scholarly journals 14. The Potency of Sernai (Wedelia biflora) Leaf N-hexan Extract as Analgesic Compared to Ibuprofen on Mice (Mus musculus)

2018 ◽  
Vol 12 (2) ◽  
pp. 84-90
Author(s):  
Zulkarnaini Zulkarnaini ◽  
Rinidar Rinidar ◽  
Rosmaidar Rosmaidar ◽  
T. Armansyah TR ◽  
Abdul Harris ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Hot Plate ◽  
Plate Method ◽  
Treatment Groups ◽  
Significant Difference ◽  
Plot Design ◽  
Leaf N

This study aimed to determine the analgesic potential of sernai leaves n-Hexan extract on 24 male mice, aged 2-3 month with 20-25 g weighed. This study used split-plot design consisted of 6 groups. P0 as negative control was given aquades and P1 as positive control was given ibuprofen (10 mg/kg Body Weight). Treatment groups including P2, P3, P4, and P5 were given n-Hexan extract of sernai leaves with dosage of 15, 30, 45, and 60 mg/kgBW, respectively. Pain assessment was conducted using Hot Plate method. Observation of mice behaviour including licking it's front and back leg, or by jumping. This study showed there was significant difference (P0,05) between dosage and time. Duncan’s test showed P1 was significantly different (P0,05) from P2, but P3 and P5 were not significantly different from P1. P3 and P4 were significantly different (P0,05) from P2 and not significantly different from P1, while P5 was not significantly different from P3 and significantly different from P1. We can conclude that dosage of 30 and 45 mg/kgBW of n-Hexan extract of sernai leaves had analgesic potential on mice, and 60 mg/kgBW was comparable to ibuprofen as pain relieve in mice

scholarly journals 18. POTENSI EKSTRAK AIR DAUN SERNAI (Wedelia biflora) SEBAGAI ANTINYERI PADA MENCIT (Mus musculus) The Potency of Wedelia biflora Leaf Water Extract as Pain killer on Mice (Mus musculus)

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Abdul Hayi Amarta Ali
Keyword(s):  
Mus Musculus ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Hot Plate ◽  
Plate Method ◽  
Treatment Groups ◽  
Randomized Design ◽  
Group 2 ◽  
Analgesic Potency

The purpose of this research was to find out the potency of Wedelia biflora water extract as pain killer. Completely randomized design (CDR) was used in this study. A total of 30 mice (Mus musculus) were divided into five treatment groups. Mice in group 1 (P0) were administered with mefenamat (positive control) and mice group 2 (P1) were only given aquadest (negative control). Mice in another3 groups (P2, P3, and P4) were administered with Wedelia biflora water extract with the concentration of 100, 75, and 50%, respectively. The painful response time was done using hot plate method and observed at 0, 30, 60, and 90 minutes, whereas abdominal writhing method was applied to count the total of writhing. Data were analyzed using ANOVA followed by Duncan. Result showed that Wedelia biflora water extract with various levels of concentration were affect significantly (P0.05) compared to negative control but no significant different (P0.05) with positive control. The analgesic potency showed significant effect (P0.05) at concentration of 100 and 75% compared to negative control, but no significant different (P0.05) compare to positive control on both hot plate methods and abdominal writhing method, whereas 50% concentration showed analgesic potency (P0.05) only on hot plate method. In conclusion, Wedelia biflora water extract is potential as analgesic on mouse and shows no significant different with mefenamat. ____________________________________________________________________________________________________________________ Key words: analgesik, Wedelia biflora, painful, total of writhe

scholarly journals The Use of Torbangun Leaf Powder (Coleus amboinicus L) in Increasing Prolactin in Breastfeedings Wistar Rats and Bodyweight of the Litters

2021 ◽  
Vol 3 (1) ◽  
pp. 26-33
Author(s):  
Stella T Hasianna ◽  
July Ivone ◽  
Sifa D Shinta ◽  
R Zahra Nadhira
Keyword(s):  
Body Weight ◽  
Breast Milk ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Breast Milk Production ◽  
Significant Difference ◽  
Leaf Powder ◽  
Coleus Amboinicus

