Cold Shock Induced Oxidative Stress, Apoptosis and Genome-wide Gene Expression Perturbation in the Eyes of Zebrafish and the Mitigation of Blue Wavelength Light

Abstract As global climate changes, severely temperature variations have significant impacts on survival and development of fish. While the potential effects of light wavelength on cold shock and associated mechanisms remain largely unknown in fish. Here, zebrafish were pre-exposed to white LEDs (an irradiance of 0.9 W/m2) and blue LEDs (LDB, peak at 450 nm, 0.9 W/m2) for 2 weeks, and then exposed to 26℃ or 11℃ for 48 hours, respectively. Cold shock led to low survival rate. Cold shock altered retinal structure, increased the number of apoptotic cells and Caspase-3 activity, inhibited superoxide dismutase (SOD) and catalase (CAT) activities, up-regulated mRNA expression of (NF-E2-related factor 2) nrf2, p53, casp3 and casp9, and down-regulated cat expression in fish eyes. These results demonstrated that acute cold exposure induced oxidative stress and apoptosis in zebrafish eyes, which may lead to mortality. However, cold shock in combination with LDB apparently mitigated these negative effects, which might be involved in the up-regulation of antioxidant response and down-regulation of apoptotic responses at transcriptional and translational levels. Furthermore, cold shock also caused dysregulation of genome-wide gene expression involved in circadian rhythm, phototransduction and il-17 signaling pathway, indicating that cold shock disturbed phototransduction cascade and circadian rhythm signals and caused inflammatory responses. Ten key genes involved in circadian rhythm, phototransduction, cell cycle arrest, RNA processing or inflammatory responses were identified, including muc5d, rnps1, si:dkey-243i1.1, opn1mw1, gadd45ba, cebpd, btg2, si:dkey-242g16.2, nr1d1 and zgc:122979, which may play an important role in the protection of LDB against cold shock. Finally, our study suggested the relationship between spectrum and cold stress and demonstrated LDB could protect fish against the negative effect of cold stress in the eyes.

Download Full-text

Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcεRI-aggregation reveals a complex network of genes involved in inflammatory responses

BMC Genomics ◽

10.1186/1471-2164-7-210 ◽

2006 ◽

Vol 7 (1) ◽

Cited By ~ 36

Author(s):

Manikandan Jayapal ◽

Hwee Kee Tay ◽

Renji Reghunathan ◽

Liang Zhi ◽

Kah Kiong Chow ◽

...

Keyword(s):

Gene Expression ◽

Mast Cells ◽

Gene Expression Profiling ◽

Complex Network ◽

Expression Profiling ◽

Inflammatory Responses ◽

Human Mast Cells ◽

Genome Wide ◽

Genome Wide Gene Expression

Download Full-text

Integrative Analysis of Genome-wide Gene Expression for Prostate Cancer Prognosis

10.21236/ada552228 ◽

2011 ◽

Author(s):

Jiyoung Ahn

Keyword(s):

Gene Expression ◽

Prostate Cancer ◽

Integrative Analysis ◽

Cancer Prognosis ◽

Genome Wide ◽

Genome Wide Gene Expression ◽

Prostate Cancer Prognosis

Download Full-text

Genome-wide gene expression changes in postpartum depression point towards an altered immune landscape

Translational Psychiatry ◽

10.1038/s41398-021-01270-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Divya Mehta ◽

Karen Grewen ◽

Brenda Pearson ◽

Shivangi Wani ◽

Leanne Wallace ◽

...

