scholarly journals Separate and Combined Effects of Chlortetracycline and Oxytetracycline on Growth and Antioxidant Enzymes in Myriophyllum Aquaticum

2021 ◽  
Author(s):  
Jing Li ◽  
Zhonghua Wu
Keyword(s):  
Antioxidant Enzymes ◽  
Chlorophyll Content ◽  
Photosynthetic Pigment ◽  
Total Chlorophyll ◽  
Combined Treatments ◽  
Combined Toxicity ◽  
Myriophyllum Aquaticum ◽  
Total Chlorophyll Content ◽  
Protective Enzyme ◽  
Mda Content

Abstract As two typical tetracyclines, chlortetracycline (CTC) and oxytetracycline (OTC) coexist widely in water. In the experiment, Myriophyllum aquaticum (Vell.) Verdc was exposed to the orthogonal hydroponic environment with the concentrations of CTC and OTC at 1, 10, and 50 mg/L for 7 days (7 D) and 14 days (14 D). The results showed that the plant height, relative growth rates (RGR), and photosynthetic pigment contents of M. aquaticum decreased significantly after 14 D of CTC/ OTC, which was stronger than that after 7 D, and the toxicity of CTC was stronger than OTC. The combined toxicity of the two on the RGR and total chlorophyll content was mainly synergistic or additive after 7 D. After 14 D, the interaction changed to antagonism or addition, and the response of total chlorophyll content was more obvious than that of RGR. After 7 D of combined treatments, the malondialdehyde (MDA) content decreased significantly, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities responded to different combined concentrations, respectively, and CAT was the main protective enzyme of M. aquaticum. After 14 D of combined treatments, the hydrogen peroxide (H2O2) content increased significantly, the MDA content increased first and then decreased, and POD was the main protective enzyme among the three antioxidant enzymes. Overall, this study provided the first insight into the single and combined toxicity of CTC and OTC on the growth and physiology of M. aquaticum at different time periods, revealing the reversal of the combined toxicity with time of exposure.

scholarly journals Response of Solanum melongena L. to Inoculation with Arbuscular Mycorrhizal Fungi under Low and High Phosphate Condition

2011 ◽  
Vol 3 (3) ◽  
pp. 70-74 ◽  
Author(s):  
Irfan AZIZ ◽  
Mohd AYOOB ◽  
Paramjit Kaur JITE
Keyword(s):  
Alkaline Phosphatase ◽  
Antioxidant Enzymes ◽  
Chlorophyll Content ◽  
Phosphatase Activity ◽  
Solanum Melongena ◽  
Arbuscular Mycorrhizal ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Acid And Alkaline Phosphatase ◽  
High Phosphate

Solanum melongena L. a medicinally and economically important crop plants were grown in polythene bags. The effect of mycorrhizal inoculation (Glomus mosseae) and increasing phosphate levels on the expression of the photosynthetic activity in terms of chlorophyll content. Antioxidant enzymes like peroxidase, polyphenol oxidase, root acid and alkaline phosphatase activity of Solanum melongena were evaluated. The experimental design was entirely at CRBD with eight treatments with three levels of phosphate (50,100,150 mg kg-1 of soil). Root colonization ranged from 50.33% to 67.33% . The activity of the studied antioxidant enzymes were found to be increased in arbuscular mycorrhizal (AM) Solanum plants. Root phosphatase activity was greater in 100 and 150 mg phosphate level in AM treated than non AM treated Solanum plants. Besides, only AM treated plants of Solanum reflected increase in total chlorophyll content as compared to non AM plants. This work suggests that the mycorrhiza helps Solanum plants to perform better in low and high phosphate level by enhancing antioxidant enzyme activity, acid and alkaline phosphatase activity and total chlorophyll content.

scholarly journals Improving the Keeping Quality and Vase Life of Cut Alstroemeria Flowers by Pre and Post-harvest Salicylic Acid Treatments

2013 ◽  
Vol 5 (3) ◽  
pp. 364-370 ◽  
Author(s):  
Elnaz SOLEIMANY-FARD ◽  
Khodayar HEMMATI ◽  
Ahmad KHALIGHI
Keyword(s):  
Salicylic Acid ◽  
Water Balance ◽  
Chlorophyll Content ◽  
Water Loss ◽  
Vase Life ◽  
Total Chlorophyll ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Total Chlorophyll Content ◽  
Keeping Quality

