scholarly journals Effect of coagulase-negative staphylococci on colostrum composition, properties and fatty acid profile

Author(s):  
Alina Pikhtirova ◽  
Ewa Pecka-Kiełb ◽  
Andrzej Zachwieja ◽  
Jolanta Bujok ◽  
František Zigo

Abstract Background: Colostrum is the first food for mammals that should give a head start to the newborn organism. By providing the body with essential nutrients, colostrum plays an immune and immunostimulating function. Colostrum quality depends on multiple factors, including microbial effect. Widespread coagulase-negative staphylococci have a great impact on colostrum qualitative indicators. The aim of this study was to explore the effect of staphylococci on colostrum quality. Results: Physical, chemical properties and fatty acid profile of cow’s colostrum were determined. In our study we identified three pathogenic coagulase-negative Staphylococcus species in the cows’ colostrum: S. sciuri (64.29 %), S. xylosus (28.57 %) and S. warneri (7.14 %). We noted that the somatic cell count (SCC) in the affected colostrum samples wasn’t noticeably higher than that in the uninfected colostrum samples. Percentage of dry matter in infected and uninfected colostrum samples was nearly the same. Contents of fat, protein, and lactose in the studied colostrum were not significantly different. The content of butyric (С4:0) and capric (С10:0) acids was significantly higher (P<0.05) in the colostrum fat of cows infected with CNS compared to that of the uninfected animals. Conclusions: According to our research infection with coagulase-negative staphylococci negatively affects colostrum patameters primarily due to an increase in SCC, acidity and decrease in β-casein level. Moreover, significant decrease in total bacterial count in infected by CNS samples may be caused by a decrease in the level of beneficial microflora. Minor changes in fatty acid profile of fat in colostrum with CNS do not have a significant negative effect on colostrum as a whole. But in view of the fact that colostrum is intended for feeding a newborn with a "sterile" gastrointestinal tract, the presence of CNS will undoubtedly leads to a great threat to an immature organism.

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Maricel Andrea Gallardo ◽  
Héctor José Milisich ◽  
Silvina Rosa Drago ◽  
Rolando José González

In order to determine the effect of cultivars and planting date on flax fatty acid profile, seed yield, and oil content, an assay with seven cultivars (Baikal, Prointa Lucero, Prointa Ceibal, Panambí INTA, Curundú INTA, Carapé INTA, and Tape INTA) was carried out at Parana Agricultural Experimental Station, Argentina. Significant differences among cultivars were found for content of palmitic (5–7 g/100 g), stearic (5–8 g/100 g), linoleic (13–19 g/100 g), saturated (11–15 g/100 g), and unsaturated acids (92–96 g/100 g) within the seven cultivars. The best seed yields were observed in Prointa Lucero and Carapé INTA varieties (2091.50 kg·ha−1and 2183.34 kg·ha−1, respectively) in the first planting date and in Carapé INTA and Prointa Lucero (1667 kg·ha−1and 1886 kg·ha−1, respectively) in the second planting date. A delayed planting date had a negative effect on seed yield (1950 kg·ha−1and 1516 kg·ha−1) and oil content (845 kg·ha−1and 644 kg·ha−1) but did not affect oil composition.


2012 ◽  
Vol 36 (0A) ◽  
pp. 151-156
Author(s):  
Dahfier Abid Ali Al-Obaidi

This study was carried out at the College of VeterinaryMedicine, University of Baghdad,for two months, to study the beef luncheon meat in Baghdad city by evalutuation somePhysicochemical and bacteriological features the obtained data revealed the following results:Physicochemical indicated that myoglobin concentration, was low for all sample of meat withno significant differences in marks which were 163.3 – 178.7 mg/100g meat. Total volatilenitrogen (TVN) values were more the allowance limits especially in food and mark whichwere 20.4mgN/100g meat with no significant differences in marks. Thiobarbituric acid (TBA)values and Free Fatty acid (FFA) percentages were withen the allowance limits in all marks.Bacteriological results indicated that total Bacterial count (TPC) were low of meat samplewhich were140 - 220 cfu /g, with significant differences (P<0.05) in Marks. The total coliformcount were less than allowance limits, were the same with Anaerobic total Bacterial count(A.T.P.C.) which Were 3 - 41cfu/g and30 – 260 cfu/g with significant differences (P<0.05) inmarks. The results indicated that beef luncheon was low in some Physicochemicalespecially in food land mark. Therefore it is necessary to make Evaluation cautiously of theimported meats and meat processing to safe public health.


