scholarly journals Metagenomic and Metabolic Analyses of Poly-Extreme Microbiome from an Active Crater Volcano Lake

2021 ◽  
Author(s):  
Betsy Anaid Peña-Ocaña ◽  
Javier Tamames ◽  
Luis Eduardo Servín-Garcidueñas ◽  
Elena González-Toril ◽  
Cesar Iván Ovando-Ovando ◽  
...  
Keyword(s):  
Active Volcano ◽  
Microbial Consortia ◽  
Extreme Conditions ◽  
New Information ◽  
Active Crater ◽  
Close Relationship ◽  
Metabolic Interactions ◽  
Functional Features ◽  
Biotechnological Processes ◽  
Chichón Volcano

Abstract Background: El Chichón volcano is one of the most active volcanoes in Mexico. Previous studies have described the poly-extreme conditions of the lake crater but its bacterial composition and the functional features of the complete microbiome have not been characterized yet. Methods: This study integrated two approaches to explain the microbiology diversity and abundance, one focused on the environmental genomic potential by metagenomics approach, and other culturomics of enrichment of bacteria and archaea. The microbial diversity of the anaerobic consortia cultivated in was carried out by metabarcoding analysis, the metabolic capacity by metabolomics fluxes of carbon and enzymologic techniques for the analysis of sulfate reduction in laboratory-grown prokaryotic cells. Results: This work provides new information on the taxonomic and functional diversity of the Archea representative phyla Crenarchaeota and Euryarchaeota as well as the phyla Thermotogales and Aquificae for Bacteria. Through the analysis of microbial consortia cultivation and the genetic information collected from the natural environment sampling, metabolic interactions were identified between the microorganisms that support the life of the microbiome under multi-extreme conditions. A close relationship is proposed between the cycles of carbon and sulfur in an active volcano. Conclusions: This research contributes to the understanding of microbial metabolism under extreme conditions and potential knowledge of "microbial dark matter" that can be applied in biotechnological processes and evolutionary studies.

scholarly journals Carbohydrate complexity structures stable diversity in gut-derived microbial consortia under high dilution pressure

2020 ◽  
Author(s):  
Tianming Yao ◽  
Ming-Hsu Chen ◽  
Stephen R. Lindemann
Keyword(s):  
Microbial Diversity ◽  
Structural Complexity ◽  
Structure And Function ◽  
Microbial Consortia ◽  
Carbohydrate Structure ◽  
High Dilution ◽  
Chemical Complexity ◽  
Metabolic Interactions ◽  
And Function

ABSTRACTDietary fibers are major substrates for the colonic microbiota, but the structural specificity of these fibers for the diversity, structure, and function of gut microbial communities are poorly understood. Here, we employed an in vitro sequential batch fecal culture approach to determine: 1) whether the chemical complexity of a carbohydrate structure influences its ability to maintain microbial diversity in the face of high dilution pressure and 2) whether substrate structuring or obligate microbe-microbe metabolic interactions (e.g. exchange of amino acids or vitamins) exert more influence on maintained diversity. Sorghum arabinoxylan (SAX, complex polysaccharide), inulin (low-complexity oligosaccharide) and their corresponding monosaccharide controls were selected as model carbohydrates. Our results demonstrate that complex carbohydrates stably sustain diverse microbial consortia. Further, very similar final consortia were enriched on SAX from the same individual’s fecal microbiota across a one-month interval, suggesting that polysaccharide structure is more influential than stochastic alterations in microbiome composition in governing the outcomes of sequential batch cultivation experiments. SAX-consuming consortia were anchored by Bacteroides ovatus and retained diverse consortia of >12 OTUs; whereas final inulin-consuming consortia were dominated either by Klebsiella pneumoniae or Bifidobacterium sp. and Escherichia coli. Furthermore, auxotrophic interactions were less influential in structuring microbial consortia consuming SAX than the less-complex inulin. These data suggest that carbohydrate structural complexity affords independent niches that structure fermenting microbial consortia, whereas other metabolic interactions govern the composition of communities fermenting simpler carbohydrates.IMPORTANCEThe mechanisms by which gut microorganisms compete for and cooperate on human-indigestible carbohydrates of varying structural complexity remain unclear. Gaps in this understanding make it challenging to predict the effect of a particular dietary fiber’s structure on the diversity, composition, or function of gut microbiomes, especially with inter-individual variability in diets and microbiomes. Here, we demonstrate that carbohydrate structure governs the diversity of gut microbiota under high dilution pressure, suggesting that such structures may support microbial diversity in vivo. Further, we also demonstrate that carbohydrate polymers are not equivalent in the strength by which they influence community structure and function, and that metabolic interactions among members arising due to auxotrophy exert significant influence on the outcomes of these competitions for simpler polymers. Collectively, these data suggest that large, complex dietary fiber polysaccharides structure the human gut ecosystem in ways that smaller and simpler ones may not.

