scholarly journals Fractional CO2 laser combined with 595-nm PDL inhibiting hypertrophic scars via measuring BMP-7 and Fas expression in rabbit models

2020 ◽  
Author(s):  
Jinxia Zhang ◽  
Shuanglin Zhou ◽  
Zhikuan Xia ◽  
Zhuoying Peng ◽  
Rongya Yang
Keyword(s):  
Treatment Group ◽  
Therapeutic Strategy ◽  
Molecular Mechanisms ◽  
Therapy Group ◽  
Combined Treatment ◽  
Control Group ◽  
Rt Pcr ◽  
Combined Use ◽  
New Zealand White Rabbits ◽  
Immunohistochemical Studies

Abstract Background and ObjectivesAlthough the treatment of hypertrophic scar (HS) remain challenging, fractional CO2 laser (FCO2L) and 595-nm pulsed-dye laser (PDL) have proved clinical efficacy. Meanwhile, BMP-7 and Fas proteins are demonstrated to promote wound healing and inhibit scar formation, yet few reports on the effect of the two proteins on hypertrophic scarring are available, and their molecular mechanisms remain unclear. In current study, we attempted to observe the effect of combined use of FCO2L with 595-nm PDL in HS animal models through determining the expression of BMP-7 and Fas in scar inhibition.Materials and MethodsTwenty New Zealand white rabbits were randomized to control group, FCO2L group, PDL group and combined treatment group. Four HS samples were developed at each ear of individual rabbit. FCO2L was respectively applied to simple FCO2L and combined treatment group, and simple 595-nm PDL and combined treatment group. Totally, 3 sessions of treatments were carried out once every 14 days. Then, the changes of fibroblasts and collagens in HSs and expression of BMP-7 and Fas proteins in the scar tissues were determined via histological and immunohistochemical studies, ELISA, CCK8 test, RT-PCR and Western blot assay.ResultsHSs were flattened and shrunk after treatment, especially in rabbits treated by FCO2L plus 595-nm PDL group, in which obviously decreased abnormal fibroblast and collagen were noted. The deference was significant compared to other groups(P < 0.001). Moreover, expression of BMP-7 and Fas was both increased in the combined treatment group compared to single FCO2L or 595-nm PDL therapy group (P < 0.001) .ConclusionsFCO2L combined with 595-nm PDL can improve HSs in rabbit models by inhibiting excessive fibroblast growth and collagen deposition. This may be associated with increased BMP-7 and Fas expression in the scar tissues. And our findings may pioneer a new therapeutic strategy for alternative treatment of HSs.

scholarly journals Toxicological Effects of Silver Nanoparticles on Nile Tilapia (Oreochromis niloticus)

2020 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
Farahnaz Kakavand ◽  
Aliakbar Hedayati ◽  
Ali Jafar Nodeh ◽  
Saeid Maddah ◽  
Maryam Rezaei Shadegan
Keyword(s):  
Silver Nanoparticles ◽  
Treatment Group ◽  
Oreochromis Niloticus ◽  
Pleurotus Ostreatus ◽  
Combined Treatment ◽  
Control Group ◽  
Dissolved Metals ◽  
Toxicological Effects ◽  
Combined Use ◽  
Nile Tilapia Oreochromis Niloticus

The wide use of nano-sized metallic materials could result in the release of these particles into the environment. In addition, due to the dissolution of these nano-materials, some of the adverse effects could result from the dissolved metals. On the other hand, dietary supplements play a key role in improving the immunity of consumers; hence, the use of immune stimulants such as mushroom seems to be very necessary. In this study, the dietary effect of Pleurotus ostreatus mushroom was investigated on serum immunity and liver histopathological indices of Oreochromis niloticus exposed to silver nanoparticles (AgNPs). Serum total protein level enhanced with increasing prebiotic concentration. Serum immunoglobulin and albumin levels increased in the group treated with Pleurotus ostreatus. The amount of these indices significantly increased at the concentration of 0.2 mg/kg; however, glucose value decreased in both of the experimental groups. Serum ALT and ALP levels significantly reduced in the combined treatment group (AgNPs and mushroom) at the concentration of 0.2 mg/kg, in contrast with the control group. In the combination treatment group (AgNPs and mushroom), the serum AST level significantly reduced at concentrations of 0.1 mg/kg and 0.2 mg/kg; however, it increased at the concentration of 0.05 mg/kg compared with the control group, indicating that immunological indices could improve due to the combined use of AgNPs and mushroom. The overall conclusion of this study shows that the use of mushroom at 0.2 mg/kg in combination with AgNPs could partially improve the effect of AgNPs on tilapia.

