Blood glucose and total cholesterol content of hyperglycemic white male rat (Rattus norvegicus L.) after orally intakes of methanol meniran (Phyllanthus niruri L.) root extract

The aims of this research were to study the effect of methanol meniran (Phyllanthus niruri L.) root extract given to the blood glucose and total cholesterol content, and which level of concentration giving significant effect alloxan treatment. Meniran root contains ellagic acid as antioxidant which provides hypoglycemic capability to reduce diabetic blood glucose. This research was done by using completely randomized design (CRD) including eight treatments as follow: negative control (CMC 1%, 2 mL/200 g bw), positive control (glibenclamide 0,126 mg/200 g bw), normal control, meniran root extract in various concentration (2; 4; 6; 8; 10 mg/200 g bw). Data were elucidated until 15 day of treatment and analyzed using ANOVA followed by DMRT at 5% confidence level. The result indicated that meniran root extract giving significant effect on the reduction of blood glucose, however, it does not appear to have the same result to total cholesterol content. At various concentration of meniran root extract, the total cholesterol of rat remains stable. The optimum concentration to provide hypoglycemic activity raised at 10 mg/200 b bw dose.

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UJI AKTIVITAS DIURETIK EKSTRAK AIR TANAMAN SELEDRI (Apium graviolens L.) PADA TIKUS PUTIH JANTAN

Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan ◽

10.35960/vm.v10i1.399 ◽

2018 ◽

Vol 10 (1) ◽

pp. 43-46

Author(s):

Adita Silvia Fitriana ◽

Dina Febrina ◽

Galih Samodra ◽

Adita Silvia Fitriana ◽

Dina Febrina ◽

...

Keyword(s):

White Male ◽

Urine Volume ◽

Water Extract ◽

Positive Control ◽

Negative Control ◽

Male Rat ◽

Preparation Process ◽

Diuretic Effect ◽

Made In

Processing of celery as diuretic is done by society in various ways. So far, the most effective preparation process of celery extract has not been known. This research aims to examine the diuretic effect of various celery extract preparation. This research was conducted using celery water extract which is made in accordance with the peopleâ€™s habit, using aquades as a negative control and HCT as a positive control. The effectiveness test of celery water extract as diuretic was done on white male rat by measuring the urine volume every hour for six hours. The results showed that the largest urine volume is obtained from the boiling celery extract.

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EKSTRAK AIR TANAMAN SELEDRI (APIUM GRAVEOLENS) TERHADAP PENINGKATAN VOLUME URIN TIKUS PUTIH

Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan ◽

10.35960/vm.v10i3.439 ◽

2019 ◽

Vol 10 (3) ◽

pp. 1-6

Author(s):

Adita Silvia Fitriana

Keyword(s):

White Male ◽

Urine Volume ◽

Water Extract ◽

Positive Control ◽

Negative Control ◽

Apium Graveolens ◽

Male Rat ◽

Preparation Process ◽

Diuretic Effect ◽

Made In

ABSTRACT Processing of celery as diuretic is done by society in various ways. So far, the most effective preparation process of celery extract has not been known. This research aims to examine the diuretic effect of various celery extract preparation. This research was conducted using celery water extract which is made in accordance with the people’s habit, using aquades as a negative control and HCT as a positive control. The effectiveness test of celery water extract as diuretic was done on white male rat by measuring the urine volume every hour for six hours. The results showed that the largest urine volume is obtained from the boiling celery extract. Keywords: celery, diuretic, rat

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Uji Potensi Nefropati Diabetes Daun Sirih Merah (Piper croatum Ruiz & Pav) pada Tikus Putih Jantan (Rattus norvegicus)

KOVALEN ◽

10.22487/kovalen.2020.v6.i3.15323 ◽

2020 ◽

Vol 6 (3) ◽

pp. 239-251

Author(s):

Joni Tandi ◽

Rizaldy Lalu ◽

Magfirah ◽

Yunlis Silintowe Kenta ◽

Ronaldy Nobertson

Keyword(s):

Blood Glucose ◽

White Male ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Average Dose ◽

Average Value ◽

Betel Leaf ◽

White Rats

This study aims to determine the type of secondary metabolite compounds in red betel leaf ethanol extract, the potential of red betel leaf ethanol extract on blood glucose, creatinine, and urea levels of male white rats induced by streptozotocin. This study used 30 white male rats which were divided into 6 groups: normal control, negative control, positive control, the dose of 150, 250, and 350 mg/kg BW. The showed that results ethanol extract of red betel leaves contained alkaloids, flavonoids, phenols, saponins, and tannins. Ethanol extract of red betel leaf at a dose of 150 mg /kg BW is an effective dose in reducing levels glucose blood with an average value of decreased blood glucose levels of 238.25 mg/dL, and has an effect on creatinine and ureum, with an average dose of 350 mg/kg BW with a mean creatinine level of 0.64 mg/dL and mean ureum of 39.68 mg/dL. Keywords: Creatinine, ureum, red betel leaf, streptozotocin