Research on local herbs that have the effect of increasing breast milk production isneeded to help mothers with insufficient breastmilk. This research was conducted to determinethe effect of torbangun (Coleus amboinicus L) leaf powder in increasing prolactin inbreastfeeding Wistar rats and the bodyweight of the litters. Thirty breastfeeding Wistar rats weredivided into 5 groups with 5 litters each. The treatments given were 108 mg, 216 mg, and 432 mgtorbangun leaf powder (TLP), with domperidone as the positive control, and CMC 10% as thenegative control. The treatment was given 3 times/day for 14 days. Tukey's HSD means testshowed a significant difference in prolactin levels between the negative control (NC), TLP1, andTLP2 groups and the TLP3 and positive control (PC) groups with all of them having p = 0.000.There was no difference in the increase in body weight of litters between treatment groups (p =0.866). In conclusion, torbangun leaves increase the prolactin levels in breastfeeding rats buthave no difference in the increase in body weight of litters between treatment groups. Keywords: torbangun leaves; prolactin; bodyweight; breastfeeding

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

scholarly journals KULIT BUAH JERUK LIMAU (Citrus amblycarpa (Hassk.) Osche) SEBAGAI ANALGESIK

Jurnal Kimia ◽  
2020 ◽  
pp. 24
Author(s):  
R. A. I. K. Maharani ◽  
N. K. Cahyaningsih ◽  
M. D. Abimanyu ◽  
K. W. Astuti
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Treatment Options ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Fruit Peel ◽  
Hot Plate ◽  
Inflammatory Drugs ◽  
Negative Controls

Nonsteroidal anti-inflammatory drugs (NSAIDs) are the treatment options for relieving pain. However, long-term use can trigger gastrointestinal bleeding. Therefore, alternative analgesics which have the same therapeutic effect with lower side effects are needed. Limau (Citrus amblycarpa) is an empirical drug for tingling and cramping. The aim of the study is to determine the analgesic activity of ethanol extract of C. amblycarpa fruit peel. The method used in testing analgesic activity is the Hot Plate method. The study was conducted by dividing 30 mice into 6 groups. The group given CMC-Na 1% was used as a negative control, the group given suspension of sodium diclofenac dose of 6.5 mg/kg of body weight was used as a positive control, and the group given suspension of ethanol extract of C. amblycarpa fruit peel with dose variations 100, 300 and 600 mg/kg of body weight. The test animals were placed on top of the Hot Plate with a temperature of 70°C at 30 minutes after giving suspension test and the response time of mice to heat was observed every 30 minutes for 3 hours with cut off time 15 second. Based on the test results, it can be concluded that the administration of ethanol extract of C. amblycarpa fruit peel with 100, 300 and 600 mg/kg of body weight gave analgesic activity on mice compared to the negative controls (CMC-Na 1%).   Keywords: C. amblycarpa, Fruit Peel, Analgesics, Hot Plate 

scholarly journals 2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nada Sarah Syahputri ◽  
Nuzul Asmilia ◽  
Rinidar Rinidar ◽  
Amalia Sutriana ◽  
Fakhrurrazi Fakhrurrazi ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Positive Control ◽  
Negative Control ◽  
Hexane Extract ◽  
Phyllanthus Emblica ◽  
Treatment Groups ◽  
Significant Difference ◽  
Treatment Data ◽  
The Mean

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo

scholarly journals Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

2021 ◽  
Vol 4 (2) ◽  
pp. 73
Author(s):  
Putri Ayu Ika Setiyowati ◽  
Rofiatun Solekha ◽  
Sri Bintang Sahara Mahaputra Kusuma Negara ◽  
Reny Rosalina
Keyword(s):  
Body Weight ◽  
Optimal Dose ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Optimal Dosage ◽  
Skin Injury ◽  
Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

The enhancement of Arg1 and activated ERβ expression in microglia HMC3 by induction of 96% ethanol extract of Marsilea crenata Presl. leaves

