Evaluation of Cytotoxic Effect, Anti-Cholinesterase, Hemolytic and Antibacterial activities of the Species Scabiosa stellata L.

2020 ◽  
Vol 16 (1) ◽  
pp. 72-79 ◽  
Author(s):  
Chaima Mouffouk ◽  
Leila Hambaba ◽  
Hamada Haba ◽  
Soumia Mouffouk ◽  
Chawki Bensouici
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Cytotoxic Effect ◽  
Colorimetric Method ◽  
Antibacterial Activities ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Butanolic Extract ◽  
Hemolytic Assay ◽  
Crude Extracts

Objective: In this study, cytotoxic effect, anticholinesterase, hemolytic and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the plant Scabiosa stellata L. were evaluated. Methods: The cytotoxicity of extracts was tested by Brine shrimp lethality method; the acetylcholinesterase inhibitory activity was performed using Ellman's colorimetric method and the hemolytic activity was assessed by spectrophotometric method towards human erythrocytes. Furthermore, the antibacterial activity was estimated by agar disk diffusion assay against ten bacterial strains. Results: The phytochemical screening of the extracts revealed the presence of several types of secondary metabolites. A significant cytotoxic effect was observed for the n-butanolic extract with 57.2 ± 0.2 % of mortality at 80 μg/mL, the ethyl acetate extract had a moderate anticholinesterase activity at 200 μg/mL. The hemolytic assay exhibited that n-butanolic and ethyl acetate extracts induce hemolysis in dose-dependent manner with values of EC50 at 37.3 ± 0.5 and 106.6 ± 0.3 μg/mL, respectively. All the crude extracts showed antibacterial activity against most tested strains, with zones of inhibition ranging from 9 to 20 mm. Conclusion: The results indicate that the extracts obtained from S. stellata can be an important source of therapeutic agents against pathological damage due to free radicals inducing neurodegenerative and infectious diseases, while n-butanolic extract could be used as a good source of alternative natural antiproliferative compounds.

Antibacterial activity of some chemical constituents from Trichilia prieuriana (Meliaceae)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Julio Issah Mawouma Pagna ◽  
Ines Michèle Kanko Mbekou ◽  
Armelle Tontsa Tsamo ◽  
Pierre Mkounga ◽  
Marcel Frese ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Shikimic Acid ◽  
Chemical Constituents ◽  
Chemical Study ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Crude Extracts

Abstract The chemical study of hydroethanolic extracts from different parts of Trichilia prieuriana (Meliaceae) led to the isolation and identification of 22 compounds: 2β,3β,4β-trihydroxypregnan-16-one (1), prieurianin (2), flindissone (3), deoxyflindissone (4), picraquassin E (5), ursolic acid (6), 3β-acetoxy-11α-hydroxyurs-12-en (7), 3β-acetoxy-urs-12-en-11-one (8), 3β-acetoxy-β-amyrin (9), friedelin-3-ol (10), 3-oxo, friedelin (11), 3-oxo, fridelin-28-ol (12), oleanolic acid (13), hederagenin (14), mixture of stigmasterol (15), β-sitosterol (16), β-sitosterol-3-O-β-glucopyranoside (17) and stigmasterol-3-O-β-glucopyranoside (18), erythrodiol (19), scopoletin (20), 4-hydroxy-3,5-dimethoxybenzoic acid (21) and shikimic acid (22). The absolute configurations and crystal structures of compounds 1 and 2 are reported herein for the first time. Crude extracts, fractions and isolated compounds were evaluated for their antibacterial activities against nine bacterial strains. Crude extracts from the root wood of T. prieuriana exhibited good antibacterial potency with minimal inhibitory concentration (MIC) values ranging from 31.25 to 500 µg mL−1 on the test bacteria. The ethyl acetate fraction from root wood and n-hexane-ethyl acetate (3:1) fraction from leaves showed a moderate antibacterial activity with MIC value of 250 μg mL−1 on all test bacteria. Isolated compounds exhibited significant antibacterial activity with MIC values ranging from 4.09 to 71.8 µm. Compounds 3, 6 and 7 were the most active with a broad spectrum of activities.

scholarly journals Phytochemical Analysis and Antibacterial Activities of Tamarix dioica Extracts

2021 ◽  
Vol 22 (1) ◽  
pp. 28-34
Author(s):  
Saddam Hussain Bughio
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Activities ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
E Coli ◽  
Standard Procedures ◽  
The Impact ◽  
And Staphylococcus Aureus ◽  
Ethyl Acetate Extracts