2016 ◽  
Vol 83 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Dea Anton ◽  
Piret Raudsepp ◽  
Mati Roasto ◽  
Kadrin Meremäe ◽  
Sirje Kuusik ◽  
...  

In the current study the microbiological, sensory and chemical properties of 24 kefirs (12 producers) from Estonian, Latvian and Lithuanian retail market were determined using gas chromatography (GC), high performance liquid chromatography (HPLC-MS/MS-Q-TOF and LC-ion trap MS/MS), spectrophotometry and other methods. Antihypertensive, angiotensin-converting enzyme (ACE) inhibiting, antioxidant and antibacterial peptides were found in the kefir samples. According to the results of principal component analysis of 200 most abundant compounds obtained with HPLC-MS/MS-Q-TOF analysis, Estonian kefirs differed from the rest. Kefirs of Latvian and Lithuanian origin showed similarities in several characteristics, probably related to the starter cultures and technological processes. The fatty acids composition of all Baltic kefirs was uniform. The antioxidant capacity of the kefirs varied slightly, whereas intermediate positive correlation (r= 0·32,P< 0·05) was found between antioxidativity and total bacterial count. The lipid oxidation level, estimated as the content of linoleic and oleic acid primary oxidation products, oxylipins, was very low in all studied kefirs. Only one third of analysed kefirs met the requirements of the minimum sum of viable microorganisms, indicated in the Codex Standard for Fermented Milks.


2016 ◽  
Vol 4 (1) ◽  
pp. 4
Author(s):  
Hayat Hassan ◽  
Hatil EL-Kamali

<p>Total viable counts of bacteria and bacterial diversity of the different soil samples from three different localities in Sharq EL-Neel region: Soba, AL-Aelafoon and Um Dawan Ban sub-regions were carried out. Soil physical and chemical characteristics (pH, EC,SP,solublecations: Na, K, Ca, Mg and anion P, organic carbon, total nitrogen and soil texture)in each studied sub-regions were measured. Qualitative analysis of microorganisms isolated from the studied soil samples reveal a total of thirteendifferent species of bacteria, of which two are unidentified. The ten species are classified under Bacillus genus. In Sharq EL-Neel regionsoil samples, total bacterial counts ranged from 9.5 × 10<sup>4 </sup>cfu g<sup>-1</sup> to 1×10<sup>3</sup> with a mean of 4×10<sup>3 </sup>cfu g<sup>-1</sup>. The quantitative data on microbial population recorded in the present study was analysed using two diversity indices. High Shannon-Weiner diversity Index value for bacteria was obtained in AL-Aelfoon sub-region (1.79361), whereas high Simpson's index value was obtained in Um Dawan Ban sub-region (2.80).ActinomycesActinomyces spp. and Streptomyces spp. Where the most abundant microorganisms identified in the three sub-regions. Total bacterial count in Soba soil was positively correlated with pH (r= 0.0194) and sand (r= 0.3205); the total bacterial count in AL-Aelafoon soilwas positively correlated with EC (r= 0.1062), clay (r= 0.3816), silt (r= 0.1936), SP (r= 0.9302), K (r= 0.6252), Ca (r= 0.0015) and Mg (r= 0.1556), whereas the total bacterial count in Um Dawan Ban soil was positively correlated with clay (r= 0.2614), silt (r= 0.0216), SP (r= 0.565), K (r= 0.9645), P (r= 0.0197), Ca (r= 0.7377), Mg (r= 0.0267), N (r= 0.5215) and O.C (r= 0.3214). There were obvious differences in correlation coefficients among the selected criteria (46 % from the total number of correlation coefficients were positively correlated between bacterial counts and soil physico-chemical properties whereas 54% from the total number were positively correlated between plant type and bacterial counts).</p>


2011 ◽  
Vol 8 (1) ◽  
pp. 35-38
Author(s):  
Baghdad Science Journal

Three stations were chosen on the water treatment plan of al- madaan .The Samples collected from the (Raw water) and the Sedimentation, filtration and storage water and the drinking water of outlet. Coliform densities T.S and F.C and TS and F.S and total bacterial count as bacteriological pollution indicators, as moste probable number (MPN) method was studied in test. Also some of the chemical characteristics of the water like pH , total suspended solid T.S.S, T.D.D.and S04 , T.Hardness , Ca++ , Mg++ . From the results it were indicated . The study showed the drinking water of outlet (distriputed in system) was agree with WHO criteria and Iraqi limits standards .


