scholarly journals PRODUKSI ENZIM SELULASE OLEH AKTINOMISET MENGGUNAKAN FROND SAGU

2019 ◽  
Vol 16 (2) ◽  
pp. 80
Author(s):  
Sumarni Nompo ◽  
Anja Meryandini ◽  
Titi Candra Sunarti
Keyword(s):  
Solid State ◽  
Room Temperature ◽  
Specific Activity ◽  
Cellulase Production ◽  
Raw Material ◽  
Submerged Cultivation ◽  
Cellulose Content ◽  
Cellulase Enzymes ◽  
Specific Activities ◽  
High Cellulose

<p>Fround Sagu adalah pucuk batang sagu yang masih dibungkus oleh pelepah dan tidak diamnfaatkan oleh industri pengolahan sagu. Fround sagu memiliki kandungan serat dengan kandungan selulosa yang tinggi serta berpotensi dijadikan bahan baku untuk produksi selulase. Enzim selulase diproduksi melalui kultivasi substrat cair frond sagu oleh Aktinomiset. Subtart berupa tepung sagu dan ampas frond sagu, diinokulasi oleh isolat HJ4 (3b) dan HJ5 (4b). Kedua isolat diremajakan dalam medium ISP-4 selama 5 hari, kemudian diinokulasikan ke dalam media tepung frond sagu dan ampas frond dan diinkubasi dalam shaker pada suhu runag selama 9 hari. Kedua isolat Aktinomiset mampu menghasilkan enzim selulase pada kedua substrat dan metode kultivasi. Isolat HJ4 (3b) dan HJ4 (5b) pada perlakuan kultivasi substrat padat ampas frond sagu menghasilkan aktivitas spesifik yaitu endoglukase (CMCase) tertinggi yaitu 0.314 U mg-1 dan 0.294 U mg-1 dan aktivitas spesifik enzim eksoglukanase (FPase) yaitu 0.269 U mg-1 dan 0.258 U mg-1, sedangkan pada perlakuan kultivasi substat padat menggunakan tepung frond sagu dihasilkan aktivitas spesifik endoglukanase masing-masing sebesar 0.258 U mg-1 dan 0.254 U mg-1 serta aktivitas spesifik eksoglukanase 0.205 U mg-1 dan 0.198 U mg-1.</p><p> </p><p><strong>Production of Cellulase Enzyme by Actinomycet Using Sago Frond</strong></p><p>Sago frond is the upper part of sago trunk which is still wrapped by leaflet, and is not used by the sago processing industry. Sago frond contains fiber with high cellulose content that could potentially be used by as raw material for cellulase production. Cellulase enzymes were produced through both solid-state and submerged cultivation of sago frond by Actinomicycetes. Two substrates, sago frond flour and pulp of sago fronds, were inoculated by isolate HJ4 (3b) and HJ4 (5b). Both isolates were rejuvenated in Sp-4 medium for 5 days, then were inoculated into substrate of frond flour and hampas, and were incubated in a shaker at room temperature for 9 days. Both Actinomycetes isolates were able to produce cellulase enzymes by using both substrates and cultivation methods. The isolates of HJ4 (3b) and HJ4 (5b) by using pulp and solid-state cultivation produced the highest endoglucanase (CMCase) specific activity of 0.294 U mg-1 and 0.276 U mg-1 and exoglucanase (FPase) substrate specific activity os 0.252 U mg-1 and 0.241 U mg-1, while in the solid-state cultivation and by using sago fronds flour resulted in specific endoglucanase activities which were 0.242 U mg-1 and 0.238 U mg-1 and exoglucanase specific activities 0.192 U mg-1 and 0.185 U mg-1, respectively.</p>

scholarly journals Effect of various pretreatments of oil palm empty fruit bunch fibres for subsequent use as substrate on the performance of cellulase production by Aspergillus terreus

