scholarly journals Propagation of pariparoba (Piperaceae) by different types of stem cuttings and substrates

2019 ◽  
Vol 6 (3) ◽  
pp. 75
Author(s):  
Aurea Portes Ferriani ◽  
Diones Krinski

Piper cernuum Vell., a native species known as pariparoba, presents essential oil with potential properties and significant growth in clearings, being indicated for the recovery of degraded areas. Prospecting studies for new botanical matrices need the development of specific essays of plant propagation for future domestication of the species. This work aimed to evaluate the rooting of different types of cuttings using different substrates to develop a seedling production protocol. Stem cuttings with one or two nodes were installed on three types of substrates (medium sifted sand, vermiculite of medium granulometry and commercial substrate [Tropstrato HP®]) and maintained in greenhouse for 60 days to evaluation of the percentages of survival, mortality, sprouts, rooting, average number and average length of the roots. The results showed interaction between cutting types and substrates for rooting percentage and sprouting cuttings. The largest rooting percentages were observed in apical cuttings planted in sand and vermiculite (57.0 and 55.25%, respectively). Cuttings with two nodes presented higher performance than those with one node when compared for the analyzed variables. 

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Aurea Portes Ferriani ◽  
Diones Krinski

Forests constitute a valuable natural resource, especially when including non-timber forest products (NTFPs). Bioprospecting for and sustainable exploration of native species and the development of protocols for seedling production promote conservation. Piper aduncum L. stands out in several regions of Brazil because of several biological activities, notably action as a repellent, antimicrobial and insecticide. This study aimed to evaluate the influence of defoliation and different substrates on the cutting process. For this, an experiment was conducted in February, 2016 at the Health Plant Department, Sector of Agricultural Sciences, Federal University of Paraná (UFPR). Piper aduncum stem cuttings with 10 cm and 1/3 of the leaf area and defoliated cuttings. Stem cuttings were washed in running water for 5 minutes for subsequent use in three different substrates (medium sand sifted, vermiculite and commercial substrate Tropstrato HP®). The experiment design was completely randomized in a 2×3 factorial arrangement (cutting type x substrate), and it was evaluated the rooting percentage, mortality, shoot emission, number of roots and average length of the three largest roots after 60 days. The results confirmed that the presence of leaves in P. aduncum stem cuttings promotes adventitious rooting. The different substrates did not exert a significant influence on the performance of the cuttings in this species.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 930
Author(s):  
Xu Yu ◽  
Xiwu Qi ◽  
Shumin Li ◽  
Hailing Fang ◽  
Yang Bai ◽  
...  

Light is a key environmental aspect that regulates secondary metabolic synthesis. The essential oil produced in mint (Mentha canadensis L.) leaves is used widely in the aromatics industry and in medicine. Under low-light treatment, significant reductions in peltate glandular trichome densities were observed. GC-MS analysis showed dramatically reduced essential oil and menthol contents. Light affected the peltate glandular trichomes’ development and essential oil yield production. However, the underlying mechanisms of this regulation were elusive. To identify the critical genes during light-regulated changes in oil content, following a 24 h darkness treatment and a 24 h recovery light treatment, leaves were collected for transcriptome analysis. A total of 95,579 unigenes were obtained, with an average length of 754 bp. About 56.58% of the unigenes were annotated using four public protein databases: 10,977 differentially expressed genes (DEGs) were found to be involved in the light signaling pathway and monoterpene synthesis pathway. Most of the TPs showed a similar expression pattern: downregulation after darkness treatment and upregulation after the return of light. In addition, the genes involved in the light signal transduction pathway were analyzed. A series of responsive transcription factors (TFs) were identified and could be used in metabolic engineering as an effective strategy for increasing essential oil yields.


2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Ping Jia ◽  
Ting Gao ◽  
Hua Xin

The types, morphology, distribution, structure, and development process of the glandular trichomes on the leaves ofThymus quinquecostatusCelak had been investigated in this study. Two different types of glandular trichomes were determined in detail, namely, capitate trichomes and peltate ones. Besides, there were distinct differences on morphology, distribution, structure, and development process between the two kinds of trichomes. As the peltate trichome stepping into senium stage, it caved in the epidermis integrally, which was different from the capitate one. The secretion of the capitate trichome contained essential oil, polyphenols, and flavonoids, while, in addition to these three components, the secretion of the peltate one also contained acid polysaccharides. A distinctive difference was also seen in the secretory pathway of the secretion between the two types of trichomes. The secretion of capitate one was extruded through the cuticle of the head cell, but the secretion of the peltate one kept accumulating in the subcuticular space of the head cells until it was released by cuticle rupture.


