Model study on chicken embryos to investigate potential teratogenic risk of wild birds due to pesticide and environmental contaminant

2021 ◽  
Author(s):  
József Lehel ◽  
Géza Szemerédy ◽  
Rita Szabó ◽  
László Major ◽  
Adrienn Grúz ◽  
...  
Keyword(s):  
Body Weight ◽  
Copper Sulphate ◽  
The Body ◽  
Immersion Method ◽  
Developmental Abnormalities ◽  
Chicken Embryos ◽  
Additive Type ◽  
Model Study ◽  
Air Chamber ◽  
Wild Life

Abstract Single and simultaneous toxic effects of glyphosate (Amega Up, 360 g/L, 4%) and copper sulphate (0.01%) were studied on avian embryos treated with injection directly into the air chamber or by immersion application for 30 minutes on day 0 of incubation. Alterations of the chicken embryos were evaluated during the necropsy performed on day 19 of incubation including the mortality, the body weight, and the type of developmental abnormalities. Based on the results, the injection application was to be more toxic than the immersion method, induced increasing of mortality and decreasing of body weight, and the incidence of the congenital anomalies was more frequent. Supposedly, an additive-type toxicodynamic interaction was occurred between the copper sulphate and glyphosate that may result in reduced vitality of the embryos and thus the number of offspring of wild-life birds.

scholarly journals Reproductive toxicological changes in avian embryos due to a pesticide and an environmental contaminant

2021 ◽  
Vol 69 (4) ◽  
pp. 363-371
Keyword(s):  
Body Weight ◽  
Congenital Anomalies ◽  
Copper Sulphate ◽  
Wild Birds ◽  
Immersion Method ◽  
Developmental Abnormalities ◽  
Avian Embryos ◽  
Air Chamber ◽  
Average Body ◽  
Average Body Weight

Abstract Single and simultaneous toxic effects of glyphosate (Amega Up, 360 g L−1, 4%) and copper sulphate (0.01%) were studied in avian embryos treated either with injection directly into the air chamber or by immersion application for 30 min on day 0 of incubation. Alterations of the chicken embryos were evaluated during necropsy performed on day 19 of incubation, together with mortality, body weight and the type of developmental abnormalities. Based on the results, the injection application appeared to be more toxic than the immersion method, as it induced increased mortality and reduced the average body weight, and resulted in a higher incidence of congenital anomalies. Supposedly, a toxicodynamic interaction occurs between copper sulphate and glyphosate, which may reduce the vitality of embryos and thus decrease the number of offspring in wild birds.

scholarly journals MODEL STUDY TO INVESTIGATE THE TOXIC INTERACTION BETWEEN GLYPHOSATE AND COPPER SULPHATE ON CHICKEN EMBRYOS

AGROFOR ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Rita SZABÓ ◽  
Péter BUDAI ◽  
Éva KORMOS ◽  
István BUDA ◽  
Adrienn GRÚZ ◽  
...  
Keyword(s):  
Copper Sulphate ◽  
Osmium Tetroxide ◽  
Embryonic Mortality ◽  
Early Developmental Stage ◽  
Developmental Abnormalities ◽  
Chicken Embryos ◽  
Model Study ◽  
Air Chamber ◽  
Early Embryonic Mortality ◽  
Early Developmental

The toxic effects of the Taifun Forte herbicide (360 g/l glyphosate isopropylamine salt) applied alone or in combination with copper sulphate were studied on chicken embryos in the early phase of embryonic development. The test materials were injected in 0.1 ml volume into the air chamber of eggs on the first day of incubation. Subsequently, on the third day of incubation permanent preparations were made from the embryo in order to study the early developmental stage. Embryos fixed on slides and stained with osmium tetroxide solution were studied under light microscope. The embryonic mortality and the developmental anomalies was analysed statistically by Fisher test. According to the result of the statistical evaluation, the embryonic mortality was not influenced by the single treatment of copper sulphate. However, Taifun Forte and its combination with heavy metal significantly increased the early embryonic mortality. Developmental abnormalities were sporadically observed due to the single administration of copper sulphate. The incidence of it was increased due to the treatment with herbicide alone and in combination with copper sulphate. Based on the results, additive toxic interaction may occur between the copper sulphate and glyphosate that can highly reduce the viability of the embryos or can lead to extinction of wild birds in serious cases.

