scholarly journals Multi-Block Data Integration Analysis for Identifying and Validating Targeted N-Glycans as Biomarkers for Type II Diabetes Mellitus

2022 ◽  
Author(s):  
Eric Adua ◽  
Ebenezer Afrifa Yamoah ◽  
Emmanuel Peprah-Yamoah ◽  
Enoch Odame Anto ◽  
Emmanuel Acheampong ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
High Performance ◽  
Univariate Analysis ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Type Ii Diabetes Mellitus ◽  
Healthy Controls ◽  
Type Ii ◽  
Agglomerative Hierarchical Cluster Analysis

Abstract Plasma N-glycan profiles have been shown to be defective in type II diabetes Mellitus (T2DM) and holds a promise to discovering biomarkers. The study comprised 232 T2DM patients and 219 healthy individuals. N-glycans were analysed by high-performance liquid chromatography. Principal component analysis (PCA), discriminatory analysis and agglomerative hierarchical cluster analysis (HCA) were performed. N-glycan groups (GPs 34, 32, 26, 31, 36 and 30) were significantly expressed in T2DM in component 1 and GPs 38 and 20 were related to T2DM in component 2. Four clusters based on the correlation of the expressive signatures of the 39 N-glycans across T2DM and controls. Cluster A, B, C and D had 16, 16, 4 and 3 N-glycans respectively, of which 11, 8, 1 and 1 were found to express differently between controls and T2DM in a univariate analysis P. Multi-block analysis revealed that trigalactosylated (G3), triantennary (TRIA), high branching (HB) and trisialylated (S3) expressed significantly highly in T2DM than healthy controls. A bipartite relevance network revealed that HB, monogalactosylated (G1) and G3 were central in the network and observed more connections, highlighting their importance in discriminating between T2DM and healthy controls. Investigation of these N-glycans can enhance the understanding of T2DM.

scholarly journals Asymptomatic bacteriuria and its associated factors in type II diabetes mellitus

2017 ◽  
Vol 4 (6) ◽  
pp. 1633
Author(s):  
Dinesh Gurjar ◽  
Akash Mathur ◽  
Ramkrishna Sai ◽  
Arvind Lakesar ◽  
Puneet Saxena
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
Asymptomatic Bacteriuria ◽  
Type Ii Diabetes Mellitus ◽  
Control Group ◽  
Healthy Controls ◽  
Type Ii ◽  
E Coli ◽  
Diabetic Population ◽  
Culture Negative

Background: With the dawn of modern era the diabetes epidemic has spread over continents affecting the developed and the developing nations alike. Asymptomatic bacteriuria which is quite prevalent in the diabetic population is associated with increased diabetic complications and thereby increased morbidity. Thus, the aim of this study was to assess the proportion of cases with asymptomatic bacteriuria (ASB) among type II diabetes mellitus patients as compared to non-diabetic healthy controls and studying the factors associated with ASB.Methods: 93 eligible type II diabetes mellitus cases without genitourinary symptoms or abnormalities along with 93 non-diabetic healthy controls were recruited. Mid-stream urine was collected after taking informed consent and each sample tested using the dipstick, microscopy and culture techniques. Isolates were identified using standard biochemical tests.Results: Prevalence of ASB in our study was found to be 34.4% among cases of type II diabetes mellitus while it was 6.45% among non-diabetic healthy controls. Amongst the diabetics with ASB 71.9 % were females and 28.13 % were males. E. Coli was isolated in 21 subjects among diabetic cases (22.58%) and in 5 subjects among non-diabetic healthy controls (5.38%). Fasting blood sugar (FBS) in diabetic cases group was 151.08 ± 48.16 and in control group was found to be 98.57 ± 25.95 (p<0.001). Mean duration of patients with DM who had ASB (32) was 8.46 ± 4.14 years and those who were culture negative was 4.59 ± 4.2 years (p < 0.05). 24 subjects (75%) out of 32 diabetic ASB cases had glycosuria compared to 27 subjects (44.26%) out of 61 culture negative cases (p < 0.05). 9 out of 32 (28.13%) diabetic ASB subjects had proteinuria compared to 4 out of 61 (6.56%) culture negative cases (p < 0.05).Conclusions: Overall prevalence of ASB was significantly more in diabetic population as compared to non-diabetic healthy controls (34.4% vs 6.45%). Females have an increased likelihood of developing ASB as compared to males. E. Coli was the most common pathogen isolated in ASB cases. Longer duration of diabetes and poor glycemic control are independent predictors for the development of ASB. The risk of ASB is also significantly increased in those with glycosuria and proteinuria.

scholarly journals Pengaruh konseling menggunakan media booklet terhadap pengetahuan dan sikap pada pasien diabetes melitus

2021 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Riza Trisda ◽  
Saipul Bakri
Keyword(s):  
Diabetes Mellitus ◽  
Type Ii Diabetes ◽  
Univariate Analysis ◽  
Type Ii Diabetes Mellitus ◽  
Type Ii ◽  
Knowledge And Attitudes ◽  
Post Test ◽  
Sugar Levels ◽  
Bivariate Test ◽  
Diabetes Melitus

