Diversity And Antibiograms of Secondary Bacterial Isolates From Cutaneous Leishmaniasis Wounds

Abstract Background: Leishmaniasis is a vector borne disease caused by an intracellular protozoan parasite. The presence of secondary bacterial infections in cutaneous leishmaniasis wounds exacerbate lesion development and could lead to delay in the healing process. Little is also known about the different bacteria species co-infecting leishmaniasis wounds and their sensitivity patterns in Ghana. This study sought to determine the resistance patterns of bacteria co-infecting cutaneous leishmaniasis wounds from selected communities in the Nkwanta district.Methods: Various bacteria were isolated and characterized from exudates obtained from wound swabs collected with sterile cotton tipped applicators. Confirmation of bacterial identity was done using the analytical profile index and the matrix-assisted laser desorption/ionization time of flight mass spectrometry. Antibiotic susceptibility tests were performed using agar disc diffusion method according to the Clinical and Laboratory Standards Institute breakpoint values.Results: A total of 42 secondary bacteria were isolated from the wounds among which S. aureus was the most predominant (31%). Other pathogenic bacteria that colonized the wounds included Bacillus subtilis (23.8%), Pantoea spp(11.9%), Klebsiella pneumoniea (7.1%), Enterobacter cloacae (7.1%), Aeromonas spp (4.8%), Serratia marcescens (4.8%),Serratia liquefacien (2.4%), Serratia plymutheca (2.4%), Providencia rettgeri (2.4%) and Cronobacter spp (2.4%). Majority of the isolates were obtained from Agoufie (21.4%), Baasare (19%), and Gekrong (16.7%). Most of the isolates were resistant to beta-lactam antibiotics and the third generation cephalosporin. Notably, 84.6% of the S. aureus isolates were methicillin and ciprofloxacin resistant whilst 92.3% were resistant to ampicllin. About sixty-nine percent (69.2%) showed intermediate susceptibility to Erythromycin. Additionally, S. plymutheca was resistant to all the test antibiotics. All the K. pneumoniae and E. cloacae isolates showed resistance to ampicillin, cefotaxime, ceftriaxone, ciprofloxacin, amikacin, aztreonam and meropenem but only 66.7% of these isolates were resistant to piperacillin. All isolates of Providencia rettgeri, Cronobacter spp, S. marcescen, S. liquefacien were resistant to all the beta-lactam antibiotics.Conclusion: This study suggests colonization of cutaneous leishmaniasis wounds with varied bacterial species that are mostly resistant to beta-lactam group of antibiotics.

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Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v1i3.26462 ◽

2016 ◽

Vol 1 (3) ◽

pp. 457-462 ◽

Cited By ~ 4

Author(s):

Md Nuruzzaman Munsi ◽

Nathu Ram Sarker ◽

Razia Khatun ◽

Mohammed Khorshed Alam

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pathogenic Bacteria ◽

Bacterial Species ◽

Salmonella Typhi ◽

Diffusion Method ◽

Biochemical Tests ◽

Public Health Importance ◽

Milk Samples ◽

First Choice

Cows milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Grams staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

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Growing Menace of Antibacterial Resistance in Clinical Isolates ofPseudomonas aeruginosain Nepal: An Insight of Beta-Lactamase Production

BioMed Research International ◽

10.1155/2016/6437208 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Shamshul Ansari ◽

Rabindra Dhital ◽

Sony Shrestha ◽

Sangita Thapa ◽

Ram Puri ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Antibiotic Resistance ◽

Opportunistic Pathogen ◽

Resistance Rate ◽

Diffusion Method ◽

Beta Lactamase ◽

Beta Lactam ◽

Beta Lactam Antibiotics ◽

Microbiological Methods ◽

Immunosuppressed Patients

Introduction. Pseudomonas aeruginosais the most frequently isolated organism as it acts as the opportunistic pathogen and can cause infections in immunosuppressed patients. The production of different types of beta-lactamases renders this organism resistant to many commonly used antimicrobials. Therefore, the aim of this study was to document the antibiotic resistance rate inPseudomonas aeruginosaisolated from different clinical specimens.Methods. Pseudomonas aeruginosarecovered was identified by standard microbiological methods. Antibiotic susceptibility testing was performed by modified Kirby-Bauer disc diffusion method following Clinical and Laboratory Standard Institute (CLSI) guidelines and all the suspected isolates were tested for the production of ESBLs, MBLs, and AmpC.Results.Out of total (178) isolates, 83.1% were recovered from the inpatient department (IPD). Majority of the isolates mediated resistance towards the beta-lactam antibiotics, while nearly half of the isolates were resistant to ciprofloxacin. Most of the aminoglycosides used showed resistance rate up to 75% but amikacin proved to be better option. No resistance to polymyxin was observed. ESBLs, MBLs, and AmpC mediated resistance was seen in 33.1%, 30.9%, and 15.7% isolates, respectively.Conclusions. Antibiotic resistance rate and beta-lactamase mediated resistance were high. Thus, regular surveillance of drug resistance is of utmost importance.