scholarly journals TOXICITY TEST OF INDIVIDUAL AND COMBINED TOXIC EFFECTS OF GLYPHOSATE HERBICIDE AND HEAVY METALS ON CHICKEN EMBRYOS

AGROFOR ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Rita SZABÓ ◽  
Dalma CSONKA ◽  
László MAJOR ◽  
József LEHEL ◽  
Péter BUDAI
Keyword(s):  
Heavy Metals ◽  
Body Weight ◽  
The Body ◽  
Embryo Mortality ◽  
Developmental Anomalies ◽  
Chicken Embryos ◽  
Post Test ◽  
Test Materials ◽  
The Individual ◽  
Chicken Eggs

The aim of this study was to determine the individual and combined toxicity of Glialka Star herbicide (glyphosate 360 g/l) and heavy metals (copper and cadmium) on the development of chicken embryos. On the first day of incubation, chicken eggs were injected by 0.1 ml of the test materials. The applied concentration of copper and cadmium sulphate was 0.01% and that of herbicide Glialka Star was 2%. The chicken embryos were examined on day 19 by the followings: rate of embryo mortality, body mass, type of developmental anomalies by macroscopic examination. The body weight was evaluated statistically by oneway ANOVA combined with Dunnett post-test, the embryo mortality and the developmental anomalies were analysed by Fisher test. Our teratogenicity study revealed that the combined administration of heavy metals (copper, cadmium) and glyphosate (K-salt) containing herbicide formulation caused significant reduction in the body weight of embryos and a significant increase in the rate of embryonic mortality and the incidence of developmental anomalies. The joint toxic effect of heavy metals and Glialka Star is an additive effect compared to the individual toxicity of the test materials.

scholarly journals STUDIES ON JOINT TOXIC EFFECTS OF A GLYPHOSATE HERBICIDE (FOZÁT 480) AND A HEAVY METAL (CADMIUM) ON CHICKEN EMBRYOS

AGROFOR ◽  
2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Rita SZABÓ ◽  
Géza SZEMERÉDY ◽  
Éva KORMOS ◽  
József LEHEL ◽  
Péter BUDAI
Keyword(s):  
Body Weight ◽  
The Body ◽  
Toxic Effects ◽  
Chicken Embryos ◽  
Body Weight Data ◽  
Different Types ◽  
Glyphosate Herbicide ◽  
The Individual ◽  
Chicken Eggs ◽  
Weight Data

The aim of the study was to determine the individual and combined toxic effects ofthe herbicide Fozát 480 (glyphosate [isopropylamine salt] 480g/l) and cadmiumsulphate (CdSO4) on the development of chicken embryos. On the first day ofincubation, chicken eggs were injected with 0.1 ml of cadmium sulphate solution(0.1%) and/or with 0.1 ml of Fozát 480 (2.0%). The chicken embryos wereexamined on the 19th day by measuring the rate of embryo mortality and bodyweight and by identification of different types of developmental anomalies andmacroscopic malformations. The body weight data were statistically evaluated byone-way ANOVA and Dunnett tests, while the embryonic mortality and thedevelopmental anomalies were analysed by Fisher test. Our teratogenicity studyrevealed, that the combined administration of cadmium sulphate and glyphosate(isopropylamine salt) containing herbicide formulation caused a significantreduction in the body weight of embryos and increased the rate of embryonicmortality. The joint toxic effect of cadmium sulphate and Fozát 480 is an additiveeffect compared to the individual toxicity of the test materials.

scholarly journals Comparative characteristics of morphometric indicators of growth and development of egg-type chicken embryos at different stages of embryogenesis

2021 ◽  
Author(s):  
M.I. Chelnokova ◽  
A.A. Chelnokov ◽  
F.I. Suleymanov
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Body Mass ◽  
Body Length ◽  
Growth And Development ◽  
The Body ◽  
Specific Rate ◽  
Chicken Embryos ◽  
Stage Of Development ◽  
Fetal Stage

