scholarly journals Evaluation of microbiological risks in cream as a raw material for buttermaking

Food systems ◽  
2022 ◽  
Vol 4 (4) ◽  
pp. 259-268
Author(s):  
G. M. Sviridenko ◽  
M. B. Zakharova ◽  
N. V. Ivanova

The article presents the research results of studying the influence of various groups of microorganisms — coliform bacteria, lactic acid microorganisms, yeast, and spore bacteria — on the quality and storage capacity of cream used as a raw material for buttermaking. The objects of study were the following: cream as a raw material before and after pasteurization, as well as pasteurized cream seeded with testing cultures of various types of spoilage microorganisms. The samples were stored at temperature conditions of 30 ± 1 °C, 10 ± 1 °C, and 4 ± 2 °C. To evaluate the quality and storage capacity of cream used as a raw material, its microbiological and physicochemical indicators were determined by standardized methods: bacterial number, titratable acidity, indicators of oxidative spoilage of the fat phase. Organoleptic characteristics were evaluated in terms of taste, consistency and appearance. Research results have shown that the greatest microbiological risks during storage of cream used as a raw material are associated with lactococci, coliform bacteria and yeast. Microbiological risks caused by seeding of cream with thermophilic streptococcus, spore bacteria of the genus Bacillus and spore anaerobic microorganisms of the genus Clostridium are less significant, which is associated with the lack of development and metabolism of these groups of microorganisms at storage temperatures of 10 ± 1 °C and 4 ± 2 °C. At the same time, the reason for the rejection of cream contaminated with these testing cultures, at a storage temperature of 4 ± 2 °C, is primarily a decrease in organoleptic indicators, and at a temperature of 10 ± 1 °C — an excess in bacterial number.

2006 ◽  
Vol 52 ◽  
pp. 17-24
Author(s):  
Lela Acevska ◽  
Kristina Mladenovska ◽  
Tanja Petreska Ivanovska ◽  
Maja Jurhar Pavlova ◽  
Milena Petrovska ◽  
...  

Viability of L. casei during fermentation in soymilk and freeze-dried soymilk; effect of cryoprotectant, rehydration and storage temperature The aim of the work was to investigate the behaviour of L. casei and the effect of sorbitol on its viability during fermentation in soymilk drink. Values for pH, ranging from 6.82 to 3.42 in the soymilk drink without sorbitol and from 6.74 to 3.41 in the drink with sorbitol were noted during 72 h of fermentation at 25oC. The corresponding values for titratable acidity ranged from 0.071% to 0.758% and from 0.073% to 0.761%, respectively. Soymilk was found to support the growth of L. casei with improvement in viability for 0.24 log at the end of fermentation when sorbitol was added. Survival of L. casei and the effectiveness of sorbitol in improving viability during freeze-drying, subsequent rehydration and during a 5-week period of storage under different temperatures were also investigated. After freeze-drying, L. casei exhibited a survival percent of approximately 46%. Sorbitol improved the viability of L. casei by 0.51 log immediately after freeze-drying and by 1.30 log and 0.47 log during five weeks of storage at 25oC and 4oC, respectively. Further study revealed that the freeze-dried fermented soymilk rehydrated at 45oC was optimum for the recovery of L. casei with improvement in recovery for 0.68 log when sorbitol was added. A higher percent of survival was noted when the dried soymilk was stored at 4oC than at 25oC with improved viability at the end of 5 weeks storage for approximately 6 log for drinks with and without sorbitol. Fermented dried soymilk with sorbitol afforded significant tolerance of L. casei to acid stress. Generally, a stable probiotic diary product was prepared in which the concentration of L. casei remained above therapeutic level of 107 cfu/ml.


2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Nurul Husniyati Listyana ◽  
Rahma Widyastuti ◽  
Widyantoro Widyantoro

