scholarly journals Cellulase Production by Penicillium Oxalicum Ti-11 with Traditional Chinese Medicine Residue as Substrate

2021 ◽  
Author(s):  
xiaoxi zeng ◽  
yuanke zhang ◽  
lulu Chen ◽  
song Ran ◽  
cheng Zhang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Enzymatic Hydrolysis ◽  
Traditional Chinese Medicine ◽  
Enzyme Production ◽  
Cellulase Production ◽  
Waste Utilization ◽  
Before And After ◽  
Leaf Residue ◽  
Fungal Cellulase ◽  
Production Conditions

Abstract PurposeThe study aims to search for efficient cellulase producer and explore the possibility of traditional Chinese medicine residue as a substrate for cellulase production, so as to realize the waste utilization of traditional Chinese medicine residue.MethodsThe cellulase-producing strain was identified through morphological and molecular biological methods. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to characterize the structure of traditional Chinese medicine residues before and after fermentation. The enzyme activity was determined by DNS method, and the enzyme production conditions were optimized by single factor and response surface methodology.ResultThe strain grew well in forsythia leaf residue, and the highest FPA could reach 2.06 IU/mL. In addition, the structural characteristics of traditional Chinese medicine residue that before and after enzymatic hydrolysis were characterized by SEM and FTIR. The results showed that the structure of the residue was destroyed after enzymatic hydrolysis, the damage of forsythia leaf residue was the most serious, and enzymatic hydrolysis promoted the dissolution of cellulose, lignin and hemicellulose. The enzyme production conditions of the strain were optimized by Plackett-Burman design and response surface analysis. The FPA could reach 2.79 IU/mL under the optimal conditions of FLR concentration 24.84 g/L, (NH4)2SO4 concentration 2 g/L, temperature 34.44℃, pH 6.20, rotational speed 200rpm, inoculum 6%, which was 35.44% higher than that before optimization.ConclusionsThe results showed that traditional Chinese medicine residue could be used as the induced substrate for fungal cellulase production. This study provides an idea for the low-cost production of fungal cellulase and the waste utilization of traditional Chinese medicine residue.

scholarly journals Effects of Acupuncture on the Hypothalamus-Pituitary-Adrenal Axis in Chronic Insomnia Patients: A Study Protocol for a Randomized Controlled Trial

2019 ◽  
Author(s):  
Chengyong Liu ◽  
Shiyu Zheng ◽  
Wenzhong Wu ◽  
Xiaoqiu Wang ◽  
Shan Qin ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Outcome Measures ◽  
Controlled Trial ◽  
Severity Index ◽  
Chronic Insomnia ◽  
Secondary Outcome ◽  
Therapeutic Effects ◽  
Before And After ◽  
Fatigue Severity

Abstract Background : Acupuncture, as an important component of traditional Chinese medicine (TCM), has been widely applied in the treatment of chronic insomnia in China，while there is no clinical study related to its therapeutic mechanism. Methods/design : A single-center, single-blind, randomized, placebo-controlled trial will be conducted at Jiangsu Hospital of Traditional Chinese Medicine. A total of 60 patients will be registered.Eligible participantswill be randomly divided into acupuncture group and shamacupuncture group (n = 30 cases in each group). Patients in both groups will be treated once every other day, 3 times per week for 4 weeks.The primary outcome measures are Pittsburgh Sleep Quality Index (PSQI), concentrations of adrenocorticotropic hormone (ATCH), Corticotrophin-releasing hormone (CRH), and cortisol (CORT). Secondary outcome measures are Insomnia Severity Index (ISI) and Fatigue Severity Scale (FSS). Discussion : This study aims to evaluate the therapeutic effects of acupuncture on chronic insomnia by using PSQI, ISI, and FSS. The mechanism of acupuncture on CIPs will be preliminarily discussed by analyzing the changes in concentrations of CRH, ACTH, and CORT before and after treatment.

