scholarly journals PEMANFAATAN PESTISIDA NABATI PADA PENGENDALIAN HAMA PLUTELLA XYLOSTELLA TANAMAN SAWI (Brassica juncea L.) MENUJU PERTANIAN RAMAH LINGKUNGAN

2017 ◽  
Vol 6 (1) ◽  
pp. 36
Author(s):  
Suhartini Suhartini ◽  
Putu Suryadarma ◽  
Budiwati Budiwati
Keyword(s):  
Nicotiana Tabacum ◽  
Brassica Juncea ◽  
Plutella Xylostella ◽  
Positive Control ◽  
Negative Control ◽  
Leaf Damage ◽  
Piper Betle ◽  
Randomized Design ◽  
Biological Pesticide ◽  
Chemical Pesticides

Abstrak Penggunaan pestisida kimia telah banyak memberikan dampak negatif pada lingkungan, sehingga diperlukan penggunaan pestisida nabati untuk menuju pertanian yang ramah lingkungan. Di desa ditemui banyak jenis daun yang dapat digunakan sebagai pestisida hayati, maka dari itu penelitian ini bertujuan untuk mengetahui efektivitas dari ekstrak pestisida nabati yaitu daun tembakau, tapak liman, daun kayu kuning dan daun sirih hijau terhadap mortalitas hama Plutella Xylostella pada tanaman sawi (Brassica juncea L.) berat basah sawi dan kerusakan daun sawi.Penelitian ini menggunakan Rancangan Acak Lengkap dengan perlakuan jenis tanaman sebagai pestisida Nabati . Perlakuan yang digunakan ada 6 macam (taraf) yaitu kontrol negatif (P0), daun tembakau (P1), tapak liman (P2), daun kayu kuning (P3),  daun sirih hijau (P4) dan pestisida kimia sebagai kontrol positif (P5) dengan masing-masing kadar 10 %. Parameter yang diamati adalah mortalitas hama,  berat basah sawi dan tingkat kerusakan daun sawi. Analisis dilakukan dengan anova satu arah.Hasil penelitian menunjukkan ekstrak daun tembakau ((Nicotiana tabacum), tapak liman ((Elephantopus sp.), daun kayu kuning (Arcangelisia flava L.) dan daun sirih hijau  (Piper betle Linn.) dengan konsentrasi 10 persen dari ekstrak belum memberikan pengaruh nyata pada mortalitas hama Plutella xylostella, berat basah sawi ((brassica juncea l.)  dan tingkat kerusakan daun sawi (brassica juncea l.). Ekstrak daun yang paling berpengaruh pada mortalitas Plutella xylostella secara berurutan adalah tembakau, daun sirih, daun kayu kuning dan tapak liman. Sedangkan pada berat basah sawi (brassica juncea l.) secara berurutan daun kayu kuning, daun sirih hijau, daun tapak liman dan daun tembakau. Sementara terhadap tingkat kerusakan daun sawi mulai yang paling kecil rusaknya  secara berurutan adalah daun sirih hijau, daun tembakau, tapak liman dan daun kayu kuning. Kata Kunci: Pestisida Nabati, ekstrak, Plutella xylostella, mortalitas, sawi (Brassica juncea) ABSTRACTThe use of chemical pesticides has many negative impacts on the environment, it is necessary for the use of biological pesticide towards sustainable agriculture or environmentally friendly agriculture. In the village were encountered many types of leaves that can be used as a biological pesticide, and therefore this study aims to determine the effectiveness of pesticide plant extracts of the leaves of some plants covering the leaves of tobacco, elephantopus, yellow wood and green betel on mortality of Plutella xylostella pests in plants mustard greens (Brassica juncea L), heavy wet mustard and mustard leaf damage   This study uses a completely randomized design with the treatment of various crops as a pesticide vegetable. The treatments used 6 kinds (degree) ie negative control (P0), the leaves of tobacco (P1), the leaves of elephantopus (P2), the leaves of yellow wood (P3), the leaves of greens betel (P4) and chemical pesticides as a positive control (P5) with each grade of 10 %. Parameters measured were mortality pests, heavy wet mustard greens and mustard greens leaf damage rate. The analysis was performed by one-way ANOVA.   The results showed that the extract from the leaves of tobacco (Nicotiana tabacum), the leaves of elephantopus, the leaves of yellow wood (Arcangelisia flava L.) and the leaves of green betel (Piper betle Linn.) With a 10 percent concentration of the extract on a variety of  leaves are not yet significant effect on mortality pests Plutella xylostella, heavy wet of mustard greens (brassica juncea l.) and severity of leaf mustard greens (brassica juncea l.). The leaf extract the most influence on mortality Plutella xylostella sequential is an extract of the leaves tobacco, the leaves of greens betel, the leaves of yellow wood and the leaves of elephantopus. While the effect on weight of wet mustard greens (Brassica juncea l.) in order are as follows: the leaves of yellow wood, the leaves of green betel, the leaves of elephantopus, and the leaves of tobacco. Meanwhile the level of damage to the mustard greens leaves are starting from the smallest breakdown in order are the leaves of green betel, the leaves of tobacco, the leaves of elephantopus and the leaves of yellow wood. Keywords: Pesticides Vegetable, extract, Plutella xylostella, mortality, Brassica juncea 

