Influence of Retained Fetal Membranes on Reproductive Health of Crossbred Cows

Author(s):  
K. Rokde ◽  
S. Kumar ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
R. Aich ◽  
...  

A study was carried out to compare the effect of retained fetal membranes on reproductive health of crossbred cows. 24 crossbred cows selected were divided into control group (consisted of 6 cows which had normal 3rd phase of parturition) and RFM group (consisted of total 18 cows with retained fetal membranes for more than 12 hrs). The time required for expulsion fetal membranes, cessation of lochial discharge, gross uterine involution, service period and number of AI required per conception was significantly (P less than 0.05) higher and appearance of first postpartum estrus was nonsignificantly higher in retained fetal membranes (RFM) cows than normally calved cows.

Author(s):  
A. J. Dhami ◽  
V. K. Theodore ◽  
M. T. Panchal ◽  
K. K. Hadiya ◽  
P. M. Theodore ◽  
...  

Advanced pregnant healthy HF crossbred cows (n=20) of 2-4 parity were equally divided in to control (routine farm feeding-RFF) and treatment/nutrients supplementation (RFF + bypass fat @ 100-200 g/h/d and ASMM @ 50 g/h/d) groups and were studied from 2 wks prepartum to 8 wks postpartum for plasma profile of steroid hormones and metabolites on days -14, -3, 0, +3, +14, +28 and +42 as well as for puerperal events and postpartum fertility. The mean plasma progesterone values were maximum (>6 ng/ml) on day 14 prepartum, which declined significantly (p>0.01) on day 3 prepartum reached to the basal levels (>1 ng/ml) on the day of calving, remained basal till day 14, and thereafter showed a rising trend on days 28 and 42 postpartum. The oestradiol-17b values were at its peak on the day of calving (p>0.01), showed a rapid fall by day 3 postpartum and remained low till recrudesce of follicular activity around day 35 postpartum. However, there were no statistical differences between the two groups in either of the hormones, except on day 42 postpartum. The levels of cortisol and PGF2 a Metabolites (PGFM) were 3-8 times higher on the day of parturition as compared to values at day 14 pre- and postpartum, and declined further till day 42 postpartum reaching to prepartum levels. The plasma cholesterol gradually decreased as parturition approached and increased in postpartum days to reach the highest value (p>0.01) at day 42. The nutrients supplemented cows had significantly (p>0.01) higher plasma cortisol, PGFM and cholesterol values than the control cows around parturition. However, no such variation was noted in plasma protein profile. The period of uterine involution in control and supplemented groups was identical (31.97±1.82 and 30.27±1.41 days), yet the cows in treatment group resumed estrous cycle earlier (38.00±1.95 vs 42.32±4.14 days, p>0.05) and had shorter service period (85.22±7.17 vs 100.67±5.60 days) with improved pregnancy rate (80 vs 60 %) as compared to those in control group. Thus, the peripartum nutrient supplementation in crossbred cows was beneficial and had positive effect on the postpartum fertility and plasma cortisol, PGFM and cholesterol profile.


Author(s):  
G. B. Solanki ◽  
J. K. Chaudhary ◽  
H. P. Vijyeta ◽  
P. M. Gamit ◽  
S. S. Parikh

The therapeutic efficacy of methylergometrine and PGF2α on uterine involution and postpartum fertility was studied in 18 (6/group) Gir cows. Animals of groups I and II were treated with single i/m injection of PGF2α and Methylergometrine immediately after parturition, respectively, while group-III served as untreated control. The time required for expulsion of fetal membranes was shorter in group-I (2.55 ± 0.60 hours) than in group II (3.03 ± 0.34 hours) and group III (4.10 ± 0.36 hours) but did not differ statistically. Time taken for completion of uterine involution was significantly (p Less than 0.05) shorter in group I (28.67±1.12 days) than that of group II (35.83 ± 1.49 days) and group-III (41.00±1.46 days). The intervals for first estrus postpartum and service period were also significantly (p Less than 0.01) shorter for group I (37.83 ± 1.10 and 89.50 ± 4.34 days) than in group-II (46.17 ± 1.40 and 108.33 ± 4.91 days) and group-III (50.83 ± 1.11 and 118.33 ± 4.40 days, respectively). The number of services per conception was non-significantly lower in group I (1.17 ± 0.17) as compared to group II (1.50 ± 0.34) and controlled group III (1.67±0.33). The conception rate was 100% in group I and 83.33 % each in group II and III. Thus, it can be concluded that use of PGF2α immediately after parturition in Gir cows enhanced the process of placental separation, hastened the uterine involution, decreased the service period, increased the conception rate and thereby reduce the calving interval to the profitable ambiance as compared to Methylergometrine and control groups.


