PEMANFAATAN DAUN MINT (Menta piperita) SEBAGAI ANTIMIKROBA ALAMI UNTUK MENGHAMBAT PERTUMBUHAN PATOGEN PADA JUS BUAH ALPUKAT

Previous research has identified the food safety aspects of the fresh fruit juices sold in Surakarta. The results showed that the level of microbiological contamination in fresh fruit juice was very high and it was not safe for consumption. This study aims to test the ability of mint leaves (Menta piperita) as a natural antimicrobial source to suppress the growth of pathogens in avocado juice. Mint leaves contain natural antimicrobial compounds in the form of menthol, menthone, menthophuran, and methyl acetate. The study used a factorial completely randomized design with 2 factors, namely the length of incubation time and the addition of mint leaves stored in standard room temperature and refrigerant. The incubation times were 0, 2, 4, and 6 hours. The results showed that mint leaves were able to suppress the number of Staphylococci, reaching the lowest number, namely 2.19 ± 0.00 log CFU / ml. Total Salmonella-Shigella was not detected by adding mint leaves and incubation for 6 hours at cold temperature. The total coliform count gave the final result with the lowest log colony count value in avocado juice and cold incubation temperature of 3.58 ± 0.43. The use of mint leaves as a natural antimicrobial in food is not yet effective, so it needs to be studied more about the use of its essential oil or needs to be explored for further effective methods.

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Suhu dan Waktu Inkubasi untuk Optimasi Kandungan Eksopolisakarida dan Fitohormon Inokulan Cair Azotobacter sp. LKM6

Jurnal Natur Indonesia ◽

10.31258/jnat.13.1.67-71 ◽

2012 ◽

Vol 13 (1) ◽

pp. 67

Author(s):

Reginawanti Hindersah ◽

Rija Sudirja

Keyword(s):

Heavy Metal ◽

Contaminated Soil ◽

Room Temperature ◽

Incubation Temperature ◽

Experimental Results ◽

Metal Mobility ◽

Randomized Design ◽

Completely Randomized Design ◽

Optimal Incubation

Azotobacter inoculation could play an important role to enhance the effectiveness of bioremediation since bacterialexopolysachharides form a complex with heavy metal. So that metal mobility in soil and its uptake by plantsincreased. Azotobacter also produce phytohormone which induce roots growth and subsequently the uptake ofnutrients. The objective of this research was to obtain optimal incubation temperature and time in Azotobacter sp.LKM6 liquid inoculants production in the fermentor to maximize the synthesis of exopolysachharides andphytohormones. The experiment arranged in Completely Randomized Design consisted of two incubationtemperature (room temperature and 300C). At 24, 36, and 48 hours incubation, the concentration of EPS andphytohormone cytokinin and giberrelin were occurred. The experimental results were 1) the best temperature andincubation time to produce Azotobacter sp. LKM6 liquid for bioremediation of heavy metal-contaminated soil was30oC and 48 hours, and 2) inoculants production at 300C for 48 jam produce liquid inoculants containing 2.87mg L-1 exopolysachharides, 81.0 mg L-1 cytokinins and 18.7 mg L-1 giberrelin, and 13.12 x 108 cell ml-1.

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The quality properties, thiobarbituric acid (TBA) values and microstructure of chicken sausage with local red beetroot powder

Food Research ◽

10.26656/fr.2017.5(s2).019 ◽

2021 ◽

Vol 5 (S2) ◽

pp. 113-119

Author(s):

W. Swastike ◽

E. Suryanto ◽

Rusman ◽

C. Hanim ◽

Jamhari ◽

...

Keyword(s):