2020 ◽  
Vol 9 (9) ◽  
pp. e995998389
Author(s):  
Matheus Novaes Valinho ◽  
Jhenifer Terezinha Aparecida Mattos Cescon ◽  
Ana Paula Roem Simoni ◽  
Lucília de Lourdes Pellozo Zambrotti

Since the beginning of operations in 2014, Port of Açu has shown significant growth in relation to cargo handling, with all demand dependent on road transport. However, there is a proposal for the implementation of the EF-118 railway that would interconnect Rio de Janeiro and Vitória, serving the region of Port. This work seeks to economically evaluate the advantages of the implementation of the railway modal compared to the roadway already used in the region. Due to the intense flow of cargo in the Industrial-Port Complex, the possibility of building a new highway, the RJ-244, is being studied, extending from the industrial district to BR-101. For this purpose, surveys were conducted to determine the average daily volume of vehicles circulating in the region, in addition to the different types of cargo transported and their relationship with Port. Based on the information collected in the Traffic Studies Report issued, it was possible to relate data and achieve at the value of the average annual daily volume for each category of cargo handled in the Port. Bearing in mind that in 2019, Port of Açu handled approximately 751 thousand tons of cargo and analyzing the estimated costs by ABIFER for road and rail transport, the approximate cost values for each one per ton of cargo per kilometer were reached. Comparing values, it is possible to notice that the cost of transporting cargo by rail corresponds to about 16.6% of the amount spent on transporting of the same weight of cargo by road, which means a considerable advantage for its implementation.


2011 ◽  
Vol 83 (4) ◽  
pp. 1279-1286 ◽  
Author(s):  
Rita de Cássia S.A. Canteiro ◽  
Edélti F. Albertoni

Chironomidae larvae are important macroinvertebrates in limnic environments, but little knowledge exists about their biometrics development characteristics. This study aims to describe the immature Chironomus calligraphus Goeldi, 1905 under laboratory conditions by the accomplishment of thirteen egg masses from eggs eclosion to adults emergency, at controlled room temperature (25ºC) and photoperiod (12-12h). Larvae were feed ad libitum with "Alcon Basic - MEP 200 Complex" fish food and commercial dehydrated Spirulina. The postures had a mean length of 9 ± 1 mm (n = 13) and 348 ± 66 eggs. The brownish colored eggs with elliptical shape had length of 160.3 ± 17.7 µm (n = 130), being arranged as an organized string in a pseudo spiral form. The time duration from the first to the four instars were three, four, four and eight days, and the average length of a cephalic capsule to each one of the instars (66.3 ± 12.3 µm, 102.9 ± 22.1 µm, 159 ± 24.6 µm, 249.2 ± 29.7 µm, n = 456) were significantly different (ANOVA, p < 0.001). The Dyar’s Rule showed a constant growth rate, r = 1.5. Our results demonstrated that C. calligraphus is a species with short life cycle, low mortality rate, food adaptability, fast larval growth and easily maintained at laboratory, factors that allowed the use of this native species as a tool for ecotoxicological tests.


2018 ◽  
Vol 47 (2) ◽  
pp. 282-287
Author(s):  
Anastasia AKOUMIANAKI-IOANNIDOU ◽  
Ekaterini GERASIMIDOU ◽  
Alexandra SALTA ◽  
Ioannis ROUSSIS ◽  
Dimitrios BILALIS

Hypericum empetrifolium Willd. subsp. empetrifolium is an evergreen small shrub with small elongated decorative leaves and small yellow flowers in inflorescences, characterized for several pharmaceutical properties. In the present study, a first approach on the sexual and asexual propagation of this species was performed. Seeds, subjected to different types of pre-treatments [soaked in tap water; 50, 100 or 150 mg L-1 GA3 for 30 min and no treatment (control)], cultured for germination in petri dishes at 5, 10, 15, 20, 25, and 30 °C. Seed germination was only affected by temperature and the best result was obtained at 15 °C (71.2%). A significant interaction was found between pre-treatments and incubation temperature with the highest germination percentage (82%) occurred when the seeds soaked in 100 mg L-1 GA3 solution for 30 min and incubated at 20 °C constant temperature. Moreover, the germination speed was fastest from 20 to 25 °C (T50= 9.84 and 9.56 days for 20 and 25 °C, respectively). For asexual propagation, apical stem cuttings were taken at four different periods (4 seasons) and treated with IBA at concentrations of 0, 1000, 2000 and 3000 mg L-1. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Winter was the most appropriate season for cuttings collection (100% rooting percentage) and dipping in 1000 or 2000 mg L-1 IBA (72% and 73%, respectively) was the best rooting hormone treatment.