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PENGARUH FRAKSI AIR DAUN KEDONDONG BANGKOK (Spondias dulcis) TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT PUTIH JANTAN

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v7i2.55 ◽

2019 ◽

Author(s):

Ramadhan Triyandi ◽

Nopiyansyah Nopiyansyah ◽

Robi Wira Pratama

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

White Male ◽

Positive Control ◽

Negative Control ◽

Premature Aging ◽

Male Mice ◽

Water Fraction ◽

Average Value

Ambarella Bangkok fruit (Spondias dulcis) is fruits from Anacardiaceae family. Ambarella plant is known containing secondary metabolite compounds such as alkaloid, flavonoid, saponin and tannin. Flavonoid is a polyphenol compound which work as antioksdant. Ambarela Bangkok leaf containing rich antioksidant which can be use as prevention of cancer, heart disease, cholesterol, diabetes, and premature aging. The purpose of this research is to look effect from water fraction from Ambarela leaf (spondias dulcis) to decrease diabetes disease from white male mice. In this research ambarela leaf is extracted by maseration with 70% alcohol. After the extract obtained then fractionation prosen be held. From the fraction wich ocurred, then made three variant of dose those are 175.5 mg/kgW, 351 mg/kgW and 702 mg/kgW. From those three dose, the 702mg/ kgW dose has the best effect from another dose. Positive control used glibenclamide and negative control used aquadest. The result of this research shows that water fraction from Ambarela leaf can decrease diabetes desease from the white male mice. The the greater fraction concentration, the greater the concentration of the fraction then the decrease of blood glucose level in mice is getting better. The 702mg/kgW dose has an average value of decline 94.3 mg/Dl. The result of analisys shows that watter fraction of ambarella Bangkok leaf can decrease the blood glucose level in male white mice. Key word : Spondias dulcis, water fraction of Ambarela leaf , Blood glucose level

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PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

BioScience ◽

10.24036/02018219965-0-00 ◽

2018 ◽

Vol 2 (1) ◽

pp. 61

Author(s):

Elsa Yuniarti

Keyword(s):

Blood Glucose ◽

Mus Musculus ◽

Leaf Extract ◽

Negative Control ◽

State University ◽

Betel Leaf ◽

Randomized Design ◽

The Subject ◽

Effective Drugs ◽

Zoology Department

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

Jurnal Katalisator ◽

10.22216/jk.v3i1.2178 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

Verawaty Verawaty ◽

Dhea Claudia Novel

Keyword(s):

Blood Glucose ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

The Third ◽

Blood Glucose Levels

Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.

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UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽

10.35799/pha.10.2021.32767 ◽

2021 ◽

Vol 10 (1) ◽

pp. 736

Author(s):

Grace Laury Tulung ◽

Widdhi Bodhi ◽

Jainer Pasca Siampa

Keyword(s):

Rattus Norvegicus ◽

Design Method ◽

Centella Asiatica ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Flavonoid Compound ◽

Randomized Design ◽

Decrease Blood Glucose ◽

Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords: Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus ABSTRAK Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

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UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

Jurnal Insan Farmasi Indonesia ◽

10.36387/jifi.v4i1.602 ◽

2021 ◽

Vol 4 (1) ◽

pp. 44-53

Author(s):

Andri Priyoherianto ◽

◽

Panji Ratih Suci ◽

Putri Rizki Cahya Fatimah ◽

Atik Nur Wijayanti

Keyword(s):

Blood Glucose ◽

Blood Sugar ◽

Water Hyacinth ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Glucose Levels ◽

Normal Limits ◽

Blood Glucose Levels ◽

Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

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AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v7i2.56 ◽

2019 ◽

Author(s):

Haka As'ada ◽

Yardi Saibi ◽

Hendri Aldrat

Keyword(s):

Dose Range ◽

White Male ◽

Ethanol Extract ◽

Negative Control ◽

Inflammatory Activity ◽

Male Rat ◽

Control Group ◽

Angelica Keiskei ◽

Anti Inflammatory

Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.

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