ABSTRACT Meniran (Phyllanthus niruri L.) is a wild plant that used extensively as a raw material for traditional medicine but has not been cultivated yet. Meniran cultivation requires the availability of quality seeds and seedlings to produce the high productivity. The ability of seeds to germinate (seed viability) after the storage period is influenced by several factors both internal and external, including seed water content, genetic traits, initial viability and packaging material, storage temperature and humidity, microorganisms, and human factors.. The study aims to determine the effect of storage containers, storage temperature and time of storage on the power of meniran seed germination. The study was conducted in March-April 2018 at the B2P2TO2T Seed Laboratory. The research design was Factorial Completely Randomized Design with 3 factors and 3 replications. The first factor was a storage container consisting of plastic, paper envelopes and aluminum foil. Second factor was a storage temperature consisting of 0 ° C, -20 ° C and -50 ° C.  The third factor was storage time which consists of 2 weeks, 4 weeks, 6 weeks, 8 weeks and 10 weeks. The results showed that the storage container and storage time had a significantly different effect on meniran seed germination. The storage time does not have a significant effect on germination. Together, the storage container, storage temperature and storage time do not have a significant effect on meniran seed germination. The interaction between the storage temperature and the storage time and the interaction between the storage container and the storage time give significantly different effects on germination. ABSTRAK Meniran (Phyllanthus niruri L.) merupakan tanaman liar yang simplisianya banyak dibutuhkan sebagai bahan baku obat tradisional namun belum dibudidayakan. Budidaya tanaman meniran memerlukan ketersediaan benih dan bibit yang bermutu untuk menghasilkan produktivitas yang tinggi. Kemampuan benih untuk berkecambah (viabilitas benih) setelah masa penyimpanan dipengaruhi oleh beberapa faktor baik internal maupun eksternal, meliputi kadar air benih, sifat genetik, viabilitas awal serta bahan pengemas, suhu dan kelembaban ruang simpan, mikroorganisme, serta faktor manusia. Penelitian bertujuan untuk mengetahui pengaruh wadah simpan, suhu simpan dan waktu simpan terhadap daya perkecambahan benih meniran. Penelitian dilaksanakan pada bulan Maret-April 2018 di Laboratorium Benih B2P2TO2T. Desain penelitian yang digunakan yaitu Rancangan Acak Lengkap Faktorial dengan 3 faktor dan 3 ulangan. Faktor 1 yaitu wadah penyimpanan yang terdiri dari plastik, kertas amplop dan aluminium foil. Faktor 2 yaitu suhu simpan yang terdiri dari 0°C, -20°C dan -50°C. Faktor 3 yaitu waktu penyimpanan yang terdiri dari 2 minggu, 4 minggu, 6 minggu, 8 minggu dan 10 minggu. Hasil penelitian menunjukkan wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran.  Suhu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan. Secara bersama-sama wadah simpan, suhu simpan dan waktu simpan tidak memberikan pengaruh yang berbeda signifikan terhadap perkecambahan benih meniran. Interaksi antara suhu simpan dan waktu simpan serta interaksi antara wadah simpan dan waktu simpan memberikan pengaruh yang berbeda signifikan terhadap perkecambahan.  


DYNA ◽  
2018 ◽  
Vol 85 (207) ◽  
pp. 84-92 ◽  
Author(s):  
Elizabeth Cristina Acevedo-Martínez ◽  
Carolina Gutiérrez Cortés ◽  
Maribel García Mahecha ◽  
Consuelo Díaz Moreno

The interest about probiotic bacteria supplementation in fruit beverages has been increased recently in order to develop functional beverages without lactose. That is a technological challenge since pH and storage temperature can affect their viability. The aim of this study was to evaluate the viability of three commercial probiotic strains (L. paracasei, L. casei and L. rhamnosus) in mango beverage stored at 4°C. This research was developed in three stages: the assessment of the acid strain tolerance, the selection of a prebiotic as cellular protectant against environmental stress, the evaluation of physiochemical and sensory stability during storage. L. casei was chosen as the most stable strain with FOS at 5% as a prebiotic substance that stimulated its growth. At the final stage, it was found that L. casei produced significant changes in pH and titratable acidity in mango beverages after one week at 4°C, which was also detected by consumers with good sensory acceptance


Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3430
Author(s):  
Thi Minh Phuong Ngo ◽  
Thanh Hoi Nguyen ◽  
Thi Mong Quyen Dang ◽  
Thi Van Thanh Do ◽  
Alissara Reungsang ◽  
...  

