Effects of Early Sub-Therapeutic Antibiotic Administration and its Subsequent Withdrawal on Body Composition, Gut Microbiota and Metabolite Profiles in a Pig Model

Abstract Background: Antibiotic exposure in early life has shown to be a significant risk factor for later fat accumulation in human. However, whether early sub-therapeutic antibiotic (STA) exposure affects body composition and its mechanisms remains unclear. The present study used a combination of comparative slaughter method, microbiota, and metabolomics measurement to investigate the effects of early STA administration and its subsequent withdrawal on body composition, colonic microbiota and metabolite profiles in a pig model. The piglets were fed the same basal starter diet added with STA (STA) or without STA (CON) for two weeks during the administration period, and then all piglets were switched to the same nursery diet without STA during the withdrawal period until they reached approximately 25 kg body weight. Results: Results showed that STA did not significantly improve piglet growth performance during the administration period and the withdrawal period. Piglets treated with the STA had a lower body water deposition (g/d) during the withdrawal period, and tended to have increased body lipid deposition (g/d) during the withdrawal period and the whole period than CON group. It was found that STA was initially effective in decreasing the abundance of pathogenic bacteria during the administration period, such as Alloprevotella, Bacteroides, Solobacterium, and Sutterella. However, they could not continue the effect during the withdrawal period, leading to the rebound of pathogenic bacteria such as Alloprevotella and the increase of the abundance of other pathogenic bacteria like Oscillibacter. Remarkably, STA treatment decreased the abundance of Blautia that play a potential protective role against obesity either during the administration period or the withdrawal period. Metabolomic analysis indicated that STA mainly altered amino acid metabolism, lipid metabolism, and carbohydrate metabolism during the two periods. Furthermore, Spearman's correlation analysis showed that the gut microbiota was highly correlated with microbial metabolites changes. Conclusion: These results suggest that STA administration may alter tissue deposition through reshaping the gut microbiota and their metabolite profiles.

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Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment

Antimicrobial Resistance and Infection Control ◽

10.1186/s13756-021-00903-0 ◽

2021 ◽

Vol 10 (1) ◽

Author(s):

Isaac Raplee ◽

Lacey Walker ◽

Lei Xu ◽

Anil Surathu ◽

Ashok Chockalingam ◽

...

Keyword(s):

Gut Microbiota ◽

Antibiotic Therapy ◽

Antibiotic Treatment ◽

Nosocomial Infections ◽

Pathogenic Bacteria ◽

Antibiotic Administration ◽

Oral Antibiotic ◽

Opportunistic Pathogens ◽

Taxonomic Assignment ◽

Antibiotic Resistant

Abstract Introduction According to the Centers for Disease Control’s 2015 Hospital Acquired Infection Hospital Prevalence Survey, 1 in 31 hospital patients was infected with at least one nosocomial pathogen while being treated for unrelated issues. Many studies associate antibiotic administration with nosocomial infection occurrence. However, to our knowledge, there is little to no direct evidence of antibiotic administration selecting for nosocomial opportunistic pathogens. Aim This study aims to confirm gut microbiota shifts in an animal model of antibiotic treatment to determine whether antibiotic use favors pathogenic bacteria. Methodology We utilized next-generation sequencing and in-house metagenomic assembly and taxonomic assignment pipelines on the fecal microbiota of a urinary tract infection mouse model with and without antibiotic treatment. Results Antibiotic therapy decreased the number of detectable species of bacteria by at least 20-fold. Furthermore, the gut microbiota of antibiotic treated mice had a significant increase of opportunistic pathogens that have been implicated in nosocomial infections, like Acinetobacter calcoaceticus/baumannii complex, Chlamydia abortus, Bacteroides fragilis, and Bacteroides thetaiotaomicron. Moreover, antibiotic treatment selected for antibiotic resistant gene enriched subpopulations for many of these opportunistic pathogens. Conclusions Oral antibiotic therapy may select for common opportunistic pathogens responsible for nosocomial infections. In this study opportunistic pathogens present after antibiotic therapy harbored more antibiotic resistant genes than populations of opportunistic pathogens before treatment. Our results demonstrate the effects of antibiotic therapy on induced dysbiosis and expansion of opportunistic pathogen populations and antibiotic resistant subpopulations of those pathogens. Follow-up studies with larger samples sizes and potentially controlled clinical investigations should be performed to confirm our findings.