Keyword(s):

Gene Expression ◽

Depressive Symptoms ◽

Postpartum Depression ◽

Expression Profiles ◽

Depression Scale ◽

Well Being ◽

Health Concern ◽

Genome Wide ◽

A Genome ◽

Genome Wide Gene Expression

AbstractMaternal postpartum depression (PPD) is a significant public health concern due to the severe negative impact on maternal and child health and well-being. In this study, we aimed to identify genes associated with PPD. To do this, we investigated genome-wide gene expression profiles of pregnant women during their third trimester of pregnancy and tested the association of gene expression with perinatal depressive symptoms. A total of 137 women from a cohort from the University of North Carolina, USA were assessed. The main phenotypes analysed were Edinburgh Postnatal Depression Scale (EPDS) scores at 2 months postpartum and PPD (binary yes/no) based on an EPDS cutoff of 10. Illumina NextSeq500/550 transcriptomic sequencing from whole blood was analysed using the edgeR package. We identified 71 genes significantly associated with postpartum depression scores at 2 months, after correction for multiple testing at 5% FDR. These included several interesting candidates including TNFRSF17, previously reported to be significantly upregulated in women with PPD and MMP8, a matrix metalloproteinase gene, associated with depression in a genome-wide association study. Functional annotation of differentially expressed genes revealed an enrichment of immune response-related biological processes. Additional analysis of genes associated with changes in depressive symptoms from recruitment to 2 months postpartum identified 66 genes significant at an FDR of 5%. Of these genes, 33 genes were also associated with depressive symptoms at 2 months postpartum. Comparing the results with previous studies, we observed that 15.4% of genes associated with PPD in this study overlapped with 700 core maternal genes that showed significant gene expression changes across multiple brain regions (P = 7.9e-05) and 29–53% of the genes were also associated with estradiol changes in a pharmacological model of depression (P values range = 1.2e-4–2.1e-14). In conclusion, we identified novel genes and validated genes previously associated with oestrogen sensitivity in PPD. These results point towards the role of an altered immune transcriptomic landscape as a vulnerability factor for PPD.

Download Full-text

Arsenic hexoxide has differential effects on cell proliferation and genome-wide gene expression in human primary mammary epithelial and MCF7 cells

Scientific Reports ◽

10.1038/s41598-021-82551-3 ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Donguk Kim ◽

Na Yeon Park ◽

Keunsoo Kang ◽

Stuart K. Calderwood ◽

Dong-Hyung Cho ◽

...

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Cancer Cells ◽

Mammary Epithelial ◽

Mcf7 Cells ◽

Differential Effects ◽

Cycle Arrest ◽

Genome Wide ◽

Human Mammary Epithelial ◽

Genome Wide Gene Expression

AbstractArsenic is reportedly a biphasic inorganic compound for its toxicity and anticancer effects in humans. Recent studies have shown that certain arsenic compounds including arsenic hexoxide (AS4O6; hereafter, AS6) induce programmed cell death and cell cycle arrest in human cancer cells and murine cancer models. However, the mechanisms by which AS6 suppresses cancer cells are incompletely understood. In this study, we report the mechanisms of AS6 through transcriptome analyses. In particular, the cytotoxicity and global gene expression regulation by AS6 were compared in human normal and cancer breast epithelial cells. Using RNA-sequencing and bioinformatics analyses, differentially expressed genes in significantly affected biological pathways in these cell types were validated by real-time quantitative polymerase chain reaction and immunoblotting assays. Our data show markedly differential effects of AS6 on cytotoxicity and gene expression in human mammary epithelial normal cells (HUMEC) and Michigan Cancer Foundation 7 (MCF7), a human mammary epithelial cancer cell line. AS6 selectively arrests cell growth and induces cell death in MCF7 cells without affecting the growth of HUMEC in a dose-dependent manner. AS6 alters the transcription of a large number of genes in MCF7 cells, but much fewer genes in HUMEC. Importantly, we found that the cell proliferation, cell cycle, and DNA repair pathways are significantly suppressed whereas cellular stress response and apoptotic pathways increase in AS6-treated MCF7 cells. Together, we provide the first evidence of differential effects of AS6 on normal and cancerous breast epithelial cells, suggesting that AS6 at moderate concentrations induces cell cycle arrest and apoptosis through modulating genome-wide gene expression, leading to compromised DNA repair and increased genome instability selectively in human breast cancer cells.

