scholarly journals Removal of total nitrogen from wastewater by a combination of Chlorella sp. and audible sound

2021 ◽  
Author(s):  
Thanh-Luu Pham ◽  
Uyen Phuong Tran ◽  
Nghia Hiep Bui ◽  
Thuy Thi Ngoc Bach ◽  
Binh Van Tran ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Total Nitrogen ◽  
Classical Music ◽  
Critical Role ◽  
Coefficient Of Determination ◽  
P Value ◽  
Chlorella Sp ◽  
Screening Experiments ◽  
Sound Control ◽  
Audible Sound

Abstract In developing countries, nitrogen in the traditional market wastewater is a critical environmental problem. In this study, the microalga Chlorella sp., which was isolated from wastewater, was used to remove the total nitrogen (TN) from conventional market wastewater in combination with audible sound (Vietnamese classical music). In addition, effects of sound exposure on removal efficiency at different initial cell densities were analyzed. Results revealed that music sound control demonstrates potential to improve the removal efficiency. TN removal efficiencies of 96%, 69.5%, and 4.3% were observed for treatments with Chlorella sp./audible sound, Chlorella sp., and without Chlorella sp., respectively. The significance of probability value (p-value) (<0.05) on the paired sample t-test confirmed the critical role of audible sound and Chlorella sp. density on the TN removal in screening experiments. The predicted optimal conditions for TN removal were as follows: a Chlorella sp. density of 4%, an audible sound of 52.5 dB, and a cultivation time of 4.6 days. Results based on statistical analysis revealed that the quadratic models for TN removal are significant at a low p-value (<0.05) and a high predicted coefficient of determination (R2 = 0.9452) value. The obtained statistical results also indicated that most of the variables are significant for the abatement of TN from market wastewater using Chlorella sp.

scholarly journals Satellite-Derived Protein Concentration of Phytoplankton in the Southwestern East/Japan Sea

2021 ◽  
Vol 9 (2) ◽  
pp. 189
Author(s):  
Hyeonji Bae ◽  
Dabin Lee ◽  
Jae Joong Kang ◽  
Jae Hyung Lee ◽  
Naeun Jo ◽  
...  
Keyword(s):  
Protein Concentration ◽  
Japan Sea ◽  
Biological Properties ◽  
Measured Data ◽  
Trophic Levels ◽  
Coefficient Of Determination ◽  
P Value ◽  
Chl A ◽  
Major Factors

The cellular macromolecular contents and energy value of phytoplankton as primary food source determine the growth of higher trophic levels, affecting the balance and sustainability of oceanic food webs. Especially, proteins are more directly linked with basic functions of phytoplankton biosynthesis and cell division and transferred through the food chains. In recent years, the East/Japan Sea (EJS) has been changed dramatically in environmental conditions, such as physical and chemical characteristics, as well as biological properties. Therefore, developing an algorithm to estimate the protein concentration of phytoplankton and monitor their spatiotemporal variations on a broad scale would be invaluable. To derive the protein concentration of phytoplankton in EJS, the new regional algorithm was developed by using multiple linear regression analyses based on field-measured data which were obtained from 2012 to 2018 in the southwestern EJS. The major factors for the protein concentration were identified as chlorophyll-a (Chl-a) and sea surface nitrate (SSN) in the southwestern EJS. The coefficient of determination (r2) between field-measured and algorithm-derived protein concentrations was 0.55, which is rather low but reliable. The satellite-derived estimation generally follows the 1:1 line with the field-measured data, with Pearson’s correlation coefficient, which was 0.40 (p-value < 0.01, n = 135). No remarkable trend in the long-term annual protein concentration of phytoplankton was found in the study area during our observation period. However, some seasonal difference was observed in winter protein concentration between the 2003–2005 and 2017–2019 periods. The algorithm is developed for the regional East/Japan Sea (EJS) and could contribute to long-term monitoring for climate-associated ecosystem changes. For a better understanding of spatiotemporal variation in the protein concentration of phytoplankton in the EJS, this algorithm should be further improved with continuous field surveys.