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Effects of Protein Restriction and Subsequent Realimentation on Body Composition, Gut Microbiota and Metabolite Profiles in Weaned Piglets

Animals ◽

10.3390/ani11030686 ◽

2021 ◽

Vol 11 (3) ◽

pp. 686

Author(s):

Lei Hou ◽

Li Wang ◽

Yueqin Qiu ◽

YunXia Xiong ◽

Hao Xiao ◽

...

Keyword(s):

Body Composition ◽

Fatty Acid ◽

Gut Microbiota ◽

Relative Abundance ◽

Protein Restriction ◽

The Body ◽

Fatty Acid Esters ◽

Weaned Piglets ◽

Microbial Composition ◽

Metabolite Profiles

The objective of this study was to evaluate the effects of protein restriction and subsequent protein realimentation on the body composition, gut microbiota and metabolite profiles of piglets. Fifty weaned piglets were randomly assigned to two treatments: a normal protein (NP) group (20% crude protein (CP)) or a low protein (LP) group (16% CP) with five animals per pen and five pens per group. Treatment diets were fed for 14 d during the protein restriction phase, and then all pigs were fed the same nursery diets with a normal CP level (19% CP) during the protein realimentation phase until they reached an average target body weight (BW) of 25 ± 0.15 kg. At day 14 and the end of the experiment, one piglet close to the average BW of each pen was slaughtered to determine body composition, microbial composition and microbial metabolites. Results showed that there was no difference (p > 0.05) in the experimental days to reach target BW between the LP and NP groups. The average daily gain (ADG) and gain:feed ratio (G:F) during the protein restriction phase as well as BW at day 14, were significantly decreased (p < 0.05) in the LP group compared with the NP group. However, there were no significant differences (p > 0.05) during the protein realimentation phase and the overall experiment. Similarly, piglets in the LP group showed a significantly decreased body protein content (p < 0.05) at day 14, but not (p > 0.05) at the end of the experiment. The relative abundance of Parabacteroides, Butyricicoccus, Olsenella, Succinivibrio and Pseudoramibacter were significantly increased (p < 0.05), while the relative abundance of Alloprevotella and Faecalicoccus were significantly decreased (p < 0.05) in the LP group at day 14. At the end of the experiment, the piglets in the LP group showed a higher (p < 0.05) colonic relative abundances of Parabacteroides, unidentified Christensenellaceae and Caproiciproducens, and a lower (p < 0.05) relative abundance of unidentified Prevotellaceae, Haemophilus, Marvinbryantia, Faecalibaculum, Neisseria and Dubosiella than those in the NP group. Metabolomics analyses indicated that tryptophan metabolism and vitamin metabolism were enriched in the LP group at day 14, and glycerophospholipid metabolism and fatty acid esters of hydroxy fatty acid metabolism were enriched at the end of the experiment. Moreover, Spearman’s correlation analysis demonstrated that the microbial composition was highly correlated with changes in colonic metabolites. Collectively, these results indicated that protein restriction and subsequent realimentation lead to compensatory growth and compensatory protein deposition in piglets and contribute to animal intestinal health by altering the gut microbiota and its metabolites.

