scholarly journals Overexpression of GmCAMTA12 enhanced drought tolerance in Arabidopsis and Soybean

2019 ◽  
Author(s):  
Muhammad Noman ◽  
Aysha Jameel ◽  
Weidong Qiang ◽  
Naveed Ahmad ◽  
Liu Weican ◽  
...  
Keyword(s):  
Drought Stress ◽  
Hairy Roots ◽  
Interaction Network ◽  
Average Survival ◽  
Overexpression Line ◽  
Physiological Indexes ◽  
Functional Analyses ◽  
Upregulated Genes ◽  
Molecular Measures ◽  
Average Survival Rate

Abstract Background: Earlier, fifteen transcription factors in the CAMTA family of soybean were reported to differentially express in multiple stresses however, their functional analyses had not yet been attempted. To characterize their role in drought stress, we comprehensively analyzed GmCAMTA family in silico and determined their expression pattern after which we cloned and overexpressed the 2769 bp CDS of GmCAMTA12 in Arabidopsis and soybean and carried out drought assays. Results: The bioinformatics analysis revealed multiple stress-related cis motifs including ABRE, SARE, G-box and W-box, 10 unique miRNA targets in GmCAMTA transcripts and 48 proteins in GmCAMTAs’ interaction network. The stably transformed homozygous lines of Arabidopsis overexpressing GmCAMTA12 exhibited enhanced tolerance to drought in soil as well as on MS media containing mannitol. In their drought assay, the average survival rate of transgenic Arabidopsis lines OE5 and OE12 (Overexpression Line 5 and Line 12) was 83.66% and 87.87% respectively, which was ~30% higher than that of wt. In addition, the germination and root length assays as well as physiological indexes such as proline and MDA contents, CAT activity and leakage of electrolytes affirmed the better performance of OE lines. Likewise, GmCAMTA12 overexpression in soybean promoted more developed hairy roots in OE chimeric plants as compare to that of VC (Vector control). In parallel, the improved growth performance of OE in Hoagland-PEG and on MS-mannitol was revealed by their phenotypic, physiological and molecular measures. Furthermore, with the overexpression of GmCAMTA12, the downstream genes including AtAnnexin5, AtCaMHSP, At2G433110 and AtWRKY14 were upregulated in Arabidopsis. Likewise, in soybean hairy roots, GmELO, GmNAB and GmPLA1-IId were significantly upregulated as a result of GmCAMTA12 overexpression and majority of these upregulated genes in both plants possess CAMTA binding CGCG/CGTG motif in their promoters. Conclusions: Taken together, we report that GmCAMTA12 plays substantial role in tolerance of soybean against drought stress and could prove as a novel candidate for engineering of soybean and other plants against drought stress. Some research gaps were also identified for future studies to extend our comprehension of Ca-CaM-CAMTA-mediated stress regulatory mechanisms.

scholarly journals Overexpression of GmCAMTA12 Enhanced Drought Tolerance in Arabidopsis and Soybean

2019 ◽  
Vol 20 (19) ◽  
pp. 4849 ◽  
Author(s):  
Muhammad Noman ◽  
Aysha Jameel ◽  
Wei-Dong Qiang ◽  
Naveed Ahmad ◽  
Wei-Can Liu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Hairy Roots ◽  
Interaction Network ◽  
Regulatory Elements ◽  
Transcription Activator ◽  
Calmodulin Binding ◽  
Average Survival ◽  
Overexpression Line ◽  
Functional Analyses ◽  
Upregulated Genes