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INVESTIGATION OF THE SYNERGISTIC ANTIBACTERIAL ACTION OF COPPER NANOPARTICLES ON CERTAIN ANTIBIOTICS AGAINST HUMAN PATHOGENS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i10.28069 ◽

2018 ◽

Vol 10 (10) ◽

pp. 83

Author(s):

Selvarani Murugan

Keyword(s):

Antibacterial Activity ◽

Copper Nanoparticles ◽

Pathogenic Bacteria ◽

Color Change ◽

Quantitative Information ◽

Inorganic Nanoparticles ◽

Diffusion Method ◽

Human Pathogens ◽

Bacterial Strains ◽

Agar Disc

Objective: Resistance to antibacterial agents by pathogenic bacteria has emerged in recent years and is a major challenge for the healthcare industry. Copper nanoparticles (CuNPs) are known to be one of the multifunctional inorganic nanoparticles with effective antibacterial activity. Hence the present investigation has been focused on synthesizing and evaluating the bactericidal effect of copper nanoparticles.Methods: CuNPs were synthesized by reducing the aqueous solution of copper sulfate with sodium borohydride. The synthesized particles were characterized by x-ray diffractogram (XRD), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) techniques to analyze size, morphology and quantitative information respectively. The antibacterial activity of CuNPs was examined by agar well diffusion method. Synergistic effect of CuNPs with broad-spectrum antibiotics was determined by the agar disc diffusion method.Results: Color change of reaction mixture from blue to dark brown indicated the formation of CuNPs. SEM image clearly demonstrated that the synthesized particles were spherical in shape and its size was found to be 17.85 nm. EDS report confirmed the presence of elemental copper in the resultant nanoparticles and its accounts for major proportion (96%) of the mass of nanoparticles. Bacterial effect of CuNPs revealed that Pseudomonas aeruginosa showed the highest antibacterial sensitivity (16.00±1.63 mm), whereas least susceptibility (9.67±0.47 mm) was noticed against Staphylococcus aureus. An enhanced antibacterial activity of commercial antibiotics was also noticed when it combined with CuNPS. A minimum zone of inhibition was increased from 0.67±0.47 mm to 10.66±0.24 mm when the nanoparticles and antibiotics were given together.Conclusion: It was observed that copper nanoparticles exhibited profound activity against all the tested bacterial strains which shows that CuNPs may serve as a better option for use in medicine in the future.

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Species, Risk Factors, and Antimicrobial Susceptibility Profiles of Bacterial Isolates from HIV-Infected Patients Suspected to Have Pneumonia in Mekelle Zone, Tigray, Northern Ethiopia

BioMed Research International ◽

10.1155/2019/8768439 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 2

Author(s):

Gebre Adhanom ◽

Dawit Gebreegziabiher ◽

Yemane Weldu ◽

Araya Gebreyesus Wasihun ◽

Tadele Araya ◽

...

Keyword(s):

