scholarly journals Evaluating Positive and Negative Effects of Seven Biogenic Metal-based Nanoparticles on Seed Germination and Seedling of Nano-primed Wheat and Flax Seeds

Author(s):  
Maryam Bayat ◽  
Meisam Zargar

Abstract Seed germination is the first and the most susceptible stage in plant’s growing phases, so could be considered as an index to evaluate the effect of newly developed materials like nanoparticles (NPs), providing useful information for researchers. In our experiments, germination tests have been carried out in Petri plates, containing wet filter paper and nano-primed seeds. We had biosynthesized seven nanoparticles in our previous researches, including calcinated and non-calcinated zinc oxide, zinc, magnesium oxide, silver, copper and iron nanoparticles. The effect of these biogenic nanoparticles and their counterpart metallic salts including zinc acetate, magnesium sulphate, silver nitrate, copper sulphate and iron (III) chloride was studied on two popularly grown plants, wheat and flax, in laboratory condition to obtain preliminary information for future field experiments. Germination percentage, shoot length, root length, seedlings length, root-shoot ratio, seedling vigor index (SVI), shoot length stress tolerance index (SLSI) and root length stress tolerance index (RLSI) were calculated at 2nd and 7th days of the experiment. According to the results, the response of the plants to metal containing nanoparticles and metal salts mainly depend on type of the metal, plant species, concentration of the NP suspension or salt solution, condition of the exposure and the stage of growth.

Author(s):  
Shler Hassan Mahmood, Jalal Hama Salih Ismael

An experiment was conducted to study the antifungal activity of different concentrations aqueous extracts from three local plants, Hypericum triquetrifolium, Salix alba, Salvia rosmarinus and also different concentrations of fungicide (Captan) and Salicylic acid were evaluated for their efficacy as seed dressing on Catalpa and Albizia that seeds were soaked separately for 30 mints in various concentrations of aqueous extract and fungicide. Forest seeds were grown in pots which were inoculated by Sclerotinia sclerotiorum and Fusarium oxysporum that one petri plate/ pot in 2018 in a greenhouse at the University of Sulaimani–College of Agricultural Sciences Engineering. The results showed that seed germination percentage, shoot length, root length and number of leaves was significantly (p≤0.05) increased with both the treatments. Captan (0.5%) was more effective in comparison with Captan (0.3%) and seed germination, shoot length, root length and number of leaves was gradually increased with increase of the doses of fungicides. It can be suggested that fungicide Captan have antifungal effect and can be used to treat seed against seed borne pathogens of forest, increase seed germination and shoot length, root length and number of leaves.


2016 ◽  
Vol 8 (1) ◽  
pp. 84-87
Author(s):  
Bhim Jyoti ◽  
S. S. Gaurav ◽  
Usha Pant

Seeds of tomato cv. Keabi were primed with of three important growth regulators viz Gibberellic acid (GA3), Napthlene acetic acid (NAA) and potassium nitrate (KNO3). Four concentration of each, Gibberellic acid and Napthlene acitic acid (25ppm, 50ppm, 75ppm.100ppm) and two concentration of KNO3 (1% and 2%) were compared with the distilled water as control. For each treatment seeds were soaked in growth regulator for 24 hrs before put for the germination test. Fifty seeds in four replications were germinated on top of paper at 200C in seed germinator for 14 days. Normally germinated seedlings were counted which gave an estimation of germination percentage. Data was recorded on the germination percentage, shoot length, seed vigour index. Most of the treatments had significant positive effect on all the quality parameters. NAA had showed adverse effect on the root length while other growth regulators were found to be significant role to improve the root length. Maximum seed germination (74%) was observed at 50 and 75ppm GA3. Highest shoot length (4.83cm) was found at 25ppm of GA3 whereas enhancement of root length occurred with the priming of 1% KNO3 (3.52cm ). Seed vigour-I, on the basis of seedling length was observed higher at 25ppm. GA3 (720) and seed vigour –II on the basis of seedling dry weight was also observed maximum priming with 100ppm GA3 (1460). From this study it was suggested that GA3 priming has important growth regulator to enhance the seed germination as well as seed vigour. Before sowing seed should be priming with GA3 for obtaining high % germination and vigorous seedling that survive under adverse condition it also increase the uniformity of field plant stand.


2019 ◽  
Vol 11 (2) ◽  
pp. 321-326
Author(s):  
Sanchita Bhattacharya ◽  
Sourav Debnath ◽  
Sanjit Debnath ◽  
Ajay Krishna Saha

Growth attributes of Vigna mungo and Vigna radiata crop influenced by level and type of fertilizers. The present study was carried out to evaluate and compare the effects of organic (vermicompost) and inorganic (urea) fertilizers on the germination percentage and seedling growth of V. radiata (Green gram) and V. mungo (Black gram). Fresh weight and dry weight of tested plant samples at 10th days of growth stage were also determined. Vermicompost was used as organic fertilizer and urea as inorganic fertilizer. Experimental results showed that vermicompost and urea both has positive effect on seedling growth parameters of V. mungo and V. radiata [average root length (6.1cm and 6.7cm)] , shoot length (6.5cm and 8.3cm), leaf area (312.2 sq.cm and 334.1 sq.cm] as compared to control set [average root length (4.4cm and 4.3cm)] , shoot length (6cm and 5.9cm), leaf area (282.7 sq.cm and 305.5 sq.cm). But urea exerts negative effect on seed germination percentage in V. mungo and V. radiata (58% and 50%) as compared to control (77%). Vermicompost exhibited better result in above parameters in comparison to urea. V. mungo showed increased value in comparison considering V. radiata in case of organic, inorganic fertilizer treated as well as control sets. As composition of locally available fertilizers is unknown, the application of these fertilizers for improving germination percentage and growth parameters of tested crop need to be evaluated. Present work may provide the suggestive approach for usage of these tested fertilizers in field level trial.