Keeping quality and length of vase life are important factors for evaluation of cut flowers quality, for both domestic and export markets. Studding the effect of pre- and post-harvest salicylic acid applications on keeping quality and vase life of cut alstroemeria flowers during vase period is the approach taken. Aqueous solutions of salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM were sprayed to run-off (approximately 500 mL per plant), about two weeks before flowers harvest. The cut flowers were harvested in the early morning and both of cut flowers treated (sprayed) and untreated were kept in vase solutions containing salicylic acid at 0.0 (with distilled water), 1, 2 and 3 mM. Sucrose at 4% was added to all treatments as a base solution. The changes in relative fresh weight, water uptake, water loss, water balance, total chlorophyll content and vase life were estimated during vase period. The results showed that the relative fresh weight, water uptake, water balance, total chlorophyll content and vase life decreased significantly while the water loss increased significantly during experiment for all treatments. A significant difference between salicylic acid and control treatments in all measured parameters is observed. During vase period, the salicylic acid treatments maintained significantly a more favourable relative fresh weight, water uptake, water balance, total chlorophyll content and supressed significantly water loss, as compared to control treatment. Also, the results showed that the using salicylic acid increased significantly the vase life cut alstroemeria flowers, over control. The highest values of measured parameters were found when plants were treated by pre + post-harvest application of salicylic acid at 3 mM. The result revealed that the quality attributes and vase life of cut alstroemeria flowers were improved by the use of salicylic acid treatment.

scholarly journals Antioxidant gene expression, chlorophyll, and starch content in Persian lime (Citrus latifolia Tanaka Ex Q. Jiménez) trees with HLB by application of elicitors of plant resistance

2021 ◽  
Vol 6 (2) ◽  
pp. 86-102
Author(s):  
Felipe Roberto Flores-de la Rosa ◽  
◽  
Ricardo Santillán-Mendoza ◽  
Cynthia Guadalupe Rodríguez-Quibrera ◽  
Antonio Martínez-Ruiz ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Starch Content ◽  
Starch Accumulation ◽  
Economic Losses ◽  
Total Chlorophyll ◽  
Gamma Aminobutyric Acid ◽  
Antioxidant Genes ◽  
Spectrophotometric Methods ◽  
Total Chlorophyll Content ◽  
Randomized Design

Persian lime is economically important for Mexico. However, the disease known as Huanglongbing (HLB) causes chlorosis symptoms in the foliage that affect the growth and decreases the yield of the trees. The expression of chlorosis symptoms is associated with starch accumulation and inhibition of antioxidant activity. The objective of the present work was to determine the effect of the application of three resistance elicitors on the expression of three antioxidant genes and on starch and chlorophyll content in Persian lime trees with HLB. Salicylic acid (SA), jasmonic acid (JA) or gamma-aminobutyric acid (GABA) were applied every eight days for nine weeks using a completely randomized design and a one-way ANOVA was performed for the analysis. Expression of APX, CAT and SOD genes was quantified at week nine at four different times. Starch and total chlorophyll content was estimated every three weeks by spectrophotometric methods. The application of the elicitors significantly increased the expression of the three genes, with SA and GABA generating the greatest increase at different times of application. No difference was found in starch and total chlorophyll content at most of the times evaluated. The use of resistance elicitors is promising in the management of HLB in plots already infected, seeking to extend the productive life of the orchards and thus counteract the economic losses caused by HLB.

scholarly journals PENGARUH PERBANDINGAN IKAN KEMBUNG (Rastrelliger kanagurta) DENGAN SAYUR GONDA (Sphenoclea zeylanica Gaertner) TERHADAP KARAKTERISTIK BAKSO

2020 ◽  
Vol 9 (4) ◽  
pp. 426
Author(s):  
Brogina Mayank Dini ◽  
Luh Putu Trisna Darmayanti ◽  
I Ketut Suter
Keyword(s):  
Antioxidant Capacity ◽  
Protein Content ◽  
Water Content ◽  
Chlorophyll Content ◽  
Fat Content ◽  
Ash Content ◽  
Carbohydrate Content ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Randomized Design