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Randomized Design ◽  
Decrease Blood Glucose ◽  
Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

scholarly journals Penggunaan Kulit Nanas Fermentasi dalam Ransum yang Mengandung Gulma Berkhasiat Obat Terhadap Konsumsi Nutrient Ayam Broiler

2016 ◽  
Vol 16 (2) ◽  
pp. 76
Author(s):  
Wasir Ibrahim ◽  
Rita Mutia ◽  
Nurhayati Nurhayati ◽  
Nelwida Nelwida ◽  
Berliana Berliana
Keyword(s):  
Nutrient Intake ◽  
Broiler Chicken ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Pineapple Peel ◽  
Nutrient Consumption ◽  
Before And After ◽  
Completely Randomized Design ◽  
And Performance

ABSTRAK. Penelitian ini bertujuan untuk mengetahui penggunaan kulit nanas fermentasi ke dalam ransum yang mengandung gulma berkhasiat obat terhadap konsumsi nutrient ayam broiler. Penelitian ini menggunakan DOC jantan strain cob umur 2 hari sebanyak 200 ekor yang dipelihara selama 42 hari. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. P0 = 0% tepung kulit nanas fermentasi (TKNF) dalam ransum mengandung 0% gulma obat(GO), (kontrol positif), P1 = 0% TKNF dalam ransum mengandung 2 % gulma obat (GO),(kontrol negatif), P2 = 7.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P3 = 15% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat, P4 = 22.5% TKNF dalam ransum mengandung 2 % gulma berkhasiat obat. Parameter yang diamati kandungan gizi kulit nanas sebelum dan sesudah fermentasi dan konsumsi nutrient. Pengaruh yang nyata terhadap parameter yang diamati dilanjutkan dengan Uji Jarak Berganda Duncan, Data yang diperoleh dari setiap parameter dianalisis ragam (ANOVA) menggunakan bantuan software SPSS 16.0. Dari hasil penelitian disimpulkan bahwa penggunaan kulit nanas yang fermentasi dengan yoghurt dalam ransum yang mengandung gulma berkhasiat obat pada taraf 15% memberikan hasil terbaik pada konsumsi nutrient ayam broiler. (Fermented pineapple peel supplementation with addition of medicinal weeds on nutrient intake consumption of broiler chicken) ABSTRACT. The aim of this study was to determine the effect of using fermented pineapple peel in the ration containing medicinal weeds on nutrient consumption and performance of broiler . Two hundred 2 days male broiler chicken cob strain were used in this study and kept for 42 days. Completely randomized design (CRD)was applied with 5 treatments and 5 replicates. Treatments consisted of P0 = 0% of fermented pineapple peels meal (FPPM) in ration containing 0% medicinal weed (MW), positive control). P1 = 0% of fermented pineapple peels meal (FPPM) in the ration containing 2 % medicinal weed (MW), negative control). P2 = 7.5% of FPPM in the ration containing 2 % medicinal weed (MW). P3 = 15% of FPPM in the ration containing 2 % medicinal weed (MW). P4 = 22,5% of FPPM in the ration containing 2 % medicinal weed (MW). The parameters measured was nutrien intake before and after fermented peel and consumption. The observed data were analyzed by statistical product and service solution (SPSS 16.0). Results showed that use of fermented pineapple peel meal up to 15% with addition medicinal weeds was the best on nutrient consumption of broiler chicken.