The role of logistics in managing the system of medical care organization during a pandemic

2021 ◽  
pp. 16-29
Author(s):  
Natalia Guseva
Keyword(s):  
Interpersonal Relations ◽  
Healthcare Management ◽  
Maximum Efficiency ◽  
Extreme Conditions ◽  
Care Organization ◽  
Organization And Management ◽  
Leading Role ◽  
Current Period ◽  
Close Relationship

In the current period of mass coronavirus infection of the population of Russia, hidden defects in the organization and management of healthcare have been exposed. In this regard, the author proposes to take advantage of the unique concept of logistic management, which has the ability to provide any management system with maximum efficiency. Logistics, as a systemically integrated process of material and information flow management, when used correctly, leads to the satisfaction of consumers’ needs with minimal overall costs. This is especially important for the healthcare system operating in the extreme conditions of the COVID19 pandemic. The article focuses on precisely those logistics theses that should be used in healthcare management in a difficult epidemiological situation. The author draws attention to the priority importance of interpersonal relations in medical teams, the leading role of healthcare organizers in solving problems arising in the management of medical organizations in extreme conditions, as well as the close relationship between the concepts of Logistics and «lean medicine»; which, apparently, will serve as the basis for future reforms in the health care sector

scholarly journals Whole Genome Characterization and Genetic Evolution Analysis of a New Ostrich Parvovirus

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 334
Author(s):  
Kunpeng Yuan ◽  
Dongdong Wang ◽  
Qingdong Luan ◽  
Ju Sun ◽  
Qianwen Gao ◽  
...  
Keyword(s):  
Comprehensive Analysis ◽  
Open Reading Frames ◽  
Genome Characterization ◽  
Distance Analysis ◽  
Evolution Analysis ◽  
New Information ◽  
Close Relationship ◽  
Goose Parvovirus ◽  
Genetic Distance Analysis ◽  
Reading Frames

Ostrich diseases characterized by paralysis have been breaking out in broad areas of China since 2015, causing major damage to the ostrich breeding industry in China. This report describes a parvovirus detected in ostriches from four different regions. The entire genomes of four parvovirus strains were sequenced following amplification by PCR, and we conducted comprehensive analysis of the ostrich parvovirus genome. Results showed that the length genomes of the parvovirus contained two open reading frames. Ostrich parvovirus (OsPV) is a branch of goose parvovirus (GPV). Genetic distance analysis revealed a close relationship between the parvovirus and goose parvovirus strains from China, with the closest being the 2016 goose parvovirus RC16 strain from Chongqing. This is the first report of a parvovirus in ostriches. However, whether OsPV is the pathogen of ostrich paralysis remains uncertain. This study contributes new information about the evolution and epidemiology of parvovirus in China, which provides a new way for the study of paralysis in ostriches.

Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 1. Evidence for the Plocamiales ord.nov.

1994 ◽  
Vol 72 (9) ◽  
pp. 1250-1263 ◽  
Author(s):  
G. W. Saunders ◽  
G. T. Kraft
Keyword(s):  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Chain Reaction ◽  
Small Subunit Rrna Gene ◽  
New Information ◽  
The Family ◽  
Close Relationship ◽  
Polymerase Chain

