scholarly journals Potential of biochar after the biological activation by native soil microflora

2021 ◽  
Vol 26 (3) ◽  
pp. e2219
Author(s):  
Helena Dvorackova ◽  
Jaroslav Záhora ◽  
Lubica Pospíšilová ◽  
Vítězslav Vlček

Objective. Dehydrogenase activity after the biological activation of biochar by the native soil microorganisms was studied. The main aim was to improve biochar properties by activation and make it more friendly for the soil microflora. Materials and methods. The activation was reached by aerating with the soil solution for two weeks. No special inoculum of microorganisms was applied. The following treatments in four replicates were prepared: conventional raw biochar (BR), activated biochar (BA), mineral fertilizer DAM 390 (NF), and control (C). A statistical test for comparing treatments means (Fisher p≤0.05; program STATISTICA 12.0; StatSoft software Inc., Tulsa, Oklahoma, USA) was used. Results. Statistically significant differences in the dehydrogenase activity between the treatments BR, BA, and C were found. Application of mineral fertilizers had a negative effect and increasing of nitrogen leaching was observed. Conclusions. Activating of biochar is suitable metods for impove soil biota conition compared with convention biochar.

2018 ◽  
Vol 2 (95) ◽  
pp. 26-29
Author(s):  
O.S. Gavrishko ◽  
Yu.M. Olifir ◽  
T.V. Partyka

The results of studies of the change in redox potential in the profile of light gray forest surface-gleyed soil on variants with long-term agricultural use without applying fertilizers and mineral fertilizer system solely compared with the soil under the forest are presented. On the basis of the conducted analyzes it was established, that soil tillage without fertilizer application and with mineral fertilizer solely has a different effect on ROP in the profile. In the soil without fertilization (control) as compared to the forest a moderate oxidizing (514 mV) and slightly oxidizing (437 mV) processes are happening. Prolonged application of mineral fertilizers to the soil (N65R68K68) significantly reduced the redox potential of all genetic horizons compared with forest and control without fertilizers. For the given fertilizer system the highest values of ROP were obtained in arable HEgl and underarable HEgl layers: 426 mV and 416 mV respectively. Redox potential sharply decreases with the depth to 398-311 mV, which characterizes processes occurring in the soil profile, as weakly reducing and close to moderately reducing.


Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 485 ◽  
Author(s):  
Jakub Elbl ◽  
Jana Maková ◽  
Soňa Javoreková ◽  
Juraj Medo ◽  
Antonín Kintl ◽  
...  

The presented paper deals with the analysis of potential differences between organic waste compost (CBD), vermicompost (CVER) and mineral fertilizer (MF; 27% of N) applications affecting the quality of arable soil by influencing microbial activity therein. The selected types of compost represent alternatives to conventional organic fertilizers, which are, however, not available to Czech and Slovak farmers in sufficient amounts. Their mutual comparison and the comparison with organic fertilizers aim to provide farmers further information about their influence on arable land and thus to give them the possibility of deciding on the most suitable amendments. To demonstrate the effect of these amendments, six variants were prepared: one without the addition of fertilizers; two variants with the addition of 40 Mg/ha of CVER and CBD; one variant with the addition of double dosed CVER (80 Mg/ha), and the remaining two variants were fertilized only with MF (0.22 Mg/ha) and with the combination of CVER (0.20 Mg/ha) and MF (0.11 Mg/ha). Substrate induced respiration (SIR), basal respiration (BS), microbial carbon (Cmic) and enzymatic activities (hydrolysis of fluorescein diacetate—FDA, dehydrogenase activity—DHA, and phosphatase activity—PA) were used to evaluate the effect of CBD, CVER and MF application on the soil quality. Both organic and mineral amendments affected BS and SIR. The highest BS and SIR rates were found in variants with compost application (CVER and CBD). All variants treated with the mineral fertilizer showed the lowest level of enzyme activities; lower by about 30% in comparison with variants where CVER, CBD and the combination of MF and CVER were applied. We found insignificant differences between the individual types of compost. More importantly, we compared the situation at the beginning of the experiment and after its end. It was found that the application of mineral fertilizers automatically led to the deterioration of all enzymatic parameters, on average by more than 25%, as compared with the situation at the beginning of the experiment. However, when the mineral fertilizer dose was supplemented with organic amendments (CVER), this negative effect was eliminated or significantly reduced. Furthermore, both composts (CVER and CBD) positively affected plant biomass production, which reached a level of production enhanced by the MF. Results clearly showed that the application of both compost types could be used to improve soil quality in agriculture.


