scholarly journals Heavy Metal Bioremediation by Novel Microbial Strains Proteus Mirabilis and Bordetella Avium

2021 ◽  
Author(s):  
Rohini Ganorkar ◽  
Niti B. Jadeja ◽  
Arti Shanware
Keyword(s):  
Heavy Metal ◽  
Proteus Mirabilis ◽  
Azo Dye ◽  
Dye Degradation ◽  
Soil Samples ◽  
Microbiological Analysis ◽  
Bordetella Avium ◽  
Icp Ms ◽  
Antibiotic Susceptibility Test ◽  
Xrf Analyses

Abstract The entitled study focuses on exploring the microbial diversity and its applicability in the remediation of metal contaminated soil using microbes which is a reliable, and cost effective technique. Present investigation studied microbiota present in tungsten enriched soil of kuhi-Agargaon-Khobna region using culturable approach confirmed by ICP-MS and SEM-EDS analysis. Furthur applicability in Bioremediation and Azo Dye degradation was studied. XRF analyses of soil samples were performed followed by traditional microbiological analysis for isolation of tungsten tolerant bacteria’s. Tungsten accumulation was confirmed using ICP-MS and SEM-EDS techniques. Different metals and azo dye tolerance studies were conducted. Antibiotic susceptibility test revealed the antibiotic resistance profile of these microbes. XRF analyses of soil samples from these regions measured about 300 ppm tungsten/kg of soil at the Agargaon region and 200 ppm tungsten/kg of soil at the Khobna region. The traditional microbiological analysis resulted in two bacterial isolates which were identified for tolerance to tungsten named as Proteus mirabilis strain RS2K and Bordetella avium strain RS3K through 16S rDNA gene sequencing and phylogenetic analysis. These microbes were found to accumulate tungsten intracellularly as confirmed through ICP-MS and SEM-EDS analyses. In addition to (sodium) tungsten, the microbes were tolerant to tungstic acid, ammonium metaparatungstate, mercuric chloride, cobalt chloride and azo dyes .Microbes exhibited well-equipped cellular mechanisms for metal tolerance to survive in heavy metal-laden ecology. The novel strains obtained through a culturable approach in this study contain substantial potential in bioleaching of heavy metals and green mining.

scholarly journals Measuring heavy metal content in bone using portable X-ray fluorescence

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Nicole C. Little ◽  
Victoria Florey ◽  
Irma Molina ◽  
Douglas W. Owsley ◽  
Robert J. Speakman
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Inductively Coupled Plasma ◽  
Analytical Techniques ◽  
Human Populations ◽  
Portable Xrf ◽  
X Ray ◽  
Icp Ms ◽  
Time Required ◽  
Xrf Analyses

The ability of inorganic-based analytical chemistry techniques to quantify trace amounts of heavy metals in skeletal remains has been integral for understanding health and social status in human populations. Low detection limits and the sensitivity of inductively coupled plasma- mass spectrometry (ICP-MS) and other techniques to most elements on the periodic table are ideally suited for the quantification of lead (Pb) and other heavy metals in bone. However, the time required for sample preparation and analysis, expense, destructive analytical process, and availability of instrumentation often limit researchers’ ability to utilise these techniques for archaeological applications. This paper explores the use of portable X-ray fluorescence (XRF) instrumentation for heavy metal analysis of bone as an alternative to more traditional analytical techniques. XRF has been shown to be an extremely useful tool for archaeologists seeking to conduct quantitative analyses of cultural materials such as obsidian and metals. However, little research has been undertaken to assess the usefulness of portable XRF for measuring heavy metals found in low concentrations in archaeological bone. This paper compares data derived from ICP-MS and portable XRF analyses of bone. Results demonstrate that XRF analyses of bone are problematic due to diagenesis and variability of Pb content in bone.

scholarly journals Investigation of an acute neurological outbreak in Eluru, India, 2020

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259192
Author(s):  
Mahesh Kumar Mummadi ◽  
Raghavendra Pandurangi ◽  
J. J. Babu Geddam ◽  
Sukesh Narayan Sinha ◽  
Ananthan Rajendran ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Water Samples ◽  
Biological Samples ◽  
Food Samples ◽  
Loss Of Consciousness ◽  
Microbiological Analysis ◽  
Organophosphate Pesticide ◽  
Household Level ◽  
Icp Ms ◽  
Study Participants