Expressions of IFN-γ and IL-4 before and after Treatment of Lupus Nephritis with Traditional Chinese Medicine Combined with Cyclophosphamide and Their Values for Efficacy Prediction and Evaluation

2020 ◽  
Author(s):  
Yan JIANG ◽  
Qichang ZHANG ◽  
Haoyu WANG ◽  
Dabei TANG ◽  
Yan ZHANG ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Lupus Nephritis ◽  
Clinical Efficacy ◽  
Activity Index ◽  
Interleukin 4 ◽  
Control Group ◽  
Before And After ◽  
Ifn Γ ◽  
Efficacy Prediction

Background: To explore IFN-γ (interferon-γ) and IL-4 (interleukin-4) expressions before and after the treatment of LN (lupus nephritis) and their values for efficacy prediction and evaluation. Methods: Altogether 107 patients with LN treated in the First Hospital of Qiqihaer City, Qiqihar, China from March 2017 to September 2018 were enrolled. Sixty-two patients were treated with cyclophosphamide and prednisolone (control group), while another 45 patients were treated with Qing Shen Fang based on the control group (observation group). Their clinical efficacy and changes in immune indices after treatment were observed. Results: Compared with those in the control group, clinical efficacy, IFN-γ, IL-4, hemoglobin, complements C3 and C4, ESR (erythrocyte sedimentation rate), serum IgG, SLEDAI (Systemic Lupus Erythematosus Disease Activity Index) score, and TCMSSS (Traditional Chinese Medicine Syndrome Score Scale) score were significantly improved after treatment in the study group. Based on the observation, IFN-γ and IL-4 could be used as potential indicators for evaluating clinical efficacy. Conclusion: The combination of cyclophosphamide, prednisolone, and Qing Shen Fang improves conditions of patients with LN and significantly reduces their IFN-γ and IL-4 levels in serum. IFN-γ and IL-4 can be used as potential indicators for the efficacy prediction and evaluation of the disease.

Economic Analysis on Utilization of Waste as Resources in Industrializing of Traditional Chinese Medicine Resources

2014 ◽  
Vol 675-677 ◽  
pp. 1838-1841
Author(s):  
Yu Fen Wang
Keyword(s):  
Sustainable Development ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Economic Analysis ◽  
Public Goods ◽  
Environmental Protection ◽  
Resource Utilization ◽  
Theoretical Basis ◽  
Waste Utilization ◽  
Practical Significance

To achieve sustainable development of Traditional Chinese medicine (TCM) industry we should focus on coordination of resource utilization and environmental protection and carry out utilization of waste as resources in the course of industrialization of TCM resources. The author analyzes the production of waste and the practical significance of waste utilization as resources in the industrialization. Economic analysis is on utilization of waste as resources with four economics principles of cyclic economy, public goods, theory of externality and theory of property right. The analysis provides theoretical basis, causes and a solution to change waste of TCM into resources.

scholarly journals Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Hui Du ◽  
Yonghong Wang ◽  
Yumin Shi ◽  
Jian Yu ◽  
Wen Sun ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Mrna Expression ◽  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Transforming Growth Factor ◽  
Pediatric Asthma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Before And After

Objective. To observe the effects of empirical prescriptions of traditional Chinese medicine (TCM) on inflammatory mediators in pediatric asthma and to explore the underlying molecular mechanism in the treatment of asthma.Methods. A total of 182 children with asthma were randomly placed into either the TCM group (n=97) or the salbutamol and montelukast (SM) group (n=85). Patients in the TCM group were treated with a series of empirical prescriptions of TCM, while those in the SM group received salbutamol and montelukast. Both groups received their respective treatment for 12 weeks. There were 35 patients in TCM group and 34 patients in SM group providing venous blood. Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factorβ1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. Enzyme-linked immunosorbent assay was used to measure the levels of IL-10, IL-17, MMP-9, and TGF-β1 in peripheral blood before and after treatment.Results. The mRNA expression of TGF-β1 in the SM group was downregulated (P=0.00) after treatment. No significant differences were found between the TCM group and the SM group after treatment (P>0.05). In the TCM group, the levels of IL-10, IL-17, and MMP-9 significantly decreased after treatment (P=0.01, 0.04, and 0.03, resp.). In the SM group, IL-17, MMP-9, and TGF-β1 levels significantly decreased after treatment (P=0.00, 0.03, and 0.00, resp.). There was no significant difference between the two groups regarding the levels of IL-10, IL-17, TGF-β1, and MMP-9 (P>0.05). The difference of the level of IL-17 was negatively correlated with the change of C-ACT score in TCM group and SM group.Conclusion. TCM has a regulatory effect on the balance of some inflammatory mediators in pediatric asthma.