Author(s):  
A. I. Shah ◽  
D. M. Patel ◽  
N. P. Sarvaiya ◽  
S. P. Madhira

This study was undertaken on 36 freshly calved cows randomly divided into 6 equal groups under field conditions. Cows of group-VI that shed placenta within 8-12 hours postpartum naturally served as healthy control. The cows with retained fetal membranes (RFM, n = 18) for more than 12 hrs were managed either by manual removal of placenta without antibiotics (group-I), parenteral antibiotic (Ceftiofur 1 g i/m) for three consecutive days (group-II) or a combination of both (group-III). In group-IV and group-V, cows were administered with Inj. Oxytocin @ 50 IU i/m and Inj. Dinoprost tromethamine (PGF2α) @ 25 mg i/m, respectively, immediately after parturition and time of placental shedding was recorded. The overall prevalence of Brucellosis by RBPT was found to be 5.55 % amongst these 36 animals. The placental expulsion in groups following medicinal treatment was found to be 50 (3/6) % in Ceftiofur alone by 3 days (group-II), and 66.67 (4/6) % in Oxytocin (group-IV) and 100 (6/6) % in PGF2α inj. (group-V) groups within 12 hrs. The time of uterine involution in groups I to VI was found to be 42.00 ± 1.94, 39.50 ± 0.99, 40.67 ± 1.39, 38.33 ± 1.55, 37.50 ± 1.02 and 37.33 ± 1.76 days, respectively, while the interval for the appearance of first postpartum estrus was 54.83 ± 2.06, 51.00 ± 1.05, 52.17 ± 1.96, 50.17 ± 2.03, 48.67 ± 1.90 and 49.17 ± 1.55 days, respectively, which did not vary statistically. The mean serum progesterone profile obtained on day 0 and day 21 postpartum was statistically non-significant between groups. However, it was significantly (p less than 0.05) lower on day 0 as compared to day 21 in group-I, II and VI. The levels on day 0 coincided with the time of blood sampling after calving. The high level of serum P4 on day 0 in group-IV and V could be due to sampling immediately after calving. The serum calcium and phosphorus levels were significantly(p less than 0.05) lower on day 0 than on day 21, but not the magnesium. The group effect was however non-significant for any of three minerals. It was observed that manual removal of RFM without parenteral antibiotics, resulted in puerperal metritis, cervicitis, pyometra which ultimately resulted into delayed uterine involution, delayed first postpartum estrus and thus, reduced the postpartum reproductive efficiency. It was inferred that the PGF2α and Oxytocin injections could be used as a treatment of choice for prevention of RFMs in cattle.


Author(s):  
K. B. Vala ◽  
A. J. Dhami ◽  
F. S. Kavani ◽  
S. C. Parmar ◽  
M. M. Pathan

