Microencapsulation of Carvacrol Using Pectin/Aloe-gel as a Novel Wound Dressing Films

Recently, Pectin (PEC) and Aloe-Gel (AG) have received great attention for their use in the encapsulation of hydrophobic bioactive compounds such as Carvacrol (CAR). The aim of this study is to assess the physical, chemical and biological properties of a novel PEC/AG film and evaluate its capability to entrap CAR into microencapsulates. For this purpose, the casting method was used to prepare the PEC/AG membranes (70:30 % w/w). The CAR-loaded PEC/AG film was prepared adding different proportions of CAR (0.25%, 0.50% and 1.00% v/v) to the mixture of PEC/AG, previously emulsified with tween 80 (1.0%). The optical properties, Water Vapor Permeability (WVP), ATR-FTIR spectroscopy, microstructure, antibacterial activity and size of microcapsules were evaluated. The PEC/AG membranes loaded with CAR showed yellowish appearance and they were transparent to the UV electromagnetic radiation (190, 200 and 280 nm). The film prepared with the lowest amount of CAR (PC/AG-CAR-0.25%) showed the highest values of WVP (66.2%) and, according to SEM micrograph, the largest microcapsules (≈1005± 39 µm3). The FTIR analysis showed the characteristic absorption peaks located at 1015 cm-1 to 1030 cm-1 and a small shoulder to 990 cm-1 of benzene ring 1:2:4 substituted that suggested the presence of CAR-loaded in the PC/AG film. On the other hand, E. coli O157:H7 showed the highest sensitivity to the PEC/AG-CAR-1.00% film, while S. aureus was not sensitive.

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Fabrication and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) modified with nano-montmorillonite biocomposite

e-Polymers ◽

10.1515/epoly-2021-0006 ◽

2020 ◽

Vol 21 (1) ◽

pp. 038-046

Author(s):

Xu Yan ◽

Wanru Zhou ◽

Xiaojun Ma ◽

Binqing Sun

Keyword(s):

Thermal Stability ◽

Oxygen Permeability ◽

Water Vapor Permeability ◽

Barrier Properties ◽

Nucleating Agent ◽

Vapor Permeability ◽

Casting Method ◽

Increasing Trend ◽

Solution Casting Method

Abstract In this study, a poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) modified with nano-montmorillonite biocomposite (MMT/PHBH) was fabricated by solution-casting method. The results showed that the addition of MMT increased the crystallinity and the number of spherulites, which indicated that MMT was an effective nucleating agent for PHBH. The maximum decomposition peak of the biocomposites moved to a high temperature and residue presented an increasing trend. The biocomposites showed the best thermal stability at 1 wt% MMT. Compared with PHBH, 182.5% and 111.2% improvement in elastic modulus and tensile strength were obtained, respectively. Moreover, the oxygen permeability coefficient and the water vapor permeability of MMT/PHBH biocomposites decreased by 43.9% and 6.9%, respectively. It was also found that the simultaneous enhancements on the crystallizing, thermal stability, mechanical, and barrier properties of biocomposites were mainly caused by the formation of intercalated structure between PHBH and MMT.

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Application of Supercritical Solvent Impregnation for Production of Zeolite Modified Starch-Chitosan Polymers with Antibacterial Properties

Molecules ◽

10.3390/molecules25204717 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4717 ◽

Cited By ~ 1

Author(s):

Jelena Pajnik ◽

Ivana Lukić ◽

Jelena Dikić ◽

Jelena Asanin ◽

Milan Gordic ◽

...

Keyword(s):

Release Kinetics ◽

Antibacterial Properties ◽

Water Vapor Permeability ◽

Weibull Model ◽

Vapor Permeability ◽

E Coli ◽

Supercritical Solvent ◽

Composite Polymers ◽

Before And After ◽

Impregnation Time

In the present study, supercritical solvent impregnation (SSI) has been applied to incorporate thymol into bio-composite polymers as a potential active packaging material. Thymol, a natural component with a proven antimicrobial activity, was successfully impregnated into starch-chitosan (SC) and starch-chitosan-zeolite (SCZ) films using supercritical carbon dioxide (scCO2) as a solvent. Experiments were performed at 35 °C, pressures of 15.5 and 30 MPa, and an impregnation time in the range of 4–24 h. The highest impregnation yields of SC films with starch to chitosan mass ratios of 1:1 and 1:2 were 10.80% and 6.48%, respectively. The addition of natural zeolite (15–60%) significantly increased the loading capacity of films enabling thymol incorporation in a quantity of 16.7–27.3%. FTIR and SEM analyses were applied for the characterization of the films. Mechanical properties and water vapor permeability of films before and after the impregnation were tested as well. Thymol release kinetics in deionized water was followed and modeled by the Korsmeyer-Peppas and Weibull model. SCZ films with thymol loading of approximately 24% exhibited strong antibacterial activity against E. coli and methicillin-resistant Staphylococcus (S.) aureus (MRSA).