Nucleotide sequences of the nuclear, small-subunit (SSU) ribosomal RNAs, as inferred from polymerase chain reaction (PCR)-amplified products, are presented for Areschougia congesta (Turner) J. Agardh (Solieriaceae), Dasyphloea insignis Montagne (Dumontiaceae), Sarcothalia crassifolia (C. Agardh) Edyvane & Womersley (Gigartinaceae), Nizymenia australis Sonder (Nizymeniaceae), Phacelocarpus peperocarpos (Poiret) Wynne, Ardré & Silva (Phacelocarpaceae), Plocamiocolax pulvinata Setchell, Plocamium angustum (J. Agardh) J.D. Hooker, Plocamium cartilagineum (Linnaeus) Dixon (Plocamiaceae), Rhodymenia linearis J. Agardh (Rhodymeniaceae), and Sphaerococcus coronopifolius Stackhouse (Sphaerococcaceae). Phylogenetic analyses of the SSU sequences between the Plocamiaceae and members of the Sphaerococcaceae, Phacelocarpaceae, and Nizymeniaceae, with which the Plocamiaceae has been associated historically, show SSU differences of between 87 and 105 nucleotides and do not indicate a close relationship. A review of anatomical knowledge of the Plocamiaceae and Pseudoanemoniaceae and new information on vegetative and tetrasporangial development in Plocamium and Plocamiocolax are presented to buttress a case for the Plocamiales ord.nov. Representatives of the Nizymeniaceae and Phacelocarpaceae differ from one another by only nine nucleotides, suggesting that these two taxa are very closely related and perhaps not distinct at the family rank. Key words: Gigartinales, PCR, phylogeny, Plocamiales ord.nov., Pseudoanemoniaceae, Rhodophyta, small-subunit rRNA, systematics.

scholarly journals ФОРМА ЯК ЗАСІБ СТВОРЕННЯ ХУДОЖНЬОГО ОБРАЗУ В АРТ-ДИЗАЙНІ

2019 ◽  
pp. 40-47
Author(s):  
Н. Л. Базелюк
Keyword(s):  
Design Theory ◽  
Theory And Practice ◽  
Practical Significance ◽  
Art Object ◽  
Art Form ◽  
Close Relationship ◽  
Functional Features ◽  
Characteristic Features ◽  
Object Form ◽  
Artistic Image

Purpose. Disclosure of decorative and functional features of forms of art object and analysis of their creative and aesthetic content. Methodology. In the course of the research, the purpose of field surveys and generally scientific methods of analysis, synthesis and analogies were used. Results. As a result of a theoretical study, the primary stages of art-form object formation are determined; its characteristic features and peculiarities of ideological and ideological ideas and creative language are revealed. The close relationship between form and content, which forms an imaginative, symbolic, spiritual and aesthetic art object with the emotional power of influence and impression, is considered. Scientific novelty. The article analyses and explores the peculiarities of creating an art object form to convey an artistic image and an author's idea. The features and stages of forming an art object form as a complex, multifaceted phenomenon are presented, which allows becoming more aware of its functional, material and artistic features. Practical significance. Research findings can be used as promising areas for research in design theory and practice. The main defining features and features of building an art object can be applied directly in the creative work of designers.

A new troodontid (Dinosauria, Theropoda) braincase from the Dinosaur Park Formation (Campanian) of Alberta

1993 ◽  
Vol 30 (10) ◽  
pp. 2231-2247 ◽  
Author(s):  
Philip J. Currie ◽  
Xi-Jin Zhao
Keyword(s):  
Inner Ear ◽  
Computerized Tomography ◽  
Middle Ear ◽  
Lateral Surface ◽  
Ct Scans ◽  
Internal Anatomy ◽  
New Information ◽  
Close Relationship ◽  
Dinosaur Park Formation ◽  
The Brain

A new, well-preserved specimen of Troodon formosus is the first to reveal the internal anatomy of the lower part of the braincase. In addition to providing new information on the brain of this highly encephalized dinosaur, the uncrushed bones clear up anatomical details left obscure by earlier studies. Computerized tomography (CT) scans reveal the nature of the inner ear and the course of the pneumatic ducts diverging from the middle ear. Evidence is presented to show that four of the five periotic pneumatic systems found in bird skulls are present in Troodon. The anterior tympanic recess is the most elaborate system, and diverticula from each side extend anteriorly, dorsally and, posteriorly from the middle ear. The posterior tympanic recess is located within the paroccipital process and the basioccipital, but the pneumatopore posterolateral to the stapedial recess is secondarily closed. The dorsal periotic sinus is represented by a smooth-surfaced concavity on the lateral surface of the prootic. The position of a pneumatic recess in this region is demonstrated by the presence of a pneumatopore in the quadrate. Diverticula from the anterior and posterior tympanic recesses are connected within braincase bones, and a possible pneumatopore in the prootic may connect these to the dorsal tympanic recess. The pneumatic condition of the troodontid articular is unknown. Contralateral connections of the sinus systems have been used to argue for a close relationship between birds and crocodiles, but their presence in this specimen suggests that they appeared more than once in archosaurs or that they are plesiomorphic for crocodiles, dinosaurs, and birds. Cranial pneumaticity cannot be used by itself to resolve the interrelationships of crocodiles, theropods, and birds, but other characters suggest derivation of birds from theropods.