Author(s):  
Meda Mruthyumjaya Rao ◽  
P. Hemant Kumar ◽  
Purnendu Panda ◽  
Sangeeta Mukhi ◽  
Anindya Bose

Madhuyashti Ghrita, is an Ayurvedic medicated ghee preparation containing Yashtimadhu. It is used for treating external ulcers and wounds in vital points of the body. However, there is no quality monograph available for Madhuyashti Ghrita. Hence, the present work was carried out to characterize this Ghrita to confirm its identity, quality and purity. This work reported various pharmacognostic and physicochemical parameters of Madhuyashti Ghrita along with its TLC based rapid fingerprinting as per the present standards of Ayurvedic Pharmacopoeia of India. Moreover, the presence of toxic contaminants like heavy metals, and microbial load were also evaluated. In organoleptic evaluation, the Ghrita was found to be semi–solid and dark yellow in colour, sweet and bitter in taste with a characteristic and pleasant odour. The values of the physicochemical parameters such as acid value, saponification value, peroxide value, refractive index, rancidity and pH value of the Ghrita were also assed. In the microscopical analysis of the rhizomes of Yashtimadhu, the presence of pitted vessels, fibres, prismatic crystals and starch grains were noted as a confirmation characteristic for the genuinely of this raw material in Madhuyashti Ghrita. The amount of heavy metals such arsenic, lead, mercury, cadmium, nickel, zinc, copper and chromium as well as total bacterial count and total fungal count were found to be much below their API limits. The TLC showed different characteristic spots indicating the presence of Yashtimadhu in this product. The parameters of Madhuyashti Ghrita presented in this paper may be utilized for preparing a quality monograph for this product.


2010 ◽  
Vol 4 (1) ◽  
pp. 65-71
Author(s):  
Saddia M. AL-Jumayli ◽  
Salim S. AL-Timimi

Many kind of market – available frozen meats were selected. These involves Iraqi meat, Emirate meat as well as four types of Indian meats ( Amarona, Alana, Al – Mubark and Al – Halal ) The chemical, Physical and Microbial characteristics of these types were investigated. All studies types were identical with [1] in protein percentage. Excluding data of fat percentage of Emirale meat, all types were identical with [1] in fat percentage. Significant differences in free fatty acid percentage were observed in studied types, however it still in accordance with [1]. Highly significant (P>0.05) differences were noticed among meat types in total volatile nitrogen values. On the other hand, all meat types were not identical with Iraqi standards in thaw loss percentage; all sensory characters were decline in all frozen meat studied. Greater total aerobic and total bacterial count were noticed in all meat types. These exceeded that of [1]. On the same manar, Higher count of Psychotropic bacteria (5.12 x 104 ) were observed in Indian meat (Halal).


2012 ◽  
Vol 42 (4) ◽  
pp. 691-696 ◽  
Author(s):  
João Emídio Ferreira Lopes Júnior ◽  
Carla Cristine Lange ◽  
Maria Aparecida Vasconcelos Paiva Brito ◽  
Fabiana Ribeiro Santos ◽  
Marco Aurélio Souto Silva ◽  
...  

This study was conducted to establish the relationship between somatic cell count (SCC) and bacterial shedding from mammary quarters according to mastitis pathogens. Milk samples from 638 mammary quarters were examined for mastitis pathogens, SCC and total bacterial count (TBC). The raw data of SCC and TBC were used to perform descriptive statistics. The significance of the arithmetic mean differences between SCC and TBC according to bacteriological examination results was determined by a two-tailed unpaired t-test. Pearson and Spearman´s correlations were done with logarithmic data and linear regression analyses. The geometric means of the bacteriological examination results were (cells mL-1; CFU mL-1): no growth (52,000; 12,000), coagulase-negative staphylococci (85,000; 17,000), Staphylococcus aureus (587,000; 77,000); other streptococci (432,000; 108,000) and Streptococcus agalactiae (1,572,000; 333,000). The Pearson and Spearman's correlations between SCC and TBC were higher than 0.60 for all mastitis pathogens. The regression analyses slopes showed different increase in TBC with the same increase in SCC according to mastitis pathogens. The slope for S. agalactiae (0.542) was higher than that for other mastitis pathogens. The results suggest that the intensity of inflammatory process was associated with number of mastitis pathogens shedding from the mammary gland.


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