Fifteen transcription factors in the CAMTA (calmodulin binding transcription activator) family of soybean were reported to differentially regulate in multiple stresses; however, their functional analyses had not yet been attempted. To characterize their role in stresses, we first comprehensively analyzed the GmCAMTA family in silico and thereafter determined their expression pattern under drought. The bioinformatics analysis revealed multiple stress-related cis-regulatory elements including ABRE, SARE, G-box and W-box, 10 unique miRNA (microRNA) targets in GmCAMTA transcripts and 48 proteins in GmCAMTAs’ interaction network. We then cloned the 2769 bp CDS (coding sequence) of GmCAMTA12 in an expression vector and overexpressed in soybean and Arabidopsis through Agrobacterium-mediated transformation. The T3 (Transgenic generation 3) stably transformed homozygous lines of Arabidopsis exhibited enhanced tolerance to drought in soil as well as on MS (Murashige and Skoog) media containing mannitol. In their drought assay, the average survival rate of transgenic Arabidopsis lines OE5 and OE12 (Overexpression Line 5 and Line 12) was 83.66% and 87.87%, respectively, which was ~30% higher than that of wild type. In addition, the germination and root length assays as well as physiological indexes such as proline and malondialdehyde contents, catalase activity and leakage of electrolytes affirmed the better performance of OE lines. Similarly, GmCAMTA12 overexpression in soybean promoted drought-efficient hairy roots in OE chimeric plants as compare to that of VC (Vector control). In parallel, the improved growth performance of OE in Hoagland-PEG (polyethylene glycol) and on MS-mannitol was revealed by their phenotypic, physiological and molecular measures. Furthermore, with the overexpression of GmCAMTA12, the downstream genes including AtAnnexin5, AtCaMHSP, At2G433110 and AtWRKY14 were upregulated in Arabidopsis. Likewise, in soybean hairy roots, GmELO, GmNAB and GmPLA1-IId were significantly upregulated as a result of GmCAMTA12 overexpression and majority of these upregulated genes in both plants possess CAMTA binding CGCG/CGTG motif in their promoters. Taken together, we report that GmCAMTA12 plays substantial role in tolerance of soybean against drought stress and could prove to be a novel candidate for engineering soybean and other plants against drought stress. Some research gaps were also identified for future studies to extend our comprehension of Ca-CaM-CAMTA-mediated stress regulatory mechanisms.

Bioinformatics Analysis Reveals Functions of MicroRNAs in Rice Under the Drought Stress

2021 ◽  
Vol 15 (8) ◽  
pp. 927-936 ◽  
Author(s):  
Yan Peng ◽  
Yuewu Liu ◽  
Xinbo Chen
Keyword(s):  
Drought Stress ◽  
Target Genes ◽  
Hot Spot ◽  
Interaction Network ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Protein Protein Interaction ◽  
Promising Tool ◽  
Differentially Expressed Mirnas ◽  
Aba Biosynthesis

Background: Drought is one of the most damaging and widespread abiotic stresses that can severely limit the rice production. MicroRNAs (miRNAs) act as a promising tool for improving the drought tolerance of rice and have become a hot spot in recent years. Objective: In order to further extend the understanding of miRNAs, the functions of miRNAs in rice under drought stress are analyzed by bioinformatics. Method: In this study, we integrated miRNAs and genes transcriptome data of rice under the drought stress. Some bioinformatics methods were used to reveal the functions of miRNAs in rice under drought stress. These methods included target genes identification, differentially expressed miRNAs screening, enrichment analysis of DEGs, network constructions for miRNA-target and target-target proteins interaction. Results: (1) A total of 229 miRNAs with differential expression in rice under the drought stress, corresponding to 73 rice miRNAs families, were identified. (2) 1035 differentially expressed genes (DEGs) were identified, which included 357 up-regulated genes, 542 down-regulated genes and 136 up/down-regulated genes. (3) The network of regulatory relationships between 73 rice miRNAs families and 1035 DEGs was constructed. (4) 25 UP_KEYWORDS terms of DEGs, 125 GO terms and 7 pathways were obtained. (5) The protein-protein interaction network of 1035 DEGs was constructed. Conclusion: (1) MiRNA-regulated targets in rice might mainly involve in a series of basic biological processes and pathways under drought conditions. (2) MiRNAs in rice might play critical roles in Lignin degradation and ABA biosynthesis. (3) MiRNAs in rice might play an important role in drought signal perceiving and transduction.

scholarly journals Expression Profile and Prognostic Value of Wnt Signaling Pathway Molecules in Colorectal Cancer

Biomedicines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1331
Author(s):  
Yung-Fu Wu ◽  
Chih-Yang Wang ◽  
Wan-Chun Tang ◽  
Yu-Cheng Lee ◽  
Hoang Dang Khoa Ta ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Wnt Signaling ◽  
Signaling Pathway ◽  
Messenger Rna ◽  
Wnt Signaling Pathway ◽  
Interaction Network ◽  
Mrna Levels ◽  
Key Factor ◽  
Canonical Wnt ◽  
Upregulated Genes

