Pengaruh Pemberian Minuman Energi Terhadap Kadar Serum Kreatinin Rattus Norvegicus

The energy drink is a beverage that is increasingly being used by the community with the aim to improve work performance, vitality, and concentration. The energy drink is also suspected to affect kidney function. This study aims to determine whether there is influence of various brands of energy drinks (M1, M2, M3, M4) of the serum creatinine levels in male rats Rattus norvegicus.This is a study with randomized post test only control group design. The study used male rats Rattus norvegicus as many as 25 animals that were divided into 5 groups. The control group, the group P1 = M1 energy drinks, group P2 = M2 energy drinks, group P3 = M3 energy drinks, group P4 = M4 energy drinks. The energy drinks were given as many as 2.5 ml / 200 g weight / day for 1 month. Cardiac blood sampling performed to measure the levels of serum creatinine and then the results were analyzed using One-Way Anova to compare the levels of serum creatinine in each group of this study. Results of One-Way Anova test obtained by sig α = 0.000 which means there is significant difference between the treatment group and the control group. It can be concluded that the administration of energy drinks to the Rattus norvegicus would be altered the kidney function. It is caused by various substances contained in energy drinks such as taurine, sugar, vitamin B, caffeine, and other supplementary materials.

Download Full-text

Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

Jurnal Ilmiah Keperawatan STIKES Hang Tuah Surabaya ◽

10.30643/jiksht.v12i1.37 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Idola Perdana Sulistyoning Suharto

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Male Rats ◽

Control Group ◽

P Value ◽

Control Group Design ◽

White Rats ◽

Significant Difference ◽

Phaleria Macrocarpa ◽

Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound

Download Full-text

Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

Oceana Biomedicina Journal ◽

10.30649/obj.v3i2.47 ◽

2020 ◽

Vol 3 (2) ◽

pp. 38

Author(s):

Ayu Mayang A. P. ◽

Liliawanti Liliawanti ◽

Wachjudi Kurnia

Keyword(s):

Rattus Norvegicus ◽

White Male ◽

Brown Seaweed ◽

Negative Control ◽

Laboratory Method ◽

The Body ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

Significant Difference

Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (Sargassum duplicatum) which can increase hematocrit levels in the blood of white male wistar strain rat (Rattus norvegicus) suffered from anemia in induction NaNO2. This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K0) without treatment, the positive control (K1) be treated anemia given NaNO2 for 18 days without Sargassum duplicatum extract and treatment group (K2) treated with anemia given NaNO2 for 18 days without Sargassum duplicatum extract at a dose of 2,45gr / kgBB. On the 40th day, a blood sample was taken, then measured the hematocrit level. The results showed that the mean number of blood hematocrit levels in K2 was higher than K1, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p > 0,05 showed that there were no statistically significant differences between groups of experimental animals. From the results of this research, the conclusion is the administration of brown seaweed extract (Sargassum duplicatum) descriptively increased, but not statistically significant. Keywords : Anemia, hematocrit, Sargassum duplicatum, NaNO2

Download Full-text

UJI EFEKTIVITAS SENYAWA FLAVONOID DARI EKSTRAK ETANOL DAUN SAWILANGIT (Vernonia cinerea (L.) Less) SEBAGAI ANTIPIRETIK PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI VAKSIN DPT

PHARMACON ◽

10.35799/pha.9.2020.31367 ◽

2020 ◽

Vol 9 (4) ◽

pp. 572

Author(s):

Asriyani Samiun ◽

Edwin De Queljoe ◽

Irma Antasionasti

Keyword(s):