The objective of this study was to investigate the impact of phytochemical analysis and the antibacterial activity of extracts of stem, flowers and leaves of Tamarix dioica Roxb. ex Roth. Four solvents namely, diethyl ether, ethyl acetate, methanol and acetone were selected to obtain extracts from different parts of the plant. The organic solvent extracts were investigated for phytochemical analysis and antibacterial activity against two bacterial strains, namely Escherichia coli and Staphylococcus aureus. The result of phytochemicals revealed the presence of various constituents, such as phlobatannins, tannins, saponins, alkaloids, phenols, proteins, terpenoids, flavonoids and steroids by using standard procedures. Most of these components were present in methanol and ethyl acetate extract. Therefore, four out of two extracts, such as methanol and ethyl acetate extracts from stems, flowers, and leaves, were used to test their evidence of antibacterial activity. From this, it was observed that the methanol extracts of stem, flowers and leaves of T. dioica were highly effective together with E. coli and S. aureus with a minimum inhibitory concentration (MIC) value of 500 μg/mL. Considering that the ethyl acetate (EA) extracts from the stem, flowers and leaves of T. dioica were examined to be ineffective against E. coli and S. aureus and MIC values were not observed in two strains of bacteria.

scholarly journals Antisickling and Antibacterial Activities of Anthocleista schweinfurthii Gilg. (Gentianaceae) from Non-human Primates Pharmacopoeia in Democratic Republic of the Congo

2019 ◽  
Vol 1 (3) ◽  
pp. 14-20
Author(s):  
Samy Ngunde-te-Ngunde ◽  
Emmanuel Moke Lengbiye ◽  
Tshidibi Dipa Joseph ◽  
Kengo Velantia Franck ◽  
Ruphin D. Djolu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Stem Bark ◽  
Antibacterial Activities ◽  
Scientific Basis ◽  
Root Bark ◽  
Bacterial Strains ◽  
Republic Of The Congo ◽  
First Time

The aim of the present study was to evaluate the Chemical composition and bioactivity of Anthocleista schweinfurthii Gilg (Gentianaceae) fruits, leaves, root bark and stem bark extracts against Sickle cell disease (SCD) and associated bacteria. The antisickling and antibacterial activities were carried out using Emmel and micro-dilution methods respectively. The results revealed that the fruits, leaves, root bark and stem bark extracts  of A. schweinfurthii contains various secondary metabolites such as the alkaloids, saponins, total polyphenols, flavonoids, tannins, anthocyanis, leuco-anthocyanins and quinones.  The ethyl acetate and methanol extracts displayed antisickling activity. While, the antibacterial activity of different plant extracts tested was weak toward tested bacterial strains (CMI ≥125μg/mL). The antibacterial activity can be improved by bio-guided fractionation of the n-hexane, ethyl acetate or methanol soluble fraction. This study provides for the first time a scientific basis for the in vitro antisickling activity of A. schweinfurthii.

Antioxidant and Antibacterial Activities of Artemisia herba-alba Asso Essential Oil from Middle Atlas, Morocco

2018 ◽  
Vol 16 (S1) ◽  
pp. S48-S54
Author(s):  
Y. Ez zoubi ◽  
S. Lairini ◽  
A. Farah ◽  
K. Taghzouti ◽  
A. El Ouali Lalami
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Food Systems ◽  
Antibacterial Activities ◽  
Culture Collection ◽  
Bornyl Acetate ◽  
Bacterial Strains ◽  
Disk Diffusion Assay ◽  
Different Strains

The purpose of this study was to determine the chemical composition and to evaluate the antioxidant and antibacterial effects of the Moroccan Artemisia herba-alba Asso essential oil against foodborne pathogens. The essential oil of Artemisia herba-alba was analyzed by gas chromatography coupled with mass spectroscopy. The antibacterial activity was assessed against three bacterial strains isolated from foodstuff and three bacterial strains referenced by the ATCC (American Type Culture Collection) using the disk diffusion assay and the macrodilution method. The antioxidant activity was evaluated using the DPPH (2, 2-diphenyl-1- picrylhydrazyl) method. The fourteen compounds of the Artemisia herba-alba essential oil were identified; the main components were identified as β-thujone, chrysanthenone, α-terpineol, α-thujone, α-pinene, and bornyl acetate. The results of the antibacterial activity obtained showed a sensitivity of the different strains to Artemisia herba-alba essential oil with an inhibition diameter of 8.50 to 17.00 mm. Concerning the MICs (minimum inhibitory concentrations), the essential oil exhibited much higher antibacterial activity with MIC values of 2.5 μl/ml against Bacillus subtilis ATCC and Lactobacillus sp. The essential oil was found to be active by inhibiting free radicals with an IC50 (concentration of an inhibitor where the response is reduced by half) value of 2.9 μg/ml. These results indicate the possible use of the essential oil on food systems as an effective inhibitor of foodborne pathogens, as a natural antioxidant, and for potential pharmaceutical applications. However, further research is needed in order to determine the toxicity, antibacterial, and antioxidant effects in edible products.

scholarly journals ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES

2017 ◽  
Vol 9 (10) ◽  
pp. 276 ◽  
Author(s):  
Gouse Basha Sheik ◽  
Muazzam Sheriff Maqbul ◽  
Gokul Shankar S. ◽  
Ranjith M S
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Streptomyces Sp ◽  
Crude Extracts ◽  
Test Organisms ◽  
Inhibition Zones