Risk Factors ◽

Antimicrobial Susceptibility ◽

Bacterial Pneumonia ◽

Bacterial Infections ◽

Bacterial Species ◽

Diffusion Method ◽

Resistant Bacteria ◽

P Value ◽

Northern Ethiopia ◽

Hiv Patients

Background. Pneumonia is a condition, where bacterial infections are implicated as the most common causes of morbidity and mortality in humans. The actual burden of HIV-infected patients with pneumonia is not well documented in Mekelle region of Ethiopia. This study estimated the prevalence of bacterial pneumonia in HIV patients, antimicrobial susceptibility patterns of pathogens implicated in pneumonia, and associated risk factors in Mekelle zone, Tigray, Northern Ethiopia, during August-December 2016. Methods. Sputum specimens were collected from 252 HIV seropositive individuals with suspected pneumonia. Data on sociodemographics and risk factors were also collected using a structured questionnaire. Blood, Chocolate, and Mac Conkey agar plates (Oxoid, Hampshire, UK) were used to grow the isolates. The isolated colonies were identified based on Gram stain, colony morphology, pigmentation, hemolysis, and biochemical tests. The antimicrobial susceptibility test was performed using the modified Kirby-Bauer disc diffusion method. The analysis was performed using SPSS version 22 and p-value < 0.05 with corresponding 95% confidence interval (CI) was considered statistically significant. Results. Out of the 252 samples, 110 (43.7%) were positive for various bacterial species. The predominant bacterial species were Klebsiella pneumoniae (n=26, 23.6 %) followed by Streptococcus pneumoniae (n=17, 15.5 %), Escherichia coli (n=16, 14.5%), Klebsiella spp. (n=15, 13.6%), Staphylococcus aureus (n=9, 8.2%), Enterobacter spp. (n=7, 6.3%), Pseudomonas aeruginosa (4, n=3.6%), Proteus spp. (n=4, 3.6%), Citrobacter freundii (n=7, 6.3%), Streptococcus pyogenes (3, 2.7%), and Haemophilus influenzae (n=2, 1.8%). Young age (18-29), recent CD4+ count less than 350 cells/mL, alcohol consumption, and HIV WHO stage II showed significant association with the occurrence of bacterial pneumonia. Resistance to penicillin, co-trimoxazole, and tetracycline was observed in 81.8%, 39.8%, and 24.5% of the isolates, respectively. Conclusions. The problem of pneumonia among HIV patients was significant in the study area. The high prevalence of drug-resistant bacteria isolated from the patient’s samples possesses a health risk in immunocompromised HIV patients. There is a need to strengthen and expand culture and susceptibility procedures for the administration of appropriate therapy to improve patients management and care which may aid in decreasing the mortality.

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Phenotypic detection of Carbapenamase among Klebsiella pneumoniae isolated from clinical samples using modified Hodged test

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2020.13.3.0326 ◽

2020 ◽

Vol 13 (3) ◽

pp. 135-140

Author(s):

HauwaYakubu ◽

Mahmud Yerima Iliyasu ◽

Asma’u Salisu ◽

Abdulmumin Ibrahim Sulaiman ◽

Fatima Tahir ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Susceptibility Testing ◽

University Teaching ◽

Diffusion Method ◽

Clinical Samples ◽

Beta Lactam ◽

Beta Lactamases ◽

Beta Lactam Antibiotics ◽

Microbiological Techniques ◽

Tract Infections

Carbapenemases are microbial enzymes that confer resistance to virtually all available beta-lactam antibiotics and the most frequent carbapenemases are the Klebsiella pneumoniae Carbapenamase (KPC). Detection of carbapenemases is a significant infection control strategy as the enzymes are often associated with extensive antimicrobial resistance, therapeutic failures and mortality associated with infectious diseases. A total of 400 clinical samples were collected from different groups of patients in Abubakar Tafawa Balewa University Teaching Hospital, Bauchi, Nigeria and 118 K. pneumoniae were isolated using standard microbiological techniques. The isolates were subjected to antibiotic susceptibility testing by Kirby-Bauer disc diffusion method, then screened for Carbapenamase production using modified Hodge test. The results indicated that the isolates were resistant to Ampicillin (61.9%), Ceftriaxone (50.8%) and Ceftazidime (50.8%), then Ciprofloxacin (54.2%), but predominantly sensitive to Imipenem (66.9%), Eterpenem (60.2%) and Meropenem (65.3%). It was found that 38 (32.2%) of the isolates phenotypically shows the presence of Carbapenamase, with highest frequency of (40.7%) among patients, mainly adult females with cases of Urinary Tract Infections (UTIs) and the least from wound (11.8%).This study revealed that the isolates produced other beta-lactamases than KPC or variants of Carbapenamase that cannot be detected by modified Hodge test, thus shows low resistance to carbapenems. Therefore further studies is needed to genotypically confirm the presence of KPC in these isolates.