Colorectal cancer (CRC) is a heterogeneous disease with changes in the genetic and epigenetic levels of various genes. The molecular assessment of CRC is gaining increasing attention, and furthermore, there is an increase in biomarker use for disease prognostication. Therefore, the identification of different gene biomarkers through messenger RNA (mRNA) abundance levels may be useful for capturing the complex effects of CRC. In this study, we demonstrate that the high mRNA levels of 10 upregulated genes (DPEP1, KRT80, FABP6, NKD2, FOXQ1, CEMIP, ETV4, TESC, FUT1, and GAS2) are observed in CRC cell lines and public CRC datasets. Moreover, we find that a high mRNA expression of DPEP1, NKD2, CEMIP, ETV4, TESC, or FUT1 is significantly correlated with a worse prognosis in CRC patients. Further investigation reveals that CTNNB1 is the key factor in the interaction of the canonical Wnt signaling pathway with 10 upregulated CRC-associated genes. In particular, we identify NKD2, FOXQ1, and CEMIP as three CTNNB1-regulated genes. Moreover, individual inhibition of the expression of three CTNNB1-regulated genes can cause the growth inhibition of CRC cells. This study reveals efficient biomarkers for the prognosis of CRC and provides a new molecular interaction network for CRC.

scholarly journals Genome-wide analysis of autophagy-related genes in Medicago truncatula highlights their roles in seed development and response to drought stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mingkang Yang ◽  
Liping Wang ◽  
Chumin Chen ◽  
Xu Guo ◽  
Chuanglie Lin ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Development ◽  
Medicago Truncatula ◽  
Interaction Network ◽  
Abiotic Stress Response ◽  
Biotic And Abiotic Stress ◽  
Comprehensive Overview ◽  
Network Analyses ◽  
Atg Genes ◽  
Gene Structures

AbstractAutophagy is a highly conserved process of degradation of cytoplasmic constituents in eukaryotes. It is involved in the growth and development of plants, as well as in biotic and abiotic stress response. Although autophagy-related (ATG) genes have been identified and characterized in many plant species, little is known about this process in Medicago truncatula. In this study, 39 ATGs were identified, and their gene structures and conserved domains were systematically characterized in M. truncatula. Many cis-elements, related to hormone and stress responsiveness, were identified in the promoters of MtATGs. Phylogenetic and interaction network analyses suggested that the function of MtATGs is evolutionarily conserved in Arabidopsis and M. truncatula. The expression of MtATGs, at varied levels, was detected in all examined tissues. In addition, most of the MtATGs were highly induced during seed development and drought stress, which indicates that autophagy plays an important role in seed development and responses to drought stress in M. truncatula. In conclusion, this study gives a comprehensive overview of MtATGs and provides important clues for further functional analysis of autophagy in M. truncatula.

scholarly journals Silencing of The Slzf-31 Gene Decreases The Salt Stress Tolerance And Drought Tolerance Of Tomato

2021 ◽  
Author(s):  
Tong Pei ◽  
Yufang Bao ◽  
Tairu Wu ◽  
Ziyu Wang ◽  
Yue Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Virus Induced Gene Silencing ◽  
Chlorophyll Fluorescence Parameters ◽  
Tomato Plants ◽  
Drought And Salt Stress ◽  
Stem Bending ◽  
Physiological Indexes ◽  
Mda Content ◽  
Aba Content

Abstract The SlZF-31 gene is a member of the tomato C2H2 transcription factor family. Previous studies have shown that SlZF-31 gene expression is upregulated under drought stress and salt stress, but the specific function of this gene in tomato plants in response to these two kinds of stress is still unclear. To further explore the function of the SlZF-31 gene in tomato under drought stress and salt stress, we employed the virus-induced gene silencing (VIGS) method to reduce the expression of the SlZF-31 gene in tomato. The results showed that TRV2-SlZF-31 plants had higher levels of wilt and stem bending than CK and CK-TRV2 plants under drought and salt stress. The ABA content of TRV2-SlZF-31 plants were lower than those of CK and CK-TRV2 plants. The analysis of physiological indexes showed that the SOD and POD activity and the PRO content of TRV2-SlZF-31 plants were lower than those of CK and CK-TRV2 plants, while the MDA content of TRV2-SLlZF-31 plants was higher than those of CK and CK-TRV2 plants. The accumulation of H2O2 and O2- in TRV2-SlZF-31 plants was greater than those in CK and CK-TRV2 plants. The values of the chlorophyll fluorescence parameters (ΦII and qL) of TRV2-SlZF-31 plants were significantly lower than those of CK and CK-TRV2 plants. These results showed that the silencing of the SlZF-31 gene reduces the drought resistance and salt tolerance of tomato.