scholarly journals Blood glucose and total cholesterol content of hyperglycemic white male rat (Rattus norvegicus L.) after orally intakes of methanol meniran (Phyllanthus niruri L.) root extract

2005 ◽  
Vol 3 (1) ◽  
pp. 1-6 ◽  
Author(s):  
CHASBI FAHRI ◽  
SUTARNO SUTARNO ◽  
SHANTI LISTYAWATI
Keyword(s):  
Blood Glucose ◽  
Total Cholesterol ◽  
White Male ◽  
Cholesterol Content ◽  
Positive Control ◽  
Negative Control ◽  
Male Rat ◽  
Root Extract ◽  
Phyllanthus Niruri ◽  
Randomized Design

The aims of this research were to study the effect of methanol meniran (Phyllanthus niruri L.) root extract given to the blood glucose and total cholesterol content, and which level of concentration giving significant effect alloxan treatment. Meniran root contains ellagic acid as antioxidant which provides hypoglycemic capability to reduce diabetic blood glucose. This research was done by using completely randomized design (CRD) including eight treatments as follow: negative control (CMC 1%, 2 mL/200 g bw), positive control (glibenclamide 0,126 mg/200 g bw), normal control, meniran root extract in various concentration (2; 4; 6; 8; 10 mg/200 g bw). Data were elucidated until 15 day of treatment and analyzed using ANOVA followed by DMRT at 5% confidence level. The result indicated that meniran root extract giving significant effect on the reduction of blood glucose, however, it does not appear to have the same result to total cholesterol content. At various concentration of meniran root extract, the total cholesterol of rat remains stable. The optimum concentration to provide hypoglycemic activity raised at 10 mg/200 b bw dose. 

scholarly journals Probiotic and synbiotic in broiler diet: performance and Enterobacteriaceae

2020 ◽  
Vol 72 (6) ◽  
pp. 2365-2372
Author(s):  
P.R.S.C. Leite ◽  
H.B. Oliveira ◽  
V.B.L. Souza ◽  
F.O. Rocha ◽  
T.H. Oliveira
Keyword(s):  
Broiler Chickens ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Broiler Performance ◽  
Randomized Design ◽  
Colony Forming Units ◽  
Completely Randomized Design ◽  
Zinc Bacitracin ◽  
Experimental Treatments

ABSTRACT The objective of this study was to evaluate the effects of probiotics and synbiotics on the performance and Enterobacteriaceae count of broiler chickens. A total of 640 one-day-old male broiler chicks were distributed in a completely randomized design with four treatments and eight replicates with 20 birds each. The treatments were: ration with performance enhancer (zinc bacitracin; positive control); ration without performance enhancer and probiotic/synbiotic (negative control); ration with probiotics; and ration with synbiotics. At 35 days, five birds from each treatment were euthanized and intestinal contents were harvested for determining the Enterobacteriaceae count. The performance data and average colony-forming units (CFUs) transformed as log CFU/g were subjected to analysis of variance and Tukey’s test. The effects of probiotics and synbiotics were observed in the initial phase, with supplemented birds exhibiting comparable weight gain to those supplemented with bacitracin. No effect of the treatment on broiler performance was observed after 42 days. The enterobacterial count was comparable among all experimental treatments. Supplementation with probiotics and synbiotics did not compromise the performance of broilers and did not alter the Enterobacteriaceae count.