Abstract P392: The Effect Of Cell Sex On Magnetic Nanoparticle Uptake Of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Morteza Mahmoudi ◽  
Vahid Serpooshan ◽  
Phillip C Yang ◽  
Mahyar Heydarpour
Keyword(s):  
Heart Failure ◽  
Actin Filaments ◽  
Electromechanical Coupling ◽  
Critical Role ◽  
Induced Pluripotent Stem Cell ◽  
Patient Specific ◽  
P Value ◽  
Male And Female ◽  
Cell Therapies ◽  
Human Cardiomyocytes

Introduction: It is well understood that the occurrence, progress, and treatment of heart failure, which is a leading cause of death worldwide, is sex-specific. Over the past decade, the majority of efforts in myocardial regeneration have been centered on cell-based cardiac repair. A promising cell source for these efforts is patient-specific human cardiomyocytes (CMs) differentiated from human inducible pluripotent stem cells (hiPSCs). However, successful use of hiPSC-CMs faces a major limitation, the poor engraftment and electromechanical coupling of transplanted cells with the host myocardial tissue. Magnetic nanoparticles (NPs) demonstrate great potential to address this challenge for treating heart failure via cell therapies. In particular, superparamagnetic iron oxide NPs (SPIONs) have been used to label hiPSC-CMs and, with the aid of external magnetic field, improve their engraftment and electromechanical coupling in the heart tissue. However, the critical role of cell sex in the uptake and labeling efficacy of NPs has not been evaluated. Hypothesis: Significant differences in the molecular and structural (e.g., actin structures and distribution) characteristics of male and female hiPSC-CMs affect their labeling efficacy with SPIONs. Methods and Results: To test our hypothesis, we first performed RNA-Seq analysis on three male and three female (healthy) hiPSC-CM lines. The normalized outcomes were analyzed by edgeR package. We next calculated gene-expression differential between male and female CMs. The results revealed 58 genes with significant differences between the male and female cells (p-value < 0.01). The highest observed sex-specific variation in genes was related to tophit gene (MEG3: logFC = 7.32, P-value = 5.63e -06 ), which is the maternally expressed imprinted gene with a great role in cardiac angiogenesis. Among the identified genes, a number of those were related to the cellular cytoskeletal structures including actin. We probed possible structural differences between actin filaments organization and distribution of male and female hiPSC-CMs using the stochastic optical reconstruction microscopy (STORM) technique. The results demonstrated substantial differences in organization, distribution, and morphology of actin filaments between male and female CMs. Incubation of SPIONs with male and female hiPSC-CMs revealed higher uptake of NPs (~ 3 folds) in female cells as compared to the male cells. The significant differences in the uptake of SPIONs by male vs. female cells could be attributed to the distinct organization, distribution, and morphology of actin in male vs. female cells. Conclusions: Our results indicate that male and female hiPSCs-CMs respond differently to the labeling SPIONs.

scholarly journals Correlates of Knowledge of HIV Transmission Among Incident Cases of HIV in a Cohort of Injection Drug Users Receiving Harm Reduction Services at Karachi, Pakistan

2014 ◽  
Vol 16 (3) ◽  
pp. 286-289 ◽  
Author(s):  
Rab Nawaz Samo ◽  
Arshad Altaf ◽  
Sharaf Ali Shah
Keyword(s):  
Harm Reduction ◽  
Injection Drug Users ◽  
Drug Users ◽  
Hiv Transmission ◽  
Critical Role ◽  
Unprotected Sex ◽  
Injection Drug ◽  
P Value ◽  
High Risk Population ◽  
Incident Cases

