scholarly journals Evaluation of in vitro antimicrobial effects of Azadirachta indica (Neem) leaves extracts against selected pathogens

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Waleed Fasher Bory ◽  
Mohammed Hammad ◽  
Alkhair Idris
Keyword(s):  
Azadirachta Indica ◽  
Antimicrobial Effects ◽  
Neem Leaves

scholarly journals UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21

2017 ◽  
Vol 7 (1) ◽  
pp. 48
Author(s):  
Qurni Restiani ◽  
Mandojo Rukmo ◽  
Devi Eka Juniarti
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Azadirachta Indica ◽  
Root Canal ◽  
In Vitro Study ◽  
Cytotoxicity Test ◽  
Root Canal Irrigants ◽  
Neem Leaves ◽  
Alternative Material

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

Methanolic Extraction, Formulation and Evaluation of Herbal Transdermal Patches of Azadirachta indica A. Juss

2021 ◽  
pp. 3709-3715
Author(s):  
D. Pooja Reddy ◽  
S.B. Bhanja ◽  
Ashwini K Chauhan ◽  
B. Kranthi Kumar ◽  
Dibya Sunder Panda ◽  
...  
Keyword(s):  
Drug Release ◽  
Azadirachta Indica ◽  
Ex Vivo ◽  
Fickian Diffusion ◽  
Transdermal Patches ◽  
Ex Vivo Permeation ◽  
Neem Leaves ◽  
In Vitro Diffusion ◽  
Bacterial Screening

At present, synthetic drugs form a major line of treatment in the management of many diseases and currently available as transdermal patches. Traditional medicine system is centuries old practice and again gaining importance. Hence, herbal products can be used to treat many diseases as transdermal patches. Neem leaves has antibacterial properties and can be used for controlling air borne bacterial contamination. Azadirachta Indica. A. Juss (neem) very useful traditional plant. The present study was carried out to extract, formulate and evaluate a transdermal patches containing Azadirachta indica A. Juss The total four Transdermal patches were prepared by solvent casting method and evaluated for physicochemical characteristics such as weight variation, thickness, drug content uniformity, folding endurance, In-vitro diffusion, ex-vivo permeation studies and anti-Bacterial screening test. The infrared spectroscopy showed that there was no incompatibility between drug and polymer. The In-vitro diffusion studies of Transdermal patches of Neem showed percentage of drug release from 65.2% to 92.06% at the end of 21hrs. The ex-vivo permeation study was carried out for optimized formulation (M2) using goat abdomen skin as barrier and showed percentage drug release 89.6% at the end of 21hrs. Release kinetics data showed that all the formulations followed zero order kinetics with non-Fickian diffusion mechanism. The anti-bacterial screening study showed good anti-bacterial activity against Bascillus subtilis and Pseudomonas aeruginosa and zone of Inhibition (ZOI) was compared against standard antibiotic drugs i.e. Penicillin and Streptomycin.

scholarly journals Preclinical Evaluation of the Supercritical Extract of Azadirachta Indica (Neem) Leaves In Vitro and In Vivo on Inhibition of Prostate Cancer Tumor Growth

2014 ◽  
Vol 13 (5) ◽  
pp. 1067-1077 ◽  
Author(s):  
Qiang Wu ◽  
Manish Kohli ◽  
H. Robert. Bergen ◽  
John C. Cheville ◽  
R. Jeffrey Karnes ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Tumor Growth ◽  
Azadirachta Indica ◽  
Preclinical Evaluation ◽  
Neem Leaves ◽  
Cancer Tumor

Analysis of Chemical Composition by GC-MS and in-vitro Antidiabetic Activity of Azadirachta indica Leaves Collected from Erbil, Iraq

2020 ◽  
Vol 07 ◽  
Author(s):  
Javed Ahamad ◽  
Subasini Uthirapathy ◽  
Esra T. Anwer ◽  
Muath Sh. Mohammed Ameen ◽  
Faiq H.S. Hussain
Keyword(s):  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Antidiabetic Activity ◽  
Chemical Components ◽  
Neem Extract ◽  
Neem Leaves ◽  
Ic50 Values

Background: Azadirachta indica (Neem) is an important medicinal plant that is traditionally known for its insecticidal, and antimicrobial properties. Objective: The objective of present study was to evaluate the in-vitro antidiabetic activity and characterize the chemical constituents of Neem extract. Materials and Methods: The chemical constituents in ethyl acetate extract of the Neem leaves was determined by GC-MS and the in-vitro antidiabetic activity of Neem extract was evaluated via inhibition of α-amylase and α-glucosidase enzymes. Results: The ethyl acetate extract of Neem yielded 49 chemical compounds and these constituents amounting to 97.07% of total Neem leaf extract composition. The major chemical components of the Neem were octadecanoic acid (7.17%), propyl tetracosyl ether (5.35%), 1-decanol, 2-octyl (5.01%), hexadecane (4.70%), tricosane (4.73%), octadecane, 1-chloro (4.65%), and linolenic acid (4.35%). The Neem extract produces dose dependent-inhibition of α-amylase and α-glucosidase enzymes ranging from 21.67±2.81 to 75.82±3.44% and 17.04±4.43 to 58.44±1.21% for concentration 31.25 to 1000 µg/mL, respectively. The IC50 values for Neem extract and acarbose were 165.53±2.75 and 91.04±2.16 µg/mL, respectively against the α-amylase enzyme. The IC50 values for Neem extract and acarbose were 210.08±2.16 and 120.05±2.16 µg/mL, respectively against the α-glucosidase enzyme. Conclusion: The study findings suggested that Neem may be used as drug candidate in the management of post prandial hyperglycemia.

