scholarly journals First approach to molecular epidemiology of bovine tuberculosis in Colombia

2016 ◽  
pp. 5222-5236 ◽  
Author(s):  
Jimena Jojoa-Jojoa ◽  
Maira Wintaco M ◽  
Francisco Osorio R ◽  
Gloria Puerto-Castro ◽  
Martha Guerrero-Guerrero
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Microbiological Analysis ◽  
Mycobacterium Species ◽  
Molecular Assays ◽  
Nasal Swabs ◽  
The World ◽  
Gross Lesions

ABSTRACT Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.RESUMEN Objetivo. Investigar la presencia de Mycobacterium bovis y otras especies de Mycobacterium en muestras de ganado bovino y búfalino en Colombia e iniciar la caracterización molecular de M. bovis en el país. Materiales y métodos. 492 muestras fueron recogidas de hatos identificados con presencia de animales infectados a través de la prueba de PPD, por el Grupo de Tuberculosis Bovina del Instituto Colombiano Agropecuario ICA en ocho departamentos de Colombia. Se incluyeron ganglios linfáticos de cabeza, tórax y abdomen, tejidos con lesiones macroscópicas de tuberculosis, hisopos nasales, leche, sangre y quesos frescos. Las muestras se sometieron a investigación de Mycobacterium bovis y otras micobacterias mediante el análisis microbiológico convencional y pruebas moleculares de PCR-IS6110 y spoligotyping. Resultados. En las muestras analizadas especialmente en los ganglios linfáticos, se evidenció Mycobacterium bovis con genotipos reportados y no reportados previamente en el mundo, así como M. tuberculosis en los departamentos de Antioquia, Cundinamarca, Magdalena y Boyacá. Conclusiones. En Colombia existen al menos 7 genotipos de M. bovis que se encuentran infectando y enfermando a bovinos y bufalinos de cuatro diferentes departamentos del país constituyéndose en seria amenaza para la salud pública.

scholarly journals Distribution of Lesions in Cattle Infected with Mycobacterium Bovis

1996 ◽  
Vol 8 (3) ◽  
pp. 351-354 ◽  
Author(s):  
Diana L. Whipple ◽  
Carole A. Bolin ◽  
Janice M. Miller
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Dairy Herd ◽  
The United States ◽  
Primary Method ◽  
Thoracic Region ◽  
Gross Lesions ◽  
Tissue Specimens ◽  
Postmortem Examinations

Detailed postmortem examinations were conducted on 30 cattle from a dairy herd with bovine tuberculosis to determine the distribution of lesions in Mycobacterium bovid-infected cattle. Twenty-four different tissue specimens from each animal were examined for gross lesions and collected for bacteriologic culturing and histologic examination. Tuberculosis was confirmed in 15 cattle with evidence of infection in 1 or more of the following tissues: medial retropharyngeal, parotid, tracheobronchial, mediastinal, caudal deep cervical, and subiliac lymph nodes; palatine tonsil; and lung. Gross and histologic lesions were present most frequently in lymph nodes of the thoracic region. Mycobacterium bovis was isolated from 3 cattle that had no gross lesions of tuberculosis. One animal had lesions only in the subiliac lymph node, which is not routinely examined during slaughter surveillance. Results of this study indicate that not all cattle infected with M. bovis have visible lesions of tuberculosis in sites that are routinely inspected. These findings are important because detection of gross lesions of tuberculosis during inspection of carcasses at slaughter is the primary method for detection of tuberculous cattle and herds in the United States.

scholarly journals Gross and Microscopic Lesions of Naturally Occurring Tuberculosis in a Captive Herd of Wapiti (Cervus Elaphus Nelsoni) in Colorado

1992 ◽  
Vol 4 (4) ◽  
pp. 428-433 ◽  
Author(s):  
Jack C. Rhyan ◽  
Dennis A. Saari ◽  
Elizabeth S. Williams ◽  
Michael W. Miller ◽  
Arthur J. Davis ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Cervus Elaphus ◽  
Bovine Tuberculosis ◽  
Caseous Lymphadenitis ◽  
Infected Herd ◽  
Cervus Elaphus Nelsoni ◽  
Naturally Occurring ◽  
Gross Lesions ◽  
Immunohistochemical Techniques