This investigation was undertaken on 40 transitional Jaffarabadi buffaloes with the objective to evaluate the effect of peripartum nutritional (multiminerals and bypass fat) supplementation without and with micro-minerals (Inj. Stimvet) and oral ecbolic (Boli Exapar) on uterine involution and postpartum fertility including plasma minerals profile. The animals of treatment group (n=20) received additional oral supplements daily with 50 g of chelated mineral mixture and 150 g of bypass fat along with concentrates for 6 weeks prepartum and 2 weeks postpartum over the control farm fed group (n=20). Ten buffaloes in each control and treatment group further received Inj. Stimvet 5 ml (micro-minerals) around day 45 prepartum and on day of calving. Half of these Stimvet treated and control subgroups also received Exapar (n=5) 2 boli/day for 4 consecutive days postpartum. Blood samples were obtained on day –45, –30, –7 and 0 (day of calving), 7, 15, 30, 45, 60 peripartum for estimation of minerals profile. Animals were followed for puerperal events, uterine involution and intervals for postpartum first estrus and conception. The feeding of bypass fat and chelated minerals had significant effect on the time required for expulsion of placenta (3.93±0.24 vs 7.18±0.72 hrs; p Lass Than 0.01), uterine involution (32.75±0.57 vs 37.00±0.56 days; p Lass Than 0.05), intervals for first estrus postpartum (79.05±3.82 vs 100.55±3.47 days; p Lass Than 0.05) and service period (107.10±4.43 vs 133.65±6.04 days; p Lass Than 0.05). The prepartum mean plasma calcium and inorganic phosphorus levels in the buffaloes of both control and treatment groups decreased significantly on the day of calving, and then gradually increased during the days postpartum, again reaching at par with prepartum levels at around day 15-30 postpartum. The mean plasma calcium levels were observed to be higher in treatment than control group at most of the intervals. The mean plasma inorganic phosphorus values on periods closer to calving were apparently higher in treatment group than the control group. Further, the levels during close peripartum period were observed to be apparently higher in untreated subgroup as compared to Stimvet and Exapar alone or its combination subgroups. The mean plasma magnesium concentrations were found to be almost consistent during entire peripartum period studied in both the groups with apparently higher values in treatment than control group at most of the intervals. Very similar trend was also found in subgroups treated with Stimvet and oral Exapar alone or in combination, being little higher in Stimvet injected subgroup.


Author(s):  
Larysa Fedonyuk ◽  
Yaroslav Stravskyy ◽  
Vira Khavtur ◽  
Roman Sachuk

Aim. To establish the effect of acidum succinicum on the activity of enzymes in the blood of cows after calving and the course of uterine involution. Materials and Methods. The study of enzyme activity was carried out in the Central Research Laboratory of I. Horbachevsky Ternopil National Medical University (Certificate of technical competence № 001/18, issued on September 26, 2018, valid until September 25, 2023). Results of the research. After the use of acidum succinicum in the body of cows of the experimental group, the activity of alanine aminotransferase increased by 12,0 % (p≤0,05), and the activity of aspartate aminotransferase, on the contrary, decreased by 30,0 % (p≤0,001), which indicates a positive effect on hepatocytes. Confirmation of this is the Ritis factor, which increased 1,4 times (p≤0,05). All animal experiments were conducted according to with the recommendations of ARRIVE and the British Animal Act 1986 and Directive 2010/63 / EU on the protection of animals for scientific investigation. In the body of cows of the control group, the activity of alanine and aspartateaminotransferases increased, respectively, by 14,0 % (p≤0,05) and 47,0 % (p≤0,001) against a background of a 1,2-fold decrease (p≤0,05) Ritis factor. A decrease in bilirubin content of 56,0 % (p≤0,001) in the body of cows after the use of acidum succinicum and an increase in its content by 2,2 times (p≤0,001) in the body of control cows indicates liver disease, which is accompanied by affection of its parenchyma, which may be a consequence of intoxication of their body in the last trimester of pregnancy. Conclusion. Acidum succinicum, applied to cows after calving, prevents the development of inflammation of the reproductive system, helps to restore reproductive function, reduces the duration of the service period to 80,0±2,0 days, and the insemination index to 1,3