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Targeting the gut microbiota by Asian and Western dietary constituents: a new avenue for diabetes

Toxicology Research ◽

10.1093/toxres/tfaa065 ◽

2020 ◽

Vol 9 (4) ◽

pp. 569-577 ◽

Cited By ~ 1

Author(s):

Abdul Rahman Conteh ◽

Ruixue Huang

Keyword(s):

Gut Microbiota ◽

Dietary Pattern ◽

Prevention And Control ◽

Significant Risk ◽

Significant Risk Factor ◽

Short Chain Fatty Acids ◽

Western Diet ◽

Essential Components ◽

Clinical Prevention ◽

And Control

Abstract Increasing numerous diabetes annually is a great concern in public health globally. Gut microbiota recently has been suggested to be an emerging organ acting as a critical regulator in diabetes. Notably, gut microbiota is closely affected through an individual’s nutrient intake and dietary pattern. Moreover, the metabolites of diets through gut microbiota are closely associated with the development of diabetes. Increasing evidence has established the association of different dietary pattern with alterations of the gut microbiota profile, in particular, the Asian diet and Western diet are typically as essential components linked to the interactions between gut microbiota and induction of obesity which is a significant risk factor for diabetes. In addition, some bacteria-related therapeutic methods including probiotics, dietary short-chain fatty acids immunotherapy, and gut microbiome transfer would be applied in the clinical prevention and control diabetes. Taken together, based on current published observations, the gut microbiota may serve as regulator or targets by the Asian diet and Western diet, contributing to the prevention or induction of diabetes eventually. In general, in the upcoming future, one of the emerging strategies for the prevention and control of diabetes may modulate gut microbiota through precise dietary strategies.

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Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

10.21203/rs.3.rs-154603/v1 ◽

2021 ◽

Author(s):

Qing Tong ◽

Li-Yong Cui ◽

Jia Bie ◽

Hong-Bin Wang ◽

Jian-Tao Zhang ◽

...

Keyword(s):

Bacterial Community ◽

Gut Microbiota ◽

Pathogenic Bacteria ◽

Alpha Diversity ◽

Amphibian Conservation ◽

Control Group ◽

Antibiotic Administration ◽

Functional Composition ◽

And Control ◽

Rana Dybowskii

Abstract Background: Amphibians frequently receive an antibiotic bath after feedlot placement to control bacterial diseases. The potential collateral effect of these antibiotics on the frog microbiota is largely unknown. Antibiotics are frequently employed to examine the role of the gut microbiota. Existing research relies mainly on oral antibiotics, but knowledge regarding the effects of antibiotics on the gut microbiota through a bath or local antimicrobial therapies is limited. Results: The gut microbiota of gentamicin, recovered, and control Rana dybowskii groups were compared by Illumina high-throughput sequencing, and the functional profiles were analysed using a phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt). Furthermore, the relationship between gut microbiota structures and forecast function compositions was determined. The results showed that the alpha diversity indices were significantly reduced by the gentamicin bath, which significantly changed the composition of the gut microbiota. After 7 days, the gut microbiota was still similar to that during the gentamicin bath. Forty-four indicator species were selected at the genus level, namely, 42 species indicating the control group and 2 species indicating the gentamicin and recovery groups. Potential pathogenic bacteria belonging to Aeromonas, Citrobacter, and Chryseobacterium significantly decreased after the gentamicin bath. The community similarity assays did not show an obvious discrepancy in the functional composition between the gentamicin and control frogs, indicating that the functions of the gut bacterial community were highly redundant. Conclusions: The gentamicin bath significantly reduced the alpha diversity of the gut microbiota of R. dybowskii. Gentamicin significantly changed the structure of the gut microbiota, and the gut microbiotas exhibited weak resilience and did not totally recover after seven days. The gentamicin bath did not change the functional composition of the gut microbiota of R. dybowskii, and there was no significant correlation between the composition of the gut microbiota and the functional composition, illustrating the high intestinal functional redundancy of the frog gut bacterial community. This work offers basic data for upcoming research, including the establishment of the amphibian gut microbiota and local antibiotic administration, and has important implications for aquaculture management and amphibian conservation.