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteriaMethods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods.Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm).Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

scholarly journals Antibacterial and anti-biofilm activities of crude extracts of Lawsonia inermis against Methicillin Resistant Staphylococcus aureus

2016 ◽  
Vol 9 (6) ◽  
pp. 263 ◽  
Author(s):  
Triveni A G ◽  
Suresh Kumar Mendem ◽  
Channapa T Shivannavar ◽  
Subhaschandra M Gaddad
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multi Drug Resistance ◽  
Lawsonia Inermis ◽  
Biofilm Inhibition ◽  
Methicillin Resistant ◽  
Crude Extracts

 Objective:The continuous rise in the prevalence of multi drug resistance pathogens globally is threatening the treatment and management of infectious diseases. Ethno medicine plays a key role in the exploration for novel bioactive compounds. The present study evaluates the antibacterial and antibiofilm activities of the crude extracts of Lawsonia inermis against clinical isolates of methicillin resistant Staphylococcus aureus (MRSA).Materials and methods: Shade dried and finely powdered leaves of the plant were extracted by maceration method using six solvents methanol, acetone, ethyl acetate, chloroform, petroleum ether and n-hexane. Antibacterial and antibiofilm activities of the extracts against MDR MRSA by agar cup diffusion and tube method respectively.Results: Methanol extract showed the highest antibacterial activity of 18mm compared to other extracts. Similarly, petroleum ether extract showed highest biofilm inhibition of 84.7%. Other solvent extracts also exhibited significant biofilm inhibition [n-Hexane-83.6%, Ethyl acetate -79.5%, Chloroform-79.2%, Acetone -77% and Methanol-77%].Conclusion: The leaf extracts of L. inermis have shown promising biofilm inhibitory activity and good antibacterial activity, which can be explored for the development of new drugs for the MDR pathogens. Keywords: Antibioticacivity, Antibiofilm activity, L.inermis, MRSA

scholarly journals ANTIBACTERIAL ACTIVITY OF METHANOLIC RHIZOME EXTRACT OF ALPINIA CALCARATA ROSC. (ZINGIBERACEAE)

2016 ◽  
Vol 3 (1) ◽  
pp. 65-66
Author(s):  
Arumugasamy K ◽  
Nantha Kumar R ◽  
Abdul Kaffoor H ◽  
Shalimol A
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Bacterial Activity ◽  
Proteus Vulgaris ◽  
Bacterial Strains ◽  
Zone Of Inhibition

The methanolic rhizome extract of A. calcarata was evaluated for its antibacterial activities against five bacterial strains Pseudomonas aeuroginosa, Proteus vulgaris, Salmonella paratyphi, Bacillus thurungiensis and Staphylococcus faccealis. The extract has inhibited all the tested bacterial species with different manner at various concentration. However the higher level zone of inhibition in 400 (mg/ml) is significant against all the above said bacterial strains of these Salmonella paratyphi. Based on the present study it can be conculuded that the plant rhizome possess potent anti bacterial activity.

Synthesis and Antibacterial Activities of Amidine Substituted Monocyclic β-Lactams

2021 ◽  
Vol 17 ◽  
Author(s):  
Lijuan Zhai ◽  
Lili He ◽  
Yuanbai Liu ◽  
Ko Ko Myo ◽  
Zafar Iqbal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Species ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Lead Target ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Clinical Interest

Background: Mononcyclic β-lactams are regarded as the most resistant class of β-lactams against a series of β-lactamases though possess limited antibacterial activity. Aztreonam being the first clinically approved monobactam needs broad-spectrum efficacy through structural modification. Objective: We strive to synthesize a number of monocyclic β-lactams by varying the substituents at N1, C3 and C4 positions of azetidinone ring and study the antimicrobial effect on variable bacterial strains. Methods: Seven new monobactam derivatives 23a-g, containing substituted-amidine moieties linked to the azetidinone ring via thiazole linker, were synthesized through multistep synthesis. The final compounds were investigated for their in vitro antibacterial activities using broth microdilution method, against ten bacterial strains of clinical interest. The minimum inhibitory concentrations (MICs) of newly synthesized derivatives were compared with aztreonam, ceftazidime and meropenem, existing clinical antibiotics. Results: All compounds 23a-g showed higher antibacterial activities (MIC 0.25 µg/mL to 64 µg/mL) against tested strains as compared to aztreonam (MIC 16 µg/mL to >64 µg/mL) and ceftazidime (MIC >64 µg/mL). However all compounds, except 23d, exhibited lower antibacterial activity against all tested bacterial strains as compared to meropenem. Conclusion: Compound 23d showed comparable or improved antibacterial activity (MIC 0.25 µg/mL to 2 µg/mL) to meropenem (MIC 1 µg/mL to 2 µg/mL) in case of seven bacterial species. Therefore, compound 23d may be valuable lead target for further investigations against multi-drug resistant Gram-negative bacteria.

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

Sign in / Sign up

Export Citation Format

Share Document