This research was conducted with the aim to determine the effect of comparison of mackerel with gonda vegetable on meatballs characteristics and get a comparison of mackerel with specific gonda vegetable that can produce meatballs with the best characteristics. This study uses a Completely Randomized Design (CRD) with treatment that is the comparison of mackerel with gonda vegetable consisting of 6 levels: 100%: 0%; 95%: 5%; 90%: 10%; 85%: 15%; 80%: 20%; 75%: 25%. Each treatment was repeated 3 times so that obtained 18 units. The data is then analyzed by analysis of variance and if the treatment influences the proposed variable then it is continued by Duncan. The results showed that the comparison of mackerel and gonda vegetable had a very significant effect on water content, ash content, fat content, protein content, carbohydrate content, total chlorophyll content, antioxidant capacity, color (scoring), texture, aroma, overall acceptance and no significant effect for texture, aroma and taste. Comparison of 80% mackerel and 20% gonda vegetables had the best characteristics, with 62.99% water content, 1.56% ash content, 1.28% fat content, 8.75% protein content, 25.43% carbohydrate content, 18.39% total chlorophyll content, antioxidant capacity 25.63%. Sensory properties obtained are color (rather green, rather like), texture (rather like), flavor (liked) and overall acceptance (like).

scholarly journals The Effect of Organic Fertilizers on Growth and Yield of Water Spinach (Ipomoea reptans Poir)

2021 ◽  
Vol 3 (2) ◽  
pp. 37-44
Author(s):  
Selvia Dewi Pohan
Keyword(s):  
Plant Growth ◽  
Water Content ◽  
Chlorophyll Content ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Total Chlorophyll ◽  
Cow Manure ◽  
Water Spinach ◽  
Total Chlorophyll Content

Water spinach (Ipomoea reptans Poir) had been identified as a nutritious vegetable with high demand in Indonesia. Besides, this plant also has been evidenced to play an important role in environmental cleaning as phytoremediator. The study about the effect of organic fertilizers on the growth and yield of water spinach (Ipomoea reptans Poir) has been conducted in the Green House of Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan. The study aims to investigate the most effective type of fertilizers and the dose for water spinach’s (Ipomoea reptans Poir) growth and yield. A Completely Randomized Factorial Design was designed for the experiment with two factors and three repetitions. The first factor is the type of fertilizer (chicken manure, cow manure, and compost), and the second factor is the dose of the fertilizer (1:1, 2:1, and 3:1). The parameters such as plant height, number of shoots, number of leaves, fresh weight, dry weight, leaf’s total chlorophyll content, and water content were measured to evaluate plant growth and yield. The General Linear Model used SPSS 21 programs was applied to analyze the collected data. Study results revealed that cow manure increased plant growth and yield significantly with dose 2:1 as the finest treatment, followed by dose 3:1. Cow manure also increased total chlorophyll content (8.0574c mg. L-1), with the most suitable dose was 2:1 (8.2807 mg. L-1). The plant’s water content tended to be high in chicken manure (93%), and the lower water content was in cow manure with dose 3:1 (87.5%).

Cell membrane stability and chlorophyll content variation in wheat (Triticum aestivum) genotypes under conditions of heat and drought

2016 ◽  
Vol 67 (7) ◽  
pp. 712 ◽  
Author(s):  
Shoaib Ur Rehman ◽  
Muhammad Bilal ◽  
Rashid Mehmood Rana ◽  
Muhammad Naveed Tahir ◽  
Muhammad Kausar Nawaz Shah ◽  
...  
Keyword(s):  
Heat Stress ◽  
Drought Stress ◽  
Cell Membrane ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Stress Conditions ◽  
Seedling Stage ◽  
Total Chlorophyll ◽  
Cell Membrane Stability ◽  
Total Chlorophyll Content

Heat and drought are among the major obstacles confronting crop production under climate change. The present study was conducted to evaluate 50 diverse wheat genotypes for cell membrane stability (CMS) and chlorophyll content at seedling and anthesis stages under heat and drought stress conditions, to understand the effect of the two abiotic factors and to find promising genotypes for future breeding. Experiments were conducted in the glasshouse (seedling stage) and the field (anthesis stage). Analysis of variance showed significant variation (P ≤ 0.05) for all of the traits at seedling and anthesis stages. High levels of broad-sense heritability and genetic advance at 5% selection intensity indicated the presence of a high genetic component of variation and potential for genetic improvement through selection among the existing genetic variation. CMS showed a significant positive correlation with 1000-grain weight (TGW) under heat and drought conditions at both seedling and anthesis stages. Chlorophyll a/b ratio at seedling stage exhibited a significant negative correlation (r = –0.39, P < 0.05) with TGW under heat stress. Total chlorophyll content was significantly (r = 0.42, P < 0.05) correlated with TGW under heat stress at anthesis. Genotypes ETAD248 and ETAD7 showed the highest CMS and TGW values, whereas their chlorophyll a/b values were lowest, at both seedling and anthesis stages under heat and drought stress conditions. Higher CMS and total chlorophyll content, and lower chlorophyll a/b, were found to be useful indicators to identify genotypes with high TGW under heat and drought stress conditions. This study indicated the possibility of using seedling resistance as an indicator for later stage response in breeding for heat and drought resistance. The resistant genotypes identified can be used as potential germplasm in breeding programs.