2013 ◽  
Vol 27 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. Jezierska-Tys ◽  
A. Rutkowska

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.


Author(s):  
H.M. Hospodarenko ◽  
◽  
I.V. Prokopchuk ◽  
K. P. Leonova ◽  
V.P. Boyko

The productivity of agricultural crops is the most variable and integral indicator of their vital activity, which accumulates their genetic potential, soil fertility, weather conditions and components of agricultural technology. Soybean under optimal growing conditions (the reaction of the soil is close to neutral, sufficient phosphorus and potassium nutrition, the use of nitraginization) assimilates from the air about 70 % of the total nitrogen requirement. Therefore, it is believed that it is enough to apply only a starting dose of nitrogen fertilizers (20–40 kg/ha a. s.), to get a high yield with good indicators of grain quality. The results of studies of the influence of long-term (8 years) application of different doses and ratios of fertilizers in field crop rotation on podzolized chernozem in the conditions of the Right -Bank Forest-Steppe of Ukraine on the yield and quality of soybean seeds preceded by spring barley were presented. It was found that crop yields could be increased by 18–77 % owing to different doses, ratios and types of fertilizers. The highest indicators of seed yields for three years of the research (3,02 t/ha) were obtained under the application of mineral fertilizers at a dose of N110P60K80 per 1 ha of crop rotation area, including under soybean – N60P60K60. Exclusion of the nitrogen component from the complete fertilizer (N60P60K60) reduced its yield by 26 %, phosphorus – by 17, and potassium by 11 %. There was no significant decrease in soybean yield in the variant of the experiment with a decrease in the proportion of potassium in the composition of complete mineral fertilizer (N60P60K30) for three years of study. The largest mass of 1000 soybean seeds was formed at doses of N60К60 fertilizers, and their protein content — under the application of complete mineral fertilizer in doses of N60P60K60 and N60P60K30.


Author(s):  
Fesenko, H.

Purpose. Increasing the uniformity of distribution of mineral fertilizers and other bulk materials due to the stability of their feed from the body to the spreading working bodies using the top feeder. Methods. The following methods are used to achieve this aim: the method of comparing the differences between individual groups of fertilizers, the method of analyzing the properties of a new technical system, the method of functional inventiveness, and the methods of theoretical and analytical mechanics. Results. The traction body of the conveyor of the upper feed of the body fat body machine for mineral fertilizers and other bulk materials was substantiated and the relationship between the height of its scrapers and the distance between them was established, as well as the nature of the mineral fertilizer pressure on the curvilinear wall of the body. In addition, the design of the advanced body fertilizer spreader is justified, which ensures a stable flow of fertilizers from the body due to the improvement of the top feeder. Conclusions. Because of the conducted researches, the advantages of machines equipped with top feeder are found. They create the conditions for the forced feeding mineral fertilizers and other loose materials from the container to the distribution bodies, which is a prerequisite for their evenness on the surface. With this, the imperfection of known machines with the top feeder constrains their introduction into agricultural production. On this account, a more thoroughly constructed solution of the body feeder of the top feed is substantiated, in which the conveyor provides a stable supply of fertilizers from the body with reduced energy consumption during operation. Keywords: analysis, feed, upper device, conveyor, stability, fertilizers, flow ability, body.


2021 ◽  
pp. 146144482110260
Author(s):  
Ragnhild Brøvig-Hanssen ◽  
Ellis Jones

Many online media platforms currently utilise algorithmically driven content moderation to prevent copyright infringement. This article explores content moderation’s effect on mashup music – a form of remix which relies primarily on the unauthorised combining of pre-existing, recognisable recordings. Drawing on interviews ( n = 30) and an online survey ( n = 92) with mashup producers, we show that content moderation affects producers’ creative decisions and distribution strategies, and has a strong negative effect on their overall motivation to create mashups. The objections that producers hold to this state of affairs often strongly resonate with current copyright exceptions. However, we argue that these exceptions, which form a legal ‘grey zone’, are currently unsatisfactorily accommodated for by platforms. Platforms’ political-economic power allows them, in effect, to ‘occupy’ and control this zone. Consequently, the practical efficacy of copyright law’s exceptions in this setting is significantly reduced.