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Cross-Reactivity of Drug-Dependent Antibodies in Patients with Immune Thrombocytopenia Induced By Beta-Lactam Antibiotics

Blood ◽

10.1182/blood-2019-131375 ◽

2019 ◽

Vol 134 (Supplement_1) ◽

pp. 2350-2350

Author(s):

Matthew John Slaught ◽

Daniel W. Bougie ◽

Richard H. Aster

Keyword(s):

Bacterial Infections ◽

Immune Thrombocytopenia ◽

Conflicts Of Interest ◽

Cross Reactivity ◽

Beta Lactam ◽

Cross Reactions ◽

Beta Lactam Antibiotics ◽

Wide Range ◽

Group 2 ◽

Group 1

More than 50 beta lactam (BL) antibiotics are now in active use for treatment of a wide range of bacterial infections. BL antibiotics are among the most common drugs capable of inducing antibodies (DDAbs) that cause drug-induced immune thrombocytopenia (DITP). Most DDAbs are highly specific for the sensitizing drug but beta lactams all have a common core structure and many similarities among side groups that are added to augment potency and modify specificity, raising the possibility that a DDAb specific for one BL may cross-react with another. We studied DDAbs from 33 patients with DITP induced by 9 commonly used BL drugs to determine whether patterns of cross-reactivity exist that might influence the choice of an alternative antibiotic in a patient with BL-induced DITP. DDAbs were demonstrated in a flow cytometric assay considered to be "positive" when immunoglobulins in patient serum but not normal serum react with normal platelets in the presence, but not in the absence of drug (Blood 2018;131:1486). DDAbs detected in the 33 patients were specific for 9 different BL drugs that were divided into two groups, "penicillins" (Group 1) and cephalosporins (Group 2) on the basis of structural similarities (Figure 1). In Group 1 were 19 DDAbs specific for amoxicillin (2), nafcillin (4) and piperacillin (13). Structurally similar ampicillin and penicillin were also tested with these abs. In Group 2 were 14 DDAbs specific for cefadroxil (1), cefepime (2), ceftazidime (2), ceftizoxime (1), ceftriaxone (7) and cephalexin 1). Cross-reactions identified within these groups of DDAbs are shown in Tables 1 and 2. Cross-reactions, many quite strong (S) were observed among DDAbs specific for drugs in both structural groups (Tables 1 and 2). Particularly noteworthy were cross-reactions of the 19 Group 1 DDAbs with ampicillin (6) and penicillin (6) (Table 1) and of the 14 Group 2 DDAbs with cefepime (6), ceftizoxazole (6) and ceftriaxone (3) (Table 2). The findings show that platelet-specific DDAbs induced by beta lactam antibiotics, in contrast with those induced by medications like quinine, sulfamethoxazole and vancomycin, commonly cross-react with other antibiotics of this class. In patients with immune thrombocytopenia induced by a beta lactam antibiotic, it may be prudent to avoid switching to another beta lactam or, if this is necessary, to monitor platelet counts carefully. Disclosures No relevant conflicts of interest to declare.

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Antibacterial Effect of Vernonia anthelmentica (Kattu Seeragam) on Bovine Mastitis

Vol. 5, No. 1 - International Journal of Zoological Investigations ◽

10.33745/ijzi.2021.v07i02.003 ◽

2021 ◽

Vol 7 (2) ◽

Author(s):

Sandhiya G ◽

Ahmed Fauzia

Keyword(s):

Pathogenic Bacteria ◽

Antibacterial Effect ◽

Bacterial Species ◽

Bovine Mastitis ◽

Seed Extract ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Common Disease ◽

Phytochemical Analysis ◽

Quality Of Milk

Bovine mastitis is the common disease of mammary glands of dairy cows, caused by pathogenic bacteria, such as Staphylococcus aureus, Enterococccus faecium, Escherichia coli, Klebsiella pneumonia, Bacillus etc. The disease severely impacts the quality of milk and associated milk products. Three different bacterial species Staphylococcus, Bacillus and Klebsiella were isolated, identified and later treated with the seed extract of Vernonia anthelmentica. Well diffusion method was used to test the antibacterial effect of ethanol seed extract of Vernonia anthelmentica. Results showed that Vernonia anthelmentica was most effective against Staphylococcus compared to other two bacterial species. Phytochemical analysis of Vernonia anthelmentica revealed the presence of tannins, saponins, terpenoids etc. Present study suggests that seeds of Vernonia anthelmentica can be an effective antibacterial against bovine mastitis, which might be due to thepresence of tannins, and terpenoids in the extract.