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The Role of Short-Chain Fatty Acids of Gut Microbiota Origin in Hypertension

Frontiers in Microbiology ◽

10.3389/fmicb.2021.730809 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yeshun Wu ◽

Hongqing Xu ◽

Xiaoming Tu ◽

Zhenyan Gao

Keyword(s):

Fatty Acids ◽

Gut Microbiota ◽

Metabolic Regulation ◽

Significant Risk ◽

Significant Risk Factor ◽

Short Chain Fatty Acids ◽

Cerebrovascular Diseases ◽

Short Chain ◽

Hypertension Development ◽

Chain Fatty Acids

Hypertension is a significant risk factor for cardiovascular and cerebrovascular diseases, and its development involves multiple mechanisms. Gut microbiota has been reported to be closely linked to hypertension. Short-chain fatty acids (SCFAs)—the metabolites of gut microbiota—participate in hypertension development through various pathways, including specific receptors, immune system, autonomic nervous system, metabolic regulation and gene transcription. This article reviews the possible mechanisms of SCFAs in regulating blood pressure and the prospects of SCFAs as a target to prevent and treat hypertension.

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Differences in Body Composition among Patientsafter Hemorrhagic and Ischemic Stroke

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17114170 ◽

2020 ◽

Vol 17 (11) ◽

pp. 4170

Author(s):

Jacek Wilczyński ◽

Marta Mierzwa-Molenda ◽

Natalia Habik-Tatarowska

Keyword(s):

Body Composition ◽

Ischemic Stroke ◽

Muscle Mass ◽

Body Mass ◽

Fat Mass ◽

Upper Limb ◽

Significant Risk ◽

Significant Risk Factor ◽

The Body ◽

Fat Free Mass

The aim of the study was to assess differences in the body composition of patients after hemorrhagic and ischemic stroke. There were 74 male participants in the study, of which 13 (18%) experienced hemorrhagic stroke, while 61 (82%) were after ischemic stroke. Significantly (p < 0.05) higher values of body composition variables were noted for ischemic compared to hemorrhagic strokes, and concerned: body mass (BM) (kg), basal metabolic rate (BMR) (kJ), fat-free mass (FFM) (kg), total body water (TBW) (kg), muscle mass (MM) (kg), visceral fat level (VFL), bone mass (BoM) (kg), extracellular water(ECW) (kg),intracellular water (ICW) (kg), trunk fat-free mass (TFFM) (kg) and trunk muscle mass (TMM) (kg)in the paretic upper limb; FFM (kg) and MM (kg) in the non-paretic upper limb; FFM (kg) and MM (kg) in the paretic lower limbas well as FFM (kg) and MM (kg) in the non-paretic lower limb without paresis. Only for the variables fat mass (FM) (kg), body mass index (BMI), metabolic age (MA), trunk fat mass (TFM) (kg), and FM (kg) in the paretic upper limb and FM (kg) in the non-paretic upper limb were there no significant differences. Significant differences in body composition of patients after hemorrhagic and ischemic stroke have been demonstrated. Individuals after ischemic stroke had significantly worse body composition. Incorrect body composition is a significant risk factor, especially of ischemic stroke.

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Physical Illness and Suicide Risk in Rural Residents of Contemporary China

Crisis ◽

10.1027/0227-5910/a000271 ◽

2014 ◽

Vol 35 (5) ◽

pp. 330-337 ◽

Cited By ~ 14

Author(s):

Cun-Xian Jia ◽

Lin-Lin Wang ◽

Ai-Qiang Xu ◽

Ai-Ying Dai ◽

Ping Qin

Keyword(s):