The aim of extending shelf-life and maintaining quality is one of the major issues regarding mango fruit preservation. The quality of mango fruits is greatly affected by postharvest factors, especially temperature and fruit treatment. In this study, the effect of coating and storage temperature on the characteristics of mango fruits was investigated. The mango fruits were immersed in different concentrations (1.5%, 2.0%, and 2.5%) of pectin/nanochitosan dispersion (with ratios of pectin:nanochitosan 50:50), and (0.75%, 1% and 1.25%) of nanochitosan dispersion and stored at 17, 25, and 32 °C for 24 days. Changes in fruit, including weight loss, firmness, color, chemical composition (such as the total soluble solids concentration (TSS)), total sugar, reducing sugar, titratable acidity (TA), and vitamin C were periodically recorded. The results indicated that the pectin/nanochitosan coating significantly prevented reductions in the fruit weight, firmness, TSS, TA, and vitamin C content. Additionally, pectin/nanochitosan at a low temperature (17 °C) had a greater positive effect on fruit shelf-life and weight maintenance than 25 and 32 °C. The coated mango fruits maintained good quality for 24 days at 17 °C, while coated fruits stored at 25 °C and 32 °C, as well as uncoated ones stored at 17 °C, were destroyed after two weeks. At the maximum storage time evaluated, the coating formulations containing pectin and nanochitosan exhibited microbial counts below the storage life limit of 106 CFU/g of fruit. In general, the results showed that the pectin/nanochitosan coating (2%) with a storage temperature of 17 °C is the most effective strategy for improving quality and extending the shelf-life of mango fruits.


Author(s):  
T. A. Velesyk ◽  
R. M. Sachuk ◽  
B. V. Gutyj ◽  
A. S. Kushniruk ◽  
V. O. Pepko ◽  
...  

The most common problem among ready-made foods is the adulteration of butter, which is replaced by margarine or vegetable spreads. Milk and other dairy products made from raw milk are not left out. The production and sale of low-quality, counterfeit products poses a direct threat to human health and affects the competitive environment among producers. As a result of research, it should be noted that the raw milk market is not fully saturated, so improving the quality of cream production and processing of milk products, namely butter, competition for suppliers, microbiological improvements, as well as improvements to plants and systems – remains relevant. During the analysis, some physicochemical parameters of butter were studied, namely: acidity of the fat phase, mass fraction of moisture and fat. The acidity index was within the norm, although it differed in all samples: the lowest was found in sample № 1 (1.1 °K), and the highest was in sample № 2 (1.6 °K). As for such an indicator as the content of table salt and the content of fat and pH of plasma, the oils Sweet cream “Selyanske”, 72,6 % TM “Svoya Liniya”, Sweet cream, 73.0 % LLC “Eney”, “Poltavske”, 62.5 % LLC “Techmolprom” do not meet the requirements of DSTU 4399:2005. The mass fraction of fat in butter brands PJSC “Dubnomoloko” and “Pryvat-Fud” – was lower than indicated on the package, by 1.4 % and 2.6 %, respectively, and brands “Svoya Liniya”, LLC “Eney” and Techmolprom LLC on the contrary – by 18.0 %, 18.4 % and 12.5 % more. According to the results of microbiological studies, it was found that the number of mesophilic aerobic and facultatively anaerobic microorganisms, yeasts, fungi and bacteria of the Escherichia coli group in the studied samples of butter of all brands does not exceed the permissible norms. This fact testifies to the relative “purity” of the raw material from which the butter was made, as well as to the observance of veterinary and sanitary and hygienic norms during the production and storage of butter. Summing up the research, it should be noted that, despite the identified physical and chemical shortcomings of all samples of butter, preference should be given to sour cream butter “Selyanske”, 73.0 % of PJSC “Dubnomoloko” and “Selyanske” sweet cream, 73.0 % “Privat- Fud”, as none of them exceeded such indicators as fat content and plasma pH of oil, which may indicate the absence of impurities in vegetable fats in this product.


2018 ◽  
Vol 2 ◽  
pp. 62-67
Author(s):  
Dmytro Odarchenko ◽  
Andrey Odarchenko ◽  
Evgenia Sokolova ◽  
Vladimir Mikhailik

A semi-product for cooking a smoothie drink was chosen as a subject of the study. It included strawberries, dried apples and oat flocks. A sample was frozen in a low-temperature calorimeter to –20 °С. It was established, that freezing at–20±2 °С favors the full conservation of a sample and storage during 270 days. The results of the organoleptic evaluation of the semi-product before freezing and after refrigeratory storage during 270 demonstrated that the product is characterized by a high quality. Microbiological parameters of the fresh and frozen semi-product were studied for a quantity of mesophilic aerobic and facultative-anaerobic microorganisms (QMAFMAnM). And also for bacteria of the colibacillus group (BGCB), pathogenic microorganisms of bacteria of Salmonella genus, molds and yeast. The gram-negative microflora – bacteria of the colibacillus group, pathogenic microorganisms including bacteria of Salmonella genus, were not observed in samples of frozen semi-products. The studies demonstrated freezing advantages from both aspects of minimization of losses of valuable biocomponents of the raw material and of achieving high organoleptic parameters of products after freezing and defrosting. The observance of optimal conditions of freezing excludes the necessity of the additional use of artificial preservatives, provides high-quality products and safety that corresponds to all principles of healthy nutrition.