The article presents a comparative analysis of morphometric parameters of growth and development of chicken embryos from hybrid poultry egg productivity at different stages of embryogenesis. In the embryos of Loman Brown, compared with Haysex Brown, there was a statistically significant increase in absolute values of length and body weight at all stages of embryonic development. In late-fetal stage length and weight body of embryos, Lohmann Brown was more on 0,339 cm (P<0,001) and 0,167 g (P<0,01), in early-fetal stage - on 0,713 cm (P<0,01) and 1,282 g (P<0,01), in mid-fetal stage - on 0,673 cm (P<0,001) and 4,226 g (P<0,001), in late-fetal stage - on 0,245 cm (P<0,001) and 6,437 g (P<0,05), respectively. By the late-fetal stage of development, the specific growth rate of length and mass in chicken embryos of both crosses decreased. The specific rate of body length is statistically significantly higher by 0.099% (P<0,05) in the mid-fetal stage and by 0.171% (P<0,001) in the late-fetal stage of Haysex Brown embryos. Specific body mass sizes in the late-fetal stage of cross Haysex Brown embryos are 3.356% larger (P<0,01) than in Loman Brown embryos. At all stages of embryogenesis, the growth rate of the body length of the Haysex Brown and Loman Brown embryos is characterized by negative allometry. Slower growth in length relative to body weight in the late embryonic stage is observed in the embryos of Haysex Brown (b=0,500). In subsequent stages of development, chicken embryos of two different crosses show the same negative allometric growth in body mass length relative to their weight.

L-Ascorbic Acid Addition to Chitosan Reduces Body Weight in Overweight Women

2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Fat Body ◽  
Body Weight Gain ◽  
Skinfold Thickness ◽  
The Body ◽  
Control Group ◽  
Percentage Body Fat ◽  
Overweight Women ◽  
Body Weights

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.

Hand-assisted laparoscopic nephrectomy in morbidly obese patients

2020 ◽  
Vol 99 (6) ◽  
pp. 271-276
Keyword(s):  
Body Weight ◽  
Surgical Technique ◽  
Blood Loss ◽  
Hospital Stay ◽  
Kidney Cancer ◽  
The Body ◽  
Laparoscopic Nephrectomy ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Morbid Obese

Introduction: Prevalence of obesity is 30 % in the Czech Republic and is expected to increase further in the future. This disease complicates surgical procedures but also the postoperative period. The aim of our paper is to present the surgical technique called hand-assisted laparoscopic nephrectomy (HALS), used in surgical management of kidney cancer in morbid obese patients with BMI >40 kg/m2. Methods: The basic cohort of seven patients with BMI >40 undergoing HALS nephrectomy was retrospectively evaluated. Demographic data were analyzed (age, gender, body weight, height, BMI and comorbidities). The perioperative course (surgery time, blood loss, ICU time, hospital stay and early complications), tumor characteristics (histology, TNM classification, tumor size, removed kidney size) and postoperative follow-up were evaluated. Results: The patient age was 38−67 years; the cohort included 2 females and 5 males, the body weight was 117−155 kg and the BMI was 40.3−501 kg/m2. Surgery time was 73−98 minutes, blood loss was 20−450 ml, and hospital stay was 5−7 days; incisional hernia occurred in one patient. Kidney cancer was confirmed in all cases, 48–110 mm in diameter, and the largest removed specimen size was 210×140×130 mm. One patient died just 9 months after the surgery because of metastatic disease; the tumor-free period in the other patients currently varies between 1 and 5 years. Conclusion: HALS nephrectomy seems to be a suitable and safe surgical technique in complicated patients like these morbid obese patients. HALS nephrectomy provides acceptable surgical and oncological results.

HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

2020 ◽  
Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  
Keyword(s):  
Body Weight ◽  
Histological Examination ◽  
Aflatoxin B1 ◽  
Structural Changes ◽  
Clinical Manifestations ◽  
Feed Additives ◽  
The Body ◽  
Histological Changes ◽  
Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

Defensive activity of Hesperidin against Ciprofloxacin induced hepatic injury in rabbits.

