry weight decreasing significantly at 150 ppm paclobutrazol, but still higher than those in 35% light intensity. Therefore, rodent tuber is better cultivated under full sunlight and 55% light intensity with 100 ppm paclobutrazol.

scholarly journals Effect of Pyroligneous Acid on the Microbial Community Composition and Plant Growth-Promoting Bacteria (PGPB) in Soils

Soil Systems ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 10
Author(s):  
Anithadevi Kenday Sivaram ◽  
Logeshwaran Panneerselvan ◽  
Kannappar Mukunthan ◽  
Mallavarapu Megharaj
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Microbial Diversity ◽  
Growth And Yield ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Microbial Composition ◽  
Pyroligneous Acid ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pyroligneous acid (PA) is often used in agriculture as a plant growth and yield enhancer. However, the influence of PA application on soil microorganisms is not often studied. Therefore, in this study, we investigated the effect of PA (0.01–5% w/w in soil) on the microbial diversity in two different soils. At the end of eight weeks of incubation, soil microbial community dynamics were determined by Illumina-MiSeq sequencing of 16S rRNA gene amplicons. The microbial composition differed between the lower (0.01% and 0.1%) and the higher (1% and 5%) concentration in both PA spiked soils. The lower concentration of PA resulted in higher microbial diversity and dehydrogenase activity (DHA) compared to the un-spiked control and the soil spiked with high PA concentrations. Interestingly, PA-induced plant growth-promoting bacterial (PGPB) genera include Bradyrhizobium, Azospirillum, Pseudomonas, Mesorhizobium, Rhizobium, Herbaspiriluum, Acetobacter, Beijerinckia, and Nitrosomonas at lower concentrations. Additionally, the PICRUSt functional analysis revealed the predominance of metabolism as the functional module’s primary component in both soils spiked with 0.01% and 0.1% PA. Overall, the results elucidated that PA application in soil at lower concentrations promoted soil DHA and microbial enrichment, particularly the PGPB genera, and thus have great implications for improving soil health.

scholarly journals Burkholderia Phytofirmans PsJN Stimulate Growth and Yield of Quinoa under Salinity Stress

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 672 ◽  
Author(s):  
Aizheng Yang ◽  
Saqib Saleem Akhtar ◽  
Qiang Fu ◽  
Muhammad Naveed ◽  
Shahid Iqbal ◽  
...  
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Endophytic Bacteria ◽  
Ion Homeostasis ◽  
Growth And Yield ◽  
Shoot Biomass ◽  
World Population ◽  
Plant Growth Promoting Bacteria ◽  
Ros Scavenging ◽  
Burkholderia Phytofirmans Psjn

One of the major challenges in agriculture is to ensure sufficient and healthy food availability for the increasing world population in near future. This requires maintaining sustainable cultivation of crop plants under varying environmental stresses. Among these stresses, salinity is the second most abundant threat worldwide after drought. One of the promising strategies to mitigate salinity stress is to cultivate halotolerant crops such as quinoa. Under high salinity, performance can be improved by plant growth promoting bacteria (PGPB). Among PGPB, endophytic bacteria are considered better in stimulating plant growth compared to rhizosphere bacteria because of their ability to colonize both in plant rhizosphere and plant interior. Therefore, in the current study, a pot experiment was conducted in a controlled greenhouse to investigate the effects of endophytic bacteria i.e., Burkholderia phytofirmans PsJN on improving growth, physiology and yield of quinoa under salinity stress. At six leaves stage, plants were irrigated with saline water having either 0 (control) or 400 mM NaCl. The results indicated that plants inoculated with PsJN mitigated the negative effects of salinity on quinoa resulting in increased shoot biomass, grain weight and grain yield by 12%, 18% and 41% respectively, over un-inoculated control. Moreover, inoculation with PsJN improved osmotic adjustment and ion homeostasis ability. In addition, leaves were also characterized for five key reactive oxygen species (ROS) scavenging enzyme in response to PsJN treatment. This showed higher activity of catalase (CAT) and dehydroascobate reductase (DHAR) in PsJN-treated plants. These findings suggest that inoculation of quinoa seeds with Burkholderia phytofirmans PsJN could be used for stimulating growth and yield of quinoa in highly salt-affected soils.

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