scholarly journals 13. The Amount of Melanomacrophage centres (MMC) in Liver and Kidneys of Tilapia (Oreochromis niloticus) Maintained in Various Population Density

2017 ◽  
Vol 11 (2) ◽  
pp. 77-81
Author(s):  
Nurul Hadi ◽  
Dwina Aliza ◽  
Razali Daud
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Group Iv ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Liver And Kidney ◽  
Fish Group ◽  
Fish Kidney

The aim of this research was to find out the amount of melanomacrophage centres (MMC) in liver and kidney of nile tilapia (Oreochromis niloticus) fish reared in high dense population. Fifty nile tilapia were grouped in four treatment groups, group I contain 5 fish, group II contain 10 fish, group III contain 15 fish and group IV contain 20 fish. Treatment was carried out for 24 hours. Fish was necropsied to collect the liver and kidney, fixed in NBF 10% then proceed to histopathological method using hematoxilin and eosin for staining. Histopathological observation was done microscopically, and MMC was captured by photomicrograph. The data were analyzed descriptively. The result showed that the average of MMC number in fish liver in group K1, K2, K3, and K4 were 9.6, 11.3, 12.6, and 13.2. While the average of MMC number in fish kidney were 5.5, 7.7, 8.5, and 9.2, respectively. The higher the dense of population the higher the amount of MMC found in liver and kidney of nile tilapia.

Antioxidant potential of bulk and nanoparticles of naringenin against cadmium-induced oxidative stress in Nile tilapia, Oreochromis niloticus

2021 ◽  
Vol 10 (1) ◽  
pp. 392-402
Author(s):  
Nouf Abdallah Mreat Al-Ghamdi ◽  
Promy Virk ◽  
Awatif Hendi ◽  
Manal Awad ◽  
Mai Elobeid
Keyword(s):  
Oxidative Stress ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Negative Control ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Liver And Kidney ◽  
Hepatic Metallothionein

Abstract The study assessed the attenuating effect of citrus flavonone, naringenin, and its nanoformulation against cadmium (Cd) toxicity in Nile tilapia (Oreochromis niloticus). Nanoparticles of naringenin (NNn) were synthesized; size 165.1 nm (PDI 0.396) in variable shapes; cluster widespread, spherical accumulated, and tubular bacillary. Parallel, mature male Nile tilapia (n = 120 fish) were used for the exposure study. Group I was negative control. The groups II, III, and IV were exposed to 5 ppm of cadmium chloride monohydrate (CdCl2·H2O) for 21 days. Group III was treated with bulk naringenin (BNn) (3 ppm) and group IV was treated with NNn (3 ppm). Group V was exposed only to NNn (3 ppm). Cd-induced oxidative stress was evident from a significant increase in the hepatic malondialdehyde (MDA) levels and modulation of antioxidant enzymes in the liver and kidney. A significant increase in the hepatic metallothionein and HSP70 levels in the gills was observed. Treatment with both BNn and NNn significantly (p ≤ 0.05) reversed the Cd-induced alterations. However, the protective effect of nano naringenin was more profound on the bioaccumulation of Cd in liver and levels of HSP70 in the gills. These key findings could add to the commercial exploitation of naringenin and its nanosized form as a dietary therapeutic molecule.

scholarly journals Efficiency of eugenol as anesthetic for the early life stages of Nile tilapia (Oreochromis niloticus)

2015 ◽  
Vol 87 (1) ◽  
pp. 529-535 ◽  
Author(s):  
Paula A.P. Ribeiro ◽  
Kleber C. Miranda-Filho ◽  
Daniela C. de Melo ◽  
Ronald K. Luz
Keyword(s):  
Oreochromis Niloticus ◽  
Early Life ◽  
Nile Tilapia ◽  
Life Stages ◽  
Early Life Stages ◽  
Farmed Fish ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Nile Tilapia Oreochromis Niloticus