A Mycobacterium bovis-infected herd of captive wapiti (Cervus elaphus nelsoni) in Colorado was depopulated after lesions of bovine tuberculosis were confirmed in 8 of 10 tuberculin skin test reactors. Of the 43 animals > 1 year of age, 26 had gross lesions suggestive of tuberculosis, 24 had microscopic lesions of tuberculosis, and 23 had acid-fast bacilli associated with the lesions. Lungs and retropharyngeal lymph nodes were the most frequently affected sites. Most lesions grossly and microscopically resembled tuberculosis in cattle; however, some lesions resembled abscesses or ovine caseous lymphadenitis lesions. Special stains and immunohistochemical techniques labeled few to numerous mycobacteria associated with the lesions.

scholarly journals Prevalence of Mycobacterium bovis in some cattle breeds in the aids era: An abattoir survey in Ogun State, Nigeria

2020 ◽  
Vol 40 (2) ◽  
pp. 209-215
Author(s):  
M. A. Oyekunle ◽  
A. O. Talabi
Keyword(s):  
Growth Rate ◽  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Standard Method ◽  
Cell Morphology ◽  
Seasonal Distribution ◽  
Biochemical Characteristics ◽  
Ogun State ◽  
Lowenstein Jensen ◽  
Gross Lesions

Eight breeds of cattle totalling 15520 heads and comprising of 3784 bulls and 11736 cows were examined during slaughter in the two largest abattoirs in Ogun State, Nigeria between January and December, 2007. Samples of lung, liver, spleen, kidney, udder, skin and pleural lymph nodes with gross lesions suspected to be those of tuberculosis were obtained from the slaughtered animals. Each of the samples was processed according to the standard method and thereafter stained by Ziehl-Neelsen’s technique and was microscopically examined for acid fast organisms. Samples containing acid fast bacilli were cultured on Lowenstein-Jensen (LJ) medium with pyruvate or glycerol. M. bovis was identified by growth rate, pigment production, colony and cell morphology and biochemical characteristics. A total of 41 heads of cattle comprising of 9 bulls and 32 cows from 7 breeds were positive for M. bovis. No isolate of M. bovis was obtained from Keteku breed and no seasonal distribution of the organism was observed. Among the organs with lesions, the lung had the highest number (18) of M. bovis. The number of White Fulani cattle with M. bovis was highest (13) among the breeds examined.

scholarly journals SARS-CoV-2 Virologic and Immunologic Correlates in Patients with Olfactory and Taste Disorders

2020 ◽  
Vol 8 (7) ◽  
pp. 1052
Author(s):  
Marco Benazzo ◽  
Irene Cassaniti ◽  
Eugenia Maiorano ◽  
Anna Calastri ◽  
Federica Novazzi ◽  
...  
Keyword(s):  
Median Time ◽  
Crucial Role ◽  
Great Majority ◽  
Molecular Assays ◽  
Nasal Swabs ◽  
Taste Disorders ◽  
Over Time

The main object of the study was to investigate the SARS-CoV-2 molecular and serological pattern in patients with mild symptoms including anosmia and ageusia. A cohort of 69 patients with olfactory and taste disorders (OTDs) were enrolled and prospectively monitored. Serological and molecular assays for the characterization of SARS-CoV-2 IgG and SARS-CoV-2 RNA, respectively, were performed at the time of enrolment and after 7 and 14 days. Patients were stratified according to the symptoms’ onset. A total of 52 patients (75.4%) were diagnosed as COVID-19 positive being SARS-CoV-2 RNA and/or SARS-CoV-2 IgG positive. The remaining 17 (24.6%) were negative for COVID-19 and excluded from the analysis. We reported that only 34 out of 52 patients (65.4%) were positive for SARS-CoV-2 RNA. Moreover, the median time from onset of symptoms and enrolment was significantly higher in those patients with negative SARS-CoV-2 RNA in nasal swabs, suggesting that symptoms might last longer than SARS-CoV-2 replication. The great majority of patients (80%) developed SARS-CoV-2 IgG at three weeks after symptoms’ onset while the detectability of SARS-CoV-2 RNA dramatically decreased over time, suggesting the crucial role of combination of molecular and serological assays for the diagnosis of COVID-19 in those patients reporting mild symptoms.

scholarly journals Bovine tuberculosis in domestic and wild mammals in an area of Dorset. III. The prevalence of tuberculosis in mammals other than badgers and cattle