TREATING PEDIATRIC ASTHMA WITH HOLISTIC APPROACHES OF TRADITIONAL CHINESE MEDICINE: A RETROSPECTIVE STUDY

2017 ◽  
Vol 15 (1) ◽  
pp. 142
Author(s):  
Shun-Chang Chang ◽  
Tsung-Chieh Lee ◽  
Chia-Ying Lee ◽  
Tsung-Lin Cheng ◽  
Mao-Feng Sun ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Pediatric Asthma ◽  
Admission Rate ◽  
Emergency Department Visits ◽  
Holistic Treatment ◽  
Before And After ◽  
Changhua Christian Hospital ◽  
Christian Hospital

Background: Asthma is a chronic disease increasingly found in children. To find more economical and efficient alternatives to treat pediatric asthma, the Bureau of National Health Insurance of Taiwan launched the Traditional Chinese Medicine Holistic Treatment Program (TCMHTP). The effect of traditional Chinese medicine (TCM) holistic treatments on pediatric asthma was evaluated based on data collected from the program. Materials and Methods: A retrospective study was performed by analyzing a dataset from Changhua Christian Hospital, Taiwan, between January 1st, 2006 and December 31st, 2010. Patients aged between 2 and 15 years, who had been diagnosed with asthma, and had participated in the TCMHTP were recruited, whereas those with other severe diseases were excluded. We analyzed the frequency of emergency department visits (EDV), inpatient admission rate (IAR), and length of hospitalization (LH) of the patients, before and after TCM treatments. Spectral analysis of heart rate variability (HRV) was also conducted. Results: Fifty-eight patients were recruited. The average age of the patients receiving TCM treatments was 5.67±3.03 years. The frequency of EDV decreased from 0.94±0.85 to 0.67±1.19 times annually (p=0.095), the annual IAR decreased from 0.62±0.78 to 0.26±0.67 (p=0.002) and the average LH decreased from 3.32±4.25 to 0.80±1.64 (p=0.000) days per year. Parasympathetically mediated HRV decreased significantly from 60.42±15.33 to 54.89±16.45 nu (p=0.016). Conclusion: The present study revealed that an appropriate period of TCM holistic treatment intervention can not only significantly lower exacerbations and hospitalization frequency but also reduce vagal tone in asthmatic children.

Normalized Acquisition System of the Facial Diagnosis in Traditional Chinese Medicine

2013 ◽  
Vol 291-294 ◽  
pp. 2834-2844
Author(s):  
Fu Feng Li ◽  
Peng Qian ◽  
Xiao Yan Zheng ◽  
Yi Qin Wang ◽  
Zhu Mei Sun ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Information Acquisition ◽  
Light Emitting Diode ◽  
Image Acquisition ◽  
Objective Evaluation ◽  
Acquisition System ◽  
Natural Light ◽  
Before And After ◽  
Standard Color

Background: Facial diagnosis, an important part of clinical diagnosis in Traditional Chinese Medicine (TCM), is a method used to diagnose the functions of Zang-Fu organs by observing the color, luster, shape, and texture of faces. However, the traditional facial diagnosis mainly relies on doctors’ eyes, languages, and personal clinical experiences. Results are not only determined based on the doctors’ diagnostic skills but also by external conditions such as light and temperature. Without objective evaluation criteria, conducting studies on facial diagnosis to widen its application are difficult. To solve this problem, we should find new methods and technologies to realize the objectification and normalization of diagnosis in TCM. In this article, we discuss the results of our study on the normalized acquisition system of facial diagnosis in TCM. Some of the hardware used includes lights, image acquisition equipment, and dark boxes. The software used includes image acquisition and preprocessing. To the best of our knowledge, this study is the first to propose this system and no similar study has been reported yet. Methods: We initially introduced the hardware and the software that we used in this study. The key technologies in this system, including lighting equipment, facial diagnosis device, facial information acquisition scales, image acquisition, and preprocessing were then introduced one by one. The hardware of this system consists of a light emitting diode (LED), a digital camera, a dark box, and a computer. Each of this hardware has its special function: the LED imitates natural light; the camera records facial images; the dark box imitates the consultation room; and the computer stores the images. The software is used to acquire and adjust the images. The image acquisition system uses the computer to control the opening and the closing of the camera, the photography, and the setup of relevant index to obtain the fully automatic photography of faces and information transmission. Results: The normalized acquisition system of facial diagnosis in TCM was tested according to the following procedures. (1) The lighting uniformity of acquisition windows was tested. Results of the uniformity test showed an even distribution of the illumination on the opening of the dark box for facial complexion collection. (2) TCM experts valuated the acquisition environment. The four features basically tallied among the different diagnoses of doctors, in which the lowest consistency was 75% for the lip color and the highest was 95% for the moist/dry lips. These data showed that the LED the natural light can be used efficiently in the image collecting process. (3) The correction of collected images was tested. The sum of the Euclidean distance between the uncorrected color and the standard color was 1296.345, whereas the sum between the corrected color and the standard color was 403.527. The maximum distances before and after correction were 163.68 and 44.69, respectively. The minimum distances before and after correction were 13.3 and 5.9, respectively. (4) We collected 4050 photos of patients using this system, which was proven to be stable. Conclusions: This article introduces an automated acquisition system of facial diagnosis in TCM. The safety of the system can be ensured. By comparing TCM under natural light and in the dark box, this system meets the requirements of clinical application in all of the collected samples (more than 4050). The acquisition system of facial diagnosis in TCM has also been applied efficiently in a few hospitals.