In aquaculture, activities with anesthetic compounds are usually used in order to ensure the welfare of farmed fish, allowing handling out of water with decreased trauma by stress. Presently, there is no information about anesthetic action of eugenol in early life stages of Nile tilapia (Oreochromis niloticus). The objective of this study was to evaluate different concentrations of eugenol for larvae and juveniles of Nile tilapia. Sixty animals were used for each group of weight, group I = 0.02 g; group II = 0.08 g; group III = 0.22 g; group IV = 2.62 g; and group V = 11.64 g. The eugenol concentrations tested were 50, 75, 100, 125, 150 and 175 mg L-1. No mortality was reported during the tests with eugenol. Tilapia larvae with 0.02 g and juveniles around 11.64 g can be anesthetized with eugenol concentrations between 150 and 175 mg L-1, since they determine the shortest sedation time (23 and 72 seconds, for the group of lowest and highest weights, respectively).

scholarly journals Pathomorphological changes of flunixin meglumine toxicity in layer chicks

2016 ◽  
Vol 8 (3) ◽  
pp. 1253-1259 ◽  
Author(s):  
R. A. Patel ◽  
K. B. Kapadiya ◽  
D. J. Ghodasara
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
Weight Ratio ◽  
Group Iv ◽  
Mean Values ◽  
Group Iii ◽  
Treatment Groups ◽  
Flunixin Meglumine ◽  
Group I ◽  
Urate Crystals

The aim of the 21 day toxicity study was to evaluate the pathomorphological effect of flunixin meglumine in layer chicks. The chicks of Group I were kept as control while groups II, III and IV were fed with diet containing flunixin meglumine @ 10 ppm, 25 ppm and 50 ppm respectively for 21 days. Clinical signs viz. anorexia, dullness,lethargy, lameness and uneven growth were noticed in chicks of treatment groups III and IV only. Maximum mortality was observed in group IV (12%) followed by group III (4%). A dose dependant reduction in body weight was observed in all the treatment groups. The mean values of Kidney: Body weight ratio was significantly increased in group IV. The plasma uric acid, creatinine and BUN values were significantly increased in group III whereas increase in group IV was highly significant. Grossly, there was deposition of chalky white urates on serosal surface of kidney, heart and liver in chicks of group IV which died during experiment. Microscopically, lesions were characterized by varying degrees of congestion, haemorrhages, degeneration, necrosis and deposition of urate crystals in visceral organs of group III and group IV chicks. The intensity and distribution of pathological lesions were more severe in chicks of group IV, followed by chicks of group III. The overall lesions gave an impression that flunixin meglumine was nephrotoxic in nature.

Oxidative stress in gill, erythrocytes, liver and kidney of Nile tilapia (Oreochromis niloticus) from a polluted site

1996 ◽  
Vol 34 (2) ◽  
pp. 151-162 ◽  
Author(s):  
Afonso C.D. Bainy ◽  
Erika Saito ◽  
Paulo S.M. Carvalho ◽  
Virgínia B.C. Junqueira
Keyword(s):  
Oxidative Stress ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Polluted Site ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Liver And Kidney

scholarly journals Comparative Study of Serum Profile in Bitches Treated for Transmissible Venereal Tumor by Autohaemotherapy

2021 ◽  
Vol 10 (11) ◽  
pp. 19-22
Author(s):  
C. K. Lakde A. D. Patil ◽  
S. K. Sahatpure A. P. Gawande
Keyword(s):  
Side Effects ◽  
Point Of View ◽  
Vaginal Examination ◽  
Kidney Function Tests ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Vincristine Sulphate ◽  
Vaginal Cytology ◽  
Liver And Kidney

A total of 18 bitches in the period in the period of six month diagnosed for transmissible venereal tumor in Teaching Veterinary Clinical Complex, Nagpur by per vaginal examination and vaginal cytology. These bitches were divided randomly into three groups, viz., Group I (Inj. Vincristine @ 0.025mg/kg slow IV in DNS and Injection Botropase 1ml IM for 1st week), Group II (Inj. Vincristine @ 0.025mg/kg slow IV in DNS and Tab. Amoxycillin and Potassium Clavinate for first 7 days orally) and Group III (treated with autohaemotherapy@10 ml whole blood IM) for once in week and continue for 5 week. At the time of treatment, liver and kidney function tests were performed. During the course of treatment significant variation was noted in Group I and II in BUN values while creatinine values changes significantly in all treatment groups. In case of SGPT only significant changes observed in Group I. Rest of the changes in serum prolife found to be non significant. Also from recovery point of view we noted that, it was faster in Group I and II compare than Group III. Side effects observed only in Group I and II. From the study we concluded that autohaemotherapy will be an alternate option for treatment of TVT in bitches who shows the excessive side effects of Vincristine Sulphate.