2021 ◽  
Vol 43 ◽  
Author(s):  
Nancy Araceli Godínez-Garrido ◽  
Juan Gabriel Ramírez-Pimentel ◽  
Jorge Covarrubias-Prieto ◽  
Francisco Cervantes-Ortiz ◽  
Artemio Pérez-López ◽  
...  

Abstract: Chitosan is a biopolymer obtained from deacetylation of chitin; it has multiple applications in agriculture as an antifungal, soil conditioner, inducer of defense mechanisms, fruits postharvest coating, leaves and seeds, among others. The objective in this research was to evaluate the effect of chitosan coatings mixed with fungicide (dithiocarbamate) on the germination and germination speed of bean and maize seeds in storage and to determine the retention capacity of the fungicide in the coated seeds under different times of imbibition. Two coating treatments at concentrations of 0.1 and 0.5% chitosan in water, two coatings treatments at 0.1 and 0.5% chitosan supplemented with 0.5% fungicide and a coating without chitosan using only 0.5% fungicide in water were used in bean and maize seed; and as control seeds imbibed in distilled water were used; after treatments, germination percentage and germination speed were determined, also fungicide release were determined at 0, 1, 2 and 6 h of imbibition, and the effect of storage time on germination and germination speed was determined at 30, 60, 90, 120, 150 and 180 days of storage at 4 °C and 45% relative humidity. The fungicide release effect was determined by inhibiting Fusarium oxysporum conidia germination. There were no negative effects of coatings on seed germination after storage. The treatment that provided both greater retention of the fungicidal agent and released it gradually, was 0.5% chitosan mixed with fungicide concentration. Chitosan coating seeds mixed with fungicide do not cause negative changes in seed germination or germination rate.


2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.


Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Ahmad Dadashpour

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.


2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


2017 ◽  
Vol 3 (1) ◽  
pp. 140-144
Author(s):  
A Rokib ◽  
MS Monjil

Effectiveness of six fungicides viz., Provax-200 (Carboxin+Thiram), Bavistin DF (Carbendazim), Dithane M-45 (Mancozeb), Secure (Mancozeb+Fenamidone), Antracol (propineb) and Daconil (Chlorothalonil) were evaluated to improve seed germination and seedling vigour of lentil variety BINA Masur-3. The experiment was conducted in the Green House of Seed Pathology Centre, Bangladesh Agricultural University, Mymensingh. Positive effect of seed treatment with fungicides on seed germination and seedling vigour of lentil was recorded. At 20 DAS, increased seed germination over control was observed in treated seeds with Deconil (10.40%) and Antracol (10.00%) followed by Dithane M-45 (7.20%). Seeds treated with Deconil and Dithane M-45 produced seedlings with higher shoot length, root length and seedling vigour. At 20 DAS, higher percent increased vigour index over control was found in Dithane M-45 (24.64%) and Deconil (22.44%), respectively.Asian J. Med. Biol. Res. March 2017, 3(1): 140-144


Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.


Author(s):  
Sabita Ghimire ◽  
Hariom Yadav ◽  
Prabesh Acharya ◽  
Raju Kharel

This study aimed to determine the effect of seed priming (on-farm, hydro, halo, osmo, vitamin c priming, and hardening) on the germination and seedling growth of spring rice varieties: hardinath-3, hardinath-1, and chaite-5 at ambient room temperature. A factorial completely randomized design (CRD) was carried out with two factors: methods of priming and varieties at agriculture knowledge center (AKC), kapilbastu during march 2020. There were, in total, 21 treatment combinations and four replications. Parameters observed were germination percentage, speed of germination, vigor index, root length, shoot length, fresh weight, and dry weight of rice seedlings. Hardinath-3 had superior performance in terms of germination percentage (95.35 %), vigor index (183.86), root length (11.51 cm) and shoot length (7.39 cm) compared to hardinath-1 and chaite-5; the speed of germination, however, was greater in hardinath-1. Seed hardening - alternate soaking (tap water for 24 hours) and drying - induced higher germination percentage (94.91 %), speed of germination (96.00), and vigor index (197.43) than did other priming methods; germination percentage of vitamin c primed seeds (94.75 %), nevertheless, were at statistical par with hardened seeds. Hardened hardinath-3 seeds were better in regards to germination percentage (97.50 %) and root length (12.92 cm), while the speed of germination (97.50) and vigor index (210.50) was greater in hardened hardinath-1. Vitamin c primed seeds of hardinath-3 and hardinath-1 were finer with dry weight, shoot length, and vigor index. In conclusion, farmers' are suggested to hardened seeds of hardinath-3 before sowing to promote uniform germination and growth.


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