scholarly journals First Report of Canine Bufavirus and Its Whole Genome Sequence in India

2021 ◽  
Author(s):  
Vishweshwar Kumar Ganji ◽  
Bhagyalakshmi Buddala ◽  
Narasimha Reddy Yella ◽  
Kalyani Putty
Keyword(s):  
Respiratory Symptoms ◽  
Close Relation ◽  
The Other ◽  
Whole Genome Sequence ◽  
Epidemiological Surveillance ◽  
Sea Otter ◽  
First Report ◽  
Faecal Samples ◽  
Asian Lineage ◽  
Telangana State

Abstract Canine bufavirus (CaBuV), a novel protoparvovirus of dogs was reported only in Italy and China, till date. It was detected from dogs with enteric and respiratory symptoms and is distantly related to the human bufavirus. To explore the enteric prevalence of CaBuV in India, 186 diarrheic faecal samples were collected in Telangana State between 2019 and 2020. Among the samples, 4.3% (8/186) were positive for CaBuV by PCR. Co-infection with canine parvovirus CPV-2 was seen in 75% (6/8) of CaBuV positive samples. The near complete genome (4292 bp) of CaBuV was amplified and reconstructed for one isolate 407/PVNRTVU/2020. Sequence alignment indicated 93.42–98.81% homology with the other available CaBuV sequences; 70.88-73.39%, and 54.4-54.8% identity with human bufavirus, and CPV-2, respectively. Phylogenetic analysis showed that CaBuV 407/PVNRTVU/2020 was most closely related to CaBuV Chinese strains, together separated as Asian lineage. All Bufaviruses clustered together in one clade; however, the bat and sea otter parvoviruses also showed close relation with Bufaviruses. This first report of prevalence of CaBuV in India provides a good reference, emphasizes the need for further epidemiological surveillance of CaBuV in India and its role in canine enteritis.

The Other Entrepreneurs - Migrant Economies as Spaces for Social Innovation?

2019 ◽  
Vol 16 (2) ◽  
pp. 265-271
Author(s):  
Claudia Lintner
Keyword(s):  
Social Innovation ◽  
Close Relation ◽  
The Other ◽  
Structural Level ◽  
Structured Interviews ◽  
Economic Activities ◽  
Migrant Entrepreneurship ◽  
False Dichotomy ◽  
Migrant Entrepreneurs ◽  
The One

This article analyses the relationship between migrant entrepreneurship, marginalisation and social innovation. It does so, by looking how their ‘otherness’ is used on the one hand to reproduce their marginalised situation in society and on the other to develop new living and working arrangements promoting social innovation in society. The paper is based on a qualitative study, which was carried out from March 2014- 2016. In this period, twenty semi-structured interviews were conducted with migrant entrepreneurs and experts. As the results show, migrant entrepreneurs are characterised by a false dichotomy of “native weakness” in economic self-organisation against the “classical strength” of majority entrepreneurs. It is shown that new possibilities of acting in the context of migrant entrepreneurship are mostly organised in close relation to the lifeworlds and specific needs deriving from this sphere. Social innovation processes initiated by migrant entrepreneurs through their economic activities thus develop on a micro level and are hence less apparent. Supportive networks are missing on a structural level, so it becomes difficult for single innovative initiatives to be long-lasting.

Human density, economic level and frequency of canine helminths in Buenos Aires

2021 ◽  
Vol 95 ◽  
Author(s):  
D. Rubel ◽  
N. Flaibani
Keyword(s):  
Linear Models ◽  
Buenos Aires ◽  
Toxocara Canis ◽  
The Other ◽  
Green Spaces ◽  
Cross Sectional ◽  
Independent Variables ◽  
Economic Level ◽  
Faecal Samples ◽  
Human Density

Abstract The aim of this study was to explore through cross-sectional study the variation in the prevalence of parasitic helminths in canine faeces collected from green spaces of Buenos Aires according to the human density (HD) and economic level (EL) in the surroundings. HD and EL were considered as independent variables with three categories each. Twenty public squares (one hectare of surface) were randomly selected for each existing combination of the two independent variables. Ten random samples of fresh canine faeces were obtained in each square and analysed for helminths by the sedimentation and flotation techniques. The prevalence for each of the species was analysed using generalized linear models (GLM). The prevalence was modelled with a binomial error distribution and a logit link function. Helminth eggs were detected in 45 out of the 200 (22.5%) faecal samples collected and in 18 of the 20 green spaces sampled. The species observed were Ancylostoma caninum (13% of samples), Trichuris vulpis (8%) and Toxocara canis (4.5%). The GLM indicated that the prevalence of A. caninum in the slum areas (very high HD and very low EL) was higher than that in the other areas studied. However, the HD seemed to contribute more than the EL to the variations in the prevalence of A. caninum in faecal samples. The GLM showed no differences in the prevalence of the other parasite species for the different levels of the independent variables.