Background: Knowledge of risk factors for HIV transmission in high-risk population plays a critical role in averting the risk of HIV transmission. In Pakistan, injection drug users (IDUs) constitute the core risk group of HIV prevalence, where the epidemic has transitioned to a “concentrated level.” Still nothing is known about the role of knowledge in HIV transmission and HIV sero-conversion among IDUs in Pakistan. Methods: From 2009 to 2011, a nested case–control study was conducted in a cohort of 636 IDUs receiving harm reduction services in the mega city of Karachi. Results: In multivariable regression analysis, 3 factors, namely HIV does not spread through unprotected sex (adjusted odds ratio [AOR]: 3.1, 95% confidence interval [CI] 1.39-6.90, P value .01), HIV does not transmit by sharing syringes (AOR: 3.5, 95% CI 1.97-6.40, P value <.00), and the risk of HIV cannot be minimized by using new syringe every time (AOR: 2.0, 95% CI 1.16-3.60, P value .01), were significantly associated with the incident cases of HIV. Conclusion: The study findings suggest the association between knowledge of HIV transmission and HIV sero-incident cases.

scholarly journals Hypoxic Status Is Associated With The Intensity Of Hypoxia Inducible Factor (Hif)-1α Expression In A Premature Placenta

2021 ◽  
Vol 17 (1) ◽  
pp. 1-11
Author(s):  
Kartini Edwin
Keyword(s):  
Confidence Interval ◽  
Premature Birth ◽  
Odds Ratio ◽  
Critical Role ◽  
Hypoxia Inducible Factor ◽  
Biological Materials ◽  
P Value ◽  
Live Births ◽  
Infant Morbidity ◽  
The Relationship

Prematurity refers to live births before 37 weeks of gestation and associated with infant morbidity/mortality. Activation of HIF during the final pregnancy phase is believed to play a critical role in the pathogenesis of premature birth and other pregnancy disorders. This study aimed to analyze the relationship between hypoxicstatus and the intensity of HIF-1α expression in a premature placenta.Stored biological materials premature placenta (paraffin blocks) was used in this study. Thirtyone samples of placental hypoxia (H) and 28 samples of premature placental non-hypoxia (N) as controls, were selected non-random consecutively. Subsequently, immunohistochemistry was performed to analyze HIF-1α expression. TheChi-square testwas used to analyze the data and a p-value <0.05 was considered statistically significant.Moderate to strong intensity of HIF-1α expressionwas observed in 58% of hypoxic placenta samples, whereas most of non-hypoxic placental samples(86%) did not expressed or expressed weaklyHIF-1α.There was a significant correlation between the intensity of HIF-1α expression and placental hypoxia (p <0.05) and Odds Ratio (OR) value was 8.31 with a 95% confidence interval (2.32-29.77). The conclusion shows that hypoxic status is associated with intensity of hypoxia inducible factor (HIF)-1α expression in a premature placenta.

scholarly journals Laju Nitrifikasi pada Bioremediasi Air Limbah Organik Menggunakan Chlorella sp. dan Bakteri Nitrifikasi-Denitrifikasi

2021 ◽  
Vol 11 (2) ◽  
pp. 309-323
Author(s):  
Arya Ksatria Fernanda Hendrawan ◽  
Norma Afiati ◽  
Arif Rahman
Keyword(s):  
P Value ◽  
Chlorella Sp