Author(s):  
Janja Kuzevski ◽  
Nada Milosevic ◽  
Sasa Krstanovic ◽  
Zora Jelicic

In sugar beet production, one of the most important factors that affect the yield, apart from genetic properties, is the use of mineral fertilizers. Considerate amounts of mineral fertilizers are used in sugar beet production. However, if agroecological conditions are not optimum, mineral fertilizers cannot be completely absorbed, which may lead to soil contamination. Therefore, research has been focusing on ways of using atmospheric nitrogen by means of nitrogen-fixing bacteria. Numerous researches have proved that one part of mineral fertilizers can be replaced by biological nitrogen. The aim of this research was to determine the effect of genotype, azotobacter and the amount of mineral fertilizers on the root yield of sugar beet and on the microbiological activity of the sugar beet rhizospheric soil. Three hybrids of sugar beet were used during the two years of the research. The seed of the hybrids was inoculated with three strains of azotobacter. Various amounts of NPK were used (0;30;60;90 kg/ha). At the end of the vegetation period, the following were determined: root yield, total number of bacteria, number of azotobacter, oligotrophic bacteria, ammonifiers, fungi, and actinomycetes in soil. Dehydrogenase activity was measured. The results were processed statistically (analysis of variance for factorial trials) and the effect of the factors was determined upon the expected mean square values. The yield was mainly affected by the amount of mineral fertilizers. However, the effect of mineral fertilizers was different with different inoculation treatments. The effect of the examined factors was dependant upon genotype, amount of mineral fertilizers, inoculation and the year of trials. The interaction between genotype, mineral fertilizers, inoculation and the year of trials was the factor that had the greatest effect on the number of almost all the examined soil microorganisms.


1985 ◽  
Vol 63 (6) ◽  
pp. 1040-1045 ◽  
Author(s):  
J. M. Norton ◽  
G. E. Harman

Responses of soil microorganisms to volatile exudates from germinating pea seeds of differing quality were determined. Germination of sclerotia of Rhizoctonia solani and Sclerotium rolfsii and subsequent hyphal growth were stimulated by exposure to volatiles from aged but not nonaged pea seeds. Hyphae grew preferentially toward aged seeds. In natural soil, bacterial and fungal populations showed significant increases after exposure to volatiles from aged seed. For example, Fusarium spp. and Pseudomonas spp. showed increases of 79 and 2200%, respectively, over their original population levels after a 48-h exposure to volatiles. Conversely, Pythium populations and associated seed-rotting potential of soil decreased in natural soils exposed to volatiles. In autoclaved soils infested with P. ultimum (PHP4), Pythium populations increased dramatically after exposure to volatiles from aged pea seeds. In soils infested with either soil fungi or bacteria in addition to P. ultimum, Pythium levels remained constant or decreased, respectively, with time of exposure. Exposure to the volatiles from aged pea seeds stimulated soil microbial activity. These results suggest that Pythium germlings, when unable to reach a host, are subjected to microbial antagonism in the presence of the native soil microflora. A decrease in cucumber seed rot coincided with decreases in Pythium numbers.


2004 ◽  
Vol 50 (11) ◽  
pp. 957-966 ◽  
Author(s):  
Robert E Andrews Jr. ◽  
Wesley S Johnson ◽  
Abby R Guard ◽  
Jonathan D Marvin

The persistence of Enterococcus faecalis, fecal enterococci from swine waste, and Tn916-like elements was determined following inoculation into autoclaved and native soil microcosms. When cells of E. faecalis CG110 (Tn916) were inoculated into native microcosms, enterococcal viability in the soil decreased approximately 5 orders of magnitude (4.8 × 105CFU/g soil to < 10 CFU/g) after 5 weeks. In autoclaved microcosms, the viability of E. faecalis decreased by only 20% in 5 weeks. In contrast, the content of Tn916, based on PCR of DNA extracts from soil microcosms, decreased by about 20% in both native and autoclaved microcosms. Similar results were obtained when the source of fecal enterococci and Tn916-like elements was swine waste. Because the concentration of Tn916-independent E. faecalis DNA (the D-alanine D-alanine ligase gene), based on PCR, decreased to nearly undetectable levels (at least 3 orders of magnitude) after 5 weeks in the native microcosms, the evidence suggests Tn916 stability in the soil results from en masse transfer of the transposon to the normal soil microflora and not survival of E. faecalis DNA in the soil system. Results from denaturing gradient gel electrophoresis suggest that multiple forms of Tn916 occur in swine waste, but only forms most like Tn916 exhibit stability in the soil.Key words: Tn916, Enterococcus faecalis, soil, antibiotic resistance, conjugation, transposon.


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