scholarly journals IN VITRO COMPARISON OF THE ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF AZADIRACHTA INDICA LEAVES WITH GENTAMYCIN, AMPICILLIN, NITROFURANTOIN, AND COTRIMOXAZOLE ON BACTERIAL PATHOGENS ISOLATED FROM URINARY TRACT INFECTION PATIENTS

2017 ◽  
Vol 10 (8) ◽  
pp. 72 ◽  
Author(s):  
Meenakshi Jindal ◽  
Chauhan S
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Neem Leaves ◽  
Mean Diameter ◽  
The Mean ◽  
In Vitro Comparison

  Objective: In vitro comparison of the antibacterial activity of ethanolic extract of Azadirachta indica (neem) leaves with gentamycin, ampicillin, nitrofurantoin, and cotrimoxazole in bacterial pathogens isolated from urinary tract infection (UTI) patients.Methods: Ethanolic extract of neem leaves was prepared by the standard method. The antimicrobial activity against bacteria isolated from UTI patients was determined by agar well diffusion method and then mean zone of inhibition of neem extract was compared with a mean zone of inhibition of gentamycin, ampicillin, nitrofurantoin, and cotrimoxazole.Results: Among 200 samples which were included in the study, bacteria isolated were Escherichia coli (60%), Klebsiella pneumoniae (15%), Pseudomonas aeruginosa (11%), Enterococcus faecalis (5%), Proteus mirabilis (3%), and Staphylococcus aureus (6%). The result obtained was statistically analyzed by unpaired t-test. The difference in the mean diameter of the zone of inhibition between ethanolic extract of Azadirachta indica (neem) leaves and nitrofurantoin was statistically highly significant for K. pneumoniae (p<0.0001) and P. mirabilis (p=0.01) and insignificant for other bacteria. On comparing, the mean diameter of the zone of inhibition of ethanolic extract of A. indica (neem) leaves with ampicillin and cotrimoxazole, it was found to be statistically highly significant for all bacteria E. coli (p<0.0001), K. pneumoniae (p<0.0001), P. aeruginosa (p<0.0001) E. faecalis (p<0.001), and S. aureus (p<0.0001) expect P. mirabilis. The mean diameter of the zone of inhibition of ethanolic extract of A. indica (neem) leaves when compared with gentamycin was statistically highly significant for all the bacteria.Conclusion: The ethanolic extract of A. indica leaves has antibacterial activity. Antibacterial activity of ethanolic extract of A. indica leaves is comparable to nitrofurantoin for bacteria E. coli, P. aeruginosa, E. faecalis, and S. aureus. Ethanolic extract of A. indica leaves has similar antibacterial activity as ampicillin and cotrimoxazole for bacteria P. mirabilis. The antibacterial activity of gentamycin is less as compared to an ethanolic extract of A. indica leaves for all the bacteria.

Optimization of the Microwave-Assisted Extraction of Azadirachta indica (Neem) Leaves Using NMR-based Metabolic Fingerprinting

2015 ◽  
Vol 11 (3) ◽  
pp. 142-145 ◽  
Author(s):  
Simone Carradori ◽  
Luisa Mannina ◽  
Federica De Cosmi ◽  
Tamara Beccarini ◽  
Daniela Secci ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Metabolic Fingerprinting ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Neem Leaves

scholarly journals Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 708
Author(s):  
Radek Sleha ◽  
Vera Radochova ◽  
Jiri Malis ◽  
Alexander Mikyska ◽  
Milan Houska ◽  
...  
Keyword(s):  
Wound Healing ◽  
Porcine Model ◽  
Therapeutic Effects ◽  
Methicillin Resistant ◽  
Antimicrobial Effects ◽  
Therapeutic Modalities ◽  
Positive Effects ◽  
Inflammatory Parameters

Staphylococcus (S.) aureus is an important causative agent of wound infections with increasing incidence in the past decades. Specifically, the emergence of methicillin-resistant S. aureus (MRSA) causes serious problems, especially in nosocomial infections. Therefore, there is an urgent need to develop of alternative or supportive antimicrobial therapeutic modalities to meet these challenges. Purified compounds from hops have previously shown promising antimicrobial effects against MRSA isolates in vitro. In this study, purified beta-acids from hops were tested for their potential antimicrobial and healing properties using a porcine model of wounds infected by MRSA. The results show highly significant antimicrobial effects of the active substance in both the powder and Ambiderman-based application forms compared to both no-treatment control and treatment with Framycoin. Moreover, the macroscopic evaluation of the wounds during the treatment using the standardized Wound Healing Continuum indicated positive effects of the beta-acids on the overall wound healing. This is further supported by the microscopic data, which showed a clear improvement of the inflammatory parameters in the wounds treated by beta-acids. Thus, using the porcine model, we demonstrate significant therapeutic effects of hops compounds in the management of wounds infected by MRSA. Beta-acids from hops, therefore, represent a suitable candidate for the treatment of non-responsive nosocomial tissue infections by MRSA.

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