On 4th December 2020, a sudden outbreak, with neurological symptoms like seizures, loss of consciousness etc., was reported in a town from south India. By 3rd day about 400 people were involved. A multi disciplinary team from our institute visited the site to investigate the outbreak. Based on the case history and clinical examination of the patients, the team suspected a probable diagnosis of an acute pesticide, heavy metal and/or mycotoxin exposure for which, biological samples (blood, urine) were collected from those who reported the symptoms as well as from a few who did not report symptoms (controls). To identify the source, water and food samples were collected. The samples were subjected to ICP-MS for heavy metal analysis, LC-MS/MS for pesticide analysis, microbiological analysis and ELISA-Kit method for aflatoxins if any. Clinical and dietary details were collected from a total of 112 participants, of which, 103 cases (77 active cases at Hospital and 26 recovered cases from community) and 9 were controls. A total of 109 biological samples, 36 water samples and food samples were collected. The mean age of the study participants was 29.2 years. Among cases, Seizures were seen in 84%, loss of consciousness in 66%, mental confusion in 35%, pinpoint pupil in 11%. Triazophos (organophosphate) pesticide was present in 74% of Blood samples and its metabolites were present in 98% of the urine samples collected from the cases. All the ten heavy metals investigated including lead, mercury and nickel were found to be within permissible limits except for a few samples. No presence of mycotoxins was observed in Food samples. Water samples which included Head pump and reservoir were free from pesticides; however, all water samples from households of cases had triazophos pesticide with a mean concentration of 1.00 ug/L. Thus, it was concluded that, the probable cause of outbreak was Triazophos (Organophosphate) pesticide contamination in water at the Household level. Regular surveillance for the presence of residual pesticides in soil, water and food with heightened vigour is recommended to prevent future outbreaks.

scholarly journals Development of a Tellurium Speciation Study Using IC-ICP-MS on Soil Samples Taken from an Area Associated with the Storage, Processing, and Recovery of Electrowaste

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2651
Author(s):  
Magdalena Jabłońska-Czapla ◽  
Katarzyna Grygoyć
Keyword(s):  
Inductively Coupled Plasma ◽  
Total Concentration ◽  
Soil Samples ◽  
Chemical Extraction ◽  
Soil Extraction ◽  
Inductively Coupled ◽  
Sequential Chemical Extraction ◽  
Icp Ms ◽  
Speciation Study ◽  
Plasma Mass Spectrometry

The optimization and validation of a methodology for determining and extracting inorganic ionic Te(VI) and Te(IV) forms in easily-leached fractions of soil by Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry (IC-ICP-MS) were studied. In this paper, the total concentration of Te, pH, and red-ox potential were determined. Ions were successfully separated in 4 min on a Hamilton PRPX100 column with 0.002 mg/kg and 0.004 mg/kg limits of detection for Te(VI) and Te(IV), respectively. Soil samples were collected from areas subjected to the influence of an electrowaste processing and sorting plant. Sequential chemical extraction of soils showed that tellurium was bound mainly with sulphides, organic matter, and silicates. Optimization of soil extraction allowed 20% average extraction efficiency to be obtained, using 100 mM citric acid as the extractant. In the tested soil samples, both tellurium species were present. In most cases, the soils contained a reduced Te form, or the concentrations of both species were similar.

scholarly journals Chemical Properties of Heavy Metal-Contaminated Soils from a Korean Military Shooting Range: Evaluation of Pb Sources Using Pb Isotope Ratios

2021 ◽  
Vol 11 (15) ◽  
pp. 7099
Author(s):  
Inkyeong Moon ◽  
Honghyun Kim ◽  
Sangjo Jeong ◽  
Hyungjin Choi ◽  
Jungtae Park ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Management Strategies ◽  
Chemical Properties ◽  
Heavy Metal Concentration ◽  
Soil Samples ◽  
Shooting Range ◽  
Geochemical Properties ◽  
Mineral Phases

In this study, the geochemical properties of heavy metal-contaminated soils from a Korean military shooting range were analyzed. The chemical behavior of heavy metals was determined by analyzing the soil pH, heavy metal concentration, mineral composition, and Pb isotopes. In total, 24 soil samples were collected from a Korean military shooting range. The soil samples consist of quartz, albite, microcline, muscovite/illite, kaolinite, chlorite, and calcite. Lead minerals, such as hydrocerussite and anglesite, which are indicative of a transformation into secondary mineral phases, were not observed. All soils were strongly contaminated with Pb with minor concentrations of Cu, Ni, Cd, and Zn. Arsenic was rarely detected. The obtained results are indicated that the soils from the shooting range are contaminated with heavy metals and have evidences of different degree of anthropogenic Pb sources. This study is crucial for the evaluation of heavy metal-contaminated soils in shooting ranges and their environmental effect as well as for the establishment of management strategies for the mitigation of environmental risks.