scholarly journals Phylogenetic and genetic characterization of Treponema pallidum strains from syphilis patients in Japan by whole-genome sequence analysis from global perspectives

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shingo Nishiki ◽  
Kenichi Lee ◽  
Mizue Kanai ◽  
Shu-ichi Nakayama ◽  
Makoto Ohnishi
Keyword(s):  
Genetic Difference ◽  
Phylogenetic Analyses ◽  
Treponema Pallidum ◽  
Whole Genome Sequence ◽  
Epidemiological Surveillance ◽  
Recent Common Ancestor ◽  
Whole Genome ◽  
Global Perspectives ◽  
Most Recent Common Ancestor ◽  
Sex With Men

AbstractJapan has had a substantial increase in syphilis cases since 2013. However, research on the genomic features of the Treponema pallidum subspecies pallidum (TPA) strains from these cases has been limited. Here, we elucidated the genetic variations and relationships between TPA strains in Japan (detected between 2014 and 2018) and other countries by whole-genome sequencing and phylogenetic analyses, including syphilis epidemiological surveillance data and information on patient sexual orientation. Seventeen of the 20 strains in Japan were SS14- and the remaining 3 were Nichols-lineage. Sixteen of the 17 SS14-lineage strains were classified into previously reported Sub-lineage 1B. Sub-lineage 1B strains in Japan have formed distinct sub-clusters of strains from heterosexuals and strains from men who have sex with men. These strains were closely related to reported TPA strains in China, forming an East-Asian cluster. However, those strains in these countries evolved independently after diverging from their most recent common ancestor and expanded their genetic diversity during the time of syphilis outbreak in each country. The genetic difference between the TPA strains in these countries was characterized by single-nucleotide-polymorphism analyses of their penicillin binding protein genes. Taken together, our results elucidated the detailed phylogenetic features and transmission networks of syphilis.

scholarly journals Research Note. Pearsonema plica and Eucoleus böhmi infections and associated lesions in wolves (Canis lupus) from Italy

2015 ◽  
Vol 52 (4) ◽  
pp. 364-369 ◽  
Author(s):  
A. Mariacher ◽  
C. Eleni ◽  
R. Fico ◽  
E. Ciarrocca ◽  
S. Perrucci
Keyword(s):  
Histological Examination ◽  
Canis Lupus ◽  
Nasal Bone ◽  
Research Note ◽  
Chronic Cystitis ◽  
First Report ◽  
Pathological Lesions ◽  
Eosinophilic Cystitis ◽  
Associated Lesions ◽  
Faecal Samples

Summary Reports of Pearsonema plica and Eucoleus böhmi infections in wolves (Canis lupus) in Europe are limited and data on associated lesions are lacking. In the present study urinary bladders, nasal turbinates and faecal samples from 8 necropsied wolves were examined for P. plica and E. böhmi infections and associated lesions. P. plica was identified in the bladder of four wolves. At histological examination, follicular chronic cystitis and eosinophilic cystitis were found. E. böhmi nematodes and eggs were identified from the nasal turbinates and rectal faecal samples of three wolves. Worms and eggs were found embedded in the mucosa among the nasal bone laminae. Two wolves were found coinfected by P. plica and E. böhmi. This is the first report of P. plica and E. böhmi infections in wolves from Italy and the first description of pathological lesions associated with P. plica infection in wolves.