Download Full-text

Modular genome-wide gene expression architecture shared by early traits of osteoporosis and atherosclerosis in the Young Finns Study

Scientific Reports ◽

10.1038/s41598-021-86536-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Binisha H. Mishra ◽

Pashupati P. Mishra ◽

Emma Raitoharju ◽

Saara Marttila ◽

Nina Mononen ◽

...

Keyword(s):

Gene Expression ◽

Smoking Habit ◽

Intima Media Thickness ◽

Mineral Density ◽

Genome Wide ◽

Study Support ◽

Gene Modules ◽

Study Participants ◽

Genome Wide Gene Expression ◽

Young Finns Study

AbstractWe analysed whole blood genome-wide expression data to identify gene co-expression modules shared by early traits of osteoporosis and atherosclerosis. Gene expression was profiled for the Young Finns Study participants. Bone mineral density and content were measured as early traits of osteoporosis. Carotid and bulbus intima media thickness were measured as early traits of atherosclerosis. Joint association of the modules, identified with weighted co-expression analysis, with early traits of the diseases was tested with multivariate analysis. Among the six modules significantly correlated with early traits of both the diseases, two had significant (adjusted p-values (p.adj) < 0.05) and another two had suggestively significant (p.adj < 0.25) joint association with the two diseases after adjusting for age, sex, body mass index, smoking habit, alcohol consumption, and physical activity. The three most significant member genes from the significant modules were NOSIP, GXYLT2, and TRIM63 (p.adj ≤ 0.18). Genes in the modules were enriched with biological processes that have separately been found to be involved in either bone metabolism or atherosclerosis. The gene modules and their most significant member genes identified in this study support the osteoporosis-atherosclerosis comorbidity hypothesis and can provide new joint biomarkers for both diseases and their dual prevention.

Download Full-text

Camu-Camu Fruit Extract Inhibits Oxidative Stress and Inflammatory Responses by Regulating NFAT and Nrf2 Signaling Pathways in High Glucose-Induced Human Keratinocytes

Molecules ◽

10.3390/molecules26113174 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3174

Author(s):

Nhung Quynh Do ◽

Shengdao Zheng ◽

Bom Park ◽

Quynh T. N. Nguyen ◽

Bo-Ram Choi ◽

...

Keyword(s):

Oxidative Stress ◽

Signaling Pathways ◽

High Glucose ◽

Skin Diseases ◽

Inflammatory Responses ◽

Human Keratinocytes ◽

Related Factor ◽

Nuclear Factor ◽

Fruit Extract ◽

Activated T Cells

Myrciaria dubia (HBK) McVaugh (camu-camu) belongs to the family Myrtaceae. Although camu-camu has received a great deal of attention for its potential pharmacological activities, there is little information on the anti-oxidative stress and anti-inflammatory effects of camu-camu fruit in skin diseases. In the present study, we investigated the preventative effect of 70% ethanol camu-camu fruit extract against high glucose-induced human keratinocytes. High glucose-induced overproduction of reactive oxygen species (ROS) was inhibited by camu-camu fruit treatment. In response to ROS reduction, camu-camu fruit modulated the mitogen-activated protein kinases (MAPK)/activator protein-1 (AP-1), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and nuclear factor of activated T cells (NFAT) signaling pathways related to inflammation by downregulating the expression of proinflammatory cytokines and chemokines. Furthermore, camu-camu fruit treatment activated the expression of nuclear factor E2-related factor 2 (Nrf2) and subsequently increased the NAD(P)H:quinone oxidoreductase1 (NQO1) expression to protect keratinocytes against high-glucose-induced oxidative stress. These results indicate that camu-camu fruit is a promising material for preventing oxidative stress and skin inflammation induced by high glucose level.

Download Full-text