1982 ◽  
Vol 89 (2) ◽  
pp. 225-234 ◽  
Author(s):  
T. W. A. Little ◽  
C. Swan ◽  
H. V. Thompson ◽  
J. W. Wilesmith
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Rattus Norvegicus ◽  
Bovine Tuberculosis ◽  
Vulpes Vulpes ◽  
Wild Mammal ◽  
Wild Mammals ◽  
Large Sample

SUMMARYA large sample of the wild mammals found on a farm in South Dorset were trapped and examined for the presence ofMycobacterium bovisfollowing the discovery of widespread infection in cattle and badgers.M. boviswas isolated from the lymph nodes of two out of 90 rats (Rattus norvegicus) and one out of seven foxes (Vulpes vulpes) but no lesions of tuberculosis were observed. It was concluded that the badger was the only species of wild mammal which was a reservoir ofM. bovisin this area.

scholarly journals Proposal of a Screening MIRU-VNTR Panel for the Preliminary Genotyping ofMycobacterium bovisin Mexico

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Enrique Bolado-Martínez ◽  
Iliana Benavides-Dávila ◽  
Maria del Carmen Candia-Plata ◽  
Moisés Navarro-Navarro ◽  
Magali Avilés-Acosta ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Causative Agent ◽  
Bovine Tuberculosis ◽  
Allelic Diversity ◽  
Geographical Regions ◽  
Wide Range ◽  
The World ◽  
Allelic Variability ◽  
High Discriminatory Power ◽  
Geographic Regions

Mycobacterium bovisis the major causative agent of bovine tuberculosis, one of the most relevant zoonoses in the world, and affects a wide range of wild and domesticated animals. Development of screening panels in mycobacterial genotyping, according to specific geographical regions, is strongly needed. The aim of this study is to select a panel, constituted by highly polymorphic MIRU-VNTRloci, to discriminate clinical isolates ofM. bovisin Mexico. In this study, 65 isolates ofM. bovisobtained from clinical bovine samples proceeding from different geographic regions of Mexico were identified by phenotypic and genotypic tests and subsequently genotyped by a 24-locusMIRU-VNTR panel. The most polymorphiclociwere selected to build a panel with a high discriminatory power similar to the 24-locuspanel results. A panel of seven elements (QUB 11a, MIRU 26, ETR-A, QUB 26, MIRU 16, MIRU 27, and MIRU 39) with the highest allelic diversity showed an appropriate differentiation. The selected MIRU-VNTR elements, according to the regional allelic variability, may be used in the preliminary genotyping ofMycobacterium bovisisolates in Mexico.

scholarly journals Isolation and identification of Mycobacterium bovis in cattle slaughtered from an abattoir in Garanhuns, Pernambuco

2018 ◽  
Vol 39 (1) ◽  
pp. 157 ◽  
Author(s):  
Stephanie Caroline Gueiros Silva ◽  
Amanda De Deus Ferreira Alves ◽  
Tássio José de Oliveira Almeida ◽  
Pamella Christhine Lins de Sá ◽  
Antonio Franciso de Souza Filho ◽  
...  
Keyword(s):  
Public Health ◽  
Bovine Tuberculosis ◽  
High Rate ◽  
Histopathological Analysis ◽  
Isolation And Identification ◽  
Bacteriological Diagnosis ◽  
Lowenstein Jensen ◽  
Gross Lesions ◽  
Hematoxylin And Eosin

Tuberculosis is a chronic infectious disease caused by the Mycobacterium tuberculosis complex (CMT), members including M. bovis. The sanitary inspection in slaughterhouses has a great importance on public health for the removal of contaminated meat with pathological lesions. Cattle slaughtered in the abattoir of Garanhuns were subjected to macroscopic, histological, bacteriological, and molecular analyses. Lung inspection revealed gross lesions suggestive of tuberculosis. The characterization of tuberculous granulomas was performed by histopathology and hematoxylin and eosin (HE) staining. The identification of acid-fast bacilli (AFB) was performed on smears with fresh material and histological staining using Ziehl-Neelsen stain. Bacteriological diagnosis was carried out using Stonebrink and Lowenstein-Jensen media, and polymerase chain reaction (PCR) was performed from the DNA extracted from colonies for the identification of Mycobacterium spp. We found that 1% (32/3,.180) of the collected lungs showed lesions suggestive of tuberculosis. Histopathological analysis revealed that 65.62% (21/32) samples had granulomatous pneumonia. AFB was detected in 46.88% (15/32) smears and 4.16% (1/24) histological sections, as analyzed by Ziehl-Neelsen staining. Moreover, 68.75% (22/32) samples showed bacterial growth and molecular identification 90% (18/20) of the samples were positive for M. bovis and 10% (2/20) for Mycobacterium sp. Thus, microbiological culture and PCR analyses revealed the high rate of bovine tuberculosis in this region, indicative of the need to disclose these results in the interest of public health. Consequently, we emphasize the need for an accurate macroscopic evaluation of lesions suggestive of bovine tuberculosis.