scholarly journals The potential renal toxicity of silver nanoparticles after repeated oral exposure and its underlying mechanisms

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hamed Nosrati ◽  
Manijeh Hamzepoor ◽  
Maryam Sohrabi ◽  
Massoud Saidijam ◽  
Mohammad Javad Assari ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Molecular Mechanisms ◽  
Renal Toxicity ◽  
Periodic Acid ◽  
Critical Role ◽  
Oral Exposure ◽  
Control Group ◽  
Rt Pcr ◽  
Periodic Acid Schiff

Abstract Background Silver nanoparticles (AgNPs) can accumulate in various organs after oral exposure. The main objective of the current study is to evaluate the renal toxicity induced by AgNPs after repeated oral exposure and to determine the relevant molecular mechanisms. Methods In this study, 40 male Wistar rats were treated with solutions containing 30, 125, 300, and 700 mg/kg of AgNPs. After 28 days of exposure, histopathological changes were assessed using hematoxylin-eosin (H&E), Masson’s trichrome, and periodic acid-Schiff (PAS) staining. Apoptosis was quantified by TUNEL and immunohistochemistry of caspase-3, and the level of expression of the mRNAs of growth factors was determined using RT-PCR. Results Histopathologic examination revealed degenerative changes in the glomeruli, loss of tubular architecture, loss of brush border, and interrupted tubular basal laminae. These changes were more noticeable in groups treated with 30 and 125 mg/kg. The collagen intensity increased in the group treated with 30 mg/kg in both the cortex and the medulla. Apoptosis was much more evident in middle-dose groups (i.e., 125 and 300 mg/kg). The results of RT-PCR indicated that Bcl-2 and Bax mRNAs upregulated in the treated groups (p < 0.05). Moreover, the data related to EGF, TNF-α, and TGF-β1 revealed that AgNPs induced significant changes in gene expression in the groups treated with 30 and 700 mg/kg compared to the control group. Conclusion Our observations showed that AgNPs played a critical role in in vivo renal toxicity.

scholarly journals Diagnostic significance of Lactobacillus spp. identification in ejaculate

2020 ◽  
pp. 38-44
Author(s):  
DG Pochernikov ◽  
NT Postovoytenko ◽  
VV Getman ◽  
IS Galkina
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Polymerase Chain Reaction Method ◽  
Sex Hormone Binding Globulin ◽  
Control Group ◽  
Rt Pcr ◽  
Diagnostic Significance ◽  
Component Structure ◽  
Hormonal Imbalance ◽  
Lactobacillus Spp

Popularization of the real-time polymerase chain reaction method (RT-PCR), which is a trend of the recent years, allowed to significantly expand of the range of microorganisms that can be detected in the genitourinary tract of men. Moreover, the available picture of the microbiome's bacterial component structure became more detailed. Lactobacillus spp. remains one of the least studied groups of microorganisms. Treating patients with reproductive disorders, the authors have accumulated clinical experience demonstrating the possible relationship between presence of Lactobacillus spp. in the ejaculate and changes in the level of sex hormones and the key values registered with a spermogram. This study aimed to compare the levels of luteinizing hormone, follicle-stimulating hormone, testosterone, estradiol, prolactin, progesterone, and sex hormone binding globulin (SHBG) in blood serum and changes in spermogram values in 210 men with and without Lactobacillus spp. detected in their ejaculate. The treatment group included 105 men whose ejaculate had Lactobacillus spp. in the amount of (Lg) ≥ 103, as detected by RT-PCR. The control group included 105 men whose ejaculate did not have Lactobacillus spp. detected; the microbiome's bacterial component structure of their ejaculate was normal. Compared to the control group, treatment group had hormonal disorders registered more often: abnormal levels of three or more hormones (p = 0.04), hyperestradiolemia (p = 0.05), increased level of SHBG (p = 0.01). It was established that the presence of Lactobacillus spp. in the ejaculate of treatment group participants is associated with oligoastenoteratozoospermia (p < 0.01), decreased concentration of spermatozoa (p = 0.01), their decreased motility (p < 0.01) morphology abnormalities (p < 0.01). Thus, the presence of Lactobacillus spp. in the ejaculate can be interpreted as an additional marker of hormonal imbalance and fertility dysfunction in men.

scholarly journals An animal model study on the gene expression profile of meniscal degeneration