2021 ◽  
Vol 41 (2) ◽  
pp. e82530
Author(s):  
Javier Giovanni Alvarez Herrera ◽  
Yuli Alexandra Deaquiz ◽  
Ximena Rozo-Romero

Plums are classified as a climacteric fruit with a high respiration rate between the end of their development and the start of the ripening process, thus making it necessary to apply techniques to preserve the organoleptic characteristics required for the product to have good market acceptance. The aim of this study was to evaluate the effect of harvest maturity (states 2, 3, 4, and 5) and storage temperature (2, 4, and 18 °C) on the organoleptic and physicochemical quality and postharvest storage duration of ‘Horvin’ plums. The experimental design was completely randomized with a 4 x 3 factorial arrangement. The first factor corresponded to the stage of maturity, and the second one to refrigeration temperatures, for a total of 12 treatments. Every three or four days, fruit peel color, accumulated mass loss, respiratory intensity, firmness, pH, total titratable acidity, and total soluble solids were measured every during storage. Measurements were taken until the fruits lost their organoleptic quality. For most of the measured variables, the fruits stored at 2 °C and harvested in maturity stage 5 maintained the best postharvest quality during storage. The fruits without refrigeration only reached 10 days, while the fruits stored at 4 °C and 2 °C maintained quality of consumption for 24 and 31 days, respectively. Maturity stage 5 presented the highest values of accumulated mass loss, pH, total soluble solids, color index, maturity index, and respiratory intensity.


2004 ◽  
Vol 26 (1) ◽  
pp. 168-170 ◽  
Author(s):  
Maria Inês Sucupira Maciel ◽  
Vera Lúcia Arroxelas Galvão de Lima ◽  
Eufrásio Souza dos Santos ◽  
Marilene da Silva Lima

The objective of this work was to compare the effects of four different concentrations of cassava starch film and storage temperature on shelf life and the quality of a genotype of acerola from the Germplasm Active Bank (GAB) of Federal Rural University of Pernambuco, Brazil. Sound orange-reddish acerola fruits were washed with a chlorine solution (100 mg.L-1 active chlorine) and randomly distributed into different lots. The fruits were dipped for 3 min in a cassava starch suspension with concentrations of 1, 2, 3 and 4% (w/v) and the control without coating, stored at 10ºC (85% RH) and 22ºC (85% RH). The total soluble solids (TSS), pH, titratable acidity (TA) and ascorbic acid (AA) were determined at harvest and regular interval during storage. The use of cassava biofilm at 1% on acerola fruits maintained the highest ascorbic acid content and the temperature of 10ºC extended storage life. The fruits coated with 1 and 2% biofilm could be stored for a period up to 15 days at 10ºC, with acceptable quality characteristics.


HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 164-169 ◽  
Author(s):  
Christopher B. Watkins ◽  
Mustafa Erkan ◽  
Jacqueline F. Nock ◽  
Kevin A. Iungerman ◽  
Randolph M. Beaudry ◽  
...  

`Honeycrisp' is a new apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] cultivar that has been planted extensively in North America, but the storage disorders soggy breakdown and soft scald have resulted in major fruit losses. The effects of harvest date and storage temperature on fruit quality and susceptibility of fruit to these disorders have been investigated in Michigan, New York, and Maine. Internal ethylene concentrations were variable over a wide range of harvest dates, and a rapid increase in autocatalytic ethylene production was not always apparent. The starch pattern index, soluble solids content, titratable acidity and firmness also appear to have limited use as harvest indices. Development of soggy breakdown and soft scald is associated with later harvest dates and storage of fruit at temperatures of 0 to 0.5 °C compared with higher storage temperatures. It is recommended that `Honeycrisp' be stored at 3 °C, although storage disorders still can occur at this temperature if fruit are harvested late. In addition, greasiness development may be worse at higher storage temperatures.


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