Peroxide Value ◽

Room Temperature ◽

Thiobarbituric Acid ◽

Quality Properties ◽

Randomized Design ◽

Powder Addition ◽

Completely Randomized Design ◽

Different Levels ◽

Temperature Storage

This research was aimed to determine the quality properties, the microstructure of chicken sausage and Thiobarbituric acid (TBA) values with locally Indonesia red beetroot powder. The main ingredients of chicken sausage-making in this research were broiler chicken, filler, binder, beetroot powder, and spices. Red beetroot powder function as a filler was substituted tapioca starch in chicken sausage batter in three different levels. The combination of red beetroot powder with level 0, 1.0, 2.0 and 3.0% of total batter and shelf life at room temperature for 0, 1, 2 and 3 days. Each treatment consisted of five replications. The variables observed using quality properties (moisture, ash, fat, protein, crude fiber and calorie), microstructure and peroxide value of chicken sausage. The data of quality properties and peroxide value were analyzed by using one-way analysis (ANOVA) of Completely Randomized Design. The differences between means were analyzed by Duncan's New Multiple Ranges Test. The data of microstructure was analyzed by descriptive analyses. The moisture, protein, fat and ash contents for chicken sausages were significantly different (p<0.05). The chicken sausage with 2% substitution of beet powder produced chicken sausages with a high protein content of 14.77±0.02% while a low-fat content is 0.42±0.01%. Thiobarbituric acid (TBA) values of chicken sausages increased throughout the three days of room temperature storage (38°C). Chicken sausage formulated with red beetroot powder showed a significantly lower TBA value compared to the samples without red beetroot powder (p<0.05). In conclusion, a higher level of beetroot powder will improve the quality of chicken sausage and also the microstructure. The best level of beetroot powder addition was 2.0%. The addition of beetroot powder able to maintain fresh sausage conditions up to 2 days of storage at room temperature.

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Pertambahan Bobot Badan dan Waktu Pembusukan Daging Ayam Broiler yang Diberi Ekstrak Jaloh Dikombinasi dengan Kromium

Jurnal Agripet ◽

10.17969/agripet.v10i2.640 ◽

2010 ◽

Vol 10 (2) ◽

pp. 21-26

Author(s):

Sugito Sugito ◽

Erdiansyah Rahmi ◽

Azhari Azhari ◽

M. Isa

Keyword(s):

Drinking Water ◽

Body Weight ◽

Heat Stress ◽

Broiler Chickens ◽

Room Temperature ◽

The Body ◽

Feed Ratio ◽

Treatment Groups ◽

Randomized Design ◽

Completely Randomized Design

The gain body weight and Spoilage of meat broiler which giving jaloh extract to combine with chromiumABSTRACT. A study was conducted on broiler chickens under heat stress to evaluate the effect of combined jaloh (Salix tetrasperma Roxb) extract with chromium mineral in the body weight, value of feed ratio conversion, and time to meat to become decomposed. Twenty four of 20-day old Cobb female broiler chickens were randomly assigned and divided to 4 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens given no heat stress and jaloh extract nor chromium (tCp); 2) chickens under heat stress without given jaloh extract nor chromium (Cp); 3) chickens under heat stress and given jaloh extract in 1.000 mg/lt drinking water (Cp+Ej); and 4) chickens under heat stress, given jaloh extract in 1.000 mg/lt drinking water, and chromium in 1.000 ug/lt drinking water (Cp+Ej+Cr). Heat stress given was 33 ± 1oC of cage temperature during 4 hours per day within 15 days. Jaloh extract and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. On the 16th day of the study chickens were measured before slaughtered. Samples obtained were chicken meat that taken from breast part (musculus pectoralis). The result suggested that either giving jaloh extract per se at the dose of 1.000 mg/lt in drinking water or combine it with chromium that given two hours before cage temperature reaching 33 ± 1oC, can prevent chickens from decreasing their body weight, decreasing value of feed ratio convertion, and extending time to meat to become decomposed.

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THE EFFECT OF CORONA INCANDESCENT PLASMA RADIATION AND STORAGE TEMPERATURE ON THE VIABILITY OF GARLIC SEEDS (ALLIUM SATIVUM L. LUMBU KUNING VARIETY)

International Journal of Engineering Science Technologies ◽

10.29121/ijoest.v5.i1.2021.149 ◽

2021 ◽

Vol 5 (1) ◽

pp. 11-17

Author(s):

Febiasasti Trias Nugraheni ◽

Erma Prihastanti ◽

Endah Dwi Hastuti

Keyword(s):

Room Temperature ◽

Allium Sativum ◽

Storage Temperature ◽

Germination Percentage ◽

Plasma Radiation ◽

Agricultural Commodity ◽

Time Duration ◽

Randomized Design ◽

Completely Randomized Design ◽

And Storage

Garlic (Allium sativum L.) is an agricultural commodity which is widely consumed by the community. The obstacle experienced in cultivation is that a relatively long dormancy period of about 6-7 months. The dormancy of garlic seeds occurs because the embryo has not yet been fully formed, and the growth hormone and inhibitory hormone are not balanced. The methods used to break dormancy and accelerate germination are by using corona incandescent plasma radiation and storage temperature. The research utilized factorial Completely Randomized Design (CRD), consisting of six treatments with five replications. The first factor was the corona incandescent plasma radiation carried out on the garlic seeds ‘Lumbu Kuning’ variety with time duration of 0, 15, and 30 minutes. The second factor was storage temperature comprising of room temperature and cold temperature of 7oC stored for 30 days. Viability parameters observed are germination percentage dan the length of sprouts. The data were analyzed by using ANOVA followed by the DMRT test at the 95% confidence level. The results showed that corona incandescent plasma radiation influenced the germination percentage using plasma radiation for 15- 30 minutes.