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Synthesis of a New Conjugates of Imidazole wih Beta Lactam Moiety and Evaluation of its Expanded Antibacterial Activity

Oriental Journal Of Chemistry ◽

10.13005/ojc/340535 ◽

2018 ◽

Vol 34 (5) ◽

pp. 2495-2501 ◽

Cited By ~ 1

Author(s):

Sarmd D. Noori ◽

Mazin N. Mousa ◽

Shaker A. N. Al-Jadaan

Keyword(s):

Antibacterial Activity ◽

Chemical Structure ◽

Bacterial Species ◽

Anaerobic Bacteria ◽

Diffusion Method ◽

Disc Diffusion ◽

Beta Lactam ◽

Disc Diffusion Method ◽

E Coli ◽

Ft Ir

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.

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Cockroaches as a Source of High Bacterial Pathogens with Multidrug Resistant Strains in Gondar Town, Ethiopia

BioMed Research International ◽

10.1155/2016/2825056 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Feleke Moges ◽

Setegn Eshetie ◽

Mengistu Endris ◽

Kahsay Huruy ◽

Dagnachew Muluye ◽

...

Keyword(s):

Bacterial Infections ◽

Bacterial Species ◽

Multidrug Resistant ◽

Resistance Rate ◽

Control Measures ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Gondar Town ◽

Resistant Strains ◽

And Control

Background. Cockroaches are source of bacterial infections and this study was aimed to assess bacterial isolates and their antimicrobial profiles from cockroaches in Gondar town, Ethiopia.Methods. A total of 60 cockroaches were collected from March 1 to May 30, 2014, in Gondar town. Bacterial species were isolated from external and internal parts of cockroaches. Disk diffusion method was used to determine antibiotic susceptibility patterns. Data were entered and analyzed by using SPSS version 20;Pvalues <0.005 were considered as statistically significant.Results. Of 181 identified bacteria species, 110 (60.8%) and 71 (39.2%) were identified from external and internal parts of cockroaches, respectively.Klebsiella pneumoniae32 (17.7%),Escherichia coli29 (16%), andCitrobacterspp. 27 (15%) were the predominant isolates. High resistance rate was observed to cotrimoxazole, 60 (33.1%), and least resistance rate was noted to ciprofloxacin, 2 (1.1%). Additionally, 116 (64.1%) of the isolates were MDR strains;Salmonellaspp. were the leading MDR isolates (100%) followed byEnterobacter(90.5%) andShigellaspp. (76.9%).Conclusion. Cockroaches are the potential source of bacteria pathogens with multidrug resistant strains and hence effective preventive and control measures are required to minimize cockroach related infections.

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COMPARATIVE ANALYSIS OF METAL NANOPARTICLES SYNTHESIZED FROM HIBISCUS ROSA SINESIS AND THEIR ANTIBACTERIAL ACTIVITY ESTIMATION AGAINST NINE PATHOGENIC BACTERIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i5.17458 ◽

2017 ◽

Vol 10 (5) ◽

pp. 323

Author(s):

Shruti Tyagi

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Au Nanoparticles ◽

Size Range ◽

Micrococcus Luteus ◽

Bacterial Species ◽

Cost Effective ◽

Antibacterial Activities ◽

Agar Disc ◽

Vis Spectroscopy

Objective: This study demonstates a simple, cost effective protocol for biosynthesis of stable silver (Ag) and gold (Au) nanoparticles from Hibiscus Rosa sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: Silver (Ag) and gold (Au) nanoparticles were biosynthesized from Hibiscus Rosa sinesis were characterized by UV–VIS spectroscopy, FTIR and TEM. The antibacterial activities of AgNPs and AuNPs were evaluated against 9 pathogenic bacterial species Pseudomonas aeroginosa, Bacillus subtilis Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogens, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Synthesized AgNPs were obtained in 13.01 to 28.14 nm size range, while AuNPs were in 6.32 to 18.19 nm size range. The results of Fourier transform infrared spectroscopy (FTIR) spectra indicates that the AuNPs are bound to amine groups and the AgNPs to carboxylate ion groups. The antibacterial activities of AgNPs, the zone of inhibition significantly increased with the increases of concentrations of AgNPs in all pathogenic bacterial species except in the case of S. epidermidis at 50%, S. aerogenes and A. hydrophila at 70%, while in case of AuNPs antibacterial activity was displayed only against B. subtilis at 20% and 100% concentration.Conclusion: This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from Hibiscus Rosa sinensis leaf extract and insights to their potential applicability as an alternative antibacterial agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria. Keywords: silver nanoparticles; gold nanoparticles; UV–VIS spectroscopy; FTIR; TEM; antibacterial activities.

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