scholarly journals Chemotherapeutic Efficacy of Indigofera aspalathoides on 20-Methylcholanthrene-Induced Fibrosarcoma in Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Selva Kumar Sivagnanam ◽  
Mudiganti Ram Krishna Rao ◽  
Maruthaiveeran Periyasamy Balasubramanian
Keyword(s):  
Sialic Acid ◽  
Aqueous Extract ◽  
Group Iv ◽  
Control Group ◽  
Albino Rats ◽  
Activity Levels ◽  
Aqueous Extracts ◽  
Group Iii ◽  
Group I ◽  
Liver And Kidney

The present study was undertaken to test the chemopreventive effects of one herbal medicinal plant, Indigofera aspalathoides, on chemically induced carcinogenesis in rats. A well-known polyaromatic hydrocarbon, namely, 20-methylcholanthrene, which is a known carcinogenic substance, was used to induce fibrosarcoma in Wistar strain of male albino rats. Fibrosarcoma rats were treated with aqueous extracts of Indigofera aspalathoides. The rats were divided into four groups, each consisting of six animals. Group I served as normal control, Group II served as fibrosarcoma-induced animals, Group III were fibrosarcoma-bearing animals treated with aqueous extracts of Indigofera aspalathoides, and Group IV animals, which were normal healthy animals treated with Indigofera aspalathoides aqueous extract, served as drug control set. Group III and Group IV animals were treated with aqueous extract of Indigofera aspalathoides intraperitoneally at a dose of 250 mg/kg. b.w. for 30 days. The fibrosarcoma was proved by pathological examinations. The activity levels of nucleic acids such as total DNA and RNA and hexose, hexosamine, and sialic acid in liver and kidney of treated rats were used to monitor the chemopreventive role of the plant extract. The observed increase in the levels of DNA, RNA, hexose, hexosamine, and sialic acid in liver and kidney tissues of fibrosarcoma-bearing animals reached near normal state after the treatment with aqueous extracts of Indigofera aspalathoides, suggesting that Indigofera aspalathoides does have a chemotherapeutic role.

scholarly journals Economic feasibility of probiotic use in the diet of Nile tilapia, Oreochromis niloticus, during the reproductive period

2020 ◽  
Vol 42 ◽  
pp. e47960
Author(s):  
Danielle de Carla Dias ◽  
Fernanda de Paiva Badiz Furlaneto ◽  
Fábio Rosa Sussel ◽  
Leonardo Tachibana ◽  
Giovani Sampaio Gonçalves ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Egg Production ◽  
Reproductive Period ◽  
Economic Feasibility ◽  
Feed Additive ◽  
Total Cost ◽  
Treatment Groups ◽  
Nile Tilapia Oreochromis Niloticus

This work examines the economic advantages of probiotic use in the diet of Nile tilapia broodstock during the reproductive period. For this purpose, Bacillus subtilis was applied as a feed additive. The experimental design was completely randomized with three treatment groups: the T0 control (without probiotic), the T1 continuous probiotic intake, and the T2 alternate probiotic intake at a dose of 0.50 g kg-1 of feed (1010 CFU g-1) with four replicates. For the reproduction assay, 118 females and 48 males of Nile tilapia (proportion 4 males:9 females. hapa-1) (weight 527.65 g ± 185.98 g and length 30.16 cm ± 3.57 cm) were distributed into 12 hapas (3.5 × 2.0 × 1.5 m). Reproductive variables (spawning female percentage, egg production, and fry production) were used to calculate the economic feasibility indexes (total cost of nutrition [TcN], gross revenue [GR], and total operational profit [ToP]). The results show increasing values for spawning female number, collected eggs, and surviving fry in the probiotic groups. We recommend continuous intake of probiotic (feed with addition of probiotic) at a dose of 0.5 g kg-1 of feed (1010 CFU g-1) during the breeding season of Nile tilapia, due to the suitable reproductive indexes and profitability.