scholarly journals STUDY ON SURVIVABILITY OF NEWLY HATCHED LARVAE OF TWO SPECIES OF PEPPERMINT SHRIMP FED WITH DIFFERENT COMBINATIONS AND DENSITIES OF LIVE FOODS

2019 ◽  
Vol 14 (1) ◽  
pp. 15
Author(s):  
Indra Pratama ◽  
Hatim Albasri
Keyword(s):  
Survival Rate ◽  
Larval Stage ◽  
Survival Rates ◽  
The Third ◽  
Randomized Design ◽  
Average Survival ◽  
Peppermint Shrimp ◽  
Trial Treatment ◽  
Newly Hatched Larvae ◽  
Average Survival Rate

This study was aimed to evaluate different types and combinations of live foods in relation to the survivability of newly hatched Lysmata vittata and Lysmata intermedia larvae. The experiment consisted of three trials (different species, combinations, and densities of live foods) arranged in a completely randomized design. The first and second trials were subjected to L. vittata with three treatments for each trial (1A, 1B, 1C for trial-1; 1D, 1E, 1F for trial-2). The third trial consisted of two treatments (2A and 2B) tested on L. intermedia. Each treatment had three replicates. The results showed that the survival rates were low in all treatments. However, each treatment showed a significant effect (P<0.05) on the average survival rate of L. vittata and L. intermedia larvae. In the first trial, treatment 1C was the only one that had survived larvae after day 35 with 4.44% of final average survival rate (FASR). Four of the larvae reached the post-larval stage. In the second trial, treatment 1F showed a better condition than the other treatments with 5.56% FASR. Nevertheless, no larvae in the second trial had transformed to post-larval stage before the experiment ended at day 46. In the third trial, no larvae survived to reach the post-larval stage. In spite of this, treatment 2B had better daily average survival rate (DASR) than treatment 2A. This research concludes that the use of copepods as live food at an early larval stage and Artemia at a later stage is relatively more effective to improve the survival rate of peppermint shrimp larvae.

scholarly journals Mortality of Atta sexdens rubropilosa (Hymenoptera: Formicidae) workers in contact with colony waste from different plant sources

2009 ◽  
Vol 100 (1) ◽  
pp. 99-103 ◽  
Author(s):  
F.G. Lacerda ◽  
T.M.C. Della Lucia ◽  
O.L. Pereira ◽  
L.A. Peternelli ◽  
M.R. Tótola
Keyword(s):  
Survival Rate ◽  
Trichoderma Viride ◽  
Pathogenic Microorganisms ◽  
Atta Sexdens ◽  
Atta Sexdens Rubropilosa ◽  
Average Survival ◽  
Ligustrum Japonicum ◽  
Acalypha Wilkesiana ◽  
Plant Sources ◽  
Average Survival Rate

AbstractThe objective of this work was to study the effect of colony waste on the mortality of workers of Atta sexdens rubropilosa Forel colonies fed with different plant substrates. Eight nests were used; two colonies each were fed with Acalypha wilkesiana Müller.Arg, Ligustrum japonicum Thunb, Eucalyptus urophylla S.T. Blake or a mixture of the three substrates in equal proportions. Irrespective of diet, being kept with waste led to higher mortality. However, workers that were kept in contact with waste produced by colonies fed Acalypha had higher average survival rate and later death when compared with workers from the other treatments. Workers from the Eucalyptus-fed colonies had the lowest survival rate and 50% of them died within four days of exposure to Eucalyptus waste. Trichoderma viride Pers. ex Gray, a fungus garden antagonist, and the entomopathogen Aspergillus flavus Link. ex Gray were present in the colonies supplied with all plants. The largest fungus diversity was verified in the waste of colonies fed Acalypha and the mixture of Acalypha, Ligustrum and Eucalyptus. Therefore, antibiotic properties of Acalypha did not reduce contaminant diversity but apparently minimized effects of pathogenic microorganisms present in the waste such as Asp. flavus. This may explain the highest survival rate of workers in this treatment.