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Burhan Ma’arif ◽  
Mangestuti Agil ◽  
Hening Laswati
Keyword(s):  
Laser Scanning ◽  
Negative Relationship ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Scanning Microscope ◽  
Treatment Groups ◽  
Arginase 1 ◽  
Significant Difference ◽  
Ifn Γ

AbstractBackgroundPhytoestrogens have a high potential to overcome the neuroinflammation caused by estrogen deficiency. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to report the activity of a 96% ethanol extract of M. crenata leaves in inducing activation of microglia HMC3 cell to M2 polarity, which has anti-inflammatory characteristics.MethodsThe study was done by culturing microglia HMC3 cell in 24-well microplate and inducing it with IFN-γ for 24 h to activate the cell to M1 polarity, which has proinflammatory characteristics. The 96% ethanol extract was added with various doses of 62.5, 125, and 250 ppm. Genistein, 50 μM, was used as a positive control. The analysis of the immunofluorescence of Arginase-1 (Arg1) and ERβ as markers was done using a convocal laser scanning microscope.ResultsThe result of Arg1 shows a significant difference in Arg1 expression in the microglia HMC3 cell line between the negative control and all treatment groups at p < 0.05, with the best result at 250 ppm, whereas for ERβ, the results show, at doses of 125 and 250 ppm, that the 96% ethanol extract of M. crenata leaves decrease the activated ERβ expression at p < 0.05, with the best result at 250 ppm. The Arg1 and activated ERβ expression have a weak negative relationship with the Pearson correlation test.ConclusionsThe 96% ethanol extract of M. crenata leaves has an antineuroinflammation activity through the induction of Arg1 and activated ERβ expression in microglia HMC3 cell, with the best dose at 250 ppm.

scholarly journals The Potential of Billygoat Weed Leaf Extract (Ageratum Conyzoides) in Increasing Platelet Counts of Male Mice (Mus Musculus)

2020 ◽  
Vol 11 (1) ◽  
pp. 51-57
Author(s):  
Yessy Velina ◽  
Nirva Diana ◽  
Aulia Annisa
Keyword(s):  
Platelet Count ◽  
Positive Control ◽  
Negative Control ◽  
Plain Water ◽  
P Value ◽  
Male Mice ◽  
Ageratum Conyzoides ◽  
Platelet Counts ◽  
Randomized Design ◽  
Significant Difference

Billygoat weed (Ageratum conyzoides) is mainly used to treat wounds and fever. People also do not understand the use of these leaves in the treatment to increase the platelet counts. The objectives of this research Objectives were to test whether the Billygoat Weed’s leaves can increase platelet count of male mice and to find out what is the most effective dose of the Billygoat Weed on the platelet count of male mice. This research was done by employing the true experimental approach with the completely randomized design which consisted of 5 treatments, namely the negative control (plain water), the positive control (acetylsalicylic acid), 1.68 mg/kgBW, 2.52 mg/kgBW, and 3.36 mg/kgBW on five mice in each treatment. The increase in the platelet counts, based on the results of One-Way ANOVA with a p-value of 0,0004, the Billygoat Weed extract was able to increase the platelet counts. Through further testing, the smallest significant difference showed that a dose of 3.36 mg/kgBW was effectively able to increase the platelet counts of male mice with an average of 11140.80/. Billygoat weed (Ageratum conyzoides) is mainly used to treat wounds and fever. People also do not understand the use of these leaves in the treatment to increase the platelet counts. The objectives of this research Objectives were to test whether the Billygoat Weed’s leaves can increase platelet count of male mice and to find out what is the most effective dose of the Billygoat Weed on the platelet count of male mice. This research was done by employing the true experimental approach with the completely randomized design which consisted of 5 treatments, namely the negative control (plain water), the positive control (acetylsalicylic acid), 1.68 mg/kgBW, 2.52 mg/kgBW, and 3.36 mg/kgBW on five mice in each treatment. The increase in the platelet counts, based on the results of One-Way ANOVA with a p-value of 0,0004, the Billygoat Weed extract was able to increase the platelet counts. Through further testing, the smallest significant difference showed that a dose of 3.36 mg/kgBW was effectively able to increase the platelet counts of male mice with an average of 11140.80/𝑚𝑚3.