Risk Factor ◽

Family Income ◽

Rural China ◽

Significant Risk ◽

Significant Risk Factor ◽

Health Condition ◽

Physical Illness ◽

Rural Residents ◽

Increased Risk ◽

Study Population

Background: Physical illness is linked with an increased risk of suicide; however, evidence from China is limited. Aims: To assess the influence of physical illness on risk of suicide among rural residents of China, and to examine the differences in the characteristics of people completing suicide with physical illness from those without physical illness. Method: In all, 200 suicide cases and 200 control subjects, 1:1 pair-matched on sex and age, were included from 25 townships of three randomly selected counties in Shandong Province, China. One informant for each suicide or control subject was interviewed to collect data on the physical health condition and psychological and sociodemographic status. Results: The prevalence of physical illness in suicide cases (63.0%) was significantly higher than that in paired controls (41.0%; χ2 = 19.39, p < .001). Compared with suicide cases without physical illness, people who were physically ill and completed suicide were generally older, less educated, had lower family income, and reported a mental disorder less often. Physical illness denoted a significant risk factor for suicide with an associated odds ratio of 3.23 (95% CI: 1.85–5.62) after adjusted for important covariates. The elevated risk of suicide increased progressively with the number of comorbid illnesses. Cancer, stroke, and a group of illnesses comprising dementia, hemiplegia, and encephalatrophy had a particularly strong effect among the commonly reported diagnoses in this study population. Conclusion: Physical illness is an important risk factor for suicide in rural residents of China. Efforts for suicide prevention are needed and should be integrated with national strategies of health care in rural China.

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Risk factors of poor prognosis and impairment of activities of daily living in patients with hemorrhagic gastroduodenal ulcers

BMC Gastroenterology ◽

10.1186/s12876-020-01580-w ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Junya Arai ◽

Jun Kato ◽

Nobuo Toda ◽

Ken Kurokawa ◽

Chikako Shibata ◽

...

Keyword(s):

Risk Factors ◽

Activities Of Daily Living ◽

Daily Living ◽

Poor Prognosis ◽

Significant Risk ◽

Care Facility ◽

Significant Risk Factor ◽

Advanced Age ◽

Gastroduodenal Ulcers ◽

Poor Outcomes

Abstract Background Impairment of activities of daily living (ADL) due to hemorrhagic gastroduodenal ulcers (HGU) has rarely been evaluated. We analyzed the risk factors of poor prognosis, including mortality and impairment of ADL, in patients with HGU. Methods In total, 582 patients diagnosed with HGU were retrospectively analyzed. Admission to a care facility or the need for home adaptations during hospitalization were defined as ADL decline. The clinical factors were evaluated: endoscopic features, need for interventional endoscopic procedures, comorbidities, symptoms, and medications. The risk factors of outcomes were examined with multivariate analysis. Results Advanced age (> 75 years) was a significant predictor of poor prognosis, including impairment of ADL. Additional significant risk factors were renal disease (odds ratio [OR] 3.43; 95% confidence interval [CI] 1.44–8.14) for overall mortality, proton pump inhibitor (PPIs) usage prior to hemorrhage (OR 5.80; 95% CI 2.08–16.2), and heart disease (OR 3.05; 95% CI 1.11–8.43) for the impairment of ADL. Analysis of elderly (> 75 years) subjects alone also revealed that use of PPIs prior to hemorrhage was a significant predictor for the impairment of ADL (OR 8.24; 95% CI 2.36–28.7). Conclusion In addition to advanced age, the presence of comorbidities was a risk of poor outcomes in patients with HGU. PPI use prior to hemorrhage was a significant risk factor for the impairment of ADL, both in overall HGU patients and in elderly patients alone. These findings suggest that the current strategy for PPI use needs reconsideration.