Understanding the use of pulse oximetry in COVID-19 disease

2021 ◽  
Vol 32 (8) ◽  
pp. 312-316
Author(s):  
Paul Silverston
Keyword(s):  
Oxygen Saturation ◽  
Pulse Oximetry ◽  
Respiratory Symptoms ◽  
Disease Process ◽  
Clinical Findings ◽  
The Other ◽  
Know How ◽  
Non Invasive ◽  
Pulse Oximeters ◽  
The Way

The pandemic has led to an increase in the use of pulse oximetry to assess and manage patients with COVID-19 disease. Paul Silverston explains the principles of pulse oximetry and the factors that can affect the reliability and accuracy of readings Pulse oximetry is performed to detect and quantify the degree of hypoxia in patients with respiratory symptoms and illnesses, including patients with COVID-19 disease. Pulse oximeters are non-invasive, simple to use and inexpensive, but it is important to know how to interpret the readings in the context of the patient's symptoms and the other clinical findings. In COVID-19 disease, very small differences in the oxygen saturation reading result in significant differences in the way that the patient is managed, so it is important to be aware of the factors that can affect these readings. It is also important to appreciate that a low reading in a patient with suspected or confirmed COVID-19 disease may be the result of another disease process.

Anxiety of High School Students Preparing for University during the COVID-19 Pandemic

2021 ◽  
Vol 8 (12) ◽  
pp. 374-377
Author(s):  
Prapattra Hongwisat ◽  
Thanawat Wuthikanokkan ◽  
Nathakan Preechakansakul
Keyword(s):  
High School ◽  
High School Students ◽  
Respiratory Symptoms ◽  
The Other ◽  
Depression And Anxiety ◽  
School Students ◽  
Other Hand ◽  
The University ◽  
The Impact ◽  
Online Form

Covid-19 are one of the viruses that were widely spreaded in 2019 and are still separate until nowadays. Thailand is one of the countries that are highly infected. The majority of people who are infected with this virus tend to have mild to severe respiratory symptoms. Furthermore, anyone can get sick, and it can lead to death. However, the most common symptoms of this virus are fever, cough, tiredness, and loss of taste or smell, on the other hand, characteristics in a minority of people, such as diarrhea and headaches. Due to the impact of the Covid-19 virus, people have to change their lifestyle to the online form. These changes have impacted mostly on economics and education in particular countries, so this problem also affects anxiety among high school students; who must prepare to apply to the university during the COVID-19 outbreak. In addition, we have collected 151 answers from high school students by surveying in order to know the feelings for entrance to the university during the pandemic situation. We found out that 81.3% of the students are highly affected by covid, and only 0.7% of students are slightly affected. We also found out that 55% of the students are worried about university entrance, and only 2.6% of the students were not worried at all. According to the result, most of the students in Thailand are facing the problem about their education and their entrance for the university which are caused by Covid-19. This may lead to illnesses like depression and anxiety. Keywords: Students, Learning, COVID-19, Thailand, University.

Superconductive Properties of CsxRbyC60 (33K) - Isotope Effect -

1992 ◽  
Vol 247 ◽  
Author(s):  
T. W. Ebbesen ◽  
K. Tanigaki ◽  
S. Saito ◽  
J. Mizuki ◽  
J. S. Tsai ◽  
...  
Keyword(s):  
Fermi Level ◽  
Penetration Depth ◽  
Isotope Effect ◽  
Lattice Constant ◽  
Density Of States ◽  
Coherence Length ◽  
The Other ◽  
First Report ◽  
Electron Coupling

ABSTRACTThe surprisingly high Tc for the superconductivity of alkali doped C60 has spurred wide interest in understanding its mechanism [1–7]. We first report the superconductive properties of CsxRbyC60 which has a Tc as high as 33 K when x=2 and y=1 in the feed [4, 5]. SQUID measurements show that in this material the coherence length is 45 A and the penetration depth about 1, 800 A [5]. It has now been proven that the observed increase in the Tc with the size of the alkali dopant is due to the increase in the lattice constant [6]. This is most likely due to the changes in the density of states at the Fermi level. The other important parameter according to BSC theory is the phonon which mediates the electron-electron coupling. In the second part of this paper we present recent results which show that the Tc is indeed strongly influenced by this parameter [7]. The isotope effect is unexpectedly strong on the Tc.

scholarly journals External Quality Assessment in the Evaluation of Laboratory Performance of Faecal Culture

2017 ◽  
Vol 10 ◽  
pp. 117863611769125
Author(s):  
Salla J Kiiskinen ◽  
Tarja Ojanen ◽  
Yvonne Björkman ◽  
Harri Laitinen ◽  
Anja Siitonen
Keyword(s):  
Public Health ◽  
Quality Control ◽  
External Quality Assessment ◽  
False Negative ◽  
The Other ◽  
Epidemiological Surveillance ◽  
External Quality Control ◽  
External Quality ◽  
Faecal Culture