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yehan Fang ◽  
Hui Huang ◽  
Gang Zhou ◽  
Qinghua Wang ◽  
Feng Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pathway Analysis ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Control Group ◽  
Ion Binding ◽  
Endoplasmic Reticulum Membrane ◽  
Rt Pcr ◽  
Go Analysis ◽  
Meniscal Degeneration

AbstractMeniscal degeneration is a very common condition in elderly individuals, but the underlying mechanisms of its occurrence are not completely clear. This study examines the molecular mechanisms of meniscal degeneration. The anterior cruciate ligament (ACL) and lateral collateral ligament (LCL) of the right rear limbs of seven Wuzhishan mini-pigs were resected (meniscal degeneration group), and the left rear legs were sham-operated (control group). After 6 months, samples were taken for gene chip analysis, including differentially expressed gene (DEG) analysis, gene ontology (GO) analysis, clustering analysis, and pathway analysis. The selected 12 DEGs were validated by real time reverse transcription-polymerase chain reaction (RT-PCR). The two groups showed specific and highly clustered DEGs. A total of 893 DEGs were found, in which 537 are upregulated, and 356 are downregulated. The GO analysis showed that the significantly affected biological processes include nitric oxide metabolic process, male sex differentiation, and mesenchymal morphogenesis, the significantly affected cellular components include the endoplasmic reticulum membrane, and the significantly affected molecular functions include transition metal ion binding and iron ion binding. The pathway analysis showed that the significantly affected pathways include type II diabetes mellitus, inflammatory mediator regulation of TRP channels, and AMPK signaling pathway. The results of RT-PCR indicate that the microarray data accurately reflects the gene expression patterns. These findings indicate that several molecular mechanisms are involved in the development of meniscal degeneration, thus improving our understanding of meniscal degeneration and provide molecular therapeutic targets in the future.

Labor Pain Management: Effect of Pelvic Tilt by Birth Ball, Sacrum- Perinea Heat Therapy, and Combined Use of them, a Randomized Controlled Trial

2016 ◽  
Vol 33 (S1) ◽  
pp. S503-S503 ◽  
Author(s):  
S. Taavoni ◽  
S. Abdolahian ◽  
L. Neisani ◽  
H. Hamid
Keyword(s):  
Pain Management ◽  
Pelvic Tilt ◽  
Therapy Group ◽  
Controlled Trial ◽  
Personal Characteristic ◽  
Active Phase ◽  
Labor Pain ◽  
Control Group ◽  
Combined Use ◽  
Heat Therapy

There are various safe non-pharmacologic methods for labor pain management, which mostly decrees suffering of mother and some of them significantly decrease pain too.AimTo assess effect of pelvic tilt by birth ball, sacrum-perinea heat therapy and combination use of them on active phase of physiologic labor.MethodIn this randomized control trial, 120 primiparous volunteer with age 18-35 years, gestational age of 38–40 weeks, in one of hospitals of Iran university of medical sciences were randomly selected and divided in four groups: Pelvic tilt by using birth ball, sacrum perinea heat therapy, combined use of two mentioned methods and control group. Tools had 3 main parts of personal characteristic, client examination form and pain visual analogue scale (VAS). All ethical points were considered.ResultsEquality of four groups had been checked before intervention. Lowest pain score first belong to pelvic tilt by birth ball then combined group and finally in heat therapy, which all were significantly less than control group. Significant decrease of pain had been seen in birth ball group and combined group during after 30 minutes intervention, but in the heat therapy group, it was seen after 60 minutes intervention (P-value < 0.05).ConclusionAll three interventions of this study had significant effect and decreased labor pain during active phase, but highest decrease was in pelvic tilt by birth ball group and its effect started after 30 minutes intervention. It is suggested that that Obstetrics and Midwives consider these safe methods for labor pain management.Disclosure of interestThe authors have not supplied their declaration of competing interest.

scholarly journals Combined effect ofLactobacillus acidophilusandβ-cyclodextrin on serum cholesterol in pigs

2015 ◽  
Vol 115 (1) ◽  
pp. 1-5 ◽  
Author(s):  
L. Alonso ◽  
J. Fontecha ◽  
P. Cuesta
Keyword(s):  
Treatment Group ◽  
Serum Cholesterol ◽  
Ldl Cholesterol ◽  
Combined Treatment ◽  
Combined Effect ◽  
Total Serum ◽  
Control Group ◽  
Total Serum Cholesterol ◽  
Treatment Groups ◽  
The Individual