Physiological indexes of six plant species from the tibetan plateau under drought stress

2014 ◽  
Vol 34 (13) ◽  
Author(s):  
潘昕 PAN Xin ◽  
邱权 QIU Quan ◽  
李吉跃 LI Jiyue ◽  
王军辉 WANG Junhui ◽  
何茜 HE Qian ◽  
...  
Keyword(s):  
Drought Stress ◽  
Tibetan Plateau ◽  
Plant Species ◽  
The Tibetan Plateau ◽  
Physiological Indexes

DEVELOPMENT OF AN INTRODUCTION PROTOCOL FOR STRAWBERRY PLANTS INTO IN VITRO CULTURE

2020 ◽  
Vol 5 (86) ◽  
pp. 45-50
Author(s):  
O.V. Mazneva ◽  
◽  
L.V. Tashmatova ◽  
T.M. Khromova ◽  
V.V. Shakhov ◽  
...  
Keyword(s):  
Survival Rate ◽  
In Vitro Culture ◽  
Plant Material ◽  
Survival Rates ◽  
Strawberry Plant ◽  
Active Growth ◽  
High Viability ◽  
Average Survival ◽  
Average Survival Rate

The research was conducted in order to develop an effective protocol for introducing strawberry plants into in vitro culture. The objects of the research were the most popular varieties of strawberries of domestic and foreign selection: Tsaritsa, Bereginya, Florence, Frida, Kimberly, etc. Mercurial preparations mertiolate at a concentration of 0.01% and sulema at a concentration of 0.1% were used as sterilizing agents. The isolation of explants was performed in several periods: the beginning of the growth was in February, active growth was in June, the decline of growth was in August. The studies have shown that the maximum aseptic cultures were obtained when processing strawberry plant material with mercurycontaining sulema preparation in the concentration of 0.1%. At the first stage of micropropagation, explants had a high viability during all periods of the isolation, the average survival rate for varieties was 74.8-80.7%. A significant influence of the genotype (varietal characteristics) on the survival rates of explants was noted. The number of explants suitable for cloning did not depend on the overall level of regeneration. Stabilization of the crop during winter introduction was much faster than in other periods. Using the winter term of the isolation of strawberry explants allowed to increase the yield of explants capable of further cloning, accelerate the stabilization of the culture in vitro and reduce the time for obtaining micro-plants suitable for planting in non-sterile conditions. On average, 75.2% of explants capable of further cloning for the varieties were obtained. As a result of the research, the conditions and methods for obtaining the largest number of viable sterile strawberry explants were worked out, which will be included into the process of reproduction in vitro and further research.

Effects of Drought Stress on Physiological Properties of Gazania rigens L.

2014 ◽  
Vol 955-959 ◽  
pp. 217-221 ◽  
Author(s):  
Yang Qing Wu ◽  
Xiao Yu Guo ◽  
Xiao Hui Zhou ◽  
Xiao Ping Lu
Keyword(s):  
Drought Stress ◽  
Drought Resistance ◽  
Significant Positive Correlation ◽  
Stress Treatment ◽  
Physiological Properties ◽  
Physiological Indexes ◽  
Mda Content ◽  
Relative Conductivity ◽  
Effects Of Drought ◽  
Physiological Indicator

Under artificial drought environment, membrane permeability, malondialdehyde (MDA) content and free proline (Pro) content of three cultivars of gazania were studied as well as the relation between the three physiological indexes and drought resistance. The results showed that: there existed significant positive correlation between relative conductivity and drought resistance in ‘Hongwen’; while ‘Xingbai’ and ‘XH’ showed the reverse result. The correlation between Pro content and drought stress treatment days in the three cultivars were significant positive. However, the correlation between MDA content and drought stress treatment days in the three cultivars were not the same. Therefore, Pro content can be chose as a physiological indicator to evaluate drought resistance in gazania.

Sign in / Sign up

Export Citation Format

Share Document