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Effect of Surfactant on the Formation of Chitosan/ε-Polycaprolactone Blend Films for Food Packaging Applications

10.21203/rs.3.rs-804708/v1 ◽

2021 ◽

Author(s):

Ana Catarina Damasceno Gomes ◽

Karoline Ferreira Silva ◽

Anderson Junior Freitas ◽

Kelvi Wilson Evaristo Miranda ◽

Taline Amorim Santos ◽

...

Keyword(s):

Food Packaging ◽

Water Vapor Permeability ◽

Tween 80 ◽

Continuous Phase ◽

Vapor Permeability ◽

Mechanical And Thermal Properties ◽

Amino Groups ◽

Multilayer Systems ◽

Blend Films ◽

High Interaction

Abstract The objective of this study was to develop and characterize chitosan-ε-polycaprolactone (CHI/PCL) polymer blends with Tween 80 as a compatibilizer for application in packaging. The blends were produced by casting, with up to 10% (w/w) PCL in the CHI matrix. These blends were characterized in terms of their microstructure, chemical interactions, mechanical and thermal properties, solubility and water vapor permeability (WVP). The micrographs showed microsphere-like structures from the PCL in the continuous phase of CHI. Fourier transform infrared spectroscopy (FTIR) indicated a high interaction between CHI amino groups and PCL carbonyls, resulting in blends with greater ductility than the pure CHI film, thus providing greater flexibility. In the crystallinity analyses, the presence of PCL favored an increase in crystalline regions, limiting the passage of light. Given the results, blends are an alternative for application in multilayer systems and can be considered for use as a biodegradable fraction in food packaging.

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Antibacterial and biological properties of coconut oil loaded poly(ε-caprolactone)/gelatin electrospun membranes

Journal of Industrial Textiles ◽

10.1177/1528083721991595 ◽

2021 ◽

pp. 152808372199159 ◽

Cited By ~ 1

Author(s):

Parian S Mohamadi ◽

Ahmad Hivechi ◽

Hajir Bahrami ◽

Nahid hemmatinegad ◽

Peiman B Milan

Keyword(s):

Antibacterial Activity ◽

Water Vapor ◽

Coconut Oil ◽

Water Vapor Permeability ◽

Biological Properties ◽

Morphological Properties ◽

Vapor Permeability ◽

Crosslinking Reaction ◽

Water Contact ◽

Bio Oil

Coconut oil (CO) is a naturally derived bio-oil which exhibits specific characteristics such as biocompatibility and antibacterial activity. In this work, the biological properties of poly(caprolactone)/gelatin (PCL/Gel) nanofibers are improved using CO encapsulation. This bio-oil was added to the PCL/Gel polymer solution with different concentrations (5–40%). Nanofibers were crosslinked using glutaraldehyde vapor. Different types of characterization techniques such as SEM, FTIR, DSC, tensile measurements, water contact angle, and water vapor permeability were used to study the chemical, physical, thermal, and morphological properties of resultant nanofibers. Results showed an average diameter of 300–370 nm for as-spun nanofibers, which increased to 360–470 nm after the crosslinking reaction. The presence of CO was confirmed using FTIR and DSC experiments. Moreover, results indicated that the presence of CO increases the hydrophilicity and water vapor permeability of nanofibers, which are desirable for their final application. Biological tests, such as antibacterial activity, cell viability, and cell morphology tests were performed to evaluate the possible application of the produced nanofibers for wound healing applications. Results indicated that the crosslinked PCL/Gel nanofibers containing 20% CO exhibited the highest cell compatibility and antibacterial activity against gram-positive (S. aureus) and gram-negative ( E. coli) bacteria.