Rattus Norvegicus ◽

Ethanol Extract ◽

Male Rats ◽

Test Material ◽

Control Group ◽

White Rats ◽

Wistar Strain ◽

One Way Anova ◽

Vernonia Cinerea ◽

Dpt Vaccine

ABSTRACTSawilangit leaves (Vernonia Cinerea (L.) Less) is a plant that contains flavonoids as an antipyretic. The purpose of this study was to determine the effectiveness of flavonoid compounds from ethanol extract of sawilangit (Vernonia cinerea (L.) Less) leaves as an antipyretic in male white rats Wistar strain (Rattus norvegicus) induced by DPT vaccine. A total of 15 male rats were divided into 5 treatment groups, namely the negative control group (CMC 1%), the positive control group (paracetamol), and the ethanol extract group of sawilangit leaves with a dose of 200 mg, a dose of 400 mg, a dose of 800 mg. Each mouse was induced intramuscularly with a DPT vaccine at a dose of 0.3 ml. Temperature measurements were carried out before and after induction, then every 30 minutes after giving the test material from the 30th minute to the 180th minute. Data were analyzed statistically with One Way ANOVA and continued with LSD test to see significant differences between treatments. The results showed that the ethanol extract of sawilangit leaves had an antipyretic effect on male white rats Wistar strain..Key words: Antipyretics, Vernonia Cinerea, male white rats ABSTRAKDaun Sawilangit (Vernonia Cinerea (L.) Less) adalah salah satu tanaman yang mengandung zat Flavonoid sebagai antipiratik. Tujuan penelitian ini untuk mengetahui adanya efektivitas senyawa flavonoid dari ekstrak etanol daun sawilangit (Vernonia cinerea (L.) Less) sebagai antipiretik pada tikus putih jantan galur wistar (Rattus norvegicus) yang diinduksi vaksin DPT. Sebanyak 15 ekor tikus putih jantan dibagi 5 kelompok perlakuan yaitu kelompok kontrol negatif (CMC 1%), kelompok kontrol positif (parasetamol), dan kelompok ekstrak etanol daun sawilangit dosis 3,6 mg, dosis 7,2 mg, dosis 14,4 mg. setiap tikus diinduksi vaksin DPT dosis 0,3 ml secara intramuscular. Pengukuran suhu dilakuan sebelum dan sesudah induksi, kemudian setiap 30 menit setelah pemberian bahan uji dari menit ke-30 sampai menit ke-180. Data dianalisis secara statistika dengan One Way ANOVA dan dilanjutkan uji LSD untuk melihat beda nyata antar perlakuan. Hasil penelitian menunjukan bahwa Ekstrak etanol daun sawilangit mempunyai efek antipiretik terhadap tikus putih jantan galur wistar. Kata kunci: Antipiretik, Vernonia Cinerea, tikus putih jantan

Download Full-text

Effects of L-Carnitine on follicular reserve and Caspase-3 in transplanted mouse ovarian tissue

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v26i12.662 ◽

2019 ◽

Author(s):

Fatemeh Shahi Sadrabadi ◽

Kazem Parivar ◽

Hussein Imani ◽

Abdolhussein Shahverdi

Keyword(s):

Caspase 3 ◽

Sham Group ◽

Ischemia Reperfusion ◽

Ovarian Tissue ◽

Control Group ◽

Ovarian Tissue Transplantation ◽

Study Results ◽

Significant Difference ◽

Follicular Reserve ◽

One Way Anova

Introdution: One of the major challengs in ovarian tissue transplantation is overcomeing ischemia/ reperfusion injuries. During ischemia–reperfusion processes, oxygen free radicals constitute the most important component that induces damage of the grafted tissues. The aim of this study was to investigate the effect of L-Carnitine (LC) as an antioxidant on heterotopic transplantation of mouse ovarian tissue. Methods: In this laboratory experimental study, 5- week old female NMRI mice were divided into four groups: control, transplanted without administration (autograft), sham group (autograft+ saline) and LC group (autograft+ L- carnitine). Left ovarian tissues were transplanted into the Gluteal muscle for 3 weeks. After this time, ovarian tissues from all groups were removed and fixed in formalin for histological studies. Furthermore, rate of Caspase- 3 was assessed by immunohistochemistry test. Lipid peroxidation was assessed by measuring malondialdehyde (MDA). One-way ANOVA and Tudey test was used to analyze the data using the spss 16 software. Significance was defined as P≤0.0. Results: The study results indicated that total follicular count in transplantedwithout administration and sham groups was significantly lower than the control group (p<0.05), but there was no significant difference between the control and LC groups. In addition, the rate of caspase-3 was decreased in the LC group, but no significant difference existed between all groups (p<0.05). A significant reduction in the concentration of MDA was observed in the LC group than that in the other transplanted groups (p<0.05). Conclusion: In this study, LC could improve the ovarian reserve to some extent, but its effect was not significant.