AbstractA total of twenty-four Yorkshire gilt pigs of 6–7 weeks of age were used in a 2×2 factorial experiment to determine the individual and combined effects of the inclusion of two dietary factors (cholesterol rich, 3 %β-cyclodextrin (BCD) andLactobacillus acidophiluscultures) on total cholesterol and LDL-cholesterol levels in blood serum. Pigs were assigned randomly to treatment groups (n6). Total serum cholesterol concentrations decreased after 3 weeks in all the experimental treatment groups, including diets with BCD,L.acidophilusor both. Similar trends were observed for serum LDL-cholesterol concentrations among the experimental treatments. No statistically significant differences from the control group were observed in either total serum cholesterol or LDL-cholesterol concentrations (P<0·05) for each of the individual treatment groups: BCD orL.acidophilus. However, significant differences in total serum cholesterol concentrations were observed when comparing the combined treatment group (BCD andL.acidophilus) with the control group, which consisted of a basal diet and sterile milk. The combined treatment group exhibited 17·9 % lower total serum cholesterol concentration after 3 weeks. Similar significant differences were observed when comparing the combined effect experimental group with the control group after 3 weeks. The combined treatment group exhibited 27·9 % lower serum LDL-cholesterol concentrations.

scholarly journals Application Effect Evaluation of Telmisartan combined with Spironolactone after Catheter Ablation of Patients with Paroxysmal Atrial Fibrillation

2021 ◽  
Vol 5 (3) ◽  
Author(s):  
Rongcheng Zhao ◽  
Jia Han ◽  
Lei Zhao
Keyword(s):  
Atrial Fibrillation ◽  
Treatment Group ◽  
Catheter Ablation ◽  
Paroxysmal Atrial Fibrillation ◽  
Conventional Therapy ◽  
Therapy Group ◽  
Combined Treatment ◽  
Time To Recurrence ◽  
Conventional Therapy Group ◽  
Hs Crp

Objective: To evaluate the application effect of telmisartan combined with spironolactone after catheter ablation of patients with paroxysmal atrial fibrillation. Methods: 80 cases of patients with paroxysmal atrial fibrillation who received radiofrequency catheter ablation treatment from March 2013 to March 2016 in our hospital were randomly selected, these patients were divided into two groups according to the treatment methods, namely, the telmisartan with Spironolactone treatment group (combined treatment group, n=40) and the conventional therapy group (n=40). The hs-CRP, NT-proBNP, LAD and recurrence of the two groups were analyzed. Results: The hs-CRP, NT-proBNP levels after 3 months of the combined treatment group were significantly lower (P<0.05), the recurrence rate 10.0% (4/40) was significantly lower than the conventional therapy group 27.5% (11/40) (P<0.05), the time to recurrence was significantly longer than the conventional therapy group (P<0.05). Conclusion: The application effects of telmisartan combined with spironolactone after catheter ablation in the treatment of patients with paroxysmal atrial fibrillation are better than conventional therapy.

scholarly journals Collaborative Influence of Elevated CO2 Concentration and High Temperature on Potato Biomass Accumulation and Characteristics

2019 ◽  
Vol 17 (1) ◽  
pp. 728-737
Author(s):  
Yao Yubi ◽  
Lei Jun ◽  
Niu Haiyang ◽  
Zhang Xiuyun
Keyword(s):  
High Temperature ◽  
Treatment Group ◽  
Aboveground Biomass ◽  
Combined Treatment ◽  
Biomass Accumulation ◽  
Tuber Formation ◽  
Control Group ◽  
Fresh Weight ◽  
Occurrence Time ◽  
Tuber Fresh Weight

AbstractAn experiment with OTC (Open-top Chamber) was conducted to study the influence of elevated CO2 concentration and high temperature on potato yields and quality, particularly the collaborative influence of these two factors on the accumulation of aboveground biomass (leaves, petiole, and stem), and underground biomass (root and tuber) in potato, and the influence on potato characteristics. The results showed that the accumulation of dry weight of potato stem and aboveground biomass under the combined treatment of elevated CO2 concentration and high temperature (warming) was significantly higher than that of the control group by 35.8%-53.4% and significantly higher than that of the warming treatment group by 24.4%34.4%. In terms of potato stem and aboveground biomass in the combined treatment group, the occurrence time of peak accumulation was postponed, but the accumulation velocity was increased; the interval days of fast biomass accumulation was prolonged than the warming treatment group and the control group. In this combined treatment experiments, the fresh weight accumulation of potato tuber was lower than the warming treatment group by 5% during the middle stage of tuber formation. But the fresh weight accumulation in the combined treatment was higher than the warming treatment group and the control group during the rest stages of tuber formation: the tuber fresh weight in the mature stage was higher than the warming treatment group and control group by 24.1%, and 3.4%, respectively. In terms of tuber fresh weight in the combined treatment group, the occurrence time of peak accumulation was postponed; the interval days of fast accumulation was prolonged compare to the warming treatment, but close to the control group.