In Finland, all laboratories carrying out diagnostics of infectious diseases in humans are approved by the Regional State Administrative Agencies and are obligated to participate in External Quality Assurance rounds. Performance in these rounds is thought to reflect the quality of laboratory work. In the 6-year study period, 17 Finnish laboratories received 48 simulated faecal specimens for the culturing of diarrhoeal pathogens, yielding altogether 586 faecal culture External Quality Control specimens and 581 reports. The results were correct in 92% of all reports and in 67% of all specimens. False-negative Salmonella results were given for 2 of the 18 specimens, one with biochemically atypical Salmonella strain and the other with a low count of Salmonella cells. False-negative Shigella report was given for 6 of the 7 specimens in some participating laboratory. Detection of all common faecal pathogens is especially relevant to patient safety, public health, and epidemiological surveillance.

scholarly journals Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis.

2020 ◽  
Author(s):  
Manal Mohammed ◽  
Salina Thapa
Keyword(s):  
Salmonella Typhimurium ◽  
Multilocus Sequence Typing ◽  
Bacterial Infections ◽  
Outbreak Investigation ◽  
Whole Genome Sequence ◽  
Epidemiological Surveillance ◽  
Whole Genome ◽  
Salmonella Dublin ◽  
Source Of Infection ◽  
Investigation Methods

Abstract Background: Salmonellosis is one of the most common foodborne diseases worldwide. Although human infection by non-typhoidal Salmonella (NTS) enterica subspecies enterica is associated primarily with a self-limiting diarrhoeal illness, invasive bacterial infections (such as septicaemia, bacteraemia and meningitis) were also reported. Human outbreaks of NTS were reported in several countries all over the world including developing as well as high-income countries. Conventional laboratory methods such as pulsed field gel electrophoresis (PFGE) do not display adequate discrimination and have their limitations in epidemiological surveillance. It is therefore very crucial to use accurate, reliable and highly discriminative subtyping methods for epidemiological characterisation and outbreak investigation. Methods: Here, we used different whole genome sequence (WGS)-based subtyping methods for retrospective investigation of two different outbreaks of Salmonella Typhimurium and Salmonella Dublin that occurred in 2013 in UK and Ireland respectively. Results: Single nucleotide polymorphism (SNP)-based cluster analysis of Salmonella Typhimurium genomes revealed well supported clades, that were concordant with epidemiologically defined outbreak and confirmed the source of outbreak is due to consumption of contaminated mayonnaise. SNP-analyses of Salmonella Dublin genomes confirmed the outbreak however the source of infection could not be determined. The core genome multilocus sequence typing (cgMLST) was discriminatory and separated the outbreak strains of Salmonella Dublin from the non-outbreak strains that were concordant with the epidemiological data however cgMLST could neither discriminate between the outbreak and non-outbreak strains of Salmonella Typhimurium nor confirm that contaminated mayonnaise is the source of infection, On the other hand, other WGS-based subtyping methods including multilocus sequence typing (MLST), ribosomal MLST (rMLST), whole genome MLST (wgMLST), clustered regularly interspaced short palindromic repeats (CRISPRs), prophage sequence profiling, antibiotic resistance profile and plasmid typing methods were less discriminatory and could not confirm the source of the outbreak. Conclusions: Foodborne salmonellosis is an important concern for public health therefore, it is crucial to use accurate, reliable and highly discriminative subtyping methods for epidemiological surveillance and outbreak investigation. In this study, we showed that SNP-based analyses do not only have the ability to confirm the occurrence of the outbreak but also to provide definitive evidence of the source of the outbreak in real-time.

scholarly journals Two Cases of Hepatic Mastocytoma in Sheep

1972 ◽  
Vol 9 (2) ◽  
pp. 159-163 ◽  
Author(s):  
A. C. Johnstone
Keyword(s):  
Lymph Node ◽  
The Other ◽  
First Report ◽  
Hepatic Neoplasia

Two mastocytomas of the liver of sheep are described. The specimens were obtained during a survey of hepatic neoplasia that was conducted on abattoir-slaughtered sheep. Although tissues other than the liver were not examined, both tumours had metastasised, one to the portohepatic lymph node, and the other intrahepatically. It is believed that this is the first report of mastocytoma in this species.

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