Download Full-text

The Effect of Mahkota Dewa (Phaleria macrocarpa) Pulp Extract by Peroral Administration Toward The Percentage of Capacitation and Acrosome Reaction in Rat (Rattus norvegicus)

KnE Life Sciences ◽

10.18502/kls.v3i6.1199 ◽

2017 ◽

Vol 3 (6) ◽

pp. 694

Author(s):

Dhanang Estu Bagyo ◽

Budi Utomo ◽

Rudy Sukamto Setiabudi

Keyword(s):

Rattus Norvegicus ◽

Acrosome Reaction ◽

Male Rats ◽

Peroral Administration ◽

Male Rat ◽

Control Group ◽

Anova Analysis ◽

Phaleria Macrocarpa ◽

One Way Anova ◽

Average Body

This research was conducted to find outthe influence of mahkota dewa (Phaleria macrocarpa) pulp extract against the percentage of capacitation and acrosome reaction of male rat (Rattus norvegicus). The experimental animals used are 20 male rats with the average body weight of 200g and were divided into four different groups. P0 group was the control group and given only CMCNa 0.5%. P1, P2,and P3 was given mahkota dewa pulp extract with a dosage of 7.5 mg/200gr BW/ day, 15mg/200gr BW/day,and 30 mg/200gr BW/day respectively through peroral administration for 14 days. The research used Completely Randoized Design (CRD) and the data was then analyzed by one way ANOVA (Analysis of Variance) followed by Duncan test with the significance of 0.5. The results show that the mahkota dewa pulp extract shows significant impact on capacitation and acrosome reaction of rat. P2 group shows the highest percentage capacitation significance the a mean of 30.00 ± 4.24 and 5.60 ± 0.89 for acrosome reaction percentage. Keywords: Phaleria macrocarpa pulp extrtact; capasitation; acrosome reaction; sperm potention

Download Full-text

PENGARUH PEMBERIAN CUKA APEL (Malus sylvestris Mill) VARIETAS MANALAGI TERHADAP KADAR TRIGLISERIDA DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK DAN DIINDUKSI ALOKSAN

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v20i2.28 ◽

2019 ◽

Vol 20 (2) ◽

pp. 70-75

Author(s):

Yuliyanti Yuliyanti ◽

A. Sareh Arjono Tjandra ◽

Herin Setianingsih

Keyword(s):