scholarly journals Effects of alphamune G on the performance, serum and haematological parameters of Escherichia coli challenged turkey poults

2020 ◽  
Vol 40 (2) ◽  
pp. 112-121
Author(s):  
S. A. Bolu ◽  
M. T. Adelakun
Keyword(s):  
Escherichia Coli ◽  
Poor Performance ◽  
Positive Control ◽  
Negative Control ◽  
E Coli ◽  
Turkey Poults ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Improved Performance ◽  
Haematological Indices

A study was conducted to determine the response of Turkey poults to graded levels of Alphamune G (0.00+, 0.03, 0.04, 0.05, 0.06 and 0.00 %-) when challenged with Escherichia coli orally for 7 days. The graded levels were the treatments viz 0.00%+ (positive control), Alphamune G at 0.03, 0.04, 0.05, 0.06% and 0.00%- (negative control; infected without Alphamune G supplementation). Each treatment was allotted 3 replicates of 6 poults. The experiment which was conducted for 56 days employed a completely randomized design. E. coli was isolated from the intestinal digesta of a colisepticaemic chicken. 108 turkey poults were used in this study. Poults were infected with E.coli for 7 days through the drinking water and given the treatment. The performance parameters of Alphamune G supplementation were significantly affected. The cumulative weight, Feed intake and weight gain were highest for turkey poults fed 0.06% Alphamune G supplementation. These values were also directly proportional to the supplementation levels of Alphamune G. The birds given the negative treatment (0.00 %-) had relatively poor performance compared to the other treatments. The specific enzymes studied were significantly affected (p<0.05) by the treatments. ALT and AST were significantly highest for turkey poults fed the negative control. Enzyme values became optimum at 0.05% Alphamune G supplementation. At 0.06% of Alphamune G supplementation, cellular mitigations of the effects of E. coli was measurable. Urea and creatinine were not significantly (p>0.05) influenced by the treatments. Haematological indices such as WBC and specific differential counts (lymphocytes and neutrophils) were affected significantly (P<0.05) by supplemental levels of Alphamune G The Inclusion of Alphamune G at 0.06% in the diets improved performance of turkey poults when challenged with Escherichia coli.

scholarly journals Effect of multienzymes + probiotic supplementation in fermented shea butter cake based diets on the carcass traits and the biochemistry of broiler chickens

2020 ◽  
Vol 12 (3) ◽  
pp. 227-233
Author(s):  
P.C. Aguihe ◽  
A.S. Kehinde ◽  
S.K. Halidu ◽  
A. Sulyman ◽  
J. Chikezie ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Serum Biochemistry ◽  
Positive Control ◽  
Serum Glucose ◽  
Negative Control ◽  
Shea Butter ◽  
Broiler Chicks ◽  
Breast Meat ◽  
Randomized Design

Abstract. This study aimed to evaluate the effect of multienzyme+probiotic (MEP) supplementation on carcass characteristics and serum biochemistry of broiler chickens fed fermented shea butter cake meal (FSBCM) based diets. A total of two hundred and sixty (260), 28-day-old broiler chicks were distributed into four treatments, with five replicates of 13 birds each using a completely randomized design and reared on deep litter system from 28 to 56 days of age. The four treatments were: positive control – T1 (corn-soybean based diet); negative control – T2 (20% FSBCM replacement for corn in T1); diet 3 – T3 (T2 diet + 0.05% MEP) and diet 4 – T4 (T2 diet + 0.10% MEP). On day 56, two birds per each pen were selected for carcass and organ evaluation and other two birds per replicate were selected for serum biochemical analysis. The results indicated that addition of MEP to FSBCM diet caused a significant (p<0.05) increase in live weight, dressing percentage and breast meat yield than FSBCM diet without MEP and did not differ from the T1 diet. The abdominal fat in birds fed MEP treated diets were significantly (p<0.05) lower than those groups fed PC and FSBCM diets. Serum glucose was similar (p>0.05) between birds fed PC and MEP treated diets but significantly (p<0.05) higher than those on FSBCM diet, whereas serum cholesterol, alanine aminotransferase and aspartate aminotransferase concentrations reduced significantly (p<0.05) in groups fed MEP supplemented FSBCM diets. All other parameters were not significantly (p>0.05) affected by the MEP supplementation. Therefore, MEP supplementation in FSBCM based diet improved live weights and carcass attributes without any detrimental effect on blood composition of the broiler chickens.