Pencemaran nitrogen secara global begitu meningkat akibat pembuangan air limbah organik bersumber dari aktivitas manusia yang mencemari lingkungan perairan. Peningkatan senyawa nitrogen secara terus menerus dan dalam waktu yang lama menjadi penyebab terjadinya eutrofikasi dan kematian organisme. Proses reduksi nitrogen pada air limbah dapat dilakukan secara ekonomis dan efisien menggunakan bakteri nitrifikasi-denitrifikasi. Teknologi ramah lingkungan yang juga dapat digunakan adalah bioremediasi menggunakan mikroalga seperti Chlorella sp. Adanya hubungan sinergis dari bakteri dan mikroalga berpotensi meningkatkan kinerja reduksi senyawa nitrogen pada air limbah organik. Penelitian yang dilakukan pada bulan Juli hingga Agustus 2020 ini, bersifat eksperimental laboratoris menggunakan Rancangan Acak Kelompok, bertujuan untuk mengetahui laju nitrifikasi dan perubahan kadar nitrat, laju pertumbuhan Chlorella sp., serta pengaruh perbedaan perlakuan dan waktu terhadap perubahan kadar nitrat pada air limbah organik dan hubungan Chlorella sp. dengan bakteri nitrifikasi-denitrifikasi. Air limbah yang diambil berasal dari danau Rawa Pening. Hasil penelitian menunjukkan, laju nitrifikasi secara berturut-turut dari yang tertinggi hingga terendah yaitu perlakuan C 0,2 mg NH4-N/liter/jam, diikuti perlakuan B, D dan A masing-masing 0,169, 0,009 dan 0,008 mg NH4-N/liter/jam. Pola pertumbuhan Chlorella sp. mengalami peningkatan hingga hari puncak (hari ke 6) dan menurun hingga hari terakhir. Persentase perubahan kadar nitrat dari awal hingga akhir pengamatan dari yang tertinggi secara berurutan yaitu perlakuan C (1874%), B (1664%), D (200%) dan A (175%). Hasil analisis sidik ragam menunjukkan terdapat perbedaan antara perlakuan terhadap perubahan kadar nitrat (p value <0,05). Hasil Uji DMRT menunjukkan rata-rata perubahan tertinggi adalah perlakuan C, B, D dan A.

scholarly journals Pengaruh Terapi Musik Klasik terhadap Respon Fisiologis pada Pasien yang Mengalami Kecemasan Praoperatif Ortopedi

2012 ◽  
Vol 1 (4) ◽  
pp. 205-209
Author(s):  
Armansyah . ◽  
Yecy Anggreny
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Music Therapy ◽  
Classical Music ◽  
Physiological Responses ◽  
Respiratory Frequency ◽  
P Value ◽  
Relaxation Effects ◽  
The Mind ◽  
Patients Experience

Preparation of patients mental before to surgery is needed. If patients experience severe anxiety and panic operating plan will be delayed, it will allow a greater risk of infection. From various research show that music has a strong influence on health, especially in calming the mind, reduce physical tension and create a relaxed state, if the patient in a relaxed state is expected to affect physiological responses. The purpose of this study was to determine the effect of classical music therapy on physiological responses in patients who underwent orthopedic praoperatif anxiety. The study design is pre-experiment with a sample of 30 people using purposive sampling. Music therapy is given for ± 30 minutes. The analysis used were Paired Sample T Test and Wilcoxon. Obtained mean systolic blood pressure was 120.2 mmHg before therapy, after therapy 119.6 mmHg, p value = 0.227. Mean diastolic blood pressure before treatment was 74.1 mmHg, 73.2 mmHg after therapy is, p value = 0.133. Mean heart rate before treatment was 81.8 x / min, after therapy was 79 x / minute, p value = 0.005. Median respiratory frequency before treatment was 23 x / minute, after the therapy is 21 x / minute, p value = 0.001. This shows the classical music therapy has an influence on heart rate and respiratory frequency in patients who underwent orthopedic praoperatif anxiety. Researchers recommend the use of classical music therapy with a frequency of 2 to 3 times a day before patients undergo surgery in order to obtain an optimal relaxation effects.

scholarly journals Sales Territory Design and Salesforce Performance in the Detergent Manufacturing Companies: The Actual Kenyan Context

2021 ◽  
Vol 17 (35) ◽  
pp. 203
Author(s):  
Benson Muchoki Mwangi ◽  
Francis N. Kibera ◽  
Mary Kinoti ◽  
Magutu P. Obara
Keyword(s):  
Critical Role ◽  
Strong Relationship ◽  
Primary Data ◽  
P Value ◽  
Manufacturing Companies ◽  
Cross Sectional ◽  
Territory Design ◽  
Simple Regression Model ◽  
Independent Variable ◽  
Cross Sectional Design