Rattus Norvegicus ◽

High Fat Diet ◽

White Male ◽

Male Rats ◽

Control Group ◽

Standard Diet ◽

High Fat ◽

Malus Sylvestris ◽

Wistar Strain ◽

Significant Difference

Hypertriglyceridemia is one of the risk factors for cardiovascular disease and acute pancreatitis (Pejic and Lee, 2006; Brahm and Hegele, 2013; Afari et al., 2015). One way to treat it by using the apple vinegar (Malus sylvestris Mill) of manalagi varieties which contained high antioxidants and acetic acid as well as more durable than the fresh fruit. The study aimed to know the effect of the Apple vinegar of manalagi varieties (Malus sylvestris Mill) to blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. The study used the post test only control group design, with 24 rats samples which were divided into three groups. The negative control group (K0) only received standard diet for 24 days, the positive control group (K1) received a high-fat diet for 7 days and induced by alloxan with doses of 90 mg/kgBW of rats, and treatment group (K2) was given a high-fat diet for 7 days, induced by alloxan and received the apple vinegar (Malus sylvestris Mill) of manalagi varieties with a dose of 0,27 ml/100 g BW of rats for 14 days. The results by using one way ANOVA showed the significance value of the data p = 0,974 (p>0,05) so that there is no significant difference of the mean of blood triglycerides levels between K0, K1 and K2. This study concluded that apple vinegar (Malus sylvestris Mill) of manalagi varieties can not significantly lower the blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. Further study about apple vinegar of manalagi varieties is required to define its effects. Keywords—: High-Fat Diet, Alloxan, Triglyceride, Apple Vinegar (Malus sylvestris Mill) of Manalagi Varieties

Download Full-text

Pengaruh Pemberian Etanol Peroral terhadap Berat Testis Tikus Putih (Rattus norvegicus Strain Wistar)

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.555 ◽

2018 ◽

Vol 2 (1) ◽

pp. 7-11

Author(s):

Antonius Oktavian ◽

Elieser Elieser ◽

Agnes S. Rahayu ◽

Dais Iswanto

Keyword(s):

Rattus Norvegicus ◽

Reproductive Organ ◽

Male Reproduction ◽

Male Rats ◽

Control Group ◽

Wistar Strain ◽

Significant Difference ◽

The Difference ◽

Group 2 ◽

Level Of Significance

Alcohol (ethanol) has long been used as liquor. In some areas in Indonesia, the use of ethanol is an insaperable part of family rites and traditional festivities. However, due to its serious consequences, alcoholism becomes a chronic social problem. Ethanol effects the metabolism in body organs and tissues, including male reproductive organ. The objective of this study was to prove that ethanol comsumption may reduce the weight of testis. This study used Posttest Only Group Design with experimental animals of 30 male rats (Wistar strain Rattus norvegicus) that were subjected in five types of treatment, i.e,(1) feeding with aquades 2ml/day as control group (2) feeding with 10% 1 gr/Kg/day of ethanol, (3) feeding with 10% 3gr/Kg/day of ethanol, (4) feeding with 30% 1 gr/Kg/day of ethanol and (5) feeding with 30% 3 gr/Kg/day of ethanol. Treatment was given daily for 45 days. At the end of the study, on the day 46th, the rats was sacrified for examination. Sample of testis were taken for weight examination. Data were analysed using Anova and when the difference was found, the analysis was followed with Least significant Difference at the level of significance of 95%. The result of this study showed that ethanol reduced the weight of testis compared to control. The results of Anova test revealed that higher dosage and concentrations of ethanol resulted in significantly different decrease of the testis’s weight (p<0,05) compared to other dosage and other concetration. In conclusion, ethanol has effect in the reduction of the weight of the testis.Key words: ethanol, male reproduction, Rattus norvegicus, weight of testis.

Download Full-text

Investigating the effect of paricalcitol on serum FGF23 in vitamin D deficient rats

10.21203/rs.2.18457/v1 ◽

2019 ◽

Author(s):

Forough Saki ◽

Gholamhossein Ranjbar Omrani ◽

Farhad Koohpeyma

Keyword(s):