scholarly journals Anti-plasmodial Effect of C. limon and C. paradisi extracts on Plasmodium berghei-infected mice

2021 ◽  
Vol 8 (2) ◽  
pp. 026-033
Author(s):  
Michael Okpara Elom ◽  
Anthony Gideon Uche ◽  
Boniface Nwofoke Ukwah ◽  
Victor Udoh Usanga ◽  
Anthonia Ifeoma Okpara-Elom ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Combined Therapy ◽  
Parasite Clearance ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Citrus Limon ◽  
Citrus Paradisi ◽  
Treatment Groups ◽  
Significant Difference

Antiplasmodial effect of Citrus limon and Citrus paradisi extracts on Plasmodium berghei-infected mice was studied. Twenty five albino mice were randomized into five categories of G, L, GL, ACT (positive control) and NC which stand for grape, lemon, grape and lemon combined extracts, artemisinin combined therapy and negative control respectively. The NC group did not receive any intervention. Other treatments were administered orally for 12 days whereas administration of ACT lasted for 3 days. Blood was collected from the tail vein of the mice at a three day interval through venipuncture. Thick blood films were prepared and parasite densities were estimated using standard parasitological techniques. Results were analysed with ANOVA and Duncan multiple range tests. There was no significant difference (p>0.05) between parasite densities of the treatment groups and the negative control at baseline levels. However, as the treatment progressed from day 3 through day 9, there were significant reductions (p<0.05) in parasite densities among treatment groups when compared to the negative control. In this study, extracts of C. limon and C. paradisi in both single and combined strengths have been found to have antiplasmodial properties in mice. ACT possessed the highest antiplasmodial effect while C. limon as a single treatment ranked second in possession of antiplasmodial activity but exhibited increased RBC lysis. In combination, C. limoni and C. paradise extracts showed antiplasmodial activity that is slightly less than that exhibited by the lemon extract alone, but maintained normal RBC morphology whereas C. paradisi extract alone exhibited the lowest level of parasite clearance with atrophied red blood cells. Investigation of the effects of the extracts on liver, kidney and gastrointestinal tissues of mice is recommended before they could be prescribed as antimalaria for other animals and humans.

scholarly journals Pengaruh Pemberian Kombinasi Ekstrak Etanol Daun Sidaguri (Sida rhombifolia L.) dan Rimpang Jahe Merah (Zingiber officinale Rosc.) pada Mencit Jantan Hiperurisemia

2018 ◽  
Vol 6 (2) ◽  
pp. 205
Author(s):  
Kinanthi Putri Rizki ◽  
Siti Muslichah ◽  
Indah Yulia Ningsih
Keyword(s):  
Uric Acid ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Treatment Groups ◽  
Ginger Rhizome ◽  
Red Ginger ◽  
Sida Rhombifolia ◽  
Potassium Oxonate

  This study aimed to determine the effect of the combination of sidaguri leaves and red ginger rhizome extracts in uric acid levels of hyperuricemic male mice. Thirty-six mice were divided into nine groups including normal group, the positive control (+), negative control (-), extracts of four treatment groups with a variety of combinations and two groups of single extract. Hyperuricemia induction was performed by administering mixture melinjo 10% of the standard feed mice for 7 days dan potassium oxonate 250 mg/kg BB intraperitoneally at 2 hours before blood sampling. The results showed that the combination dose of sidaguri leaves of 50 mg/kg BB and red ginger rhizome 400 mg/kg BB had higher antihyperuricemia activity than other combinations in lowering uric acid levels of mice. Compounds may have antihyperuricemic activity were flavonoids, alkaloids, saponins, tannins, and terpenoids.   Keywords: sidaguri leaves, red ginger rhizome, antihyperuricemic activity, uric acid  

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