This paper focuses on determining the influence of sales territory design on salesforce performance in the detergent manufacturing companies in Kenya. Detergents play a critical role in our everyday lives by ensuring hygiene at our personal level, in our homes, in schools, institutions, and hospitals. Detergents have gained a lot of prominence since the onset of the Corona Virus (COVID-19) disease in 2019, as it has been proved that they play an important role in curtailing the transmission of the COVID-19 disease. The Salesforce plays an important role in organizations as they are the ones who in most cases interact with customers, provide information about their organization and products, provide prices, demonstrate how products are used, train customers, provide after sales service, and resolve any issues that may arise. The study was anchored on Expectancy Theory, Resource Based Theory, Equity Theory, and Agency Theory. The study adopted positivism philosophy and used a descriptive cross sectional design. The unit of analysis was the sales people in the detergent manufacturing companies in Kenya who are members of Kenya Association of Manufacturers as per list obtained from KAM in December 2018. The study used primary data which was collected by administering semi-structured questionnaires. The data was analyzed using a combination of both descriptive and inferential statics to describe the manifestations of the variables in the data collected, simple regression model was used to test the significance of the influence of sales territory design (independent variable) on the salesforce performance (dependent variable). Fischer distribution test (F-test) was used to test the significance of the independent variable and the overall model. The p-value for the F-statistic was used to determine the robustness of the model. This was done at 95% confidence level (p<0.05). The study found a strong relationship between sales territory design and salesforce performance. This depicts that sales territory design is critical in determining salesforce performance in detergent manufacturing companies in Kenya.

scholarly journals EFFECTIVENESS OF LAVENDER AROMATHERAPY AND CLASSICAL MUSIC THERAPY IN LOWERING BLOOD PRESSURE IN PREGNANT WOMEN WITH HYPERTENSION

2017 ◽  
Vol 3 (6) ◽  
pp. 750-756
Author(s):  
Sri Maisi ◽  
Suryono Suryono ◽  
Melyana Nurul Widyawati ◽  
Ari Suwondo ◽  
Suryati Kusworowulan
Keyword(s):  
Blood Pressure ◽  
Music Therapy ◽  
Systolic Blood Pressure ◽  
Pregnant Women ◽  
Classical Music ◽  
Combination Group ◽  
Control Group ◽  
P Value ◽  
The Mean ◽  
And Control

Background: Hypertension during pregnancy remains high in Indonesia. It is a major cause of maternal death. Aromatherapy lavender and classical music therapy are considered effective in lowering blood pressure in hypertension.Objective: To examine the effect of lavender aromatherapy and classical music therapy in lowering blood pressure in pregnant women with hypertension.Methods: A quasy experimental study with pretest-posttest control group design. There were 52 pregnant women with the inclusion criteria selected as samples using simple random sampling, divided into lavender aromatherapy group, classical music group, combination of aromatherapy and music group, and control group. Sphygmomanometer was used to measure blood pressure. Mann Whitney and Post Hoc test were used for data analysis.Results: Results showed that four groups have a significant decrease in systolic blood pressure after given intervention with p-value <0.05. The mean decrease of systolic blood pressure among four groups was: lavender group (5.77 mmHg), music group (7.23 mmHg), combination group (9.54 mmHg), and control group (3.67 mmHg); and the mean decrease of diastolic blood pressure was: the lavender group (2.77 mmHg), music group (0.61 mmHg), combination group (8.23 mmHg), and control group (3.42 mmHg).Conclusion: there was a significant effect of lavender aromatherapy and classical music therapy in lowering blood pressure in pregnant women with hypertension. However, the combination of both interventions was more effective than lavender aromatherapy or music therapy alone.