scholarly journals Analyzing the effect of moxibustion treatment using the maximum instantaneous frequency of photoplethysmogram signals based on Hilbert–Huang transforms

2021 ◽  
Vol 17 (1) ◽  
pp. 155014772199133
Author(s):  
Ming-Xia Xiao ◽  
Chang-Hua Lu ◽  
Na Ta ◽  
Hai-Cheng Wei ◽  
Xiao-Jing Tang
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Instantaneous Frequency ◽  
Cardiovascular Health ◽  
Pulse Signal ◽  
Maximum Energy ◽  
Average Weight ◽  
Before And After ◽  
Positive Effect ◽  
Acupoint Stimulation

Moxibustion is a treatment in traditional Chinese medicine that can promote human health. In order to study the effect of moxibustion on cardiovascular function, photoplethysmogram signals were obtained from acupoints at st 36 (zusanli), sp 6 (sanyinjiao), and li 11 (quchi) during moxibustion. The test data were collected from six volunteers of average weight for 6 days of treatment. The effect of moxibustion treatment was determined by comparing amplitude–frequency characteristics of the instantaneous frequency in the power spectrum after Hilbert–Huang transformation of the pulse signal before and after treatment. It was found that after moxibustion, the maximum energy frequency of the photoplethysmogram would increase, but the amplitude would decrease. The results show that moxibustion therapy has the dual benefit of the infrared effect and acupoint stimulation, which can improve cardiovascular health and has a positive effect on metabolism.

scholarly journals Effects of Traditional Chinese Medicine for Vaginal Lavage Combined with Psychological Intervention in Postoperative Patients with Cervical Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Rufen Ma ◽  
Ruixiang Yu ◽  
Yuchao Yuan ◽  
Baofeng Ren ◽  
Yuan Li
Keyword(s):  
Quality Of Life ◽  
Cervical Cancer ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Tumor Marker ◽  
T Lymphocyte ◽  
Psychological Intervention ◽  
Vaginal Lavage ◽  
Before And After

Purpose. To explore the effects of traditional Chinese medicine for vaginal lavage combined with psychological intervention on the immune function and clinical efficacy in patients with cervical cancer. Methods. Patients with cervical cancer treated in our hospital from January 2020 to May 2021 were included in this study. All patients were treated with traditional Chinese medicine for vaginal lavage combined with psychological nursing intervention. The treatment outcomes of the patients were observed, and the quality-of-life scores and depression of the patients before and after treatment were compared. Changes in T-lymphocyte subset-related indicators, changes in blood routine-related indicators, and changes in the detection level of tumor markers were compared with anxiety scores. Results. After treatment, depression and anxiety were significantly reduced and the patient’s quality of life significantly improved. After treatment, the patient’s CD3+, CD4+, and CD4+/CD8+ proportions were dramatically higher than before treatment ( P < 0.05 ), there was no significant difference in CD8+ proportion before and after treatment ( P > 0.05 ), and the white blood cell (WBC), hemoglobin (Hb), platelet (PLT) of patients, and the level of tumor marker (CA125) after treatment were immensely lower than before treatment ( P < 0.05 ). Conclusions. Treating patients with cervical cancer with traditional Chinese medicine for vaginal lavage combined with psychological nursing can effectively improve the patient’s immune function, effectively reduce the level of tumor marker CA125, increase the level of T-lymphocyte subsets, and improve the bone marrow hematopoietic function.