scholarly journals Cultivation of Cress Involving Water Treated Under Different Atmospheres with Low-Temperature, Low-Pressure Glow Plasma of Low Frequency

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2152
Author(s):  
Katarzyna Ciesielska ◽  
Wojciech Ciesielski ◽  
Damian Kulawik ◽  
Zdzisław Oszczęda ◽  
Piotr Tomasik
Keyword(s):  
Ascorbic Acid ◽  
Low Temperature ◽  
Chlorophyll Content ◽  
Tap Water ◽  
Low Frequency ◽  
Low Pressure ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Glow Plasma ◽  
Water Saturated

Watering cress with tap water treated for 30 min with low-temperature, low-pressure glow plasma of low frequency (LPGP) in the air (LPGPA), saturated with either nitrogen (LPGPN), CO2 (LPGPC) or methane (LPGPM), promoted the yield of crops. Their efficiency increased in the order LPGPA < LPGPN < LPGPM < LPGPC. The kind of water prior and after the treatment specifically influenced the fat and protein content in the watered cress and had no effect on the carbohydrate content. Watering cress with water saturated with N2 and CO2 slightly increased the total chlorophyll content, whereas watering with water saturated with CH4 significantly decreased it. Watering with plasma-treated water always resulted in an increase in the total chlorophyll content and subtly influenced the content of carotenoids and ascorbic acid. Watering cress with water saturated with particular gases influenced the bioaccumulation of cations and anions.

Total chlorophyll, growth and productivity of soybean [Glycine Max (L) Merril] under different levels and sources of phosphorus and plant growth regulators in south Rajasthan, India

2016 ◽  
Author(s):  
Shankar Lal Khaswan ◽  
R. K. Dubey ◽  
R. C. Tiwari ◽  
S. K. Dubey ◽  
Raj Kumari Chaudhary
Keyword(s):  
Plant Growth ◽  
Chlorophyll Content ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Total Biomass ◽  
Total Chlorophyll ◽  
Water Spray ◽  
Benzyl Adenine ◽  
Total Chlorophyll Content ◽  
Different Levels

An experiment was conducted at the Instructional Farm of Rajasthan College of Agriculture, Udaipur during Kharif 2009 and 2010 on medium clay loam soils to assess the influence of different levels and sources of phosphorus fertilization as well as plant growth regulators on productivity of soybean. Twenty seven treatments i.e. three levels (20, 30 and 40 kg P2O5 ha-1) and three sources (Single super phosphate or SSP; phosphorus rich organic manure or PROM and di-ammonium phosphate or DAP) of phosphorus in main plots and three PGRs (water spray, benzyl adenine 50 ppm and NAA 100 ppm) in sub plots of a split plot design having three replications. Results revealed that application of 40 kg P2O5 ha-1 recorded significantly higher pooled yield (q ha-1) of grain (25.95), stover (37.34) and total biomass (63.29); crop growth rate or CGR (g m-2day-1) between 30-60 days after sowing ( DAS)(18.83) and 61-90 DAS (11.96); total chlorophyll content at 45 DAS (2.26%) and 60 DAS (2.80%); Leaf area index(LAI) at 45 DAS (1.56) and 60 DAS (3.44) and green leaves plant-1 at 45 DAS (11.98) and 60 DAS (21.43). Among different phosphorus sources, SSP outperformed DAP and PROM on pooled yield (q ha-1) of grain (25.12), stover (36.23) and total biomass (61.35); CGR (g m-2day-1) between 30-60 DAS (11.22) and 61-90 DAS (10.94); total chlorophyll content at 45 DAS (2.26%) and 60 DAS (2.76%); LAI at 45 DAS (1.51) and 60 DAS (3.42) and green leaves plant-1 at 45 DAS (11.77) and 60 DAS (21.35). Foliar application of NAA recorded significantly higher pooled yield (q ha-1) of grain (24.23), stover (35.73) and total biomass (60.01) than benzyl adenine and water spray. However, variations recorded under NAA and benzyl adenine in CGR, total chlorophyll content, LAI and green leave plant-1at different stages of soybean crop were statistically at par.

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