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Postharvest of ‘Tommy Atkins’ Mango Submitted to Coating of Chlorella sp.

Journal of Agricultural Science ◽

10.5539/jas.v10n9p225 ◽

2018 ◽

Vol 10 (9) ◽

pp. 225 ◽

Cited By ~ 2

Author(s):

Agda M. F. de Oliveira ◽

Railene H. C. Rocha ◽

Welligthon A. Guedes ◽

George A. Dias ◽

José F. de Lima ◽

...

Keyword(s):

Food Safety ◽

Fruit Quality ◽

Room Temperature ◽

Design Experiment ◽

Consumer Health ◽

Mango Fruit ◽

Chlorella Sp ◽

Randomized Design ◽

Completely Randomized Design

The use of natural products as coatings to preserve the fruit quality during storage is an important step to maintain food safety for consumer health. The use of microalgae in coatings, therefore, may be promising in the preservation of mango. The present work had the objective to evaluate the effect of coatings based on Chlorella sp. on the postharvest preservation of ‘Tommy Atkins’ mango during storage at room temperature (23 °C). We carried out a completely randomized design experiment consisting of 0%, 1%, 2%, 3% and 4% of Chlorella sp., using 10 fruits per treatment (n = 10). Analyzing the L*, a* and, b* indices, in the peel and the pulp of the mango fruit, we observed a delay in the ripening with the increase of the biofilm concentration. The firmness of the pulp and maintenance of the organic acids of the fruits were higher in the treatments with a large amount of Chlorella sp. The use of biofilm with Chlorella sp. at 2% preserved the quality of ‘Tommy Atkins’ mango until ten days of storage, at 23 °C and 42% RH.

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THE EFFECTIVENESS OF PROTECTIVE POWER OF STARFRUIT (Averrhoa bilimbi) JUICE IN CATFISH AGAINST MOTILE AEROMONAS SEPTICEMIA DISEASES

Journal of Fish Health ◽

10.29303/jfh.v1i1.152 ◽

2021 ◽

Vol 1 (1) ◽

pp. 6-11

Author(s):

Sefti Heza Dwinanti ◽

Ratu Brata T. Mengkrin ◽

Ade Dwi Sasanti

Keyword(s):

Vitamin C ◽

Feed Efficiency ◽

Fruit Juice ◽

Challenge Test ◽

Feeding Duration ◽

Live Feed ◽

Star Fruit ◽

Randomized Design ◽

Completely Randomized Design ◽

And Control

Starfruit contains active ingredients such as flavonoids, phenols, antioxidants and vitamin C so that it can be used as an immunostimulant in catfish farming activities. Motile Aeromonas Septicemia (MAS) is one of the obstacles that can cause catfish farming losses. This study aims to determine the effect of differences in timing of feeding containing starfruit juice to protect catfish from MAS disease. The study was designed using a completely randomized design (CRD) consisting of 6 treatments (P) in the form of feeding duration containing starfruit for 7 days (P1), 14 days (P2), 21 days (P3), 28 days (P4). and 35 days (P5) and control of feeding without star fruit juice (P0). The research was carried out at the Aquaculture Laboratory, Sriwijya University. The dose of star fruit juice mixed in the feed is 300 mL.Kg-1 while the catfish used is 8-12 cm in size. The challenge test used Aeromonas hydrophilla bacteria with a density of 108 CFU.mL-1 which was injected as much as 0.5 mL.tail-1 intramuscularly. The results showed that administration containing starfruit juice for 28 days provided the best protection. Based on the hematological test the day before and before infection, the delta decreased the hematocrit by 3% and the delta decreased the erythrocyte count by 3,103 cells.mL-1. In addition, live feed after infection was 100%, prevalence was 17.78% and there was no effect of giving starfruit juice on growth and feed efficiency.