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 291-307
Author(s):  
Mahdi Shahriarinour ◽  
Mohd Noor Abdul Wahab ◽  
Shuhaimi Mustafa ◽  
Rosfarizan Mohamad ◽  
Arbakariya B. Ariff
Keyword(s):  
Phosphoric Acid ◽  
Oil Palm ◽  
Aspergillus Terreus ◽  
Cellulase Production ◽  
Cellulose Content ◽  
Empty Fruit Bunch ◽  
Effective Microorganisms ◽  
Chemical Pretreatments ◽  
Main Components ◽  
High Cellulose

The possibility of using treated oil palm empty fruit bunch (OPEFB) fibres as substrate for cellulase production by Aspergillus terreus was studied using shaking flask fermentation. The effect of different chemical pretreatments, i.e. formic acid, acetic acid, propylamine, phosphoric acid, and n-butylamine, on the suitability of OPEFB fibres as fermentation substrate was investigated. The findings revealed that pretreatment with these chemicals significantly (P<0.05) increased the cellulose and reduced the lignin contents prior to enzymatic hydrolysis. However, fermentation using OPEFB fibres pretreated with phosphoric acid gave the highest cellulase production, which was related to high cellulose content. Further improvement in cellulase production was obtained when the chemically pretreated OPEFB fibres were subsequently treated hydrothermally (autoclaved at 160oC for 10 min) and then biologically (using effective microorganisms). The final activity of the three main components of cellulase (FPase, CMCase, and β-glucosidase) obtained in fermentation by A. terreus using optimally treated OPEFB fibres was (0.77 U mL−1, 8.5 U mL-1, and 6.1 U mL-1), respectively. The production of all these three major components of cellulase using pretreated OPEFB fibres (i.e. chemical, hydrothermal, and biological) were about three times higher than those obtained from fermentation using untreated OPEFB fibres.

Cellulase production by white-rot basidiomycetous fungi: solid-state versus submerged cultivation

2018 ◽  
Vol 102 (14) ◽  
pp. 5827-5839 ◽  
Author(s):  
Joseph A. Bentil ◽  
Anders Thygesen ◽  
Moses Mensah ◽  
Lene Lange ◽  
Anne S. Meyer
Keyword(s):  
Solid State ◽  
Cellulase Production ◽  
White Rot ◽  
Submerged Cultivation

scholarly journals THE EFFECT OF THE TYPE OF ACID CATALYST AND TIME ON % YIELD OF BIOETHANOL FROM ELEPHANT GRASS (Pennistum Purpureum Schumach)

2020 ◽  
Vol 4 (2) ◽  
pp. 19
Author(s):  
Netty - Herawati
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acid Catalyst ◽  
Raw Material ◽  
Cellulose Content ◽  
Bioethanol Production ◽  
Elephant Grass ◽  
Fermentation Time ◽  
Cattle Feed ◽  
Good Nutrition ◽  
High Cellulose

Elephant gass is cattle feed that contains good nutrition. One of its uses is converted into an energy source in the form bioethanol, Elephant grass has a high cellulose content reaching 40,85%, therefore elephant grass has the potential to be used as raw material in manufacture of bioethanol through the process of acid hydrolysis and fermentation. In research on percent yield of bioethanol from elephant grass chemically carried out at fixed conditions : grass weight 100 gr, temperature 100oC, water 1 liter, H2SO4 30 ml, hydrolysis timw 2 hours and conditions change : fermentation time 4,6,8 (day), saccharomyces cerevisiae starter 7%, 9%, 11%, 13%, HCl and H2SO4 catalys. From the research on chemical bioethanol production from elephant grass we got the best percent yield at 6 days of fermentation, 11% saccharomyces cerevisiae, HCl catalys which was 17,30%Keywords: bioethanol, fermentation, elephant grass,

scholarly journals Utilization Of Tobacco Stem (Nicotiana tabaccum L) As Tray Egg Filler