Background: Diabetes mellitus (DM) or diabetes is a health disorder in the form of a collection of symptoms that arise in a person caused by an increase in blood sugar levels due to insulin deficiency or insulin resistance and metabolic disorders in general.Objective: To determine the effect of counseling with booklet media on knowledge, attitudes in type II diabetes mellitus patients at Iskandar Muda Kindergarten II HospitalMethods: This study is a Quasi Experiment with one group pre-test post-test design using total sampling. This research was conducted at Kindergarten II Iskandar Muda Hospital Banda Aceh in 2018. The descriptive univariate analysis includes frequency distribution and percentage, bivariate test with dependent t-testResults: It shows that there is a significant effect of counseling using booklet media in increasing knowledge (p= 0.000) and attitudes (p= 0.001) of diabetes mellitus patients in Iskandar Muda Kindergarten II hospital.Conclusion: Counseling using booklet media can increase knowledge and attitudes of type II diabetes mellitus patients.

scholarly journals Prevalence of type II diabetes mellitus and its associated sociodemographic risk factors found in Nachanwel and Pishor revenue circle of Aurangabad district

2017 ◽  
Vol 4 (7) ◽  
pp. 2312
Author(s):  
Sameer S. Naval ◽  
Swati M. Mahajan ◽  
Vallabh B. Yadav
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Rural Area ◽  
Type Ii Diabetes ◽  
Univariate Analysis ◽  
Health Centre ◽  
Type Ii Diabetes Mellitus ◽  
Type Ii ◽  
Socioeconomic Class ◽  
Primary Health

Background: This particular study was conducted in rural area of deep interiors of Marathwada with the objective to find prevalence of type ii diabetes mellitus and to understand the interrelationship of diabetes mellitus and the other associated risk factors in the area with the particular focus on people staying in the area.Methods: This was a cross sectional study conducted in selected rural area of revenue circle Pishor and Nachanwel of selected Kannad tehesil covering 6 Villages with approximate households of 5000. The study was done in adults greater than 20 years of age. Only one member from each household was included for the study, using simple random sampling by lottery method from each of the family till we cover 20% of the households as a sample from each of the village to get a total of 1000 which is more than calculated sample size i.e. 900. Results: The prevalence of diabetes in the study area was 11.2% while that of pre diabetes was 6.6%. Prevalence in males was 12.8% while that in females was 9.02%. The risk Factors associated with diabetes in this study on univariate analysis were gender (male), age (increasing age), type of occupation, education, higher socioeconomic class, family H/O Diabetes, whereas on multivariate analysis age, occupation, socioeconomic class, were found to be significantly associated. Conclusions: Prevalence of 11.2% is large; it does give a warning signal of upcoming epidemic of diabetes all over India. High risk strategies should be adopted for screening of diabetes at the level of primary health centre to detect people with diabetes and impaired glucose tolerance at earliest possible time. ‘Primary Health Care’ approach with focus on the common risk factors is needed even in remote rural areas.

scholarly journals Recent trends in the antimicrobial susceptibility patterns of urinary pathogens in type II diabetes mellitus

2018 ◽  
Vol 6 (4) ◽  
pp. 1288
Author(s):  
Dinesh Gurjar ◽  
Akash Mathur ◽  
Ramkrishna Sai ◽  
Arvind Lakesar ◽  
Puneet Saxena
Keyword(s):  
Diabetes Mellitus ◽  
Antimicrobial Susceptibility ◽  
Type Ii Diabetes ◽  
Type Ii Diabetes Mellitus ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Type Ii ◽  
E Coli ◽  
Recent Trends ◽  
Susceptibility Patterns

Background: Diabetes mellitus is one of the most frequently encountered diseases in clinical practice and since the diabetic patients are at an increased risk of infections specially those of the urinary tract it is imperative for a physician to be aware of the prevalence and antibiotic susceptibility patterns of urinary pathogens. Thus, in this study we assess the recent trends in antimicrobial susceptibility patterns of urinary pathogens in type II diabetes mellitus.Methods: Ninety-three eligible type II diabetes mellitus cases without genitourinary symptoms or abnormalities along with 93 non-diabetic healthy controls were recruited. Mid-stream urine was collected after taking informed consent and each sample tested using the dipstick, microscopy and culture techniques. Isolates were identified using standard biochemical tests.Results: Prevalence of asymptomatic bacteriuria (ASB) in our study was found to be 34.4% among cases of type II diabetes mellitus while it was 6.45% among non-diabetic healthy controls. E. coli was the most common urinary pathogen isolated. E. coli susceptibility towards amikacin was 85.71%, towards ceftriaxone and nitrofurantoin was 71.73% and for meropenem and doxycycline 66.67% susceptibility was observed. In the one case where pseudomonas was cultured, it was susceptible to meropenem, gentamycin, cefoperazone-sulbactum and cefuroxime. In an isolated case where Proteus species was grown, it showed susceptibility to meropenem, norfloxacin, levofloxacin and co-trimoxazole. Enterobacter species which was grown, showed susceptibility to meropenem, vancomycin, amikacin, nitrofurantoin, norfloxacin, levofloxacin and co-trimoxazole. Gram positive bacteria mainly showed susceptibility to ceftriaxone, teicoplanin, vancomycin and doxycycline.Conclusions: The prevalence of bacteriuria is significantly higher in diabetics as compared to non-diabetics and with the recent trends suggestive of emerging resistance among urinary pathogens to some of the commonly used anti-microbials it is of utmost importance to carry out regular surveillance of bacterial profile and their anti-microbial susceptibilities to formulate updated guidelines for effectively treating urinary infections in diabetic patients.

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