Traditional Chinese medicine (TCM) utilization among cancer survivors.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e20604-e20604
Author(s):  
Chun-Ru Chien ◽  
Ya-Chen T. Shih ◽  
Yu-Min Liao ◽  
Shan-Yu Su
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Cancer Survivors ◽  
Normal Population ◽  
Descriptive Analysis ◽  
Population Based ◽  
Control Group ◽  
Claim Database ◽  
Initiation Rate ◽  
Before And After

e20604 Background: We investigated the utilization patterns of traditional Chinese medicine (TCM) among general cancer survivors before and after their cancer diagnosis when compared to normal population. Methods: Using population-based claim database, we identified 87249 cancer survivors diagnosed within 2000-2004 and 87,237 matched controls. We obtained information on TCM utilization 2 years before and after their diagnosis. We quantified the utilization of TCM in two ways: initiation (any use in a specific year) and intensity (visit counts per year). Descriptive analysis was applied to explore the trends in initiation and intensity for all cancer and controls as well as each major cancer sites separately. Results: TCM intensity (mean annual visits, with 95%CI) was increased substantially (23%) for general cancer survivors after diagnosis: from 1.58 (1.55-1.61) for 1~2 years before diagnosis to 1.94 (1.9-1.98) for 2nd years after diagnosis, in contrast to the minor increase (10%) for the control group. For most of the major cancer sites, the initiation rate of TCM among cancer survivors increased before diagnosis then gradually returned to around baseline afterward, whereas the intensity increased gradually after diagnosis. The initiation and intensity for the control groups was relatively stable during the corresponding periods. Conclusions: We found that the initiation of TCM was increased in the pre-diagnostic period and the intensity of TCM gradually increased after diagnosis when compared to normal population. Further research is needed to explore whether TCM, or more broadly CAM, can either serve as a clinical alter or bring additional benefits to these patients.

scholarly journals Serum Metabolic Profiling Analysis of Gout Patients Treated with Traditional Chinese Medicine Tongfengtai Granules Based on Gas Chromatography-Mass Spectrometry

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Jianyong Zhang ◽  
Hong Pan ◽  
Jingjing Xie ◽  
Jing Wang ◽  
Ruyi Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Metabolic Profiling ◽  
Metabolic Pathways ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Before And After ◽  
Metabolic Biomarkers

Gout has become a public health problem that seriously threatens human health. Traditional Chinese medicines (TCMs) have a long history of treating gout and have some advantages compared with the conventional medicines. Compound TCM Tongfengtai granules are gradually being used for clinical treatment of gout, but its mechanism is still unclear. The purpose of this study was to explore the metabolic profiling of serum from gout patients before and after treatment with Tongfengtai granules and identify the differential metabolites and related metabolic pathways. A total of 40 gout patients hospitalized in Shenzhen Traditional Chinese Medicine Hospital from 2018 to March 2019 were recruited in the current study, and serum samples from these patients before and after treatment with Tongfengtai granules were collected. Gas chromatography-mass spectrometry (GC-MS) assay was used to identify serum metabolites. The OPLS-DA VIP method was used to screen for potential metabolic biomarkers, and MetaboAnalyst 4.0 was used to identify related metabolic pathways. The result showed that there was a significant difference in the concentrations of six metabolites in the serum after treatment: D-galactose, lactic acid, 3-hydroxybutyric acid, D-pyran (type) glucose, alanine, and L-isoleucine. Except D-pyran (type) glucose, the serum concentrations of the other five metabolites were all significantly reduced. Besides, pathway enrichment analysis found that these potential metabolic biomarkers were mainly involved in lactose degradation and the glucose-alanine cycle. Thus, the serum metabolic profiling of gout patients treated with Tongfengtai granules changed, and the differential metabolites and related metabolic pathways might provide clues for understanding the mechanism of Tongfengtai granules.

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