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Neurodevelopmental outcome of healthy term newborn with serum bilirubin >15 mg/dl at one year

Journal of Neonatal-Perinatal Medicine ◽

10.3233/npm-200511 ◽

2020 ◽

pp. 1-6

Author(s):

P. Dubey ◽

J. Shrivastava ◽

B.P. Choubey ◽

A. Agrawal ◽

V. Thakur

Keyword(s):

Serum Bilirubin ◽

Tertiary Care ◽

Neurodevelopmental Outcome ◽

Significant Risk ◽

Central India ◽

Significant Risk Factor ◽

Motor Domain ◽

Medical Emergency ◽

One Year ◽

Group 1

BACKGROUND: Neonatal hyperbilirubinemia is a common medical emergency in early neonatal period. Unconjugated bilirubin is neurotoxic and can lead to lifelong neurological sequelae in survivors. OBJECTIVE: To find out the association between serum bilirubin and neurodevelopmental outcome at 1 year of age using Development Assessment Scale for Indian Infants (DASII). METHODS: A prospective cohort study was conducted in the Department of Pediatrics of a tertiary care institution of Central India between January 2018 and August 2019. Total 108 term healthy neonates, with at least one serum bilirubin value of >15 mg/dl, were included. Subjects were divided into three groups based on the serum bilirubin; group 1: (15–20 mg/dl) –85(78.7%) cases, group 2: (20–25 mg/dl) –17(15.7%), and group 3: (>25 mg/dl) –6(5.5%). Developmental assessment was done using DASII at 3, 6, 9, 12 months of age. RESULTS: Out of 108 cases, 101(93.5%) received phototherapy, and 7(6.5%) received double volume exchange transfusion. Severe delay was observed in 5(4.6%) and mild delay in 2(1.9%) cases in the motor domain of DASII at one year. Severe delay in the motor domain was associated with mean TSB of 27.940±2.89 mg/dl and mild delay with mean TSB of 22.75±1.76 mg/dl (p = 0.001). On cluster analysis, delay was observed in locomotion 1 score in 11(13%) cases (p = 0.003) and manipulation score in 6(7.1%) cases in group 1. CONCLUSION: Increased serum bilirubin was a significant risk factor for the delayed neurodevelopment in babies with neonatal jaundice. Even a moderate level of bilirubin significantly affects the developmental outcome.

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Higher premature atrial complex burden from the Holter examination predicts poor cardiovascular outcome

Scientific Reports ◽

10.1038/s41598-021-91800-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ting-Chun Huang ◽

Po-Tseng Lee ◽

Mu-Shiang Huang ◽

Pei-Fang Su ◽

Ping-Yen Liu

Keyword(s):

Risk Factor ◽

Dose Response ◽

Cox Model ◽

Significant Risk ◽

Significant Risk Factor ◽

Cardiovascular Death ◽

Specific Model ◽

Distribution Model ◽

All Cause Mortality ◽

Quartile Group

AbstractPremature atrial complexes (PACs) have been suggested to increase the risk of adverse events. The distribution of PAC burden and its dose–response effects on all-cause mortality and cardiovascular death had not been elucidated clearly. We analyzed 15,893 patients in a medical referral center from July 1st, 2011, to December 31st, 2018. Multivariate regression driven by ln PAC (beats per 24 h plus 1) or quartiles of PAC burden were examined. Older group had higher PAC burden than younger group (p for trend < 0.001), and both genders shared similar PACs distribution. In Cox model, ln PAC remained an independent risk factor for all-cause mortality (hazard ratio (HR) = 1.09 per ln PAC increase, 95% CI = 1.06‒1.12, p < 0.001). PACs were a significant risk factor in cause-specific model (HR = 1.13, 95% CI = 1.05‒1.22, p = 0.001) or sub-distribution model (HR = 1.12, 95% CI = 1.04‒1.21, p = 0.004). In ordinal PAC model, 4th quartile group had significantly higher risk of all-cause mortality than those in 1st quartile group (HR = 1.47, 95% CI = 1.13‒1.94, p = 0.005), but no difference in cardiovascular death were found in competing risk analysis. In subgroup analysis, the risk of high PAC burden was consistently higher than in low-burden group across pre-specified subgroups. In conclusion, PAC burden has a dose response effect on all-cause mortality and cardiovascular death.

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