2020 ◽  
Vol 1 (2) ◽  
pp. 1-7
Author(s):  
Andrew Setiawan Rusdianto ◽  
Winda Amilia ◽  
Fatma Dewi
Keyword(s):  
Raw Material ◽  
Pulp Fiber ◽  
Cellulose Content ◽  
Significance Level ◽  
Tobacco Stem ◽  
Naoh Treatment ◽  
Chicken Eggs ◽  
High Cellulose ◽  
Range Test

Generally only used the leaves of tobacco plants as raw material for cigarette and stems discarded as waste. The use of waste tobacco rod has the potential to be converted into primary packaging such as trays range chicken eggs because it has a high cellulose content. Tobacco rod contains a relatively high amount of cellulose, reaching 56.10 percent of dried tobacco stalks. The process is done in making pulp fiber or tobacco stem malalui process of alkali (NaOH) with specific concentration to produce pulp that is good. The use of NaOH aims to degrade lignin by means of partial overhaul of bond. The existence of lignin in the pulp generally unfavorable effect on the quality of pulp for fiber becomes rigid, Statistical data analysis using ANOVA If treatment showed differences do a further test using Duncan's Multiple Range Test (DMRT) at significance level of 5 percent. The data generated is the highest grammage at 5 percent NaOH treatment amounted to 201.1 g.m-2 and the lowest 15 percent NaOH treatment 132.8 g.m-2, the highest thickness of 5 percent NaOH treatment of 2.509 mm and the lowest 15percent NaOH treatment of 1,835 mm. The highest density of 5 percent NaOH treatment of 80.81 kg.m-3 and the lowest at 10 percent NaOH treatment of 57.61 kg.m-3. While the highest tensile resistance in the treatment of 10percent NaOH 76.27 N.m-1 and the lowest at 5 percent NaOH treatment is 45.82 N.m-1, the highest thickness of 5percent NaOH treatment of 2.509 mm and the lowest 15 percent NaOH treatment of 1,835 mm. The highest density of 5 percent NaOH treatment of 80.81 kg.m-3 and the lowest at 10 percent NaOH treatment of 57.61 kg.m-3. While the highest tensile resistance in the treatment of 10 percent NaOH 76.27 N.m-1 and the lowest at 5 percent NaOH treatment is 45.82 N.m-1, the highest thickness of 5 percent NaOH treatment of 2.509 mm and the lowest 15 percent NaOH treatment of 1,835 mm. The highest density of 5 percent NaOH treatment of 80.81 kg.m-3 and the lowest at 10 percent NaOH treatment of 57.61 kg.m-3. While the highest tensile resistance in the treatment of 10 percent NaOH 76.27 N/m and the lowest at 5 percent NaOH treatment is 45.82 N.m-1

scholarly journals Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaete chrysosporium

2017 ◽  
Vol 1 (1) ◽  
pp. 17
Author(s):  
Sri Rulianah ◽  
Zakijah Irfin ◽  
Mufid Mufid ◽  
Prayitno Prayitno
Keyword(s):  
Enzyme Activity ◽  
Salicylic Acid ◽  
Phanerochaete Chrysosporium ◽  
Substrate Concentration ◽  
Lignin Content ◽  
Raw Material ◽  
Cellulase Enzymes ◽  
Fermentation Time ◽  
Acid Reagent ◽  
High Cellulose