scholarly journals Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Sadakata Sinulingga ◽  
Subandrate Subandrate ◽  
Bebbi Arisya Kesumaputri ◽  
Galuh Anggraini
Keyword(s):  
Bleeding Time ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Piper Betle ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Group Design ◽  
Hemostatic Effect

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Mice’s tail was cut at diameter 3 mm and pretest bleeding time was counted. Mice’s tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

scholarly journals Aktivitas Antifungi Ekstrak Etanol Daun Eryngium foetidum L. Terhadap Pertumbuhan Xeromyces sp. secara in vitro

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Ines S Simatupang Elvi Rusmiyanto PW, Rikhsan Kurniatuhadi
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Level ◽  
Piper Nigrum ◽  
Dilution Method ◽  
Randomized Design ◽  
Eryngium Foetidum ◽  
Completely Randomized Design

Eryngium foetidum is a plant commonly used a seasoning cuisine and has a variety of chemical compounds that potentially an antifungal. The ethanol extract Eryngium foetidum was tested against fungal isolate Xi.01. The isolat Xi.01 isolated from the pepper (Piper Nigrum) stem was identified as Xeromyces sp. This study aimed to determine the ability of the antifungal ethanol extract of Eryngium foetidum against Xeromyces sp. (Xi.01). This study used solid dilution method and completely randomized design using 18 treatments, i.e negative control, positive control, diethanolamide concentration of 2.5; 5; 7.5 and 10% combined with the ethanol extract concentration of Eryngium foetidum of 5; 10; 20 and 40%. The results showed 16 treatment combinations had the same low antifungal activity level in the range 13,59-22,40%

scholarly journals Antibacterial activities of combination of ethanol extract of Delima fruit (Punica granatum L.) and Sereh stem (Cymbopogon citratus) on Staphylococcus aureus

2019 ◽  
Vol 6 (12) ◽  
pp. 5037
Author(s):  
Melia Pebrina ◽  
Indah Komala Sari ◽  
Eliza Arman ◽  
Honesty Diana Morika
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Pomegranate Peel Extract

ABSTRACT Background: Pomegranates contain chemical saponins and flavonoids while lemongrass stems have flavonoid chemical compounds. Substances that can inhibit the linking of bacteria are saponins and flavonoids. The most common microorganism found in ulcus diabetikum (ulcer diabetes) is Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of a combination of pomegranate ethanol extract and ethanol extract of lemongrass stems against S. aureus bacteria.Methods: This study used an experimental design with a complete randomized design study divided into 5 groups: groups 1 (75:25), 2 (50:50), 3 (25:75), positive control (tetracycline) and negative control. Manufacture of pomegranate peel extract and lemongrass stems was done by maceration method for further rotary, after the extract was obtained tested for antibacterial activity by diffusion method using a cylinder. With the test used analysis of variance one way.Results: The results of this study showed that the combination of pomegranate ethanol extract and citronella stem ethanol extract showed activity against S. aureus with a ratio of 15 ul:5 ul, 15 ul:15 ul, 5 ul:15 ul with inhibitory diameter of  respectively 13 mm, 11 mm, 8 mm. Whereas for positive control with chloramphenicol, the inhibition area is 9 mm.Conclusions: Statistically the combination of pomegranate ethanol extract and lemongrass stems has antibacterial power which uses p=0.005 and p<0.05. The combination of pomegranate ethanol extract and lemongrass stems has very strong antibacterial activity against S. aureus due to inhibition zones of 10-20 mm.

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