scholarly journals Kepemimpinan Intrapreneurship, Budaya Sekolah dan Kinerja Inovasi Guru

2017 ◽  
Vol 5 (2) ◽  
pp. 176-193
Author(s):  
Agus Wibowo ◽  
Ari Saptono
Keyword(s):  
Elementary School ◽  
School Culture ◽  
Elementary School Teachers ◽  
Correlation Coefficient ◽  
Innovation Performance ◽  
Coefficient Of Determination ◽  
Survey Method ◽  
P Value ◽  
School Teachers ◽  
The Relationship

This article aims to determine the relationship intrapreneurial leadership, school culture and innovation performance of elementary school teachers in East Jakarta. This research uses survey method with correlation approach. The results showed that there was a significant correlation between intrapreneurial leadership and school culture on teacher innovation performance of elementary school in East Jakarta where the result of double correlation coefficient test (Ry.12) = 0,885 and F count (F Change) = 266,395, and p -value = 0,000 <0.05, and While the coefficient of determination (R square) = 0,784, which means that the intrapreneurial leadership (X1) and school culture (X2) together influence 78,4% to the performance of innovation (Y). Keywords: Intrapreneurial Leadership, School Culture, Teacher's Innovation Performance

Abstract 361: Neuregulin Transcriptional Programming of Embryonic Endothelial Progenitor Cells and Embryonic Stem Cells

2012 ◽  
Vol 111 (suppl_1) ◽  
Author(s):  
Jijun Hao ◽  
Cristi L Galindo ◽  
Radwan N Safa ◽  
Truc-Linh Tran ◽  
Douglas B Sawyer
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Heart Development ◽  
Progenitor Cell ◽  
Critical Role ◽  
Embryonic Stem ◽  
Mapk Signaling ◽  
P Value ◽  
Type I ◽  
Endothelial Progenitor

Jijun Hao, Cristi L. Galindo, Radwan N. Safa, Truc-Linh Tran, Douglas B. Sawyer Neuregulin-1 (NRG-1) plays a critical role in heart development by signaling through type I receptor tyrosine kinases in the erbB family (erbB2, erbB3 and erbB4). Mice with disrupted expression of NRG-1, ErbB2, ErbB3 or ErbB4 die in utero with failure of cardiac development. We have previously shown that NRG-1 has distinct effects on two embryonic progenitor cell populations that express ErbB2 and ErbB3 receptors. In an embryonic endothelial progenitor cell line (eEPCs) NRG-1 treatment induces phosphorylation of Akt, GSK-3β, and Erk1/2, and protects eEPCs against serum deprivation-induced apoptosis. In embryonic stem cells (ESCs) we find that NRG-1 treatment from day 0∼2 induces cardiomyocyte formation by day 8 in culture, and when ErbB3 is knocked down in the ESCs, NRG-1 fails to promote cardiomyogenesis. To understand early molecular events that might regulate these distinct effects, we analyzed global transcriptional changes induced by NRG-1 in both eEPCs and ESCs using microarrays. There were only 244 significantly differential (p value < 0.05, fold-change > 1.5) genes detected in NRG-1-treated ESCs, while NRG-1 induced differential expression of 1,547 transcripts in eEPCs. Based on functional analysis, the most significantly over-represented function (Fishers Exact Test, p value with FDR < 0.05) in ESCs was “cell morphogenesis during differentiation”. In eEPCs, genes regulated via Ras/MAPK signaling were altered, as were those downstream of the Akt-PI3K pathway and calcium signaling. For both cell lines, the most statistically significant transcription factor identified as a regulator of the genes altered in response to NRG-1 was SRF, consistent with a role for NRG-1 in heart development and regeneration. Based on the results of this study, we constructed a putative signaling pathway whereby NRG mediates cardiomyogenesis in pluripotent stem cells that correlates with phenotypic observations.

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