Vitamin D ◽

Laboratory Data ◽

Fractional Excretion ◽

Male Rats ◽

Control Group ◽

Deficient Diet ◽

Study Results ◽

Significant Difference ◽

Serum Biochemical ◽

Serum Pth

Abstract Background: We conducted this study to discover changes of serum FGF23 in non-uremic rat model of vitamin D deficiency without secondary hyperparathyroidism using paricalcitol. Methods: 30 adult male rats weighting 300±20 grams were enrolled. They were divided into three groups of 10 rats including Control, Vitamin D deficient(VDD), and Vitamin D deficient diet treated with paricalcitol(VDD+P). Serum biochemical were checked twice, at baseline and after the 22nd day of study. Results: There was no significant difference in baseline laboratory data between groups. At the end of the study, 1,25(OH)D3 was reduced in VDD (P = 0.019) and VDD+P (P < 0.001) with a more significant decline in VDD+P group. Serum level of FGF23 was reduced in VDD+P group compared to the control group (P = 0.011) and VDD group (P = 0.021). serum PTH in VDD group was higher than the control and VDD+P group (P = 0.036 and P = 0.038, respectively). Conclusion: The present study showed that paricalcitol could reduce FGF23 in vitamin D deficient rats without any changes in serum calcium, phosphorous and fractional excretion of phosphorous, which might be due to low PTH and 1,25(OH)2 D3.

Download Full-text

Pengaruh Pemberian Ekstrak Rumput Laut Merah (Kappaphycus alvarezii) terhadap Kadar LDL Pada Tikus Putih (Rattus Norvegicus) Jantan Galur Wistar Yang Diberi Diet Tinggi Lemak

Hang Tuah Medical journal ◽

10.30649/htmj.v15i2.47 ◽

2018 ◽

Vol 15 (2) ◽

pp. 112

Author(s):

MENTARI AMENDA SAPUTRI ◽

HERIN SETIANINGSIH

Keyword(s):

Rattus Norvegicus ◽

Kappaphycus Alvarezii ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Post Test ◽

One Way Anova ◽

Test Control

Penyakit kardiovaskular merupakan penyebab kematian nomor satu di dunia. Gaya hidup masyarakat terutama dalam mengkonsumsi diet yang tidak sehat dapat meningkatkan kadar LDL yang dapat menyebabkan penyakit kardiovaskular. Rumput laut merah (Kappaphycus alvarezii) yang banyak dibudidayakan di Indonesia mengandung flavonoid dan triterpenoid yang diduga dapat menurunkan kadar LDL. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak rumput laut merah (Kappaphycus alvarezii) terhadap kadar LDL pada tikus putih (Rattus norvegicus) jantan galur Wistar yang diberi diet tinggi lemak. Penelitian ini adalah penelitian eksperimental murni laboratorik dengan rancangan penelitian Post Test Control Group Design. Sampel yang digunakan adalah 24 ekor tikus putih (Rattus norvegicus) jantan galur Wistar yang dibagi ke dalam tiga kelompok: kelompok yang diberi diet standar selama 28 hari (K1), kelompok yang diberi diet tinggi lemak selama 28 hari (K2), dan kelompok yang diberi diet tinggi lemak selama 28 hari dan pada hari ke-15 sampai hari ke-28 diberi ekstrak rumput laut merah (Kappaphycus alvarezii) dengan dosis 140mg/200grBB/hari (K3). Hasil analisis statistik One Way Anova menunjukkan bahwa terdapat perbedaan kadar LDL yang signifikan antara ketiga kelompok pada penelitian ini (p<0,001). Kadar LDL pada K2 (=16,00±3,29) meningkat secara bermakna dibandingkan dengan K1 (=10,62±1,77). Sedangkan kadar LDL pada K3 (=6,88±2,42) menurun secara bermakna dibandingkan dengan K2. Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak rumput laut merah (Kappaphycus alvarezii) berpengaruh terhadap kadar LDL darah pada tikus putih (Rattus norvegicus) jantan galur Wistar yang diberi diet tinggi lemak.Kata kunci : diet tinggi lemak, LDL, Kappaphycus alvarezii

Download Full-text

The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽

10.3390/ani11061569 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1569

Author(s):

Tomislav Šušnjar ◽

Ivana Kuzmić Prusac ◽

Ivan Švagelj ◽

Anđela Jurišić ◽

Tomislav Šušnjar ◽

...

Keyword(s):

Sperm Count ◽

Semen Analysis ◽

Study Groups ◽

Testicular Biopsy ◽

Male Rats ◽

Lower Percentage ◽

Control Group ◽

Sprague Dawley ◽

Left Testis ◽

Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Download Full-text