Bagasse mengandung selulosa yang cukup tinggi sehingga berpotensi sebagai bahan baku produksi crude selulase menggunakan kapang Phanerochaete chrysosporium. Kapang ini memiliki kemampuan untuk memproduksi enzim selulase dari substrat yang mengandung selulosa dan juga menghasilkan enzim yang dapat memecah lignin sehingga tidak perlu dilakukan proses delignifikasi. Tujuan dari penelitian ini adalah untuk memanfaatkan limbah ampas tebu sebagai bahan baku pembuatan crude selulase menggunakan kapang Phanerochaete chrysosporium dan mengetahui pengaruh penambahan konsentrasi substrat dan waktu fermentasi terhadap aktivitas crude selulase yang dihasilkan. Penelitian ini dilakukan dengan cara mengeringkan dan memperkecil ukuran ampas tebu, meremajakan kapang Phanerocheate chrysoporium, membuat inokulum dalam media cair, memfermentasi ampas tebu sesuai dengan variabel, dengan media Nitrogen Limited Media (NLM) menggunakan kapang Phanerocheate chrysoporium. Hasil fermentasi disaring, dan filtratnya dianalisa aktivitasnya sebagai crude selulase. Variabel dalam penelitian ini adalah waktu fermentasi 9, 11, 13, 15 dan 17 hari dan konsentrasi ampas tebu sebagai media: 5, 6, dan 7 % b/v. Ekstrak kasar selulase (crude) yang dihasilkan disaring menggunakan filter vakum, dan aktivitas filtrat (crude cellulase) diuji dengan pereaksi DNS (dinitro salicylic acid) dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa aktivitas selulase tertinggi diperoleh pada variabel konsentrasi ampas tebu sebesar 7% b/v dan waktu inkubasi selama 17 hari yaitu sebesar 91.304 U/mL.Bagasse contain high cellulose which potentially to be used to raw material for producing cellulase enzyme using fungi Phanerochaete chrysosporium. This fungus has ability to produce cellulase enzymes from substrates which contain cellulose and also produce enzymes that can degrade lignin content so it didn’t need the delignification process. The objective of this study was to convert cellulose in bagasse to be crude cellulase enzymes by using Phanerochaete chrysosporium and determine the effect of substrate concentration and fermentation time to the enzyme activity. This research was conducted by drying and reducing the bagasse particle size, rejuvenating mold Phanerocheate chrysoporium, making inoculum in liquid medium, fermenting bagasse in accordance with the variable, with media NLM (nitrogen limited media) using Phanerocheate chrysoporium. Fermentation results were filtered, and it was analyzed the activity of crude cellulase. The variable in this study was the time of fermentation 9, 11, 13, 15, and 17 days and substrate concentration: 5, 6, and 7 % b/v. Crude cellulose was filtered and was analyzed the enzyme activity by DNS (dinitro salicylic acid) reagent, using UV-Vis spectrophotometer. The best result of this study was the crude cellulase with highest activity 91,304 U/mL for 7 % substrate concentration with fermentation time 17 days.

scholarly journals Pemanfaatan Jerami Padi (Oryza Sativa L.) Sebagai Bahan Baku Dalam Pembuatan CMC (Carboximetil Cellulose)

2021 ◽  
Vol 15 (2) ◽  
pp. 194
Author(s):  
RIZKA NURLAILA
Keyword(s):  
Oryza Sativa ◽  
Reaction Time ◽  
Rice Straw ◽  
Carboxymethyl Cellulose ◽  
Oryza Sativa L ◽  
Raw Material ◽  
Cellulose Content ◽  
Cosmetic Products ◽  
High Cellulose

Rice straw is a waste from rice plants that contains 37.71% cellulose, 21.99% hemicellulose, and 16.62% lignin. High cellulose content in rice straw can be used as raw material for the manufacture of Carboxymethyl Cellulose (CMC). CMC is a cellulose derivative widely used in food, pharmaceutical, detergent, textile and cosmetic products industries as a thickener, stabilizer of emulsions, or suspensions and bonding. This study aims to process rice straw waste into CMC with variations in sodium monochloroacetate of 5,6,7,8 and 9 grams. The method used in this research is by synthesis using 15% NaOH solvent, with a reaction time of 3.5 hours and 5 grams of rice straw. The results showed that the best CMC was obtained at a concentration of 9 grams of sodium monochloroacete with a yield characterization of 94%, pH 6, water content of 13.39%, degree of substitution (Ds) of 0.80, and viscosity of 1.265 cP.

scholarly journals Production of antipollutant mask with bamboo based carbon activated using H3PO4 and K2CO3

2018 ◽  
Vol 67 ◽  
pp. 03041
Author(s):  
Mahmud Sudibandriyo ◽  
Shobrun Jamil
Keyword(s):  
Activated Carbon ◽  
Adsorption Capacity ◽  
Dip Coating ◽  
Raw Material ◽  
Bet Surface Area ◽  
Cellulose Content ◽  
Pollution Problem ◽  
Gas Mask ◽  
Safety Limit ◽  
High Cellulose