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Hawraa M. Murad ◽  
Tamadhur Hani Hussein ◽  
Audai Sulaiman Khudhair ◽  
Manal Muhi Murad ◽  
Jawad Kadhim Faris
Keyword(s):  
Body Weight ◽  
Bacterial Infections ◽  
Hepatoprotective Activity ◽  
The Body ◽  
Local Breed ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Defensive Activity ◽  
Antioxidant Effects

This study was conducted to find out hepatoprotective activity of hesperidin (HES) 100mg/kg body weight (b.w.) against ciprofloxacin (CPX) 100 mg/kg induced hepatotoxicity in local breed rabbits .CPX is a broad spectrum antibiotic used for treatment of many bacterial infections. Twenty four male rabbits were divided into four groups ,group1: control, (1 ml/kg Saline orally) group 2: CPX (100 mg/kg orally) for (14) consecutive days , group 3: HES (100 mg//kg) orally for (14) consecutive days group 4: CPX (100 mg/kg orally) plus HES (100 mg//kg orally ) for (14) consecutive days. All the rabbits were killed on the (15) day of the experiment, and then the blood, and livers samples were taken. CPX induced hepatotoxicity was proved by a significant (p less than 0.01) reduction in the body weight ,and a significant (p less than 0.01) increased serum aspartate transaminase (AST), alanine transaminase (ALT) , Malonaldehyde enzyme (MAD) and histopathological changes. Protective hepatic toxicity effect and oxidative damage caused by CPX significantly (p less than 0.01) increasing in body weight and significantly (p less than 0.01) decreasing AST , ALT, MAD and improving tissue morphology in HES (100 mg//kg) . These results assure that HES (100 mg//kg) antioxidant effects can protect CPX-induced hepatotoxicity in rabbits.

Efficacy and safety of drospirenone-containing microdosage combined oral contraceptive use with starting contraception

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 9-13
Author(s):  
A R Khachaturian ◽  
E V Misharina ◽  
M I Yarmolinskaya
Keyword(s):  
Blood Pressure ◽  
Body Weight ◽  
Oral Contraceptive ◽  
Waist Circumference ◽  
Young Women ◽  
Iron Metabolism ◽  
Serum Iron ◽  
The Body ◽  
Menstrual Bleeding ◽  
Combined Oral Contraceptive

Androgen-dependent dermopathy, as well as premenstrual syndrome of varying severity in young women, can cause emotional depression, difficulties in social adaptation and even depressive disorders. The aim of the study was to study the safety and efficacy of using a combined oral contraceptive (COC) Dimia® containing 20 μg ethinyl estradiol and 3 mg drospirenone in young women, as well as its therapeutic effects in androgen-dependent dermopathy. Materials and methods. The study included 57 young women aged 23.1±2.2 years with signs of androgen-dependent dermopathy. The evaluation of the change in the character of menstrual bleeding, the anthropometric parameters (body weight, waist circumference and hips), the therapeutic effect of the drug on the symptoms of androgen-dependent dermopathy, as well as the dynamics of arterial pressure, hemoglobin level, serum iron have been studied. The psycho-emotional state was assessed using the SAN questionnaire (well-being-activity-mood). Results. During 6 months of observation, there was no significant change in the body mass index, waist circumference, and hips, and the drug did not affect the blood pressure numbers. Against the background of taking the drug, there was an increase in the parameters of iron metabolism (hemoglobin content, serum iron). After 3 months of taking the contraceptive with drospirenone, the number of patients with a complaint about the abundance of menstruation decreased more than twofold (from 22.8 to 10.5%), and after 6 months of taking the drug no patient noted the profuse nature of menstruation. Before the start of taking COC with drospirenone, 57.9% of women reported painful menstrual bleeding. Against the background of taking the contraceptive within 3 months, this complaint was stopped in all patients. Sufficient efficacy of treatment of androgen dependent dermopathy in young women with the help of a microdosed drospirenone-containing combined oral contraceptive is estimated from the dermatological acne index. The analysis of the SAN questionnaire made it possible to reveal the improvement in the psychoemotional state of patients on the background of taking the drug. The conclusion. The results obtained proved the effectiveness and safety of the microclinized COC Dimia®. The drug has no significant effect on body weight, blood pressure, provides reliable control of the cycle and a decrease in menstrual bleeding, which results in stabilization of iron metabolism in the body. Dimia® is effective in the treatment of androgen-dependent dermopathy and can be recommended to young women for starting contraception.

Sign in / Sign up

Export Citation Format

Share Document