Effects of low-dose ketanserin on atherosclerosis in rats and rabbits

2010 ◽  
Vol 88 (11) ◽  
pp. 1054-1060 ◽  
Author(s):  
Yong-Sheng Yu ◽  
He-Hui Xie ◽  
Ling Li ◽  
Shu-Wei Song ◽  
Ping Han ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vitamin D ◽  
New Zealand ◽  
Treatment Group ◽  
Low Dose ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
New Zealand White Rabbits

The present study was designed to test the hypothesis that a small dose of ketanserin, which enhances baroreflex activity, prevents the early lesions of atherosclerosis. In experiment 1, baroreflex sensitivity (BRS) was measured in 31 spontaneously hypertensive rats (SHRs) in a conscious state using a computerized blood pressure monitoring system. Four weeks later, the rats were administered vitamin D3 and fed a high-cholesterol diet for 8 weeks to induce atherosclerosis. Then their hearts and aortae were removed for pathological examination. A negative correlation was found between BRS and the scores of coronary (r = –0.460, P < 0.01) and aortic atherosclerosis (r = –0.448, P < 0.05) in SHR. In experiment 2, SHRs were divided into 3 groups (n = 10 in each group) and received a dose of ketanserin of 0.3, 1.0, and 3.0 mg/kg (i.g.), respectively. At the smallest dose (0.3 mg/kg), ketanserin did not lower blood pressure but enhanced BRS. In experiment 3, SHRs were administered vitamin D3, fed a high-cholesterol diet, and simultaneously treated with low-dose ketanserin. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (coronary score: 0.90 ± 0.14 vs. 1.76 ± 0.27, P < 0.05; aortic scores: 1.00 ± 0.39 vs. 2.18 ± 0.41, P < 0.05). In experiment 4, male New Zealand White rabbits were fed a high-cholesterol diet and treated with low-dose ketanserin at the same time. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (aortic scores: 0.26 ± 0.20 vs. 0.60 ± 0.31, P < 0.05). In conclusion, the present study demonstrated, for the first time, that low-dose ketanserin prevented the development of atherosclerosis independent of its blood pressure lowering action in SHRs and New Zealand White rabbits at least in part via enhancement of arterial baroreflex function.

scholarly journals COGNITIVE THERAPY GROUP DYNAMICS MODEL FOR REDUCING THE LEVEL OF DEPRESSED PATIENTS IN MENTAL HOSPITAL

2018 ◽  
Vol 1 (2) ◽  
pp. 131
Author(s):  
Arum Pratiwi
Keyword(s):  
Treatment Group ◽  
Cognitive Therapy ◽  
Group Dynamics ◽  
Therapy Group ◽  
Treated Group ◽  
Control Group ◽  
Mild Depression ◽  
Depressed Patients ◽  
Moderate Depression ◽  
Post Test

Introduction. The worst impact of depression is suicide, it need comprehensive management. Model of cognitive therapy through group dynamics try to break the chain, so, that the body does not respond to stimuli that lead to increased serotonin. The purpose of this study was create a new model of nursing care, describe the personal characteristics of depressed patients, describe of the level of depressed patients and compare depressed patients between the control group and the treatment group. Method. The design of this study was quasi experiments with pre test and post test control group. The sample study used purposive sampling strategies, that was patients in mental hospitals and selected with screening and than they were chosen with mild and moderate categories. The samples were 32 patients, 16 patients were a control group and 16 patients were a treated group. Patients were given a therapy model for one month which is 2 times a week. After that the level of depression were measured by BECK questionnaires. The data were analyzed by using independent t test. Result. The results showed that the distribution of the level of depression at pretest, 62.5% of mild depression in the treatment group and 62.5% of moderate depression in the control group. The result of post test, the mild depression decreased to 25%, and the moderate depression to 37.5%. The t test results concluded that the two groups there was difference in rates of depression between the control group and the treated group with t value significance 7.000 and 0.000. Discussion. In future studies it would be better if the treatment done in the longer term and depression levels were analyzed periodically. Keywords: Cognitive Therapy, Group Dynamics, Depressed Patients

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