The increase of developing countries’ economic level has led to increase in air pollution problem. This research aims to make activated carbon based gas mask filter that was prepared from bamboo scraps by the combined activation using H3PO4 and K2CO3.Bamboo was selected as raw material because of its abundant availability and high cellulose content (42.4-53.6%).Dip coating was conducted to coat activated carbon on the surface layer of mask by adding TEOS compound.Furthermore, adsorption capacity of activated carbon was tested using compartment by flowing air containing CO and CO2 for one hour. The results of the characterization shows that the iodine number of the activated carbon produced reaches 916.3 mg/g with BET surface area of 465.2 m2/g. SEM-EDX analysis shows that the carbon content is 74.83%wt.Adsorption capacity of activated carbon was tested using compartment by flowing air containing pollutant gas and compressed air for one hour. The results indicate that the maximum number of moles CO2 adsorbed is 4.8 mmol/g with 7 hour saturated time,while adsorption capacity of CO measured in 1 hour test is 0.103 mmol/g. Therefore, activated carbon has met the standards and can be applied for gas mask filter to eliminate CO and CO2 until below safety limit concentration.

scholarly journals FERMENTASI GLUKOSA HASIL HIDROLISIS BUAH KUMBI UNTUK BAHAN BAKU BIOETANOL

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Sri Seno Handayani ◽  
Surya Hadi ◽  
Haryanti Patmala
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fuel Mixture ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Cellulose Content ◽  
The Family ◽  
C 32 ◽  
C 30 ◽  
Bioethanol Yield ◽  
High Cellulose

Abstrak. Kumbi atau Voacanga foetida (blume) rolfe merupakan tumbuhan dari family Apocynaceae. Pulau Lombok merupakan daerah utama tempat tumbuhnya ‘Kumbi’ ini. Kumbi memiliki kandungan selulosa yang cukup tinggi. Selulosa merupakan salah satu bahan baku dalam pembuatan bioetanol. Sebelumnya telah dilakukan penelitian mengenai pembuatan bioetanol dari buah Kumbi (Voacanga foetida (blume) rolfe) ini dengan kadar 14% dan % rendemen sebesar 14,793%. Hasil yang didapatkan ini sudah cukup tinggi namun belum memenuhi syarat untuk dijadikan sebagai bahan baku campuran pada bahan bakar. Tujuan dari penelitian ini untuk mengetahui pengaruh suhu fermentasi terhadap rendemen bioetanol dari buah kumbi menggunakan Saccharomyces cerevisiae dan Rhyzopus oryzae. Metode yang digunakan adalah hidrolisis dengan HCl dan fermentasi menggunakan Saccharomyces cerevisiae dan Rhyzopus oryzae. Berdasarkan penelitian yang telah dilakukan, bioetanol dari buah kumbi dapat diproduksi melalui proses hidrolisis dan fermentasi menggunakan Saccharomyces cerevisiae dan Rhyzopus oryzae dengan variasi suhu fermentasi 28°C, 30°C, 32°C, 34°C, 36°C, 38°C dan 40°C, dan destilasi pada suhu 78 oC, menghasilkan rendemen bioetanol kasar pada suhu optimum 36 °C sebesar 66,02% (w/w) menggunakan Saccharomyces cerevisiae sedangkan dengan rasio yang sama menggunakan Rhyzopus oryzae suhu optimum dicapai pada 32°C, menghasilkan rendemen bioetanol kasar sebesar 88,14% (w/w). Kata kunci: Bioetanol, Buah Kumbi, Hidrolisis, Fermentasi      Abstract.    Kumbi or Voacanga foetida (blume) Rolfe is a plant of the family Apocynaceae.  Lombok Island is the main area where the growth of Kumbi. Kumbi has a high cellulose content. Cellulose is a raw material in the manufacture of bioethanol. Research for bioethanol production from fruit Kumbi (Voacanga foetida (blume) Rolfe) generates yield of 14.793%. These results are not yet eligible to be used as raw material in the fuel mixture. This study aims to determine the effect of temperature on the yield of bioethanol fermentation of fruit Kumbi using Saccharomyces cerevisiae and Rhyzopus oryzae. The method used is by HCl hydrolysis and fermentation with Saccharomyces cerevisiae and Rhyzopus oryzae. The results showed that ethanol from Kumbi fruit can be produced by the hydrolysis and fermentation using Saccharomyces cerevisiae and Rhyzopus oryzae. Fermentation temperature variation used was 28 °C, 30 °C, 32 °C, 34 °C, 36 °C, 38 °C, 40 °C and distilled at a temperature of 78 C. The yield of bioethanol 66.02% (w/w ) obtained using Saccharomyces cerevisiae at a temperature of 36 °C while the same ratio used Rhyzopus oryzae produce bioethanol yield of 88.14% (w / w) at a temperature of 32 oC.Keywords: Bioethanol, Fruit Kumbi, hydrolysis, fermentation