Degradation of an Azo Dye by Fenton Type Processes Assisted with UV Irradiation

2007 ◽  
Vol 5 (1) ◽  
Author(s):  
Natalija Koprivanac ◽  
Dinko Vujevic
Keyword(s):  
Uv Irradiation ◽  
Azo Dye ◽  
Dye Degradation ◽  
Degradation Products ◽  
Batch Reactor ◽  
Process Efficiency ◽  
Molar Ratio ◽  
Synthetic Dyes ◽  
Ambient Conditions ◽  
Treatment Methods

Organic synthetic dyes are widely produced and used today. Significant losses of organic and inorganic content occurs during the manufacturing and application of dyes and its discharge in the effluent presents a threat to the eco-systems due to general toxicity and resistance to destruction by biological treatment methods. Particularly azo dyes are of special environmental concern due to their degradation products such as aromatic amines, which are considered highly carcinogenic. So, dyes have to be removed from coloured wastewater before discharge. However, traditional treatment methods (adsorption, coagulation/flocculation) mainly transfer the contaminants from wastewater to secondary waste. Therefore, advanced oxidation processes seem to be sustainable and clean technology to decolorize and minimize organic dyes content from wastewater. In this paper, degradation of an azo dye C.I. Direct Orange 39 (DO39) using Fenton type processes (Fe2+/H2O2, Fe3+/H2O2and Fe0/H2O2) has been performed. The molar ratio of Fenton’s type reagents has been varied in the range of 1 : 5 up to 1 : 50 at 0.5 and 1.0 mM concentrations of iron salts and iron powder. Experiments have been conducted for two hours in a batch reactor with magnetic stirring, ambient conditions and pH 3. The process efficiency and formation of degradation by-products have been determined on the basis of results obtained by UV/VIS spectrophotometric, total organic carbon (TOC) and high performance liquid chromatography (HPLC) analyses. The optimal Fenton and Fenton ``like" processes parameters have been applied in the photo reactor, too. It has been observed that simultaneous utilization of UV irradiation with Fenton's and Fenton ``like" reagents increases the degradation of DO39 dye. Degradation of the dye in dilute aqueous solution follows pseudo-first order kinetics. The maximal decolourization of 20 mg L-1 DO39 in water of 93.2% and TOC degradation of 76.9% were obtained using Fe3+/H2O2= 1 : 5 molar ratio. The results indicate that the treatment of DO39 dye wastewater with UV/Fe3 +/H2O2 system was found to be the most efficient.

scholarly journals Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Grab Sampling ◽  
Antibiotic Resistance Pattern ◽  
Antibiotic Susceptibility Test ◽  
Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 

scholarly journals Azo dye degradation by Phanerochaete chrysosporium in the medium enriched with nitrogen in the presence of primary co-substrate

2013 ◽  
Vol 56 (5) ◽  
pp. 867-874 ◽  
Author(s):  
Marcus Vinicius Freire Andrade ◽  
Karla Mayara Lima da Silva ◽  
João Paulo da Silva Siqueira ◽  
Carlos Ronald Pessoa Wanderley ◽  
Rinaldo dos Santos Araújo ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Azo Dye ◽  
Dye Degradation

Antimony speciation in soil samples along two Austrian motorways by HPLC-ID-ICP-MS

2005 ◽  
Vol 7 (12) ◽  
pp. 1200 ◽  
Author(s):  
Sameer Amereih ◽  
Thomas Meisel ◽  
Robert Scholger ◽  
Wolfhard Wegscheider
Keyword(s):  
Soil Samples ◽  
Icp Ms ◽  
Antimony Speciation

scholarly journals Spatial Connections between Microplastics and Heavy Metal Pollution within Floodplain Soils

2022 ◽  
Vol 12 (2) ◽  
pp. 595
Author(s):  
Collin J. Weber ◽  
Jens Hahn ◽  
Christian Opp
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Wide Distribution ◽  
Soil Material ◽  
Statistical Correlations ◽  
Floodplain Soils ◽  
Icp Ms ◽  
Metal Contents ◽  
Sediment Layers ◽  
Deposition Conditions

Soils contain an increasing number of different pollutants, which are often released into the environment by human activity. Among the “new” potential pollutants are plastics and microplastics. “Recognized” pollutants such as heavy metals, of geogenic and anthropogenic origin, now meet purely anthropogenic contaminants such as plastic particles. Those can meet especially in floodplain landscapes and floodplain soils, because of their function as a temporary sink for sediments, nutrients, and pollutants. Based on a geospatial sampling approach, we analyzed the soil properties and heavy metal contents (ICP-MS) in soil material and macroplastic particles, and calculated total plastic concentrations (Ptot) from preliminary studies. Those data were used to investigate spatial connections between both groups of pollutants. Our results from the example of the Lahn river catchment show a low-to-moderate contamination of the floodplain soils with heavy metals and a wide distribution of plastic contents up to a depth of two meters. Furthermore, we were able to document heavy metal contents in macroplastic particles. Spatial and statistical correlations between both pollutants were found. Those correlations are mainly expressed by a comparable variability in concentrations across the catchment and in a common accumulation in topsoil and upper soil or sediment layers (0–50 cm). The results indicate comparable deposition conditions of both pollutants in the floodplain system.

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