Author(s):  
В.Г. СЕМЕНОВ ◽  
Е.П. СИМУРЗИНА ◽  
А.А. СЕМЕНОВ ◽  
А.В. АЛЬДЯКОВ

Проведено научное исследование с целью выявления наиболее эффективного биоиммуностимулятора. Нами были использованы биопрепараты, разработанные на базе ФГБОУ ВО Чувашская ГСХА – PS-2 и Prevention-N-E, а также широко распространенные в ветеринарной практике ПДЭ и Е-селен. Для исследований были сформированы четыре группы коров (три опытных и контрольная) по 10 голов в каждой и три группы нворожденных телят (две опытные и контрольная). Внутримышечное введение коровам в сухостойный период препаратов PS-2 (1-я опытная группа) и PreventionN-E (2-я опытная группа) по 10,0 мл трижды за 40, 20 и 10 суток до отела, а также ПДЭ и Е-селен (3-я опытная группа) за 20 суток до отела в дозе 20,0 и 10,0 мл соответственно предупреждает болезни послеродового периода. Биопрепараты способствуют сокращению сроков отделения последа на 5,7; 6,4 и 4,8 ч. Вероятность регистрации субинволюции и воспаления слизистой оболочки матки уменьшалась в 3,0 и 2,0 раза (в 1-й и 3-й группах), а во второй опытной группе исключалась. Первая половая охота наступала на 15,4; 21,1 и 13,7 сут раньше, чем в контроле. Произошло сокращение индекса осеменения в 1,2 раза у коров первой и третьей опытных групп и в 1,5 раза у второй опытной. На фоне снижения гинекологических заболеваний в опытных группах значительно сократился сервис-период и возросла оплодотворяемость в одну охоту. Трехкратная инъекция телятам PS-2 (1-я опытная группа) и Prevention-N-E (2-я опытная группа) в дозе 3,0 мл способствовала снижению заболеваемости. В период выращивания в группах выявлены случаи кишечных и респираторных заболеваний телят, заболеваемость составила 50,0 (контроль); 20,0 и 10,0 % соответственно. Выздоровление у телят опытных групп наступало раньше на 1,4 и 4,0 суток, чем в контроле. У телят контрольной группы коэффициент Мелленберга превышал таковой у опытных сверстников: в 3,0 раза – 1-ой опытной и в 9,8 раза – 2-ой. К завершению периода выращивания живая масса телят опытных групп превосходила контрольную на 5,4 и 8,8 кг. Следует отметить, что более выраженным эффектом обладал комплексный иммуностимулятор Prevention-N-Е. Research according to effi ciency of use is carried out the bioimmunostimulators. Were approved the biological products Prevention-N-E, PS-2, which were developed in Chuvash State Agricultural Academy and widespread in veterinary preparations PDE and E-selen. The introduction of preparations Prevention-N-E and PS-2 to cows in patrimonial period in a dose of 10.0 ml is triple in 45-40, 25-20 and 15-10 days prior to a calving, as well as PDE in a dose of 20.0 ml and E-selen - 10.0 ml once in 20 days prior to a calving warns postpartum diseases. Under the infl uence of biopreparations reduce a time of separation of the fetal membranes by 5.7, 6.4 and 4.8 hours , index insemination in 1.2, 1.5 and 1.2 times , service - period by 23.4, 29.9 and 16.0. days , risk of uterine subinvolution and endometritis reduce in 3.0 and 2.0 times, or whatever was excluded, and a fertility in the fi rst estrus raised in 2.0 and 3.0 times.A triple intramuscular injection of Prevention-N-E and PS-2 to calves in a dose of 3.0 ml reduces quantity of diseases. In a growing period in groups were intestinal and respiratory diseases, morbidity was 50.0, 20.0 and 10.0 % respectively. Terms of recovery are reduced by 1.4 and 4.0 days. The safety of calves in all groups was 100 %. Mellenberg’s coeffi cient exceeded in control group on skilled groups in 3.0 and 9.8 times. In the end of growing period a live mass of calves in skilled groups was more than in control group by 5.4 and 8.8 kg. It should be noted, that a complex preparat Prevention-N-Е has more pronounced effect.


Author(s):  
A.J. Dhami ◽  
K.B. Vala ◽  
F.S. Kavani ◽  
S.C. Parmar ◽  
R.J. Raval ◽  
...  