An International Collaborative Study to Investigate Standardisation of Hirudin Potency

1993 ◽  
Vol 69 (05) ◽  
pp. 430-435 ◽  
Author(s):  
Colin Longstaff ◽  
Man-Yu Wong ◽  
Patrick J Gaffney
Keyword(s):  
Specific Activity ◽  
Collaborative Study ◽  
Residual Activity ◽  
Quality Standard ◽  
Upper Limit ◽  
Assay Procedure ◽  
Specific Activities ◽  
International Collaborative ◽  
Range Of Values ◽  
International Collaborative Study

SummaryAn international collaborative study has been carried out to investigate the reproducibility of hirudin assays in 13 laboratories using four recombinant hirudins and one natural, sulphated product. A simple assay procedure was proposed involving the titration of α-thrombin with inhibitor and measurement of residual activity using a chromogenic substrate. A standard α-thrombin preparation was supplied to ensure that this reagent was of uniform quality throughout the study. The method appeared to present no difficulties and laboratories reported similar potencies for the 5 hirudin samples, in line with expected values. This gave 200–222 Thrombin Inhibitory Units/ampoule (TIU/ampoule) of lyophilised hirudin, with geometric coefficient of variation (gcv) values ranging from 10.15–15.97%. This corresponds to specific activities of approximately 14,300–15,900 TIU/mg protein. This is close to the upper limit of previously reported values of specific activity. We conclude that the precision of this determination compared with the wider range of values in the literature (8,000–16,000 thrombin inhibitory units [TIU]/mg) results from the use of good quality standard α-thrombin by all laboratories. This study has important implications for hirudin standardisation.

Improved Sol-Gel Materials for Efficient Solid State Dye Lasers

1993 ◽  
Vol 329 ◽  
Author(s):  
Michael Canva ◽  
Patrick Georges ◽  
Jean-Fran^ois Perelgritz ◽  
Alain Brun ◽  
Fréddric Chaput ◽  
...  
Keyword(s):  
Solid State ◽  
Physical Properties ◽  
Room Temperature ◽  
Sol Gel ◽  
Tunable Lasers ◽  
Optical Quality ◽  
Dye Lasers ◽  
Laser Dyes ◽  
Host Matrix ◽  
Host Matrices

AbstractPhotoresistant laser dyes were trapped in silica based xerogel host matrices to obtain solid state tunable lasers. For this purpose very dense xerogel samples with improved chemical and physical properties were prepared at room temperature by the sol-gel technology. The as-prepared materials were polished to obtain optical quality surfaces and were used as new lasing media.Lasing action of such different dyes as rhodamine, perylene and pyrromethene doping dense sol-gel matrices was demonstrated. Efficiencies of 30 % or lifetimes of more than 100,000 shots were achieved with different new ≤dye dopant/host matrix≥ couples. Their different performances are reviewed and discussed.

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