scholarly journals Behavior and Gill profile of Nile Tilapia (Oreochromis niloticus) Induced by Density stress

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Dwinna Aliza
Keyword(s):  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Control Group ◽  
Tissue Samples ◽  
Group I ◽  
Secondary Lamella ◽  
Fish Group ◽  
Hematoxylin And Eosin ◽  
Group Ii ◽  
Anatomical Pathology

 The aim of this research was to observe behavior and gill profile of nile tilaphia (Oreochromis niloticus) induced by density stress. A total of 50 nile tilapia fish with the age of 3-8 months and 80-100 bwwere divided into 4 groups. Group I was control group contained 5 fish, group II,III, and IV contained 10, 15, and 20 fish, respectively. Treatment was performed for 24 hours, and fish behavior was observed every 3 hours. Gillsample was examined by anatomical pathology then tissue samples were collected and fixed in 10% Davidson solution, routinely processed,embedded in paraffin, sectioned at 5 microns, stained with hematoxylin and eosin (HE), and examined by light microscopy. The data wasanalyzed descriptively. Behavior changessuch as swim up to water surface and rapid movement of operculum occur earlier than passivemovement and decreased reflex of fish on group II, III, and IV. The anatomical pathology of nile tilapia gills observed were darker colour ofgills, gill rott, and over mucus.Histopathological result showed that haematoma, necrosis, hyperplasia of primary and secondary lamella, andfusion lamella were observed in gills of nile tilapia induced by density stress.____________________________________________________________________________________________________________________Key words: histopathology, nile tilapia, gill, density stress 

scholarly journals The Efficacy Study of Duramectin, Oxfendazole, Piperazine, and Pyrantel pamoate Against Gastrointestinal Worms In Horses In Yogyakarta Special Region*)

2019 ◽  
Vol 37 (1) ◽  
pp. 112
Author(s):  
Yuriadi Yuriadi ◽  
Ida Tjahajati ◽  
Soedarmanto Indarjulianto ◽  
Irkham Widiyono
Keyword(s):  
Body Weight ◽  
Clinical Symptoms ◽  
Research Result ◽  
Group Iv ◽  
Weight Group ◽  
Group Iii ◽  
Treatment Groups ◽  
Central Java ◽  
Group I ◽  
Special Region

ABSTRACT This research aimed at determining the efficacy of duramectin, oxfendazole, piperazine, and pyrantelpamoate against gastrointestinal worms in horses in Yogyakarta Special Region and Central Java. The object of research involved 40 horses diagnosed with gastrointestinal worm infection. Prior to the research, all of the horses were subjected to examination for clinical symptoms and parasitology laboratory checkup for signs of worm eggs in their feces, and they were pronounced positive for experiment animals with at least 150 eggs per gram of feces per horse. The research horses were weighed to determine the dose of worm medication to be used. The research horses are divided into four treatment groups, each group consists of ten horses. Group I was given duramectin with a dose of 0.2 mg/kg of body weight. Group II was given oxfendazole with a dose of 7.5 mg/kg of body weight, Group III was given 125 mg Piperazin treatment per kilogram of body weight and Group IV was given pyrantelpamoate with a dose of 20 mg/kg of body weight. After receiving themedication, the horses were observed to document the progress in terms of clinical symptoms and the amount and type of worm eggs in their feces every three days for four times in a row. The research data results were tabulated and were descriptive-comparatively analyzed.The research result showed varying efficacy level of worm medication for horses. Oxfendazole kills Strongylus and Parascaris worms. Duramectin kills Strongylus worms and reduce some of the Parascaris worms. Piperazineandpyrantelpamoate kills the Strongylus wormsand reduce some of the Parascaris. Keywords: horses, gastrointestinal worms, Duramectin, Oxfendazole, Piperazine, PyrantelPamoate.  

Sign in / Sign up

Export Citation Format

Share Document