Advanced pregnant Jaffarabadi buffaloes (n=40) of 2-4 parity selected on an organized farm were divided equally into control (routine farm feeding-RFF) and treatment/nutrients supplementation (RFF + bypass fat @ 150-200 g/h/d and ASMM @ 50 g/h/d) groups and were studied from 6 wks prepartum to 8 wks postpartum for plasma profile of steroid hormones and PGF2a metabolites on days -45, -30, -7, 0, +7, +15, +30, +45 and +60 peripartum as well as for puerperal events and postpartum fertility. Half of the buffaloes in both the groups also received parenteral microminerals (Inj. Stimvet 5 ml i/m) twice, 45 days before and on the day of calving. Again half of them were treated with ecbolic (Exapar) 2 boli bid for first 4 days postpartum. The mean plasma progesterone values were maximum (>4 ng/ml) on day 45 prepartum, which declined significantly (p less than 0.01) on day 7 prepartum reached to the basal levels (1 ng/ml) on the day of calving, remained basal till day 15, and thereafter showed a rising trend on days 30, 45 and 60 postpartum. The oestradiol-17b values were at its peak on the day of calving (p less tahn 0.01), showed a rapid fall by day 7 postpartum and remained low till recrudesce of follicular activity around day 45 and 60 postpartum. The levels of cortisol were significantly higher on the day of parturition as compared to values on day 7 pre- and post-partum. The plasma concentration of PGFM was low on day 45 prepartum, which increased gradually and significantly by almost 10-folds to reach peak values on the day of calving in both control and treatment groups and then declined gradually and significantly till day 45 postpartum. The rise was little more in nutrients supplemented group with higher mean values at most intervals peripartum than in control group. The periods for uterine involution, first postpartum estrus, and days open were significantly shorter with higher conception rate in nutrient supplemented group. It is concluded that the peripartum nutrient supplementation in Jaffarabadi buffaloes is beneficial and has positive effect on the postpartum fertility and plasma progesterone and PGFM profile.


2018 ◽  
Vol 53 (3) ◽  
pp. 371-377
Author(s):  
Ana Paula Saldanha Franzoni ◽  
Joana Ribeiro da Gloria ◽  
Anna Luiza Belli de Souza Alves Costa ◽  
Ronaldo Alves Martins ◽  
Thiago Fernandes Amaral ◽  
...  

Abstract: The objective of this work was to describe the effect of genetic composition on body condition score (BCS), on total lactation production, and on metabolic and hormone profiles of Holstein x Gyr cows under the same pre- and postpartum management conditions. The genetic groups ½ (n=8), – (n=10), and ≥⅞ (n=10) Holstein x Gyr (HG) were evaluated within the same farm, and all of them showed typical lactation curves. The time required to reach peak production did not differ between groups. The groups also did not differ as to time to reach the lowest BCS after calving or to recover BCS after calving, nor as to their BCS values in the week of first ovulation, number of weeks required for first ovulation, and service period. However, ½ HG cows had higher BCS at calving, but also greater BCS loss postpartum. Total lactation production and plasma insulin concentrations of – HG cows postpartum were similar to those of cows specifically selected for milk production. Using parameters obtained with Holstein cows to determine management conditions in farms with crossbred cows can have unwanted consequences, since these animals have different metabolic and hormone profiles.


Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.


2021 ◽  
pp. 074873042098363
Author(s):  
Alejandro A. Aguirre ◽  
Roberto A. Palomares ◽  
Aitor D. De Ondiz ◽  
Eleazar R. Soto ◽  
Mariana S. Perea ◽  
...  

Evidence has accumulated over the years indicating that the moon influences some aspects of the reproductive activity in animals and humans. However, little is known about the influence of the lunar cycle on the reproductive performance of cows under tropical conditions, where the environment strongly affects reproduction. This retrospective study was conducted with the aim of assessing the influence of the lunar cycle on some reproductive traits of tropical crossbred cows managed in a pasture-based system. Data from 5869 reproductive records from two commercial farms localized in the Maracaibo Lake Basin of Zulia State, Venezuela, were analyzed. Variables studied were first service conception rate, calving frequency, first postpartum estrous frequency, and pregnancy frequency. In addition to the lunar cycle, the effects of farm, season, and predominant breed were also considered. Data were analyzed using logistic regression and general linear model from SAS. First service conception was affected by lunar phases and predominant breed, but not by farm or season. For frequencies of calving, first postpartum estrus, and pregnancy, there was no main effect of farm, season, and predominant breed, whereas the effect of lunar phases was highly significant. First service conception was significantly greater in waning than in crescent phase of the lunar cycle. Frequencies of calving, first estrus, and pregnancy were highly correlated and showed greater figures around full moon and new moon. In conclusion, lunar cycle influenced first service conception, attaining greater values in the waning phase of the moon cycle. Frequencies of calving, first postpartum estrus, and pregnancy in crossbred cows showed a clear bimodal rhythm, whose greatest values coincided with new moon and full moon.


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