2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Claudia Giuliani ◽  
Roberto Maria Pellegrino ◽  
Bruno Tirillini ◽  
Laura Maleci Bini

The essential oils from both leaves (L) and flowers (F) of Stachys germanica subsp. salviifolia (Ten.) Gams (Labiatae/Lamiaceae) from Italy were analyzed by GC-MS for the first time. The distribution, morphology and histochemistry of the different types of glandular trichomes present on the epidermal surface were also determined. Twenty-nine constituents, representing 89.4% of the total leaf oil, and forty-one compounds, forming 97.8% of the total flower oil, were identified. Germacrene D (39.4%), phytol (10.2%), β-bourbonene (3.5%) and β-ylangene (3.3%) were recognized as the main constituents of the leaf essential oil, while limonene (24.1%), β-pinene (18.7%), germacrene D (12.8%) and ( E)-nerolidol (6.6%) were the main compounds of the flower essential oil.


Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 241 ◽  
Author(s):  
Jana Jurová ◽  
Martina Matoušková ◽  
Anna Wajs-Bonikowska ◽  
Danuta Kalemba ◽  
Marek Renčo ◽  
...  

Impatiens parviflora is non-native invasive plant species occupying large areas all over the Europe and threatens native communities by altering their species composition and reducing native biodiversity. The factor responsible for its spreading could be explained by releasing biochemical to the environment. On the other hands, high demand on secondary metabolites as potential source of new ecofriendly biocides could be beneficial. The analysis of I. parviflora essential oil (EO) led us to identify more than 60 volatiles. The main compound was hexahydrofarnesyl acetone, other dominant components were phytol, carvacrol, germacra-4(15),5,10(14)-trien-1-α-ol, and pentacosane. The potential phytotoxic effect of I. parviflora EO collected in two vegetation periods (summer and autumn) was evaluated on seed germination and root elongation of three dicot species (Raphanus sativus, Lepidum sativum, and Lactuca sativa) and on one monocot species (Triticum aestivum). The seed germination of only one dicot species, L. sativa, was affected by both EOs. In contrast, seed germination of monocot species T. aestivum was influenced only by the highest doses of EOs isolated from I. parviflora in autumn. The root elongation of tested plant species was less influenced by I. parviflora EOs. L. sativum showed sensitivity to one dose of EOs hydrodistilled in summer, while the monocot species was influenced by both EOs samples in highest doses. Our findings revealed that I. parviflora contained phenolics that were phytotoxic to the germination of some plant species, mainly at higher EOs doses, while root elongation of tested plants was not suppressed by essential oils.


2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Romana Chovanová ◽  
Mária Mikulášová ◽  
Štefánia Vaverková

The essential oil (EO) fromSalvia sclareawas shown to increase the susceptibility of methicillin resistantStaphylococcus epidermidis(MRSE) isolates to oxacillin. The purpose of this study was to investigate the effect of EO fromS. sclareaon expression ofmecA gene of MRSE carrying different types of staphylococcal chromosomal cassette (SCCmec) and to evaluate potential synergistic effect of EO with oxacillin. Using real-time PCR we found that EO alone inhibited the expression of the resistant genesmecA,mecR1, andmecI andblaZ,blaR1, andblaI. The use of the combination of EO with oxacillin resulted in significantly inhibited expression ofmecA gene in all tested strains with different types of SCCmec. Using time-kill assay and checkerboard assay we confirmed synergistic effect of EO fromS. sclareaand oxacillin in MRSE.


1990 ◽  
Vol 30 (5) ◽  
pp. 681 ◽  
Author(s):  
E Diatloff

Three native species of Leptospermum (L. petersonii, L. Flavescens, L. luehmannii) were grown in an amended podsolic soil under 4 nitrogen (N) levels, 0, 20, 40 and 60 kg Nha, supplied as NH4N03. Plants were harvested after 16 weeks growth, leaves were separated from stems, and essential oil was distilled from leaves by steam distillation. Extracted oils were analysed for their components by gas chromatography. Oil density from L. petersonii was also determined. Growth and oil yield of the 3 Leptospermum species showed no response to N fertilisation. However, the oil composition in L. petersonii and L. flavescens leaves was affected. The greatest response was in the geranial and citronella1 contents in L. petersonii, which peaked in the 40 kg N/ha treatment. This is the first known report of the manipulation of essential oil composition of Australian native plants by N fertilisation. The low optimal level of N fertiliser required for maximum production of valuable oil components indicates that Leptospermum spp. are suitable for infertile soils. The low �-pinene yield of L. luehmannii makes it unsuitable for commercial exploitation.


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