scholarly journals EXPLICIT AND IMPLICIT MEANS OF IMPLEMENTING THE VOLUNTATIVE FUNCTION OF A LANGUAGE IN GERMAN AND OSSETIAN LINGUOCULTURES: TO THE PROBLEM OF THE NATIONAL PICTURE OF THE WORLD

2021 ◽  
pp. 99-106
Author(s):  
Д.М. ДРЕЕВА ◽  
Д.В. ТОЛПАРОВА
Keyword(s):  
Dominant Form ◽  
Language And Culture ◽  
The World ◽  
Close Relationship ◽  
Poetic Text ◽  
Functional Features ◽  
Characteristic Features ◽  
Poetic Texts ◽  
Relationship Of ◽  
Empirical Material

Настоящее исследование посвящено выявлению структурно-семантических и функциональных особенностей средств выражения категории побуждения на примере анализа употребления эксплицитных и имплицитных форм реализации волюнтативной функции языка в поэтических произведениях австрийской поэтессы Ингеборг Бахман и осетинского поэта Нафи Джусойты. Способы реализации категории побуждения в поэтическом тексте рассматриваются сквозь призму немецкоязычной и осетинской лингвокультур. Выбор лингвокультурологического подхода к изучению прямых и косвенных форм вербализации волюнтативной функции в стихотворных текстах объясняется тесной взаимосвязью языка и культуры, носителем которых является поэт. Картина мира, включающая в себя определенные представления об окружающей действительности, обусловливает характерные особенности идиостиля автора, детерминируя выбор тех или иных художественных средств. Анализ средств выражения волеизъявления позволил сделать вывод о высокой плотности употребления форм повелительного наклонения в поэтических произведениях И. Бахман и Н. Джусойты. В частности, отмечается более широкое использование грамматически маркированной формы – императива – в текстах обоих авторов. По результатам проведенного исследования императив может быть квалифицирован как доминантная (эталонная) форма выражения категории побуждения как в немецком, так и в осетинском языках. Дальнейшее изучение стихотворных произведений указанных авторов выявило преобладание прямых средств репрезентации волюнтативной семантики в текстах Бахман и индиректных форм – в стихотворных произведениях Джусойты. Данное наблюдение позволяет в рамках нашего исследования сделать предварительный вывод о различиях в немецкоязычной и осетинской лингвокультурах, который, безусловно, нуждается в подтверждении на основе анализа более обширного эмпирического материала. Следовательно, на основании проведенного исследования можно заключить, что для наиболее полного и корректного изучения национальной картины мира необходим анализ идиостиля поэта на разных языковых уровнях – прагматики, семантики и грамматики. The present study is devoted to the identification of structural, semantic and functional features of the expression’s means of the motivation’s category by the example of the analysis of the using explicit and implicit forms of the implementation of the voluntative function of a language in the poetic works of the Austrian poetess Ingeborg Bachman and the Ossetian poet Nafi Dzhusoity. The ways of implementing the category of motivation in a poetic text are considered through the prism of German- and Ossetian-speaking linguocultures. The choice of a linguoculturological approach to the study of direct and indirect forms of verbalization of the voluntative function in poetic texts is explained by the close relationship of language and culture. The picture of the world, which includes certain ideas about the surrounding reality, determines the characteristic features of the author's idiostyle, determining the choice of certain artistic means. The analysis of the means of will’s expression allowed us to conclude about the high density of the use of imperative forms in the poetic works of I. Bakhman and N. Dzhusoity. In particular, there is a wider use of the grammatically marked form – imperative – in the texts of both authors. According to the results of the study, the imperative can be qualified as the dominant form of expression of the motivation’s category in both German and Ossetian languages. Further study of the poetic works of these authors revealed the predominance of direct means of representation of voluntary semantics in the texts of I. Bakhman and indirect forms – in the poetic works of N. Dzhusoity. This observation allows us to make a preliminary conclusion about the differences in the German- and Ossetian-speaking linguocultures, which, of course, needs confirmation based on the analysis of more extensive empirical material. Therefore, basing on the conducted research, we can conclude that for the most complete and correct study of the national picture of the world, an analysis of the poet's idiostyle at different language levels – pragmatics, semantics and grammar – is necessary.