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IMAGENS DIGITAIS NO ESTUDO DO ENVELHECIMENTO DO QUEIJO MINAS FRESCAL: UM ESTUDO COMPARATIVO SOBRE DIFERENTES EMBALAGENS

e-xacta ◽

10.18674/exacta.v9i1.1622 ◽

2016 ◽

Vol 9 (1) ◽

pp. 23

Author(s):

Amanda Ribeiro de Jesus ◽

Ana Clara Vidotti ◽

Angela Maria Fernandes ◽

Demetrius Dias Caldas Lopes ◽

Fernanda Maria Vitor Silva ◽

...

Keyword(s):

Outer Part ◽

Microbiological Quality ◽

Water Vapor Permeability ◽

Principal Component ◽

Vapor Permeability ◽

Color Changes ◽

Physical Chemical ◽

Fresh Cheese ◽

Belo Horizonte

O objetivo do presente trabalho foi avaliar a influência de sacos plásticos de Polietileno de Baixa Densidade (PEBD) e embalagens a vácuo sobre a cor, a qualidade físico-química e microbiológica do queijo Minas frescal. Foram avaliados 10 queijos provenientes de 5 produtores artesanais da cidade de Belo Horizonte, adquiridos 2 dias após a fabricação. Os queijos foram divididos em dois grupos, cada qual com 15 unidades (três pedaços de cinco queijos), e acondicionados em geladeira a temperatura de 6 ºC até o momento dos testes. As análises de pH, teor de umidade, teor de gordura, microbiológicas e o estudo da cor foram feitos nos dias 0, 10 e 20, tanto da parte interna, quanto da parte externa do produto. O uso de ferramentas quimiométricas, como o Image Principal Componente Analysis (PCA de imagens), permitiu evidenciar alterações de cor das amostras no período de observação e correlacioná-las com os dois tipos de embalagens utilizados e com os valores obtidos nas análises físico-químicas. Os resultados apontaram que as características das embalagens relacionadas à permeabilidade a gases e vapor d’água propiciaram alterações físico-químicas e microbiológicas nas amostras, resultando em diferentes tonalidades de amarelo perfeitamente evidenciadas pelo PCA, sendo que a embalagem a vácuo se demonstrou mais eficaz na conservação das propriedades avaliadas. A contaminação microbiológica das amostras ressaltou a importância das boas práticas de fabricação (BPF) na qualidade do queijo Minas frescal.AbstractThe purpose of this paper was to evaluate the influence of Low Density Polyethylene plastic bags (LDPE) and vacuum packaging on the color, physicochemical and microbiological quality of Minas fresh cheese. 10 cheeses from 5 artisan producers from the city of Belo Horizonte were acquired two days after fabrication. The cheeses were divided into two groups, each one with 15 units (three pieces of five chesses), and stored in a refrigerator at 6 ° C until the day of analysis. The analysis of pH, humidity content, fat content, microbiological and the study of color were made on days 0, 10 and 20, both in the inner and the outer part of the product. The use of chemometric tools, such as Principal Component Image Analysis (Image PCA), was able to detect the color changes of the samples during the observation period and correlate them with the two types of packaging and the variations on the physical chemical parameters. The results showed that the characteristics of the packages related to gases and water vapor permeability have provided physical, chemical and microbiological changes in the samples, resulting in different shades of yellow, perfectly highlighted by the PCA. Microbiological contamination of the samples stressed the importance of good manufacturing practices (GMP) on the quality of Minas fresh cheese.

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Physico-Mechanical and Antibacterial Properties of PLA/TiO2 Composite Materials Synthesized via Electrospinning and Solution Casting Processes

Coatings ◽

10.3390/coatings9080525 ◽

2019 ◽

Vol 9 (8) ◽

pp. 525 ◽

Cited By ~ 14

Author(s):

Shiyi Feng ◽

Feng Zhang ◽

Saeed Ahmed ◽

Yaowen Liu

Keyword(s):