scholarly journals Bovine Tuberculosis in the Republic of Macedonia: Postmortem, Microbiological and Molecular Study in Slaughtered Reactor Cattle

2017 ◽  
Vol 40 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Iskra Cvetkovikj ◽  
Slavcho Mrenoshki ◽  
Kiril Krstevski ◽  
Igor Djadjovski ◽  
Branko Angjelovski ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Bovis ◽  
Molecular Identification ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Nucleotide Polymorphisms ◽  
Republic Of Macedonia ◽  
Mycobacterium Caprae ◽  
Lowenstein Jensen ◽  
The Republic

Abstract Bovine tuberculosis is a chronic infectious disease in cattle caused mainly by Mycobacterium bovis and to a lesser extent by Mycobacterium caprae. The other members of the Mycobacterium tuberculosis complex (MTBC) can also cause the disease in domestic and wild animals and all of them have a zoonotic potential. The main purpose of the study was to determine the presence and distribution of the tuberculous lesions in reactor cattle, and to isolate and identify the causative agents of bovine tuberculosis in the Republic of Macedonia. Lymph nodes and affected organs from 188 reactor cattle slaughtered due to a positive intradermal comparative cervical tuberculin test were analyzed by detection of tuberculous lesions, followed by isolation and molecular identification of the isolated mycobacteria. The isolation was performed on selective media - Lowenstein Jensen with glycerol, Lowenstein Jensen without glycerol and Stonebrink medium supplemented with pyruvate. The molecular identification of the MTBC members was performed by analysis of the Regions of difference (RD1, RD9 and RD4) and detection of single nucleotide polymorphisms in the lepA gene for Mycobacterium caprae. Typical tuberculous lesions were detected in 62 animals (33.0%) and the lesions were most prevalent in the mediastinal lymph nodes (47.5%). The isolated mycobacteria in the MTBC were identified as Mycobacterium bovis and Mycobacterium caprae and were found in both animals with visible lesions (82.2%) and animals without visible lesions (27.7%). The slaughterhouse postmortem examinations and laboratory investigations should be included on regular bases in order to improve the National eradication program.

scholarly journals Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Elizabeth Hortêncio de Melo ◽  
Harrison Magdinier Gomes ◽  
Philip Noel Suffys ◽  
Márcia Quinhones Pires Lopes ◽  
Raquel Lima de Figueiredo Teixeira ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Essential Role ◽  
Allelic Diversity ◽  
Infectious Agent ◽  
The State ◽  
Dairy Herds ◽  
Genotypic Characterization ◽  
Epidemiological Tool

Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture, and colonies compatible with Mycobacterium spp. were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study on the genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this epidemiological tool being a determinant in the rigor of the sanitary practices of disease control in dairy herds.

scholarly journals Evaluation of classical and rapid methods for isolation and identification of Mycobacterium bovis in cattle in Bulgaria

2021 ◽  
Vol 24 (3) ◽  
pp. 334-343
Author(s):  
T. Savova-Lalkovska ◽  
M. Bonovska ◽  
A. Dimitrova ◽  
V. Valcheva ◽  
Y. Petkov ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Economic Impact ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Bacterial Isolates ◽  
Tuberculosis Complex ◽  
Isolation And Identification ◽  
Rapid Methods ◽  
Diagnostic Capabilities

Bovine tuberculosis is still a serious problem with major economic impact in many countries. The aim of study was to evaluate the diagnostic capabilities of the classical and some modern, rapid methods for isolation and identification of Mycobacterium bovis. In the period 2015-2018 from 29 outbreaks in 10 different regions of Bulgaria, 1193 lymph nodes from slaughtered cattle were examined by pathoanatomical, bacteriological, PCR and immunochromatographic methods. Of the 283 bacterial isolates, 263 were identified as M. bovis - member of the M. tuberculosis complex.

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