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Pengaruh Suhu Penyimpanan Terhadap Kerusakan Spora Myxobolus koi [The Effect of Storage Temperature in Myxobolus koi Spore Damage]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v11i1.12270 ◽

2019 ◽

Vol 11 (1) ◽

pp. 28

Author(s):

Gunanti Mahasri ◽

Titom Gusmana Putra Perdana ◽

Kusnoto Kusnoto

Keyword(s):

Data Analysis ◽

Main Parameter ◽

Room Temperature ◽

Storage Temperature ◽

Design Method ◽

Optimum Temperature ◽

Randomized Design ◽

Damage Data ◽

Different Temperatures ◽

Completely Randomized Design

AbstrakSpora Myxobolus koi dapat mengalami kerusakan apabila disimpan dalam kondisi penyimpanan yang kurang baik. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap kerusakan spora Myxobolus koi serta untuk mengetahui suhu optimum untuk penyimpanannya. Penelitian ini dilakukan dengan metode rancangan acak lengkap (RAL) sebagai rancangan percobaan. Perlakuan yang digunakan adalah penyimpanan spora Myxobolus koi pada suhu kamar (28-34) oC, Refrigerator (2-4) oC, dan Freezer (-5 hingga -10) oC, dengan ulangan sebanyak 6 kali. Penyimpanan ini dilakukan selama 30 hari. Parameter utama yang diamati adalah prosentase spora Myxobolus koi yang rusak. Parameter penunjang yang diamati adalah tipe kerusakan spora Myxobolus koi. Analisis data menggunakan ANAVA (Analisis Varian) dan dilanjutkan dengan Uji Jarak Berganda Duncan untuk mengetahui suhu optimum untuk penyimpanan spora Myxobolus koi. Berdasarkan hasil penelitian diketahui bahwa peyimpanan pada suhu yang berbeda berpengaruh terhadap prosentase kerusakan spora Myxobolus koi. Kerusakan spora Myxobolus koi tertinggi terjadi pada suhu kamar (28-34) oC mencapai angka 68,91% dan nilai kerusakan terrendah terjadi pada refrigerator (2-4) oC yaitu 29,91%. Spora Myxobolus koi dapat disimpan pada refrigerator dan lemari pembeku. AbstractMyxobolus koi spores can be damaged if stored in poor conditions. This study aimed to determine the effect of storage temperature on Myxobolus koi spores and to determine the optimum temperature for storage. This research was conducted using a completely randomized design method (CRD) as an experimental design. The treatments used were storaged Myxobolus koi spores at room temperature (28-34oC), Refrigerator (2-4oC), and Freezers (-5 to -10oC), with replications 6 times. This storage was carried out for 30 days. The main parameter observed was the percentage of damaged Myxobolus koi spores. The supporting parameters observed were the type of Myxobolus koi spore damage. Data analysis using ANAVA (Analysis of Variance) and continued with Duncan’s Multiple Distance Test to find out the optimum temperature for storage of Myxobolus koi spores. Based on the results of the study, it was found that storage at different temperatures affected the percentage of damage to Myxobolus koi spores. The highest damage of Myxobolus koi spores occurred at room temperature (28-34oC) reaching 68.91% and the lowest damage value occurred at refrigerator (2-4oC) which was 29.91%. Myxobolus koi spores can be stored in a refrigerator and freezer

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Cladode Pruning Affects Yield and Fruit Quality of ‘Roja Lisa’ Cactus Pear [Opuntia ficus-indica (L.) Mill.]: A Preliminary Study

Asian Plant Research Journal ◽

10.9734/aprj/2020/v6i430140 ◽

2020 ◽

pp. 86-90

Author(s):

Jorge A. Zegbe

Keyword(s):