Water Vapor ◽

Food Packaging ◽

Water Solubility ◽

Antibacterial Properties ◽

Water Vapor Permeability ◽

Vapor Permeability ◽

Solution Casting ◽

Water Contact ◽

E Coli ◽

Composites Materials

In this study, PLA/TiO2 composites materials were prepared via electrospinning and solution casting processes. By testing the mechanical properties, water contact angle, water vapor permeability, and solubility of the composite nanofibers and films, the comprehensive performances of the two types of nanocomposites were analyzed. The results show that maximum tensile strengths of 2.71 ± 0.11 MPa and 14.49 ± 0.13 MPa were achieved for the nanofibers and films at a TiO2 content of 0.75 wt.%. Moreover, the addition of TiO2 significantly cut down the water vapor transmittance rate of the nanofibers and films while significantly improving the water solubility. Further, the antibacterial activity increased under UV-A irradiation for a TiO2 nanoparticle content of 0.75 wt.%, and the nanofiber and films exhibited inhibition zones of 4.86 ± 0.50 and 3.69 ± 0.40 mm for E. coli, and 5.98 ± 0.77 and 4.63 ± 0.45 mm for S. aureus, respectively. Overall, the performance of the nanofiber was better than that of the film. Nevertheless, both the nanocomposite membranes satisfied the requirements of food packaging materials.

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Physical-Chemical Properties of Edible Film Made from Soybean Residue and Citric Acid

Journal of Chemistry ◽

10.1155/2018/4026831 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Wenjun Ma ◽

Sami Rokayya ◽

Liang Xu ◽

Xiaonan Sui ◽

Lianzhou Jiang ◽

...

Keyword(s):

Citric Acid ◽

Food Packaging ◽

Chemical Properties ◽

Water Vapor Permeability ◽

Edible Films ◽

Edible Film ◽

Sodium Hypophosphite ◽

Vapor Permeability ◽

Physical Chemical ◽

Aqueous Extraction Processing

The effect of citric acid on the properties of soybean enzyme-assisted aqueous extraction processing (EAEP) residue edible film was studied. The washed soybean EAEP residue was produced by the process of EAEP. It was determined that the washed soybean EAEP residue is rich in fibers (76.10 ± 1.03%) and has lower oil and protein contents (7.74 ± 0.11% and 3.50 ± 0.20%, resp.). Edible films intended for food packaging have been produced from the washed EAEP residue combined with glycerol, different concentrations of citric acid (0%, 10%, 15%, 20%, 25%, and 30%), and sodium hypophosphite. The spectra have evidenced that the cross-linking reaction of citric acid and fibers has taken place in the residue. 30-CA films showed the highest tensile strength (17.52 MPa) and the lowest water vapor permeability (7.21 g·cm−1·s−1·Pa−1). Also, it indicated that citric acid can cross-link with the hydroxyls of polysaccharide and improve the compatibilization between the polymeric molecules to improve the intermolecular interaction between polysaccharide molecules, so that the water uptake is reduced. The smooth surface and better translucency of the films suggest that the EAEP residue films treated with citric acid are suitable for application in food packaging.

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SIFAT TRANSPARANSI DAN PERMEABILITAS FILM BIONANOKOMPOSIT POLYLACTIC ACID DAN POLYCAPROLACTONE DENGAN PENAMBAHAN NANOCRYSTALLINE CELLULOSE SEBAGAI PENGISI [Transparency and permeability properties of Bionanocomposite Film of Polylactic Acid and Polycaprolactone, and Nanocrystalline Cellulose as a Filler]

Jurnal Teknologi & Industri Hasil Pertanian ◽

10.23960/jtihp.v25i2.81-89 ◽

2020 ◽

Vol 25 (2) ◽

pp. 81

Author(s):

Eti Indarti ◽

Arisa Sri Marlita ◽

Zaidiyah Zaidiyah

Keyword(s):

Water Vapor ◽

Polylactic Acid ◽

Water Vapor Permeability ◽

Nanocrystalline Cellulose ◽

Vapor Permeability ◽

Solvent Casting ◽

Casting Method ◽

Empty Fruit Bunches ◽

Bionanocomposite Films

Production of Polylactic acid (PLA)/Polycaprolactone (PCL) bionanocomposite films with various ratios was done by adding nanocrystalline celullose (NCC) from oil palm empty fruit bunches (OPEFB) as a filler. The aim of the research was to find out the effect of PLA/PCL ratio on film thickness, transparency of bionanocomposite films and water vapor permeability or WVP of the film bionanocomposite with addition of the 3% NCC. The PLA/PCL ratio are 1.0/0.0; 0.8/0.2; 0.6/0.4; 0.5/0.5; 0.4/0.6; 0.2/0.8; and 0.0/1.0, prepared with solvent casting method. Characterization of PLA/PCL bionanocomposites film performed was thickness, transparency test and water vapor permeability (WVP) test. The thickness of bionanocomposites film produced were around are about 0.036-0.053 mm, results show that the lower PLA/PCL ratio the thicker film obtained. The highest value of film transparency was obtained at a ratio of 1.0 / 0.0 (81.4% at a wavelength of 550 nm), the smaller the PLA / PCL ratio, the lower the value of transparency. The WVP value of PLA/PCL bionanocomposite films gives a lower value than the WVP value of pure PLA film and pure PCL film. The best WVP was obtained at a PLA/PCL ratio of 0.8/0.2 which was 1.49x10-16kg.m/(m2.s.Pa).