scholarly journals Sound Velocity Measurement of Shock-Compressed Quartz at Extreme Conditions

Minerals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1334
Author(s):  
Liang Sun ◽  
Huan Zhang ◽  
Zanyang Guan ◽  
Weiming Yang ◽  
Youjun Zhang ◽  
...  
Keyword(s):  
Sound Velocity ◽  
Melting Process ◽  
Extreme Conditions ◽  
Planetary Interiors ◽  
New Information ◽  
Laser Facility ◽  
Magnesium Silicates ◽  
Magma Oceans ◽  
Physical And Chemical ◽  
Key Parameter

The physical properties of basic minerals such as magnesium silicates, oxides, and silica at extreme conditions, up to 1000 s of GPa, are crucial to understand the behaviors of magma oceans and melting in Super-Earths discovered to data. Their sound velocity at the conditions relevant to the Super-Earth’s mantle is a key parameter for melting process in determining the physical and chemical evolution of planetary interiors. In this article, we used laser indirectly driven shock compression for quartz to document the sound velocity of quartz at pressures of 270 GPa to 870 GPa during lateral unloadings in a high-power laser facility in China. These measurements demonstrate and improve the technique proposed by Li et al. [PRL 120, 215703 (2018)] to determine the sound velocity. The results compare favorably to the SESAME EoS table and previous data. The Grüneisen parameter at extreme conditions was also calculated from sound velocity data. The data presented in our experiment also provide new information on sound velocity to support the dissociation and metallization for liquid quartz at extreme conditions.

Hind limb osteology of Gracilisuchus stipanicicorum (Archosauria: Pseudosuchia)

2012 ◽  
Vol 102 (2) ◽  
pp. 105-128 ◽  
Author(s):  
A. Lecuona ◽  
J. B. Desojo
Keyword(s):  
Femoral Head ◽  
Hind Limb ◽  
Detailed Examination ◽  
Pelvic Girdle ◽  
The Other ◽  
Late Triassic ◽  
New Information ◽  
Close Relationship ◽  
Phylogenetic Affinities ◽  
Relationship Of

ABSTRACTGracilisuchus stipanicicorum Romer, 1972, from the Middle-Late Triassic of the Ischigualasto–Villa Unión Basin of Argentina, is an extinct pseudosuchian archosaur on the stem to Crocodylomorpha. The pelvic girdle and hind limb anatomy of a referred specimen of Gracilisuchus stipanicicorum is described and compared with that from a broad range of archosauriform taxa, including basal members such as crurotarsans and basal ornithodirans. The description of this specimen reveals new information on the anatomy of the pelvic girdle and hind limb of Gracilisuchus, through a detailed examination of some anatomical regions barely or not previously described, as well as reinterpretations of previous features. The phylogenetic affinities of Gracilisuchus within the Archosauria remain to be tested, but Gracilisuchus shares two putative synapomorphies with some non-crocodyliform crocodylomorphs, providing tentative support for the monophyly of Sphenosuchia (e.g., Sereno & Wild 1992; Wu & Chatterjee 1993) and the close relationship of Gracilisuchus to that clade. These characteristics are: (i) the morphology and poor development of the femoral fourth trochanter, closely resembling the condition of Pseudhesperosuchus and Trialestes; and (ii) a poor anterior development of the femoral head, shared with Pseudhesperosuchus. On the other hand there are characters that reject the inclusion of Gracilisuchus within Crocodylomorpha (Nesbitt 2011), such as the absence of an imperforated acetabulum, and that rather suggests a sister-taxon position to Crocodylomorpha.

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