Fruit Quality ◽

Water Loss ◽

Room Temperature ◽

Fruit Size ◽

Outer Part ◽

Single Plant ◽

Cactus Pear ◽

Flesh Firmness ◽

Randomized Design ◽

Completely Randomized Design

Aims: This study assessed the initial effect of experimental pruning (EP) or grower’s pruning (GP) of cactus pear fruiting cladodes on fruit yield (FY), fruit size distribution (FSD), and fruit quality (FQ) at harvest and after storage. Study Design: Treatments were arranged in a completely randomized design with eight or ten replicates, with a single plant as replicate, for EP or GP, respectively. Place and Duration of Study: The experiment was set up in a drip-irrigated commercial orchard of ‘Roja Lisa’ cactus pear [O. ficus-indica (L.) Mill.] plants located at Santa Fe, Jerez, Zacatecas, México, from February to August 2006. Methodology: The EP considered two agronomic practices: 1) exposing the center of each plant while eliminating unproductive cladodes and those shading other cladodes and 2) concentrating fruiting cladodes in the outer part of the plants. The GP randomly eliminated some cladodes from the central part and around the plants only. Treatments were arranged in a completely randomized design with eight or ten replicates, with a single plant as replicate, for EP or GP, respectively. The response variables were: FY, FSD, and FQ at harvest and after storage. The FQ attributes were: mean fruit mass (MFM), flesh firmness (FF), total soluble solids concentration, pulp and peel mass, dry matter concentration, and fruit water loss (FWL) during storage. Results: EP increased MFM by 42% over GP and produced 15% more marketable fruit (fruit equatorial diameter from 5.0 to 7.0 cm), but FY was reduced by 39%. The FF was higher in EP fruit than GP fruit after storage. The other FQ attributes were similar in both pruning treatments, both at harvest and after three weeks at room temperature. The FWL was also similar under both pruning systems. More targeted pruning has the potential to increase the productivity of cactus pear orchards. Conclusion: Experimental pruning increased fruit size and the percentage of commercial fruit, but reduced both overall and commercial fruit yields. After three-week storage at room temperature, flesh firmness remained greater in EP fruit. Fruit water loss was not influenced by pruning treatments during the storage.

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PENGARUH SUHU PENETASAN TERHADAP FERTILITAS, MORTALITAS DAN DAYA TETAS TELUR AYAM KAMPUNG (Gallus domesticus) PADA INKUBATOR

AGRIPTEK (Jurnal Agribisnis dan Peternakan) ◽

10.51673/agriptek.v1i1.611 ◽

2021 ◽

Vol 1 (1) ◽

pp. 23-28

Author(s):

Yuni Mariani ◽

M Ayatullah Hamzani

Keyword(s):

Data Analysis ◽

Analysis Of Variance ◽

Incubation Temperature ◽

Gallus Domesticus ◽

Free Range ◽

Randomized Design ◽

Native Chicken ◽

Significant Difference ◽

Completely Randomized Design ◽

Chicken Eggs

This study was intended to determine the best temperature for hatching free-range chicken eggs in the incubator so that breeders could get more day-old chicken (DOC) with the same diversity. The research design used a completely randomized design (CRD). This study used 150 free-range chicken eggs obtained from breeders in Gunung Paok Hamlet, Perian Village, Montong Gading District, East Lombok Regency, which were divided into three treatments, namely 35 ° C - 36 ° C, 37 ° C - 38 ° C and 39. ° C - 40 ° C and five repetitions with the number of eggs repeating using 10 free-range chicken eggs. The variables observed were fertility, mortality, and hatchability of native chicken eggs. Data analysis was performed using Analysis of Variance (ANOVA). The results of the analysis of variance showed that differences in the temperature of native chicken eggs in the incubator showed a significant difference (P> 0.05) in fertility, mortality, and hatchability. The best results were obtained at an incubation temperature of 37 ° C - 38 ° C with an average hatchability of 82,88± 8,87%.

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Studi Invitro Daun Alpukat (Persea americana Mill.) Untuk Pengobatan Sariawan

10.31227/osf.io/xuwyp ◽

2019 ◽

Author(s):

Rizki Rizki ◽

Yelsi Sepri Melda ◽

Mades Fifendy

Keyword(s):

Candida Albicans ◽

Saturated Fat ◽

Nutrient Content ◽

Persea Americana ◽

Randomized Design ◽

Avocado Fruit ◽

High Nutrient ◽

Completely Randomized Design ◽

Test Level ◽

Very High

Avocado (Persea americana Mill.) efficacious for treating various diseases. Avocado fruit has a very high nutrient content. Fruit contains 11 vitamins and 14 minerals that are beneficial. Avocados are rich in protein, riboflavin, niacin, potassium, essential oils, such as methylchavikol, alphapinene, tannins, and flavonoids. The pulp contains saturated fat, protein, sesqueterpenes, vitamin A, B1, and B2, and the leaves contain active compounds flavonoids, tannins, and quersetin. Candida albicans is a fungus that can cause various diseases, one sprue. Research has been conducted in order to determine the inhibition of leaf infusion avocado (Persea americana Mill.) on the growth of Candida albicans. This research was using a completely randomized design (CRD) with 6 treatments and 4 replications at each concentration infusion was 10%, 20%, 30%, 40%, 50%. Analysis of the data showed that treatment with each test level α f 5% significant and continued with HSD test. The results showed that 10% infusa avocado leaves (Persea americana Mill.) is able to inhibit the growth of Candida albicans.

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