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Effects of Candelilla and Carnauba Wax Incorporation on the Functional Properties of Edible Sodium Caseinate Films

International Journal of Molecular Sciences ◽

10.3390/ijms21249349 ◽

2020 ◽

Vol 21 (24) ◽

pp. 9349

Author(s):

Sabina Galus ◽

Margaux Gaouditz ◽

Hanna Kowalska ◽

Frédéric Debeaufort

Keyword(s):

Water Vapor ◽

Functional Properties ◽

Water Vapor Permeability ◽

Tween 80 ◽

Sodium Caseinate ◽

Vapor Permeability ◽

Mechanical Resistance ◽

Carnauba Wax ◽

Color Differences ◽

Lipid Particles

The aim of this study was to evaluate the effects of candelilla (CAN) or carnauba wax (CAR) incorporation on functional properties of edible sodium caseinate (CAS) films. Glycerol and Tween-80 were used as the plasticizer and the emulsifier, respectively. The results showed that the incorporation of waxes increased film opacity, total color differences (∆E), and mechanical resistance and reduced film lightness, water vapor permeability (WVP), and elongation at break. Scanning electron microscopy showed heterogeneous structure of emulsion films with regular distribution of lipid particles. A different internal arrangement was observed as a function of the film composition with both layered and incorporated film structure. Films containing candelilla wax exhibited more regular lipid reorganization, which resulted in better water vapor barrier efficacy and mechanical resistance in comparison to control films. The presence of Tween-80 resulted in better dispersion of lipid particles in film-forming solutions and lower water solubility, lightness, film opacity, and water vapor permeability, whereas the total color differences (∆E) were significantly larger and the improvement in mechanical properties was also achieved.

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Antimicrobial Activity of Lactoferrin against Foodborne Pathogenic Bacteria Incorporated into Edible Chitosan Film

Journal of Food Protection ◽

10.4315/0362-028x-71.2.319 ◽

2008 ◽

Vol 71 (2) ◽

pp. 319-324 ◽

Cited By ~ 26

Author(s):

CYNTHIA A. BROWN ◽

BAOWU WANG ◽

JUN-HYUN OH

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Chitosan Film ◽

Water Vapor Permeability ◽

Barrier Properties ◽

Antimicrobial Activities ◽

Vapor Permeability ◽

E Coli ◽

Combination Effects ◽

Chitosan Films

The objectives of this research were to develop and characterize edible chitosan film containing lactoferrin as a natural antimicrobial agent, and to investigate the combination effects of lactoferrin with lysozyme in chitosan film against the growth of Escherichia coli O157:H7 and Listeria monocytogenes. Chitosan films containing lactoferrin, lysozyme, or nisin were fabricated, and the antimicrobial concentrations were 0.5, 1, or 2 mg in a circular disc of chitosan film. Three concentrations of lactoferrin or EDTA (0.28, 0.56, or 1.12 mg per disc) were also incorporated into the chitosan film containing lysozyme to investigate the combination effects of lactoferrin. The water barrier properties of the chitosan films containing lactoferrin were characterized. The antimicrobial activities against E. coli O157:H7 and L. monocytogenes were determined using the agar diffusion assay and cell count assay. The chitosan films containing lactoferrin less than 1 mg per disc did not alter the water vapor permeability of the chitosan film. Although the film containing lysozyme exhibited significant antimicrobial activity, the incorporation of lactoferrin alone into chitosan film did not exhibit significant antimicrobial activity against both E. coli O157:H7 and L. monocytogenes. However, the combination of lactoferrin with lysozyme-containing chitosan film significantly decreased the growth of E. coli O157:H7, exhibiting a comparable effect to that of the combination of EDTA with lysozyme (P < 0.05). Furthermore, the combination of lactoferrin with lysozyme in chitosan film exhibited greater reduction in the growth of L. monocytogenes than did the combination EDTA with lysozyme, resulting in an approximate 3-log reduction.

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